돼지 卵子의 透明帶에 대한 單一클론抗體生産과 그 特性에 關한 硏究

金鐘培,劉永春,金昌圭,權五中,鄭盛元,鄭吉生 건국대학교 동물자원연구센터 1991 動物資源硏究誌 Vol.16 No.-

本 試驗은 單一클론抗體의 강한 特異性과 抗體性質의 不變性을 이용하여 發生學的 側面에서 哺乳動物 卵子의 透明帶의 機能과 構造를 이해하고, 또한 種特異的인 精子 受容體의 存在 및 生化學的 構造를 규명하기 위한 기초연구로서, 돼지 卵子의 透明帶를 免疫原으로 하여 BABL/c 생쥐로부터 單一클론抗體를 생산하고 그 특성을 구명하였던 바 다음과 같은 결과를 얻었다. 1) 3마리의 BABL/c 생쥐(YⅠ, YⅡ, ZI)에 돼지卵子의 透明帶를 免疫化하고, 複合抗體 生成을 확인한 후 생쥐의 脾臟細胞와 Myeloma(SP2/O-Ag14)를 polyethylene glycol를 融合을 실시한 결과 각각 25.8%, 54.5% 그리고 59.7%의 融合效率을 나타내었으며, ELISQ에 의해 陽性反應을 조사한 결과 각각 17.3%, 32.6% 그리고 6.2% 陽性反應 效率을 나타내었다. 2) YI에서 강한 陽性反應을 보인 6개의 well에 대한 cloning을 실시하고 抗體檢證을 행한 결과 20.8% ∼ 48.4%의 Cloning效率과 54.6% ∼ 82%의 陽性反應 效率을 나타내었다. 3) 강한 陽性反應을 나타낸 항체에 대해 sandwich ELISA法에 의해 isotype을 決定하였던바 2E93C(YⅠ), 3E83E7(YⅠ), 4E3(YⅡ)각각 IgG₂b, IgG₂a, IgM으로 확인되었다. 4) Isotype이 決定된 2E93C9(YⅠ), 3E84E7(YⅠ), 4E3(YⅡ)의 세포를 생쥐의 腹腔에 주사하여 얻은 腹水를 indirect ELISA法에 의해 titer를 決定한 결과 모두 1:400,000 이상의 높은 titer를 나타내었다. 5) 處理區로서 單一클론抗體의 腹水와 對照區로서 normal mouse serum이 각각 2%씩 함유된 배양액속에서 난자를 배양한 후 顯徵鏡下에서 관찰했을 때 對照區에서 배양된 난자의 표면은 정상적인 형태를 나타냈으나 處理區에서 배양된 卵子는 표면에 뚜렷한 沈澱層을 형성하였다. 6) 處理區와 對照區 卵子를 Rabbit anti-mouse IgG-FITC가 1% 함유된 배양액속에서 배양하고 洗滌한 후 螢光顯徵鏡下에서 관찰한 바 處理區의 卵子는 透明帶 주위에서 螢光이 나타났으나, 對照區에서는 나타나지 않았다. This study was carried out ot produce and characterize monoclonal antibodies against porcine zona pellucida, and undertaken as a basic study to develop immunocontraceptive vaccine and to investigate the function of zana pellucida in early fertilization process. The results obtained were summarized as follows: 1. Spleen cells of three BALB/C mice(YⅠ, YⅡ and ZI) which were immunized with porcine zona pelucida were fused with myeloma cells(SP2/O-Ag14) by polyethylene glycol. At the result of fusion, fusion efficiency was 25.8 , 54.5% and 59.7% and positive efficiency 17.3%, 32.6% and 6.2%, respectively. 2. Cloning efficiency was shown to be from 20.8% to 48.4% and positive efficiency of them were 54.6% to 82%. 3. Sub-isotypes of three strong positive antibodies, 2E93C(YⅠ), 3E83E7(YⅠ) and 4E3(YⅡ) were determined by sandwich ELISA method and shown to be as IgG2b, IgH2a and IgM, respectively. 4. The titers of three ascitic fluids containing antibodies, 2E93C9(YⅠ), 3E84E7(YⅠ) and 4E3(YⅡ) were determined by indirect ELISA and all of them showed above 1:400,000. 5. The layer of precipitate was observed on the zona incubated with medium containing 2% ascitic fluid of monoclonal antibody while the eggs treated with 2% normal mouse serum did not. 6. Porcine eggs incubated with medium containing 2% ascitic fluid of monoclonal antibody and followed by subsequent incubation with Rabbit anti-mouse IgG-FITC conjugate showed strong fluorescent light on the zona surface while the zona of normal mouse serum-treated eggs did not show fluorescence.

