Diagnostic Availability of Estrogen Receptor Alpha mRNA on Cervical Cancer Tissue

Geehyuk Kim,Kwangmin Yu,Jungho Kim,Seoyong Kim,Sunyoung Park,Sungwoo Ahn,이지영,Sunghyun Kim,박호현,이동섭 대한임상검사과학회 2018 대한임상검사과학회지(KJCLS) Vol.50 No.4

Cervical cancer is the fourth most frequently diagnosed cancer in women worldwide. In lower Human Development Index countries, it has the second highest incidence and mortality among cancer in women. Therefore, better diagnosis and treatment systems are needed. Among them, estrogen receptor alpha (ER-α) mRNA expression has been analyzed with RT-qPCR since several studies reported that ER-α is necessary in the maturation of the uterus and is related to cervical cancer. In this study, ER-α quantitative analysis was performed on various lesions and normal tissue samples. Based on the receiver operating characteristic (ROC) curve, its sensitivity and specificity were 85% and 75%, respectively, showing higher or similar results to those of conventional HPV tests. In addition, its expression level was analyzed with clinical information. With regression analysis, the R square value between the ER-α mRNA expression level and menopause status was 0.5041, indicating a strong correlation. This study was performed as part of a pilot study and suggests that ER-α is related to carcinogenesis. Future studies will examine other hormones and menopausal factors with a larger sample size.

Distribution of Oncogenic Human Papillomavirus Genotypes at High Grade Cervical Lesions above CIN 2 Grade with Histological Diagnosis

Geehyuk Kim,Sungyoung Park,Hye-young Wang,Sunghyun Kim,Sangjung Park,Kwangmin Yu,Boohyung Lee,Seung-Ju Ahn,Eun-Joong Kim,Dongsup Lee 대한의생명과학회 2016 Biomedical Science Letters Vol.22 No.2

High risk human papillomavirus (HR-HPV) is major risk factor for uterine cervical cancer. There are approximately 15 types of HR-HPV. Liquid based cytology samples (116 samples) with high grade cervical lesions belonging to cervical intraepithelial neoplasia (CIN) 2, CIN 3, carcinoma in situ (CIS) and squamous cell carcinoma (SCC) were used after histologic confirmation. HR-HPV genotype assay was conducted using DNA chips. The HR-HPV infection rate was 81.9% with SCC samples showing the highest HR-HPV infection rate of 31%. CIN 3, CIS and CIN 2 showed infection rates of 25%, 16.4% and 9.5%, respectively. According to age with HR HPV infection rate, the 30~39 years-old group showed the highest infection rate by 92.3%. According to distribution with HR HPV genotyping, HPV 16 showed the highest infection rate by 42.3% whereas HPV 33 and HPV 58 showed infection rates of 11.7% and 10.8%, respectively. HPV 18 which is the second most common infected HPV genotype in the world showed 3.6%. Of the three most common oncogenic HR-HPV genotypes in CIN 2, we detected HPV 16, 35, 58; CIN 3 was HPV 16, 33, 58; CIS was HPV 16, 58, 33 (35/52); and SCC was HPV 16, 33, and 18 (31/52/58). Among the HPV 18, CIN 2, CIN 3, CIS and SCC showed 0.9%, 0.9%, 0% and 1.8%, respectively. The most often used preventive vaccines for cervical cancers use HPV 16 and HPV 18 as targets. However, results derived from this study suggest that a preventive vaccine against HPV 16 and HPV 18 would not be optimal for populations in this study.

