http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
New Hairpin RNAi Vector with Brassica rapa ssp. pekinensis Intron for Gene Silencing in Plants
Lee, Gi-Ho,Lee, Gang-Seob,Park, Young-Doo Korean Society of Horticultural Science 2017 원예과학기술지 Vol.35 No.3
Homology-specific transcriptional and post-transcriptional silencing, an intrinsic mechanism of gene regulation in most eukaryotes, can be induced by anti-sense, co-suppression, or hairpin-based double-stranded RNA. Hairpin-based RNA interference (RNAi) has been applied to analyze gene function and genetically modify crops. However, RNAi vector construction usually requires high-cost cloning steps and large amounts of time, or involves methods that are protected by intellectual property rights. We describe a more effective method for generating intron-spliced RNAi constructs. To produce intron-spliced hairpin RNA, an RNAi cassette was ligated with the first intron and splicing sequences of the Brassica rapa ssp. pekinensis histone deacetylase 1 gene. This method requires a single ligation of the PCR-amplified target gene to SpeI-NcoI and SacI-BglII enzyme sites to create a gene-specific silencing construct. We named the resulting binary vector system pKHi and verified its functionality by constructing a vector to silence DIHYDROFLAVONOL 4-REDUCTASE (DFR), transforming it into tobacco plants, and confirming DFR gene-silencing via PCR, RT-qPCR, and analysis of the accumulation of small interfering RNAs. Reduction of anthocyanin biosynthesis was also confirmed by analyzing flower color of the transgenic tobacco plants. This study demonstrates that small interfering RNAs generated through the pKHi vector system can efficiently silence target genes and could be used in developing genetically modified crops.
LIMIT WEAK SHADOWABLE TRANSITIVE SETS OF C<sup>1</sup>-GENERIC DIFFEOMORPHISMS
Lee, Man-Seob,Lu, Gang Korean Mathematical Society 2012 대한수학회논문집 Vol.27 No.3
In this paper, we prove that locally maximal transitive set of a $C^1$-generic diffeomorphism is hyperbolic if and only if it is limit weak shadowable.
전자 현미경상 Epinephrine 과립이 관찰된 Zuckerkandl 기관에 발생한 Norepinephrine 분비성 갈색세포종 1예
이원석,김의숙,이강룡,이정아,장지정,선우인철,김대하,이광섭,백영주 대한내분비학회 1996 Endocrinology and metabolism Vol.11 No.2
Extraadrenal pheochromocytomas developed from the paraganglia other than adrenal medulla are uncommon tumors. Such lesions have been reported that as much as 10% of retroperitoneal paragliomas arise outside the adrenal gland. But their incidence and prognosis are changing partly as a result of progression in developmental mechanism, diagnostic tools and concepts including ultrastructure, immunohistochemistry. The ultrastructure of chromaffine neoplasm from adrenal and extraadrenal tissues has been studied with chrornaffine granule specificity and type of catecholamine content. With rare exceptions, extraadrenal chrornaffine tumor mainly has high concentration of norepinephrine which is suggested as dict relationship to numoruos electrone dense granular bodies in the cell cytoplasrm. High or intermediate level of epinephrine in tissue is associated with cytoplasmic granular bodies of much less density. Recently we experienced the exception, a case of norepinephrine secreting pheochrom-ocytoma in the organ of Zuckerkandl with epinephrine granules in cytoplasm and this discrepancy probably due to the plasticity - the differences in developmental process and local factors between adrenal medulla and extraadrenal tissues(J Kor Soc Endocrinol 11:233 ~239, 1996).
Development of traditional wine using biomass from alcoholic fermentation by Phellinus sp. mycelium
Hak-Seob Lim,Gang-Young Lee,Eun-Jung Jung,Won-Su Lee,Young-Kwang Ju,Min-Jeong Seo,Ji-Eun Kim,Tae-Yeon Kim,Hey-Rin Lee,Kyung Tae Chung,Byung Tae Choi,Young Hee Kim,Yong Kee Jeong 한국생명과학회 2006 한국생명과학회 심포지움 Vol.47 No.-
학술연구 : 기업벤처캐피탈이 IPO 성과에 미치는 효과 분석
이기환 ( Lee Gi Hwan ),김강혁 ( Kim Gang Hyeog ),윤병섭 ( Yun Byeong Seob ) 한국중소기업학회 2003 기업가정신과 벤처연구 Vol.6 No.1
This study explores the short-term performance of initial public offerings of corporate venture capital-backed firms. The aftermarket performance is empirically examined by utilizing 97 firms listed on the KOSDAQ market from January 2000 to February 2002. We analyse the impact of corporate venture capital through exhibiting the difference in performance, business history, holding ratio between CVC-backed and non-CVC-backed firms. In order to conduct our research, we classify the sample into three groups : corporate venture capital directly-backed firms, corporate venture capital indirectly-backed firms and non venture capital-backed firms. Using ANOVA, the difference on initial returns, business history between three groups is explored. And using t-test method, the change of corporate venture capital`s holding between two CVC-backed groups after listing on KOSDAQ is analyzed. The major results of this thesis are as follows. First, we find that as the time after the listing day passes, corporate venture capital directly-backed firmsl` excess return shows higher than non-venture capital-backed firmsl` abnormal return. However, corporate venture capital indirectly-backed firmsl` return exhibits lower than non-venture capital-backed firmsl` return. In KOSDAQ`s IPOs market, corporate venture capitals could not reduce statistically the information asymmetry between public investors and issuing firms. Secondly, our empirical analysis shows that the business history of corporate venture capital indirectly-backed firms are shorter than that of non-venture capital-backed firms. But it fails to find that the business history of corporate venture capital directly-backed firms would be longer than that of corporate venture capital in directly-backed firms. This is inconsistent with the result of Gompers(1996) which found the grandstanding in the US IPOs market. Thirdly, this study also investigates whether there is the difference in the changes of corporate venture capitalsl holdings between corporate venture capital directly-backed firms and corporate venture capitalist indirectly-backed firms before and after going public. The holdings of corporate venture capital indirectly-backed firms are decreased more than corporate venture capital directly-backed firms.