Decentering Korean Identity with Diasporic Art: A Conversation with Y. David Chung

Y. David Chung 고려대학교 민족문화연구원 2018 Cross-Currents Vol.0 No.29

In conversation with Hijoo Son, Phillips Academy, and Jooyeon Rhee, The Hebrew University of Jerusalem Y. David Chung is an artist and filmmaker known for his film and video works, installations, performances, drawings, prints, and public artworks. This interview combines two conversations on the concept of Korean identity and diasporic art: one that took place in 2008, after Chung finished filming his documentary Koryo Saram: The Unreliable People, co-directed with Matt Dibble, and the other in 2018. Hijoo Son and Jooyeon Rhee jointly designed the questions, interviewed Professor Chung, and redacted the transcript into its present form.

Comment on 'Enhancement in hardness and transmittance of ZnS via SiO₂/Y₂O₃ multilayer'

Chung, Y.D.,Cho, D.H.,Kim, S.B.,Han, W.S. Elsevier Sequoia 2016 Journal of Alloys and Compounds Vol.664 No.-

In a recent paper [1], Huayu Zhang et al. reported data on the enhancement in hardness and transmittance of ZnS via SiO<SUB>2</SUB>/Y<SUB>2</SUB>O<SUB>3</SUB> multilayer. In particular, the authors discussed the chemical state of sputtered Y<SUB>2</SUB>O<SUB>3</SUB> on SiO<SUB>2</SUB>/ZnS measured by X-ray photoelectron spectroscopy. We suggested that the split spectrum of Y 3d doublet should be decomposed into the sum of the doublet and the exact notation of O 1s level should be applied. The O 1s of 528.8 eV could also be assigned to Y<SUB>2</SUB>O<SUB>3</SUB> and the O 1s of 531.5 eV could be assigned to Si related compound.

F-free Y & Cu 전구용액 적용에 의한 YBCO coated conductors의 MOD 공정 개선

김영국,유재무,정국채,고재웅,조윤숙,허은옥,Kim, Y.K.,Yoo, J.M.,Chung, K.C.,Ko, J.W.,Cho, Y.S.,Heo, E.O. 한국초전도학회 2006 Progress in superconductivity Vol.8 No.1

Total Fluorine content in the precursor solution for MOD processing of YBCO coated conductors can be significantly reduced by synthesizing precursor solution with F-free Y & Cu precursor and Barium trifluoroacetate(TFA). It was shown that crack-free and uniform precursor films were formed after calcinations in humidified oxygen atmosphere. Less than 2 hours are required to finish the calcination process, and XRD measurement shows that $BaF_2,\;CuO,\;and\;Y_2O_3$ are major constituent of calcined precursor films. Film thickness after calcination was measured to be ${\sim}2.8$ um by applying slot-die coating method. In particular, addition of Samarium shows critical current of Ic=226 A/cm-w($Jc=3.4\;MA/cm^2$). Also discussed are recent developments in the reel-to-reel processing using F-free Y & Cu precursor solutions. It is shown that uniform and fast processing route to YBCO coated conductor with high Ic can be provided by employing F-free Y & Cu precursor solutions in MOD process.

표면탄성파 소자 응용을 위한 $LiTaO_3$ 단결정 성장

정대식,노용래,박병학,김유성,Chung, D.S.,Roh, Y.R.,Park, B.H.,Kim, Y.S. 한국결정성장학회 1994 한국결정성장학회지 Vol.4 No.2

표면탄성파 소자응요을 위하여 완전용융조성, 48.65 mole % $Li_2O에서 LiTaO_3(LT)$ 단결정(Y, X-axis)을 육성하였다. 육성된 $LiTaO_3(LT)$ 단결정의 SAW 소자성능을 확인하기 위하여 photolithography를 거쳐 기본적인 SAW filter를 제작하였다. Yamaju LT Y-cut 단결정 기판과 본 연구에서 제작된 RIST LT Y-cut 단결정 기판에서의 SAW특성결과를 비교하여 성능을 결정하였다. Yamaju LT 기판에서보다 본 연구에서 육성된 LT 단결정 기판에서 우수한 SAW 성능을 보였다. $LiTaO_3 (LT)$ single crystals (Y, X-axis) were grown from the congruent composition, 48.65 mole %, $Li_2O$ for SAW (Surface Acoustic Wave) applications. Basic SAW filters were fabricated on the RIST prepared LT wafers (Y-cut) using phtolithography. SAW filter performance was evaluated. The results were compared of the SAW characteristics between RIST prepared LT wafer (Y-cut) and commercial Yamaju wafer (Y-cut). The SAW filter prepared on the RIST grown LT wafer was shown better SAW performances than that of Yamaju wafer.

