수용성 키토산 유도체를 이용한 방사성 스트론튬의 시험관내 흡착능 및 마우스내 체외배출 효과

김희경,송해미,조덕제,조만기,이정석,이응호 동서대학교 부설 연구소 1996 연구소 논문집 Vol.1 No.-

키토산은 천연착화제로서 효용가치가 높은 무독성의 천연고분자로서 키틴으로부터 탈아세틸화시킨 유도체이다. 키토산에 인산기을 도입한 Water-solubel phosphorylated Chitosam(WSPC)의 Sr에 대한 시험관내 흡착률은 pH 3에서는 97.8%이었으나, pH 5와 pH 7에서는 모두 100%이었다. WSPC를 농도별(0.01%, 0.1%, 1%)로 희석하여 11주령의 ICR 수컷 마우스의 위내로 투여한 직후 Sr을 같은 경로로 처리한 다음, WSPC의 Sr에 대한 체외 배출효과와 골내 잔존률을 평가하였다. 또한 Sr와 농도별 WSPC 처리후 6일째에 척추, 두개골, 대퇴골, 경골, 치아 및 꼬리뼈 등에 대해 Sr 잔존율을 측정하였다. 0.01-0.1% 사이의 WSPC를 접종한 마우스 투여군이 Sr만 경구 투요한 대조군에 비하여 골조직에 잔존하는 Sr의 침착률이 낮게 나타났다.(P<0.01). 또한 방사성 스트론튬의 분뇨를 �蔥�배출 효과 역시 WSPC 처리군이 대조군에 비해 유의성이 인정되었고(P<0.05), 대체로 WSPC 농도가 높을수록 그 효과는 비례하였다.(P<0.05). 본 실험에 사용된 WSPC는 체내에 유입된 Sr을 제거하는데 효과가 인정되어 방사성 스트론튬의 방어약제중 하나로 활용할 수 있다고 판단되었다.

위암환자의 생존율에 관한 역학적 조사연구

김덕성,이태용,이영수,류기하 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

To find out the survival rates of stomach cancer patients, the author studied 288 stomach cancer patients who took operation at the Department of Surgery in Chungnam National University Hospital from January 1, 1985 to December 31, 1991. The results were as follows: 1. Of the total patients, 67.4% were male: but 32.6% were female. 2. 64.6% of patients lived in rural area and 35.4% urban;98.3% of those were married; 30.6% were farmers and 17.4% were commercial workers; 54.5% were smokers; 48.3% were alcohol drinkers. 3. By types of gastric cancer, 84.4% were progressive gastric cancer patients. Among these, type 2 patients were more than other type patients. 61.5% of those had metastasis to lymph node. 55.2% of those took operation by RSG with gastrojejunostomy; 97.6% of those knew that they had cancer; 19.1% of those had a family history that at least one of family members had cancer. 4. The gross 5 year survival rate of total patients was 47.2%. 5. The 5 year survival rate of male patients was 41.6% but that of female was 46.3%. The 5 year survival rates by residential area, chemotherapy, marital status, blood type, family history, smoking, and drinking were not significantly different. 6. The 5 year survival rate of type 2 progressive gastric cancer patients was the highest with the rate of 72.0%. Metastasis to lymph node and type of operation showed statistically significant difference in 5 year survival rates.

꽃노랑총채벌레(Frankliniella occidentalis)의 외부 미세구조의 특징

김기덕,김남성,박수진,서미자,채순용,윤영남 충남대학교 농업과학연구소 1999 농업과학연구 Vol.26 No.2

SEM을 사용하여 꽃노랑총채벌레, Franklimella occidentalis의 외부형태적 특징을 살펴보았다. 꽃노랑총채벌레의 촉각은 모두 8마디로 구성되어 있으며 길이는 0.25㎜정도이다 또한 3번째 마디와 4번째 마디는 부메랑 모양을 하고 있으며 5번째와 6번째 마디에는 길이가 7㎛정도의 감각기가 있다. 8번째마디에는 8개의 감각기가 있다. 입틀은 비교적 앞으로 튀어나온 모양을 하고 있으며 많은 감각기가 분포하고 있는데 모두 14개의 감각기가 양쪽으로 배열되어 있다. 다리의 구조는 발톱을 가지고 있지 않다. The external morphology of Frankliniella occidentalis was studied by using the scanning electron microscopy (SEM). The antenna was consisted of 8 segments and the length of antenna was 0.25mm. The 3rd segment and 4th segment were bent. The 5th segment and 6th segment had sensilla with 7㎛ long. The 8th segment had 8 sensilla. The mouthpart was protruded and distributed many sensilla on there. The leg had no claw.

