Effects of Biologically Active Substances in Natural Products on the Hepatic Detoxication Mechanism : In Vivo Production and Enzyme-Inducing activity of Indolo[3,2-b]carbazole

Kwon,Chong-Suk(권정숙),Grose,Karl R,Riby,Jacques,Chen,Yue-Hwa,Bjeldanes,Leonard F. 韓國營養學會 1994 Journal of Nutrition and Health Vol.27 No.4

韓國人에서 多重增幅 重合酵素反應으로 分析한 STRs 遺傳座位의 遺傳的 多樣性

권국환,이혜린,박종진,황적준,이희석,송은섭 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

The tetrameric STRs loci were studied in a population of Korean(n=223) for allele frequency distribution and applicability to identity and paternity testing, using multiplex PCR with electrophoresis of the PCR products in DNA sequencing gels and subsequent detection of allelic fragments by silver staining. The STRs loci analyzed were HUMCSF1PO, HUMTPOX, and HUMTHO1 ; in the Korean population, 9 alleles with their frequency range of 0.002-0.363 are detected in the HUMCSF1PO, 5 alleles with those of 0.020-1.480 in the HUMTPOX, and 6 alleles with those of 0.002-0.489 in the HUMTHO1. The highest observed heterozygosity is found at the locus HUMCSF1PO(0.709), those of the loci HUMTPOX and HUMTHO1 being 0.646 and 0.632, respectively. All loci meet Hardy-Weinberg expectations ; there are good agreement between the observed and expected values under Hardy-Weinberg equilibrium. Moreover, pairwise comparisons between loci show allelic independence for all the 3 loci comparisons. The power of discrimination (PD) determined for the locus HUMCSF1PO is 0.8896 (88.96%), that of the HUMTPOX is 0.8818(88.18%), and that for the HUMTHO1 is 0.8367(83.67%) ; the combined power of discrimination for the triplex is 0.9976(99.76%). The power of exclusion(PEX) calculated for the loci HUMCSF1PO, HUMTPOX, and HUMTHO1, being prior probability that a falsely accused father will be excluded, and 0.5173(51.73%), 0.4610(46.10%), and 0.5101(510.01%), respectively, and the cummulative power of exclusion(CPE) for all the three loci is 0.8726(87.26%). Thus, these allelic frequency data can be used to construct the database of the multiplex PCR-based DNA profile in the Korean population. The calculated parameter, "power of discrimination(PD)" and "power of exclusion(PEX)", show the informativeness of these loci for the determination of identity and relatedness of individuals.

PCB投與動物의 成長과 脂質代謝에 미치는 花紛粒의 影響

권정숙 安東大學 1986 安東大學 論文集 Vol.8 No.1

For the purpose of investigating the influence of pollen loads on growth and lipid metabolism in serum and tissues of PCB-administered rats, I treated some rats with different levels of PCB and others with additional pollen loads for 4 wks. Growth depression in PCB-administered rats was proportional to the PCB concentration, but was markedly improved by administrating pollen loads. Liver and kidney enlargement, hepatic fatty infiltration and hyperlipidemia in PCB-administered rats increased in proportion to the PCB concentration. However, pollen loads added to the PCB diet improved the toxicity symptoms by PCB. Furthermore, the symptoms by low PCB diet were completely disappeared by additional pollen load diets.

특정 염기변환 방법을 이용한 PU.1 유전자의 돌연변이체 제조

류종석,권무식 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2

The purine-rich sequence (GAGGAA) binding protein, PU.1, is one of the tissue specific transcription factors. It is related to the activation of macrophage & B-cell in mammals. It is postulated that the activation of PU.1 could be accomplished by the phosphorylation of serine(s). The PU.1 contains 22 serine residues in its coding region. In order to figure out a plausible phosphorylation site, one nucleotide Thymine of PU.1 cDNA coding the 126th amino acid serine (TCC) was substituted with Guanine by site-directed mutagenesis using polymerase chain reaction. The mutated PU.1 cDNA was ligated into a pBluescript KS+ and transformed into E. coli JM109. The mutant clone will be employed to elucidate the phosphorylation site(s) and to understand the phosphorylation mechanism of transcription factor, PU.1.