200 GeV/핵자 유황이온과 핵건판핵의 충돌에 의해 생성된 헬륨 파쇄핵의 극한파쇄 연구

김동철,송진섭,윤천실,정성헌,박인곤,김종오,김철수,김태연,이승희,조재희,천병구,김재률,김준원,김태익,박명렬,장한일,임인택 慶尙大學校 기초과학연구소 1992 基礎科學硏究所報 Vol.8 No.-

고에너지 중이온 원자핵과 핵건판의 충돌에서, 200GeV/핵자 유황이온에 의해 생성된 파쇄 헬륨핵(Z=2)의 실험실계의 방출각 분포는 표적핵에 무관한 회귀공식. dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)]로 잘 표현된다. 여기에서 의사신속도 η=-ln[tan(θ/2)]이고, y_b는 실험실계의 입사입자(^32S)의 신속도이다. 이 공식에 의한 적합에서 k=-0.057±0.008로 얻어진다. 즉, 핵건판과 고에너지 중이온의 충돌에서 파쇄 헬륨핵의 exp(η-y_b)의 분포는 "극한파쇄" 현상을 잘 설명하고 있다. The angular distribution of emission angle θ of helium (Z=2) produced in the collisions of incident particles of 200 GeV/nucleon ^32S in nuclear emulsion is well expressed by dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)] where the pseudorapidity is η=-ln[tan(θ/2)], the laboratory system primary rapidity is y_b, and k=-0.057+0.008. The shape of this frequency of occurrence distributions in terms of exp(η-y_b) attests to the validity of the concept of "limiting fragmentation" for helium projectile fragments produced in the projectile fragmentation regions of heavy ion collisions in nuclear emulsion.

Pretreatment HBeAg level and an early decrease in HBeAg level predict virologic response to entecavir treatment for HBeAg‐positive chronic hepatitis B

Kwon, J. H.,Jang, J. W.,Lee, S.,Lee, J.,Chung, K. W.,Lee, Y. S.,Choi, J. Y. Blackwell Publishing Ltd 2012 Journal of viral hepatitis Vol.19 No.2

<P><B>Summary. </B> There are few reports on hepatitis B e antigen (HBeAg) titres during nucleos(t)ide analogues treatment. We investigated the changes in HBeAg levels in patients treated with entecavir and the usefulness of HBeAg quantification for predicting antiviral response. Ninety‐five consecutive HBeAg‐positive patients treated with entecavir for more than 48 weeks were enrolled. Serum levels of hepatitis B surface antigen (HBsAg), HBeAg and HBV DNA were assessed at 4‐week intervals to week 24 and thereafter at 12‐week intervals. Virologic response (Y1VR) was defined as an undetectable HBV DNA level at week 48 of therapy. During 48 weeks, HBeAg and HBV DNA level decreased significantly in a biphasic manner and HBsAg level tended to decease. Fifty‐three patients (55.8%) attained Y1VR. Pretreatment HBeAg levels were significantly lower in the Y1VR group than in no Y1VR group. At week 4 and 12 of therapy, 25% and 41.4% of patients showed a decrease of HBeAg levels with >0.5 log<SUB>10</SUB> and >1.0 log<SUB>10</SUB> from baseline, respectively. These patients achieved more Y1VR than those with less decrease of HBeAg levels (97.7%<I>vs</I> 22.2% and 86.2%<I>vs</I> 29.3%, respectively). HBeAg level at week 12 had higher predictive values for Y1VR than HBV DNA level. Multivariate analysis revealed that a pretreatment HBeAg level of <360 PEIU/mL and the reduction in HBeAg level >1.0 log<SUB>10</SUB> at week 12 were associated with Y1VR. These results suggest that pretreatment HBeAg level and an early decrease in HBeAg level are useful measurements for predicting one‐year virologic response during entecavir treatment.</P>

수도작용 송풍식 입제살포기 개발에 관한 연구 : 입제 배출량, 분두 및 적정살포조건 Granular Discharge Rate, Diffuser and Optimal Application Conditions