진균구로 오인된 낭포내 응혈을 보인 23세 여자에서의 선천성 낭포성 유선종 폐기형 1례
이강룡 ( Gang Ryong Lee ),이군순 ( Kun Sun Lee ),주인규 ( In Kyu Joo ),박준옥 ( June Ock Park ),최승준 ( Seung Jun Choi ),이원석 ( Won Seok Lee ),김의숙 ( Eui Sook Kim ),이규현,김대하 ( Dae Ha Kim ),이광섭 ( Gwang Seob Lee ),한영숙 ( 대한결핵 및 호흡기학회 1999 Tuberculosis and Respiratory Diseases Vol.46 No.1
Up-dating of new dCAPS markers for mapping yield-related traits using MGRIL
Ye-Ji Lee,Hyun-Ju Lee,In-Seon Jeong,Seon-Hwa Bae,Hyeon-So Ji,Gang-Seob Lee,Ung-Han Yoon,Jang-Ho Hahn,Tae-Ho Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
The next-generation sequencing(NGS) technology is being used for more effective genetic mapping. In previous study, we obtained 60x coverage of sequence from Milyang23 and Gihobyeo on average comparing with Nipponbare reference genome. Also, we developed new derived cleaved amplified polymorphic sequence(dCAPS) markers based on the single nucleotide polymorphisms(SNPs) in coding region sequence(CDS) between these varieties. Totally, 1,726,798 SNPs between Milyang23 and Gihobyeo were detected. Among them, 146 SNP were selected for making dCAPS markers and located on genetic map with previously reported 219 PCR-based DNA markers. The map was applied to the detection of quantitative trait loci(QTLs) for stem internode diameters, culm length and panicle length within MGRIL population, and six QTLs with relatively high LOD score were found at three chromosomes; culm length and stem diameter including the first internode diameter, third and fourth internode diameter. This study showed that the NGS allowed the rapid discovery of a large number of SNPs for dCAPS marker. So, we tried to find out more single nucleotide polymorphisms(SNPs) which were located on the whole genome sequence, such as un-translated region(UTR), intron, Inter-region and coding region sequence(CDS) between Milyang23 and Gihobyeo varieties. And we collected phenotypic information about culm length, panicle length, four stem internode diameters and panicle number in rice MGRIL population for QTLs. Furthermore, results of QTL analysis described above will shows relevance of molecular markers in mapping genes for useful breeding.
Mapping of QTLs for stem diameter in MGRIL using CAPS markers
Hyun-Ju Lee,In-Seon Jeong,Hyeonso Ji,Gang-Seob Lee,Ung-Han Yoon,Jang-Ho Hahn,Tae-Ho Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
The next-generation sequencing (NGS) technology is being used for more effective genetic mapping and genome analysis. In this study, we performed whole-genome sequencing on the genomic DNA of Milyang23 and Gihobyeo using NGS and developed new cleaved amplified polymorphic sequence (CAPS) markers based on the single nucleotide polymorphisms (SNPs) in coding sequence between these varieties. Approximately, sequences of 60x coverage of the Nipponbare reference genome on average were obtained following Illumina sequencing. Totally, 1,726,798 SNPs between Milyang23 and Gihobyeo were detected. Among them, 149 SNP were selected for CAPS markers and located on genetic map with previously reported 219 PCR-based DNA markers. This map was applied to the detection of quantitative trait loci (QTLs) for stem internode diameters, culm length and panicle length in rice with MGRIL population. Newly 6 QTLs were detected for culm length (CL) and stem diameter (ID) traits including the first internode diameter (I1D), third internode diameter (I3D), and fourth internode diameter (I4D). Among those QTLs, qI1D5 and qCL5 had relatively higher LOD score and explained 8.99% and 4.24% of total variation. This study showed that the NGS allowed the rapid discovery of a large number of SNPs for CAPS marker. Only very small portion of SNPs through re-sequencing were used in this study. Furthermore, the results of QTL analysis described above shows relevance of molecular markers in mapping genes for useful traits.