주사전자현미경을 이용한 온실가루이(Trialeurodes vaporarium) 성충 부속지의 외부 미세구조 관찰

서미자,김기덕,김남성,박수진,채순용,윤영남 충남대학교 농업과학연구소 1999 농업과학연구 Vol.26 No.2

온실가루이의 외부형태적 특징을 주사전자현미경 사진을 통해 관찰하였다. 우선 이들은 포탄모양의 알을 산란하는데 짧은 갈고리모양의 구조에 의해 잎 뒷면에 붙어있는 것이 확인되었다. 촉각의 길이는 0.3㎜로 전체가 6마디로 구성되어 있고, 첫마디는 굵고 짧으며 둘째 마디부터 가늘고 긴 채찍모양을 하고 있다. 끝마디인 6번째 마디의 끝에는 길이가 10㎛정도의 가늘고 비교적 긴 감각기를 가지고 있어 기주를 탐색하는 과정에서 식물체의 표면을 탐지하는데 사용하는 것으로 사료되며, 바로 밑부분에는 길이가 7-8㎛정도의 비교적 굵은 감각기 2개가 존재하고 있는 것으로 확인되었다. 이 감각기는 반대편에는 존재하고 있지 않은 비대칭 분포를 보이고 있으며, 또한 두개의 모양이 서로 상이함으로 볼 수 있다. 이들의 기능은 주로 기주식물을 탐색하거나 후각기능의 화학적 감각기의 역할을 하는 것으로 추정할 수 있다. 섭식행동이 이루어지는 온실가루이 구기는 전형적인 piercing-sucking type으로, 구침이 길게 잘 발달되어 있다. 구기의 끝부분은 10여개의 미세감각기가 분포고 있어 식물체 표면을 탐색하고 맛을 보는 미각기능을 하고 있으리라 추측할수 있다. 구침은 종종 구기에서 분리되기도 하지만, 구침을 잡아주는 지지대가 기부쪽에 자리하고 있음을 알 수 있다. 구침은 두 개의 반원형이 붙어 있는 형상을 하고 있으며, 다른 흡즙형 구침과는 달리 톱날모양의 구조를 볼 수 없었다. 한편 온실가루이의 다리 끝에 붙어 있는 발톱은 식물체에 고정시키기 위한 구조로 보이는 두 개의 갈고리 형태의 발톱과 그 사이에 중앙지지대 형태의 구조인 paranychium을 포함하여 3갈래의 구조를 하고 있다. External morphology characteristics of the greenhouse whitefly, Trialeurodes vaporarium were investigated by scanning electron microscopy. The antennae of the greenhouse whitefly was 6 segments, rod-shape, and 0.3mm length. On the 6th segment, there were many sensilla for searching host-plant as olfactory receptor. The mouthpart of the greenhouse whitefly was a piercing-sucing type, then its stylet was well developed for piercing plant leaf tissue. Claw of the foreleg was a 3-war hook shape including paranychium for attaching plant surface to pierce and lay egg.

소 傳染性鼻氣管炎 바이러스에 대한 monoclonal antibody 生産과 診斷法 開發 : Ⅱ. Monoclonal antibody를 이용한 소 傳染性鼻氣管炎의 診斷 Ⅱ. Diagnosis of infectious bovine rhinotracheitis by using monoclonal antibody

全茂炯,金德煥,安壽煥,李重馥,閔元其 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-

To develop more specific and sensitive diagnostic methods for infectious bovine rhinotracheitis, 7-C-2 monoclonal antibody specific to polypeptides of infectious bovine rhinotracheitis virus (IBRV) was applied in indirect immunofluorescence antibody assay(IFA), indirect immunoperoxidase assay(IPA) and radial immunodiffusion enzyme assay (RIDEA). It was found that IBRV infected in MDBK cells could be detected as early as 8 hours post infection by IFA, and that IFA was more rapid and specific to identify IBRV antigen than IPA. The diagnostic efficacy of RIDEA and SN test was studied with 88 bovine sera. It was evident that RIDEA could eliminate the false positive reaction encountered in serum neutralization(SN) test, being more rapid and sensitive than the latter. Highly significant correlation coefficiency(r=0.76, p<0.01) was evaluated between the titers of sera and the diameters of RIDEA. Tracheal membranes and sera collected from 96 slaughtered cattle with lesions in respiratory organs were examined to detect IBRV antigen and antibody by IFA, RIDEA and SN test. It was presented that positive rates were 32.3% in IFA, 20.8% in RIDEA and 21.9% in SN test, and that coincidence rate between RIDEA and SN test were 100% in positive sera and 98.7% in negative sera. In conclusion, it was assumed that application of monoclonal antibody could improve the diagnostic efficacy of IBR by enhancing sensitivity and specificity of IPA, IFA and RIDEA.