정창주,정선옥,장영창,최영수,최중섭 한국농업기계학회 1997 바이오시스템공학 Vol.22 No.2

派越戰傷者의 神經外科的 考察

鄭炳權,李英注,崔東烈,朴東春,黃度允,朱文培,朴昌秀 中央醫學社 1972 中央醫學 Vol.22 No.5

The authors have analyzed 900 cases of Korean wounded veterans who have evacuated from Vietnam war field, chiefly on view of neurosurgical aspects, collected for last 5 years and 10 months; 1. There divided into 319 cases of head injuries, 315 cases of peripheral nerve injuries, 138 cases of vertebral and spinal cord injuries, and 128 cases of H. N.P. and others in the total 900 cases of the injured veterans. 2. In 319 cases of head injuries, 165 patients or 51.5% were injured from. fragment of shell, explosives, grenade and booby trap. 3. On the site of head injury, the frontal injury was most frequent in rate of 38.6% and next temporal and parietal in that order. Craniectomy was carried out on 187 cases (58.6%) of head injuries and cranioplasty on 165 cases. 4. 48. 1% of total head injuries were resulted into sequelae such as hemiplegia (14.4%), cranial nerve palsy, speech disturbance and epilepsy in that order. 5. In the larger proportions of the causes of the peripheral nerve injuries, fragment injury occupied 66.7% and bullet 26.0% and total cases divided into 8.8% brachial injury, 19.4% ulnar injury, 14.7% median, 15.9% radial, 19.8% sciatic, 10.0% peroneal and 7.7% tibial nerve injury. 6. On the other hand, 47. 1% of lumbar and 41.3% of thoracic injury were demonstrated and paraplegia in 24.6% of cord injury. 7. In 1.38 cases of vertebral and spinal cord injury 46.7% of cases were resulted from fragment injury, which was similar to cases of the bullet injury in rate of 46% in Korean conflict.

Bridging the Community Divide in Koreatown Politics

Angie Y. Chung 在外韓人學會 2010 在外韓人硏究 Vol.21 No.-

학계에서는 흑인과 라틴계열의 정치결속의 중요성은 인지하여 왔음에도 불구하고, 1965년 이후의 아시아계 이민사회가 세대 간 갈등, 계층 양극화, 주거이동성의 증가속에서 어떻게 정치적 결속을 유지할 수 있는지를 분석한 연구는 거의 없다. 이 연구의 주목적은 1992년 LA폭동 이후에 재미한인들이 코리아타운 밖으로 이주했음에도 불구하고 어떻게 이민 1.5/2세대 한인 기관들을 정치권력으로 키울 수 있었는가를 탐구하는 것이다. 로스엔젤레스의 경우에 재미 한인들은 자신들의 기업들과 단체들을 다문화적 코리아타운에서 확장시키면서도 글렌데일(Glendale), 토렌스(Torrance), 풀러톤(Fullerton), 세리토스(Cerritos)와 같은 외진교외지역으로 주거지역을 옮겨 나갔다. 이 연구의 일반적 전제는 코리아타운 전체적인 성장이 지역사회 기관으로 하여금 서비스를 통합하고, 자원을 공유하고, 긴급한 상황에 즉각적으로 단결할 수 있도록 하였다는 것이다. 이 연구는 5년간의 인종학적 관찰과 코리아타운의 단체장 및 그 회원들의 심층 인터뷰에 근거하여 이민1.5/2세대 코리아타운 지역단체들의 정치적인 출현에 기여해 온 다음의 4개의 요소를 집중적으로 다루고 있다. 1) 선거구의 분리에 직면하여 한인의 정치 기반을 집중시킬 수 있는 단체들의 필요성 증대, 2) 1992년 로스엔젤레스 폭동 이후 한인 이민자들과 그들 자녀들의 높아진 정치의식, 3) 폭동 이후의 대폭적인 경제적 성장과 코리아타운의 고급주택화, 4) 지역 이민자 대표와 다문화적 인구 구성 기반을 동반하여 일해 온 지역사회 의존적 한국계 미국인 단체들의 정치 전략. Although theorists have come to recognize the continuing significance of ethnic political solidarity for Black and Latino groups, there have been few studies that have analyzed how post.1965 Asian immigrant communities are able to sustain ethnic political solidarity amidst increasing generational cleavages, class polarization, and residential mobility. The main objective of the paper is to explore how 1.5/ 2nd generation ethnic organizations are able to strengthen their political power in the period after the 1992 Los Angeles Civil Unrest, despite the out.migration of Korean Americans from Koreatown. In the case of Los Angeles, Korean Americans have clearly suburbanized into outlying suburban neighborhoods such as Glendale, Torrance, Fullerton, and Cerritos, even as businesses and institutions continue to expand in the multicultural Koreatown enclave.The general premise of my argument is that the institutional growth of Koreatown has enabled community.based organizations to coordinate their services, share resources, and instantly band together in response to urgent events. Based on five years of ethnographic observation and in.depth interviews with community leaders and other Koreatown affiliates, the paper focuses on four contextual factors that have contributed to the political emergence of 1.5/ 2nd generation organizations in Koreatown, including: 1) the growing need for organizations to concentrate their political base in the face of electoral fragmentation; 2) the heightened political consciousness of Korean immigrants and their children after the 1992 Los Angeles Civil Unrest; 3) the substantial economic development and gentrification of Koreatown in the post.Riot era; 4) and the political strategies of community.dependent Korean American organizations that work closely with local immigrant leaders and their multiracial populationbase.