Clinical Evaluation of Human Papillomavirus DNA Genotyping Assay to Diagnose Women Cervical Cancer

Sunghyun Kim,Dongsup Lee,Yeun Kim,Geehyuk Kim,Sangjung Park,Yeonim Choi,Tae Ue Kim,Kwang Hwa Park,Hyeyoung Lee 대한의생명과학회 2012 Biomedical Science Letters Vol.18 No.2

In this study, we evaluated the human papillomavirus (HPV) genotyping test called MolecuTech REBA HPV-ID® (YD Diagnostics, Seoul, Korea) for 704 women who also had cervical cytological evaluations by Thin Prep. The infection rate of high-risk HPV genotypes was 56.6% in patients with normal cytology, 59.8% in those with benign, low-grade squamous intraepithelial lesions, 51.4% in those with atypical squamous cells of uncertain significance, 92.3% in those with high-grade squamous intraepithelial lesions, and 94.1% in those with squamous cell carcinoma or adenocarcinoma. HPV 16 was the most common genotype detected in any lesion, followed by HPV 53, 58, 33, 52, 45, 31, and 35, in order. The HPV DNA test with PCR-REBA is a very highly sensitive, but less specific, method. The infection rates and HPV genotype distribution of non-Korean people versus people from South Korea showed regional differences.

중국과 한국여성에서 나이에 따른 인유두종바이러스의 유병률과 분포

김지혁 ( Geehyuk Kim ),김성현 ( Sunghyun Kim ),박선영 ( Sunyoung Park ),박상정 ( Sangjung Park ),( Han Lin ),( Yubo Ren ),( Li Yingxue ),이인수 ( In Soo Lee ),이재달 ( Jae Dal Lee ),이동섭 ( Dongsup Lee ) 대한임상검사과학회 2015 대한임상검사과학회지(KJCLS) Vol.47 No.4

Cervical cancer is the third most common cancer among women worldwide. Cervical cancer is significantly associated with human papillomavirus (HPV) infection. The prevalence of HPV infection is influenced by geography, immune status, sexual history and genetic factors. For example, geographically, HPV prevalence varies from 1.5% to 39%. However, little is known about the relationship between HPV prevalence and age. An analysis of HPV prevalence by age will help determine when high-risk groups are exposed to HPV. Such an analysis could also demonstrate a correlation between specific HPV genotypes and age. In addition, the analysis might clarify the optimum age for using vaccines. In this study, HPV prevalence and genotype distribution among Korean and Chinese women are analyzed by age. The REBA HPV-IDⓡ assay (YD diagnostics, Yong-in, Republic of Korea) was used for detecting HPV genotypes in uterine cervical liquid-based cytology samples from 533 women from Korea and 324 from East China (Western Shandong province. Women with severe dysplasia such as SCC (Squamous cell carcinoma) and HSIL (High-grade squamous intraepithelial lesion) groups were primarily in their 40s and 50s, whereas women with mild and moderate dysplasia (ASCUS and LSIL groups) were primarily in their 30s and 40s. Women with HPV genotype 16 and 18 infections were primarily in their 40s. The results suggest that HPV infection is associated with certain age groups in the Korean population.

Circulating miR-221 and miR-222 as Potential Biomarkers for Screening of Breast Cancer

Jungho Kim,Sehee Oh,Sunyoung Park,Sungwoo Ahn,Yeonim Choi,Geehyuk Kim,Seung Il Kim,Hyeyoung Lee 대한의생명과학회 2019 Biomedical Science Letters Vol.25 No.2

Breast cancer is the second most common cancer in women with approximately 522,000 deaths annually worldwide. microRNAs have recently been studied as potential biomarkers that regulate gene expression and are involved in tumorigenesis. Here we evaluated circulating miR-221 and miR-222 as potential biomarkers for breast cancer by quantitative reverse transcription PCR using blood plasma of 30 healthy controls and 30 breast cancer patients. The TNM stage on circulating miR-221 and miR-222 was also investigated. Circulating miR-221 and miR-222 were significantly up-regulated in breast cancer patients compared to those in healthy controls (P < 0.0022 and P = 0.0058, respectively). Furthermore, the relative expression level of circulating miR-221 in patients with stage III breast cancer was higher than in those with stage I and II. Taken together, we have shown circulating miR-221 and miR-222 could be useful biomarkers for the screening of breast cancer patients.