새로운 미세구형 탄소 입자의 합성에 관한 연구

서길수,이종두,김기덕 嶺南大學校 工業技術硏究所 1991 연구보고 Vol.19 No.1

The dispersion polymerization of phenol with formalin led to microspheric phenolic resin. The surface properties of these polymers were compared with those of microspheric resoroinolic resin, and it showed that it was possible to prepare microspheric carbon via pyrolysis of microsperic phenolic resin. In order to increase the electrical conductivity of microspheric carbon, we also tried to make metal-coated carbonic microsphere by nonelectrolytic metal coating process.

Identification and characterization of homeobox (Hox) gene clusters in harpacticoid and calanoid copepods

Duck-Hyun Kim,Haksoo Jeong,Min-Sub Kim,Eunjin Byeon,Sanghee Kim,Sami Souissi,Heum Gi Park,Atsushi Hagiwara,Jae-Seong Lee (사)한국해양생명과학회 2021 (사)한국해양생명과학회 학술대회 Vol.2021 No.11

Palladium Nanoparticle Catalyzed Conversion of Iron Nanoparticles into Diameter- and Length-Controlled Fe₂P Nanorods

Kim, Heonjo,Chae, Youngjoo,Lee, Duck Hyun,Kim, Minsik,Huh, Jiyoung,Kim, Youngki,Kim, Hyunjin,Kim, Hyun Jung,Kim, Sang Ouk,Baik, Hionsuck,Choi, Kihang,Kim, Jong Seung,Yi, Gi-Ra,Lee, Kwangyeol WILEY-VCH Verlag 2010 Angewandte Chemie Vol.49 No.33

<B>Graphic Abstract</B> <P>Playing a dual role as a catalyst that destabilizes Fe nanoparticles to form soluble precursors in situ and as a catalytic center for nanorod growth allows Pd nanoparticles to transform Fe nanoparticles and a P source into Fe<SUB>2</SUB>P nanorods (see scheme). The diameter and length of the Fe<SUB>2</SUB>P nanorods can be fine-tuned by means of the diameter of the Pd nanoparticles and the Fe/Pd ratio, respectively. <img src='wiley_img_2010/14337851-2010-49-33-ANIE201001822-content.gif' alt='wiley_img_2010/14337851-2010-49-33-ANIE201001822-content'> </P>

The effect of dentin surface treatments on durability of resin-dentin bond

Duck-Su Kim,Sun-Young Kim,Sang Hyuk Park,Gi-Woon Choi,Kyoung-Kyu Choi 대한치과보존학회 2010 Restorative Dentistry & Endodontics Vol.35 No.S2

Studies on the optimal conditions for a high yield of Ganoderma lucidum in Korea

Kim Hong-Kyu,Lee Hee-Duck,Kim Yong-Kyun,Han Gyu-Hueng,Kim Hong-Gi 한국자원식물학회 2000 한국자원식물학회 학술심포지엄 Vol.2000 No.-

Narcotic drugs generally refer to serious and habitual hidden rash such as opium, heroin, methyphetamin, nabinol, cocaine, and so forth. At present, narcotic drugs are spread unchecked and are causing a big social problem. So many countries and narcotists are making every effort to set up a barricade against narcotic drugs. And there is a limit suitable treatment for them. Thus Tuo Yin Tang hang is developed. As indicated by Chinese letters, Tuo yin Tang Jiang(TYTJ) is a crude drug. It is a traditional chinese medicine developed by the study done from lune in 1998 to lune in 1999 that Hubeisheng was entrusted with by People's Republic of China. This study is a treatise on etiology and syntomatology or narcotism. TYTJ is a medicine which is in accord with Pharmacopeia of the People's Republic of China in order to remove from the body the toxic materials resulting from narcotic drugs such as opium and heroin. According to the standard diagnosis on narcotism, 105 cases are studied and treated at the Rehabilitation Center attached to Enshi Autonomous Region Hospital in Hubeisheng. 105 cases are divided into 2 groups by double-blind method. One is the experimental group which has 56 cases. The other is the control group which has 49 ones. 13 cases among 105 cases are addicted by intravenously injections. 9 cases are by oral takings. It took 10 days for this experiment to be performed. Two groups didn't show a striking individual variation based on the age, gender, period of taking drugs, withdrawal symptoms, complication, and state of health. The experimental group had a higher effect of treatment than the control group had. TYTJ treats diseases effectively and has no side effect, irrespective of the serious or slight addiction to opium and morphine.