다발성 대장암 간전이 환자의 간절제를 위한 새로운 시도

주종우,김형철,임철완,신응진,조규석,유기원,송옥평,홍대식,박성진,조준희,이혜경,김희경,권계원,고은석 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Multiple bilobar liver matastases (MBLM) are the main cause of low resectability in the colon cancer liver metastases. The authors experienced one case of initially non-resectable colon cancer liver metastases. He was curatively and safely treated with a two-stage hepatectomy using the new method of future remnant liver volume growing. A 54-year-old man was referred to our department with the sigmoid colon cancer combined with MBLM, which were checked in two small metastatic lesions in the left lobe and five large sized lesions in the right lobe in the computed tomogram (CT). A laparoscopic assisted anterior resection was primarily performed. We performed the 1^(st) stage hepatectomy 3 weeks after the colon resection. Intra-operative Ultrasonogram (US) found 9 small superficial metastatic lesions in the left lobe. All that lesions were completely removed by non-anatomical wedge resection. An occlusion ballon catheter was placed in the right portal vein through a small branch of the inferior mesenteric vein at that time. The future remnant liver volume was sufficiently increased 3 weeks after the 1^(st) hepatectomy. A right hepatectomy was safely performed 22 days after the 1^(st) hepatectomy. The patient received a regional chemotherapy (interleukin2 based immuno-chemotherapy through hepatic artery) for 4 months, then received 9 cycles of systemic chemotherapy (biweekly Oxaliplation, leucovorin, plus 5-fluorouracil) without any recurrence evidence.

의과대학생 및 전공의들의 영양교육의 필요성에 대한 인식

장현숙,권정숙 대한영양의학회 1999 영양의학 Vol.1 No.3

염모제 도포방법에 따른 인체 적혈구의 산화스트레스 비교

김영철,심미자,권정숙 한국환경독성학회 2004 환경독성보건학회지 Vol.19 No.2

To ascertain the effects of hair dyeing application on oxidative stress in human, a mixture of permanent black colored hair dye with the same amount of oxidant containing 6% hydrogen peroxide was used. A hair dyeing with contacting the scalp(conventional dyeing) and a hair dyeing with 3 to 4mm away from the scalp(alternative dyeing) were applied to each 15 young healthy women. Blood was taken from the brachial vein at two sampling times, just before and 6 hours after the hair dyeing, and antioxidant enzyme activities and antioxidant contents were measured in red blood cells. After increased, malondialdehyde(MDA) contents for conventional dyeing group was shown to a tendence for more increased than alternative dyeing group. After dyeing, reduced glutathione(GSH) contents for conventional dyeing group was shown to a tendence of more decreased than alternative dyeing group. After dyeing, superoxide dismutase(SOD) and catalase(CAT) activities were significantly decreased in conventional dyeing group(p<0.01), however, SOD and CAT activities were not significantly decreased in alternative dyeing group. After dyeing, there was no significant decrease in glutathione peroxidase(GPX) activity both for conventional dyeing group and alternative dyeing group. Therefore, after dyeing, the degree of oxidative stress in red blood cells for alternative dyeing group was appeared to be lower than conventional dyeing group.

염모제 사용에 의한 인체림프구의 DNA 손상 변화

김영철,심미자,권정숙 한국환경독성학회 2004 환경독성보건학회지 Vol.19 No.1

To ascertain the effects of hair dyeing application on the DNA damage in human lymphocytes, a mixture of permanent black colored hair dye with the same amount of oxidant containing 6% hydrogen peroxide was used. A hair dyeing with contacting the scalp(conventional dyeing) and a hair dyeing with 3 to 4mm away from the scalp(alternative dyeing) were applied to each 15 young healthy women. Blood was taken from the brachial vein at two sampling times, just before and 6 hours after the hair dyeing, and tail extent moment(TEM) and tail length(TL) were measured by using a comet assay. After dyeing, TL was significantly increased in both conventional dyeing group and alternative dyeing group compared with before dyeing as an average of 47% and 28%, respectively, and TL for conventional dyeing group was higher than alternative dyeing group as an average of 1.2 fold. After dyeing, TEM was significantly increased in both conventional dyeing group and alternative dyeing group compared with before dyeing as an average of 192% and 76% respectively, and TEM for conventional dyeing group was significantly higher than alternative dyeing group as an average of 1.7 fold. Therefore, alternative dyeing application was induced to lower lymphocyte DNA damage than conventional dyeing application, and TEM was appeared to be a more sensitive tool for the measurement of lymphocyte DNA damage than TL in this study.