Use of hTERT and HPV E6/E7 mRNA RT-qPCR TaqMan Assays in Combination for Diagnosing High-Grade Cervical Lesions and Malignant Tumors

Wang, Hye-Young,Park, Sunyoung,Kim, Sunghyun,Lee, Dongsup,Kim, Geehyuk,Kim, Yeun,Park, Kwang Hwa,Lee, Hyeyoung American Society for Clinical Pathology 2015 American journal of clinical pathology Vol.143 No.3

<P>Objectives: Human papillomavirus (HPV) is a major cause of cervical cancer, which is the second most common cancer in women. HPV E6 initiates degradation of cellular tumor suppressor protein p53, induces human telomerase reverse transcriptase (hTERT) activity, and then leads to progressive cervical carcinogenesis. Methods: In this study, the CervicGen HPV RT-qDX assay (Optipharm, Osong, Republic of Korea), which detects 16 HPV high-risk subtypes (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, and 69), and the CervicGen hTERT RT-qDX assay (Optipharm) were evaluated using 545 ThinPrep (Hologic, Bedford, MO Papanicolaou samples. Results: The positivity for the HPV E6/E7 messenger RNA (mRNA) assay was 94.4%, 95.2%, 82.4%, 46.5%, 25.0%, and 1.1% in squamous cell carcinomas, high-grade squamous intraepithelial lesions (HSILs), atypical squamous cells-cannot exclude HSIL, low-grade squamous intraepithelial lesions, atypical squamous cells of undetermined significance, and normal cytology samples, respectively. Five cervical intraepithelial neoplasia grade 2+ samples were not detected by the HPV E6/E7 mRNA assay, but they exhibited positive signals in the hTERT mRNA assay. Notably, the hTERT mRNA expression level was increased in high-grade cervical lesions but was very low in all 288 normal samples. Conclusions: These data suggest that the combination of HPV E6/E7 and hTERT mRNA expression levels could be used in a complementary manner in diagnosing high-grade cervical lesions and malignant tumors and might be useful as a predictive marker in monitoring low-grade cervical lesions.</P>

Evaluation of Commercial Complementary DNA Synthesis Kits for Detecting Human Papillomavirus

Kwangmin Yu,Sunyoung Park,Yunhee Chang,Dasom Hwang,Geehyuk Kim,Jungho Kim,Sunghyun Kim,김은중,Dongsup Lee 대한임상검사과학회 2019 대한임상검사과학회지(KJCLS) Vol.51 No.3

Cervical cancer is the fourth most common malignant neoplasm in women worldwide. Most cases of cervical cancer are caused by an infection by the human papillomavirus. Molecular diagnostic methods have emerged to detect the HPV for sensitivity, specificity, and objectivity. In particular, real-time PCR has been introduced to acquire a more sensitive target DNA or RNA. RNA extraction and complementary DNA synthesis are proceeded before performing real-time PCR targeting RNA. To identify an adequate and sensitive cDNA synthesis kit, this study evaluated the two commonly used kits for cDNA synthesis. The results show that the R2 and efficiency (%) of the two cDNA synthesis kits were similar in the cervical cancer cell lines. On the other hand, the Takara kit compared to Invitrogen kit showed P<0.001 in the 102 and 103 SiHa cell count. The Takara kit compared to the Invitrogen kit showed P<0.001 in the 101 and 102 HeLa cell count. Furthermore, 8, 4, 2, 1, and 0.5 ml of forty exfoliated cell samples were used to compare the cDNA synthesis kits. The Takara kit compared to the Invitrogen kit showed P<0.01 in 8, 4, and 1 ml and P<0.05 in 0.5 mL. The study was performed to identify the most appropriate cDNA synthesis kit and suggests that a cDNA synthesis kit could affect the real-time PCR results. Cervical cancer is the fourth most common malignant neoplasm in women worldwide. Most cases of cervical cancer are caused by an infection by the human papillomavirus. Molecular diagnostic methods have emerged to detect the HPV for sensitivity, specificity, and objectivity. In particular, real-time PCR has been introduced to acquire a more sensitive target DNA or RNA. RNA extraction and complementary DNA synthesis are proceeded before performing real-time PCR targeting RNA. To identify an adequate and sensitive cDNA synthesis kit, this study evaluated the two commonly used kits for cDNA synthesis. The results show that the R2 and efficiency (%) of the two cDNA synthesis kits were similar in the cervical cancer cell lines. On the other hand, the Takara kit compared to Invitrogen kit showed P<0.001 in the 102 and 103 SiHa cell count. The Takara kit compared to the Invitrogen kit showed P<0.001 in the 101 and 102 HeLa cell count. Furthermore, 8, 4, 2, 1, and 0.5 ml of forty exfoliated cell samples were used to compare the cDNA synthesis kits. The Takara kit compared to the Invitrogen kit showed P<0.01 in 8, 4, and 1 ml and P<0.05 in 0.5 mL. The study was performed to identify the most appropriate cDNA synthesis kit and suggests that a cDNA synthesis kit could affect the real-time PCR results.

