혈액투석 환자에게서 발생한 재생불량성 빈혈 1예

정지용,윤나라,강대웅,오종찬,장재현,선길홍,박경희,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

Aplastic anemia is a disease characterized by pancytopenia in the peripheral blood and hypocellular bone marrow with the fatty infiltration. The causes of the acquired aplastic anemia were usually related to drugs and immune-related diseases. A 34-years old man was admitted due to general weakness. He has been going hemodialysis for 2 years because of end stage renal disease (ESRD) due to chronic glomerulonephritis. At admission, laboratory tests showed hemoglobin 6.2 g/dL, hematocrit 18.0 %, WBC 5,710mm (neutrophil 71.8%, lymphocyte 19.1%, monocyte 5.9%), reticulocyte 1.9%, platelet 93,000/mm, Fe 12.8 ug/dL, TIBC 204 ug/dL, ferritin 941.47 ng /ml, haptoglobin 0.72 g/L, vitamin B12 508.17 pmol/L, folate 24 ng/mL, total protein 6.54 g/dL, albumin 3.76 g/dL, Alk. pohsphatase 79 IU/L, AST 30.0 IU/L, ALT 39.7 IU/L, total bilirubin 0.57 mg/dl, BUN 79.4 mg/dl and creatinine 10.18 mg/dl. Peripheral blood smear showed the normocytic normochromic anemia with anisocytosis. Anemia was diagnosed but it was irresponsive to the recombinant human erythropoietin (EPO) and blood transfusion. In the bone marrow aspiration smear were there the hypoplastic myeloid and erythroid precursors. The bone marrow biopsy section showed the hypoplasia of all components (10-20%) and the fatty infiltration. We have experienced one case of the idiopathic aplastic anemia in a patient going through hemodialysis and we just report it with documentary records. 저자들은 혈액 투석 중인 환자에서 약물, 면역계 이상등의 원인을 발견할 수 없었음에도 재생 불량성 빈혈이 발생한 1예를 경험하여 문헌 고찰과 함께 보고하는 바이다.

공침법에 의한 BiDy₂Fe_(5)O_(12) 결정형 나노입자 분말의 제조 및 응용 연구

홍영대,조정환,김태엽,전성욱,홍영명 고신대학교 자연과학연구소 2007 고신대학교 자연과학연구소 논문집 Vol.14 No.-

금속산화물들은 각종 산업재료로서 유용하며 더욱이 그것들의 결정형 나노입자를 경제적으로 제조하는 기술은 21세기 첨단산업에 매우 중요하다. 저자들은 前報에서 화학합성법에 의한 각종 금속산화물의 나노입자를 제조하는 표준공정법을 연구하여 보고하였다. 본 연구에서는 그 표준공정법을 이용하여, 가넷의 일종인 BiDy_(2)Fe_(5)O_(12) 결정 나노입자 분말을 제조하고 그것의 자성체로의 응용을 위해 제품특성을 조사하었다. 가네트 제조과정에 필요한 침전물은 제품설계에 의거 몰비로 계산된 Bi, Dy, Fe의 질산염들의 혼합수용액에 침전제로서 pH NH_(3)-NH_(4)CI 완충용액 일정량을 가하여 얻어졌다. 이렇게 제조된 침전은 열처리과정을 거처 BiDy_(2)Fe_(5)O_(12) 결정형 나노입자 분말로 만들어졌다. 얻어진 제품의 입자크기, 조성, 결정구조 및 광자기성 등 특성시험이 SEM, ICP, XRD, 그리고 VSM 등을 사용하여 실시되었다. 각종 분석법을 사용하여 제품의 특성을 조사한 결과, 최초에 설계한 대로의 자성체가 나노 크기의 분말로 얻어졌으며 이것을 통하여 전보에서 확립한 표준공정법의 유효성도 입증되었다. Metallic oxides are useful as various industrial materials. Futhermore, the cost-effective techniques of fabricating the crystalline nanopartides of metallic oxides are very important for the high-tech industries of the 21 century, In the previous study, the authors reported the standard process for the preparation of crystalline nanoparticles of metallic oxides by chemical synthesis, In this study, BiDyzFesOiz nanoparticles were prepared by using the reported standard process and the characteristics of the product were investigated for applicability as magnetic substance. The precipitation needed for manufacturing process of garnet was made by adding the NH_(3)-NH_(4)CI buffer solution of pH 10 to the mixed solution of bismuth (III) nitrate, dysprosium(III) nitrate and iron(III) nitrate. The amount of each nitrate was calculated by the designed mole ratios. The nano-size crystalline powders of BiDy_(2)Fe_(5)O_(12) were earned by heating the precipitation at 800℃ for 4 hours in air. The characterizations, such as particle size, composition, crystal structure and magneto-optical property of garnet were tested by SEM, ICP, XRD, and VSM analyses. The results of the analyses showed the following two facts. First, a good magnetic substance of BiDy_(2)Fe_(5)O_(12) was earned as crystalline nano-size powders according to the original design. Next, the established standard process for the fabrication of METALLIC oxides nanonartieles was approved as a very effective method.