마분말을 첨가한 국수의 특성

정구민,권정숙 安東大學校 農業科學技術硏究所 1998 農業科學技術 硏究論文集 Vol.5 No.-

마첨가 국수의 개발을 위해 밀가루에 열풍건조마가루와 냉동건조마가루를 각각 2, 5, 10% 첨가하여 건국수를 만들고, 이들의 물리적 성질과 조리성질을 조사하였으며 관능검사를 실시하였다. 밀가루에 마가루를 첨가하면 국수의 폭은 0.5-4.8% 두께는 8.8-20.8% 줄어들었다. 국수의 색은 마가루의 갈변으로 다소 누렇게 변하였으며 첨가량이 클수록 심하였다. 마첨가로 국수의 강도와 휨성은 증가하였으며, 이에 따라 마국수는 밀가루 국수보다 취급이 용이할 것으로 생각된다. 국수의 조리 후 무게증가량과 조리부피는 마첨가량에 따라 별 차이를 보이지 않았으나, 조리손실은 마첨가 국수가 1.21-1.49g/25g으로 밀가루 국수의 1.15g/25g 보다 높았다. 이는 마의 수용성 식이섬유 함량(4.74-4.96%)이 밀가루(2.69%)보다 높기 때문일 것이다. 관능검사 결과, 마가루를 2-5% 첨가한 국수가 5.4-6.4점을 얻어 밀가루 국수의 5점보다 높았다. Dried noodles were prepared by blending wheat flour with 2, 5, 10% hot-air dried Chinese yam powder (HD) and freeze dried Chinese yam powder (FD), respectively. Adding yam powder decreased width and thickness of noodles by 0.5-4.8% and 8.8-20.8% respectively. Color of noodles were darkened with addition of yam powder due to probably enzymatic browning. Adding yam powder increased strength and flexibility of noodles. Weight gain and volume of noodle after cooking were not affected by adding yam powder, but cooking loss was increased from 1.15g/25g for wheat noodle to 1.21-1.47g/25g for yam-added noodles because yam powders contain more soluble dietary fiber(4.74-4.96%) than wheat flour(2.69%). In sensory evaluation, noodles added with 2-5% HD and FD scored 5.4-6.4 point whereas wheat flour noodle was 5.

Induction of Quinone Reductase, an Anticarcinogenic Marker Enzyme, by Medicinal Herb Extracts

Kwon, Chong-Suk,Kim, Ji-Hyeon,Son, Kun-Ho,Kim, Young-Kyoon,Lee, Jeong-Soon,Lim, Jin-Kyu,Kim, Jong-Sang The Korean Society of Food Science and Nutrition 2002 Preventive Nutrition and Food Science Vol.7 No.4

To search for novel cancer preventive agents, we assessed the quinone reductase (QR)-inducing activities of medicinal herb extracts in cultured murine hepatoma cells (hepalclc7 cells). Among 216 herb extracts tested in this study, 8 kinds of herbal extracts were found to induce QR activity in hepalclc7 cells by more than 2-fold when used at the concentration of 25 $\mu\textrm{g}$/$m\ell$. The methanol extracts of Aster koraiensis NK and Pulsatilla koreana Nakai induced QR by 252 and 223 % , respectively, at the concentration of 25 $\mu\textrm{g}$/$m\ell$. Most of the herbal extracts with QR inducing-activity increased the enzyme activity in a typical dose-dependent manner. The QR activity in BP$^{r}$ cl cells was induced move than 50 % by the extracts of Pulsatilla koreana Nakai, Inula helenium, Physalis alkekengi var, francheti (Masters) Makino, Chrysanthemum zawadskii var. latilobum Kitamuva, Auemisia keiskeana Miquel, Chfsanthemum boreale Makino. In conclusion, hlsatilla koreana Nakai, Aster koraiensis N.K, and Chfsanthemum zawadskii var. iatilobum Kitamura, which showed relatively high QR induction, merit further animal study to evaluate their potential as cancer preventive agents.