스마트 TV의 UX 향상을 위한 UI 기술 동향

이기혁(Geehyuk Lee),김문철(Munchurl Kim),이우훈(Woohun Lee) 한국정보과학회 2011 정보과학회지 Vol.29 No.8

스마트 워치를 이용한 스마트 글래스 문자 입력 방법 연구

김선범(Sunbum Kim),이기혁(Geehyuk Lee) 한국HCI학회 2018 한국HCI학회 학술대회 Vol.2018 No.1

스마트 글래스는 빠른 정보 접근이 가능하기 때문에 유망한 웨어러블 디바이스 중 하나이다. 하지만 현재 스마트 글래스에서 제공하는 문자 입력 방법은 제한된 경우에만 사용 가능하거나 추가적인 디바이스를 지니고 다녀야 하는 번거로움이 있다. 우리는 효과적인 스마트 글래스 문자 입력 방법으로 웨어러블 디바이스인 스마트 워치를 이용한 문자 입력 방법을 고려했다. 우리는 이러한 컨셉을 제시한 이전 연구인 HoldBoard 를 참고하여 상용 스마트 워치를 그대로 이용하여 스마트 글래스에서 문자 입력을 할 수 있는 방법인 WatchBoard 를 구현하였다. 사용자 실험 결과, WatchBoard 는 추가 센서를 요구하는 HoldBoard 와 달리 상용 스마트 워치를 그대로 활용하면서 Holadboard 와 비슷한 문자 입력 속도와 더 낮은 오타율을 보였다. 우리는 WatchBoard 가 효과적인 스마트 글래스 문자 입력 방법으로 사용될 수 있을 것이라고 생각한다.

Human Papillomavirus Distribution among Women in Western Shandong Province, East China using Reverse Blot Hybridization Assay

Dongsup Lee,Geehyuk Kim,Sunghyun Kim,Sunyoung Park,Hye-young Wang,Sangjung Park,Lin Han,Ren Yubo,Yingxue Li,Kwang Hwa Park,Hyeyoung Lee 대한의생명과학회 2015 Biomedical Science Letters Vol.21 No.2

Cervical cancer is the third most common cancer in women worldwide and there is a significant association between human papillomavirus (HPV) infection and cervical cancer. Certain HPV groups, labeled high-risk (HR) HPV groups, are strongly associated with malignancies of the human cervix. HPV prevalence and genotype distribution were analyzed using the REBA HPV-ID<SUP>®</SUP> (YD Diagnostics, Yongin, Korea) assay based on the reverse blot hybridization assay (REBA) with a total of 324 liquid-based cytology samples from women in Western Shandong Province, East China and results were compared with cytological diagnosis. Most of the HPV genotypes that were detected in high-grade cervical lesions were HR-HPV genotypes such as HPV 16, 18, 33, 53, and 58. The prevalence of these HR-HPV genotypes increased in high-grade cervical lesions. However, from low- to high-grade cervical lesions, the ability to detect LR-HPV genotypes decreased. Additionally, in general, the single HPV genotype infection rate increases in proportion to the severity of the lesion. The study findings suggest that a currently available preventive vaccine against HPV 16 and 18 may have limited effectiveness for prevention of all HPV infection in this province. Finally, based on these findings, these data could guide national or regional vaccination programs in the Western Shandong Province of East China to substantially reduce the burden of cervical lesions.