超微量 亞鉛의 螢光光度定量法

洪永大,李正禹 고신대학교 자연과학연구소 1997 고신대학교 자연과학연구소 논문집 Vol.7 No.-

超微量의 Zn을 定量하기 위하여 2-(o-hydroxyphenyl)benzoxazole (HPB)을 利用하는 高感度의 螢光分析法이 確立되었다. 0.65㎍ 以下의 Zn(Ⅱ) 이온을 含有하는 試料溶液에 이것에 比하여 一定過量의 HPB 溶液을 加하여 約 pH 9.4에서 反應시키면 螢光이 없고 安定度가 큰 Zn(HPB)_2 의 킬레이트 化合物을 만들어 定量的으로 沈澱한다. 그런 다음, 溶液 中에 남아 있는 HPB의 螢光强度를 測定함으로써 Zn(Ⅱ) 이온의 濃度를 間接 測定하였다. 本 硏究에서는 nmol level의 超微量 濃度에서 Zn(Ⅱ) 이온과 HPB의 反應이 化學量論的으로 일어 나는데 必要한 最適條件들을 綿密히 檢討한 結果, 0.5-10 nmol의 Zn을 感度좋게 定量할 수 있었다. 本 定量法을 使用하여 5 nmol의 Zn을 反復 測定하여 본 結果, 相對標準偏差 2.1%의 精密度로서 測定할 수 있었다. A spectrofluorometric method for the ultra microdetermination of zinc was established. An excess amount of 2- (o-hydroxt'phenyl)benzoxazole(HPB) is added to precipitate Zn(HPB)₂ with zinc ion. Zinc ion forms a chelate with HPB and quenches the fluorescence of HPB. The amount of zinc is determined by measuring the fluorescence of the remaining HPB. The optimum conditions for these reactions were examined at a very low concentration range of the reactants. By the proposed method, 0.5-10 nmol zinc content was determined with a accuracy of 2.1% relative standard deviation at 5 nmol Zn level.

방향족 화합물 분해균주의 분리 및 동정

정준영,김교창,김도영,도대홍 충북대학교 농업과학기술연구소 1995 農業科學硏究 Vol.12 No.2

Derivation of reasonable design rainfall was attempted by comparative analysis of design rainfall derived by frequency analysis of Type I extremal distribution and probability plotting technique for annual maximum 1-day, 1-hour and 10-minute rainfalls observed at Cheong Ju. The results were analyzed and summarized as follows : 1. Basic statistics were calculated for the analysis of Type I extremal distribution with annual maximum 1-day, 1-hour and 10-minute rainfalls at Cheong Ju. 2. Necessary parameters were derived by frequency analysis of Type I extremal distribution for annual maximum 1-day, 1-hour and 10-minute rainfalls at Cheong Ju. 3. Design annual maximum 1-day, 1-hour and 10-minute rainfalls in mm were derived by frequency analysis of Type I extremal distribution and probability plotting technique respectively at Cheong Ju. 4. Design annual maximum 1-hour and 10-minute rainfalls were derived by frequency analysis of Type I extremal distribution and probability plotting technique are shown to be much closer to the observed data but design annual maximum 1-day rainfall is not to be closer to the observed data. 5. Design annual maximum rainfalls following the duration derived by Type I extremal distribution are seemed to be more reasonable than those of probability plotting technique owing to the difference of individual eyefitting.

