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MSK1 regulates RANKL-induced NFATc1 expression through CREB and c-Fos
Jeongim Ha,Jung Hye Hwang,Seul Gi Kwon,Da Hye Park,Tae Wan Kim,Deok Gyeong Kang,Kyung Hee Kang,Chul Wook Kim,Il-Suk Kim 충북대학교 동물의학연구소 2015 Journal of Biomedical and Translational Research Vol.16 No.2
Osteoclasts originated from hematopoietic stem cells are multi-nucleated cells that can resorb the bone matrix. Receptor activator of nuclear factor kappa-B (RANK)/RANK ligand (RANKL) signaling pathway is crucial for the differentiation and activation of osteoclasts. In this study, we investigated for the first time whether or not RANKL induced mitogen- and stress-activated kinase 1 (MSK1) phosphorylation at Ser 376. Activation of MSK1 was detected as soon as 5 min after RANKL stimulation and sparsely detected at 30 min after stimulation. RANKL-induced MSK1 phosphorylation occurred in a dose-dependent manner. MSK1 is known as a downstream signaling molecule of cAMP-dependent protein kinase (PKA). Treatment with the PKA inhibitor H89 significantly suppressed c-Fos and nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) induction upon RANKL stimulation. In addition, cAMP response element-binding protein (CREB) phosphorylation was extremely inhibited by H89 treatment. Mitogen-activated protein kinases (MAPKs) have been investigated for induction of MSK1 phosphorylation. Specific signaling pathway inhibitors for p38 and extracellular signal-regulated kinases (ERKs) significantly blocked RANKL-induced MSK1 activation. Finally, as a downstream effector of the p38-MSK1 pathway, c-Fos transcriptional activity was determined. RANKL-mediated elevation of c-Fos transcriptional activity was significantly suppressed by p38 inhibitor. Moreover, a dominant negative form of CREB suppressed activation of NFATc1. In conclusion, RANKL-stimulated MSK1 phosphorylation could play a role in induction of NFATc1 through CREB and c-Fos activation as a downstream molecule of p38, ERK MAPKs, and PKA. Our results support basic information for the development of osteoclast specific inhibitors.
Molecular characterization of porcine <i>PGM1</i> gene associated with meat quality traits
Hwang, Jung Hye,Kwon, Seul Gi,Park, Da Hye,Kim, Tae Wan,Kang, Deok Gyeong,Ha, Jeongim,Kim, Sam Woong,Kim, Chul Wook Canadian Science Publishing 2015 Canadian journal of animal science Vol.95 No.1
<P> Hwang, J. H., Kwon, S. G., Park, D. H., Kim, T. W., Kang, D. G., Ha, J., Kim, S. W. and Kim, C. W. 2015. Molecular characterization of porcine PGM1 gene associated with meat quality traits. Can. J. Anim. Sci. 95: 31-36. The PGM1 gene from four porcine breeds (Berkshire, Duroc, Landrace, and Yorkshire) is highly expressed in liver tissue at the transcriptional level. Single nucleotide polymorphisms (SNPs) of PGM1 were examined to analyze association with increased expression of PGM1 gene in the Berkshire liver. A Leu525 synonymous SNP of Chr6:137174682A@@>@@G (c.1575A@@>@@G) was identified and showed significant (P@@<@@0.05) differences to backfat thickness, drip loss, protein content, fat content, Warner-Bratzler shear force, and post-mortem pH24h. Therefore, it is concluded that PGM1 synonymous SNP is an important factor regulating meat quality. </P>
Hwang, Jung Hye,An, Sang Mi,Kwon, Seul Gi,Park, Da Hye,Kim, Tae Wan,Kang, Deok Gyung,Yu, Go Eun,Kim, Il-Suk,Park, Hwa Chun,Ha, Jeongim,Kim, Chul Wook Informa UK (TaylorFrancis) 2017 Animal biotechnology Vol.28 No.4