Ethylenediaminetetraacetic acid(EDTA)항응고제에 의한 Mean Corpuscular Volume(MCV)변화

홍승민,박영진,최하영,임진,정옥연,이호준,이호영,장숙진,문대수 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.2

It is essential that we put the anticoagulant into the blood specimen of the CBC test. However, the value of some parameters in the CBC test can be changed whenever using some anticoagulants. EDTA anticoagulant is mostly used these days. There are 3 kinds of EDTA, that is K_(2-), K_(3-), and Na_(2-) EDTA. The author studies how many effects each the EDTA gets into the true value of blood specimen for MCV of RBCs. Especially we compared the MCV effect of different concentrations of 3 EDTA anticoagulants on the bias with time and instrument. Some results are gotten. In our results, K_(3-)EDTA anticoagulant shows more satisfactory MCV value than K_(2) or Na_(2-)EDTA. On using concentration of EDTA, 1.5㎎ EDTA/㎖ of blood show more satisfactory MCV value than 7.5㎎ EDTA/㎖ of blood. However, the choice of anticoagulant may be dependant on the different instruments.

악하선에서 분리된 NGF에 의해 유도되는 비만세포 활성화에 미치는 상백피의 억제효과

정홍주,백병주,김대범 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.2

Cortex Mori(Norus alba L), the root bark of mulberry tree has been used as an antiphlogistic, diuretic, and expectorant in herbal medicine. Previous studies have demonstrated that Cortex Mori had inhibitory effects on the compound 48/80-induced degranulation and histamine release from rat peritoneal mast cells(RPMC). The purpose of this study is to determine whether Cortex Mori(CM) could inhibit the nerve growth factor(NGF)-induced activation of RPMC in vivo and in vitro. In vitro, various concentrations of NGF(0.1, 1, 10 and 100㎍/ml) were added into the RPMC suspension(??cells/ml) pretreated with lyso-PS at 37℃ for 10minutes. Cortex Mori pretreatment was performed before or after lyso-PS treatment at 37℃ for 10minutes. Histamine in the supernatants and calcium uptake of RPMC were measured by radioisotope enzymatic histamine assay and radioisotope calcium uptake method. In vivo experiment, to estimate the effect of Cortex Mori on NGF-induced cutaneous reaction, various doses of NGF with or without Co rtex Mori on NGF-induced cutaneous reaction, various doses of NGF with or without Cortex Mori were injected i.d. int the shaved flank of Sprague-Dawley rats, and the blue cutaneous staining induced by Evans’blue injection at the NGF alone Cortex Mori plus NGF injection sites were observed. Results were 1) the NGF-induced degranulation and histamine release of RPMC pretreated with Cortex Mori were significantly inhibited, compared to those of control without Cortex Mori pretreatment,2) Cortex Mori inhibited the NGF-induced cutaneous reaction remarkably. From the above results, it is suggested that Cortex Mori contains some substances with an activity to inhibit the NGF-induced activation of mast cell.

전하 제어법을 이용한 압전 액추에이터의 이력저감

정순종,이대수,송재성,홍원표,강은구,최원종 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

This paper presents a method to reduce hysteresis in multilayer ceramic actuator by connecting the actuator with a capacitor in a series circuit. The change in hysteresis with respect to the capacitor was examined. 0.2Pb(Mg_(1/3)Nb_(2/3))O₃-0.8Pb(Zr_(0.475)Ti_(0.525)) O₃ ceramic material was used as a piezoelectric material for the actuator. Displacement of the actuator was measured in a capacitive gap sensor measuring system. In case of inserting a capacitor in a total circuit, hysteresis became dramatically decreased, and then finally the hysteresis value can be reduced below 0.2%. It was found in this present study that reducing the hysteresis in the actuator is dependent upon the characteristics of the capacitor in total circuit and also operating frequency