<P>Postmortem pH is a main factor influencing the meat quality in pigs. This study investigated the association of postmortem pH with single-nucleotide polymorphisms (SNPs) in the fourth member of the short-chain dehydrogenase/reductase family (DHRS4), the first member of serpin peptidase inhibitor, clade G (complement inhibitor) (SERPING1), and the apolipoprotein R precursor (APOR) genes in Berkshire pigs. The study included 437 pigs, and genotyping was conducted using the GoldenGate Assay (Illumina, San Diego, CA, USA). DHRS4, SERPING1, and APOR polymorphisms were significantly associated with pH(45) or pH(24) (p<0.05). SERPING1 was also statistically significantly associated with water holding capacity (p<0.05), which is closely associated with postmortem pH. These results suggest that SNPs in the DHRS4, SERPING1, and APOR genes have potential for use as genetic markers for the meat quality in pigs.</P>
Kwon, Seul Gi,Hwang, Jung Hye,Park, Da Hye,Kim, Tae Wan,Kang, Deok Gyeong,Kang, Kyung Hee,Kim, Il-Suk,Ha, Jeongim,Kim, Chul Wook,Plaizier, J. Canadian Science Publishing 2016 Canadian journal of animal science Vol.96 No.1
<P> Corticosteroid-binding globulin (CBG) is a plasma transport protein that has glucocorticoid-binding activity. In the present study, we identified CBG gene expression in several tissues of four pig breeds: Berkshire, Duroc, Landrace, and Yorkshire. Expression of CBG mRNA was detected in the liver of all four breeds, and was the highest in Berkshire pigs. We also found single nucleotide polymorphisms (SNPs) in the CBG gene from Berkshire pigs, including SNP c.919G@@>@@A, which corresponds to 307G@@>@@R. We analyzed the relationships between this CBG variant and various meat-quality traits. The SNP was significantly associated with backfat thickness, post-mortem pH24h, meat color [CIE a* (redness), CIE b* (yellowness)], water-holding capacity, fat content, moisture content, protein content, cooking loss, and shear force. However, the effects differed by gender: the values were significantly associated with almost all traits in gilts, whereas only cooking loss and shear force were shown significantly in barrows. The variant G allele was associated with decreases in backfat thickness, post-mortem pH24h, CIE a*, fat content, and cooking loss, but with increases in CIE b*, water-holding capacity, moisture content, protein content, and shear force. Because the general correlation between meat-quality traits were not validated in the present study, we suggest that certain SNP might be used in the restrictive application to distinguish meat-quality traits. </P>
Kim, Da-Seul,Hwang, Nong-Moon IOP 2018 Journal of Physics. D, Applied Physics Vol.51 No.46
<P>Nonclassical crystallization is the process in which crystals grow by deposition of nanoparticles rather than by the layer-wise deposition of atoms/ions/molecules on a crystal surface. A spontaneous generation of charged nanoparticles (CNPs) in the gas phase has been experimentally confirmed during many chemical vapor deposition (CVD) processes. The fact that crystals grown by CNPs have smooth surfaces without any void indicates that CNPs have a liquid-like or superplastic property. Based on this understanding of nonclassical crystallization in CVD processes, the synthesis of nanoparticles, thin films and nanowires can be approached systematically. Since electrical field, drag effect, size and the number of charges per particle affect the behavior of CNPs, electric bias, gas velocity, the size of the nanoparticles and the amount of charge can be new processing parameters. The size distribution of CNPs depends on the process conditions.</P> <P>The nanoparticles can be attracted or repelled by applying an electric bias to a substrate. If the nanoparticles are made to land on the substrate in a suitable number density, they can be used as quantum dots. In addition, nanopowders, nanowires, and nanostructures can be synthesized by CNPs with or without metallic catalysts on substrates. Herein, we will review recent results of the synthesis of nanostructures such as nanoparticles, thin films, and nanowires in the CVD process. We will also discuss the role of charge in these syntheses.</P>
Synthesis of hollow carbon nanostructures as a non-precious catalyst for oxygen reduction reaction