Ni1-xCdxFeAlO₄계의 Mossbauer 스펙트럼 연구

홍성락,고정대,백승도 濟州大學校 基礎科學硏究所 1998 基礎科學硏究 Vol.11 No.2

Ni1-xCdxFeAlO₄계의 Mossbauer스펙트럼은 x가 0.2이하인 경우에는 초상자성 이완현상을, x가 0.3~0.5인 경우에는 준강자성에 의한 sextet를 x가 0.7, 0.8인 경우에는 강자성 이완현상, x가 0.8이상인 경우에는 상자성에 의한 doublet가 나타난다. x가 0.2이하인 시료에서 치환된 Cd²이온은 주로 A-site의 Al³이온을 B-site로 이동시키고, 계속적인 Cd²이온의 증가에 따라 A-site의 Fe³+ 이온도 B-site로 이동하게 된다. Ni1-xCdxFeAlO₄계의 초상자성 이완 현상은 A-site의 Al³이온에 의한 것으로 강자성 이완현상은 치환된 Cd²+이온에 의한 것으로 설명할 수 있고, Yafet-Kittel자기구조에 따라 Ni1-xCdxFeAlO₄계의 자기적 성질을 해석할 수 있다. The Mossbauer spectra of the Ni1-xCdxFeAlO₄system were investigated with the Cd content X at room temperature. The spectra of the samples exhibit various patterns as follows 1) superparmagnetic relaxation for 0≤x≤0.2, 2)ferrimagnetic sextet for 0.3≤x≤0.5, 3) ferromagnetic relaxation for x=0.6, 0.7, 4)paramagnetic doublet for 0.8≤x≤1, with the Cd content x. In the samples with X values from O to 0.2, the substituted Cd²+ ions transfer the Al³+ ions from A-site to B-site mainly. The superparamagnetic relaxation effect and the ferromagnetic relaxation effect are derived from the Al³,Cd²respectively. The magnetic structrue of the Ni1-xCdxFeAlO₄system was explained by the Yafet-Kittel model.

Mossbauer 분광법을 이용한 제주 화산암의 미시적 연구와 그 응용

홍성락,고정대,김순희 濟州大學校 基礎科學硏究所 1994 基礎科學硏究 Vol.7 No.1

제주도 전 지역의 특정위치를 선정하여 그 위치에 따라 지하 60m, 61m, 87m, 99.5m, 220m까지 깊이별로 화산암 시료를 채취하여, 이 시료에 대한 SIMS, XPS와 Mossbauer spectroscopy의 실험결과를 보고한다. UHV chamber에서 분리 이온화에 의해 생겨 난 parent 이온들로부터 cracking pattern들에 대해 연구하였고, 그 parent 이온들은 Si??, Al??, Ca??, Mg??, Fe??, Fe??, Na?, k?등으로 나타났다. 이 시료들에 대한 XPS 실험에서 wide scan analysis를 통하여 시료 표면에서 Al, Si, Ca, O, Fe, Na 등의 원소가 존재하고 있음을 알 수 있었으며, narrow scan analysis를 통하여 이들 대부분이 single compound state를 나타내고 있음을 발견할 수 있었다. 화산암에 대한 Mossbauer spectrum으로부터 olivine, pyroxene, ilmenite에 의한 세쌍의 quadrupole splitting을 얻었으며, 각 광물에 함유된 Fe는 약간의 Fe??를 제외하고는 거의 모두 Fe??상태임을 확인하였다. We are reporting the results of measurements of the SIMS, XPS, and ??Fe Mossbauer spectrum for powder samples of volcanic rock which has been distributed throughout different areas on Cheju island, each in different depth beginning at 60m then 61m than 61m, 87m, 99.5m, and 220m. We have studied the cracking patterns from parent ions which are created by dissociative ionization in the UHV chamber. The parent ions were Si??, Al??, Ca??, Mg??, Fe??, Fe??, Na??, and k??. In the XPS results for these samples, we find the existence of elements Al, Si, Ca, O, Fe, Na on their surfaces through the wide scan analysis. Also, the single compound state has appeared in a greater percentage of the them through the narrow scan analysis. The Mossbauer spectrum consists of well defined quadrupole doublets having a dominant 2+ charge state with a small percent having a 3+ charge state. These indicate the presense of olivine, pyroxene, and ilmenite.

Ethylenediaminetetraacetic acid (EDTA)항응고제에 의한 Mean Corpusculaar Volume(MCV)변화

홍승민,박영진,최하여,임진,정옥연,이호준,이호영,장숙진,문대수 조선의대 1996 The Medical Journal of Chosun University Vol.21 No.2