Lee, Seul,Kwak, Da-Hee,Han, Sang-Beom,Hwang, Eui-Tak,Kim, Min-Cheol,Lee, Jin-Yeon,Lee, Young-Woo,Park, Kyung-Won Elsevier 2016 ELECTROCHIMICA ACTA Vol.191 No.-
<P><B>Abstract</B></P> <P>Carbon-based nanostructures as a non-precious catalyst for oxygen reduction reaction (ORR) exhibit excellent electrocatalytic activities due to high specific surface areas and favorable active sites supported by the doped transition metals and nitrogen species. We synthesize the metal-nitrogen doped carbon nanostructures (MPc-C, M=Fe,Co) by using a template method in the presence of metal phthalocyanine including FePc, CoPc, and FeCoPc as metal and nitrogen as well as carbon sources. The as-prepared carbon nanostructures show a high specific surface area, porous structure, and metal-nitrogen dopants, as observed by field-emission scanning electron microscopy (FE-SEM), field-emission transmission electron microscopy (FE-TEM), X-ray photo-electron spectroscopy (XPS), and nitrogen sorption measurement. Especially, among the doped carbon nanostructures, FeCoPc-C exhibits superior ORR electrocatalytic properties in an alkaline medium: an electron transfer number close to 4, high half-wave potential, improved electrochemical stability, and methanol tolerance.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We synthesized the metal-nitrogen doped carbon nanostructures using a facile method. </LI> <LI> The nanostructures consisted of a high specific surface area and porous structure. </LI> <LI> The nanostructures exhibited well-doped transition metal and nitrogen species. </LI> <LI> The nanostructures showed excellent electrocatalytic activities in ORR. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Visible-Light-Promoted Synthesis of Fluoroalkylated Oximes
Lee, Da Seul,Hwang, Ho Seong,Cho, Eun Jin Wiley (John WileySons) 2018 Chemistry, an Asian journal Vol.13 No.17
<P>A method has been developed for the synthesis of fluoroalkylated oximes, potential fluoroalkyl building-blocks for the synthesis of various organofluorine compounds, from easily available amino substrates and fluoroalkylated alkenes. tBuONO was utilized both as a diazotizing agent and as a NO radical source for the oxime synthesis in the process, and the use of a photocatalyst under visible-light irradiation increased the efficiency of the reaction. Various fluoroalkylated oximes were prepared by a tandem process of aryl radical addition to fluoroalkylated alkene and consecutive oxime generation process, albeit in moderate yields. This differentiated approach, transferring an aromatic system into an electron-deficient fluoroalkylated alkene, expands the scope of substrates where electron-poor aromatic systems could be utilized.</P>
Kim, Da-Mi,Kim, Si-Jin,Lee, Young-Woo,Kwak, Da-Hee,Park, Han-Chul,Kim, Min-Cheol,Hwang, Bo-Mi,Lee, Seul,Choi, Jong-Ho,Hong, Seongho,Park, Kyung-Won Elsevier 2015 ELECTROCHIMICA ACTA Vol.163 No.-
<P><B>Abstract</B></P> <P>Well-defined nanocomposite electrodes based on active materials and graphene have been known to hold improved lithium ion reaction properties such as the lithium ion insertion/desertion reaction, cycle life, and high rate performance. Here, we report two-dimensional nanocomposites consisting of WO<SUB>3</SUB> nanoplates and graphene nanosheets (GNS) using a hydrothermal method and heating process for use in high-performance lithium ion batteries. A compilation of the data from XRD, SEM, and TEM suggests that the nanocomposites consist of high crystalline WO<SUB>3</SUB> nanoplates and GNS. The capacity and high rate cycling performance of the nanocomposites as an anode for lithium ion batteries are evaluated using coin cells. The nanocomposite electrode with an optimized amount of GNS exhibits high reversible capacity, good capacity retention, and excellent high rate cycling performance.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>