Porphyromonas endodontalis의 침투에 따른 혈관 내피세포의 유전자 발현

공희정,최경규,박상혁,이진용,최기운 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.6

During the last two decades, there has been an increasing interest in the impact of oral health on atherosclerosis and subsequent cardiovascular disease (CVD). To date, some periodontal pathogens including Porphyromonas gingivalis (P. gingivalis) have been reported to be relevant to CVD. Porphyromonas endodontalis (P. endodontalis), which shares approximately 87% sequence homology with P. gingivalis, is mostly found within infected root canals. However, recent studies reveal that this pathogen also resides in the dental plaque or periodontal pocket in patients with periodontitis. It has been shown that P. endodontalis invades human coronary artery endothelial cells (HCAEC) and coronary artery smooth muscle cells (CASMC). To evaluate whether P. endodontalis can participate in the progression of atherosclerosis and CVD, we examined the changes in transcriptional gene expression profiles of HCAEC responding to invaion by P. endodontalis in this study. The following results were obtained. 1. Porphyromonas endodontalis was invasive of HCAEC. 2. According to the microarray analysis, there were 625 genes upregulated more than two-folds, while there were 154 genes downregulated by half. 3. Upregulated genes were relevant to inflammatory cytokines, apoptosis, coagulation and immune response. Enhanced expression of MMP-1 was also noticeable. 4. The transcription profiles of the 10 selected genes examined by real-time PCR agreed well with those observed in the microarray analysis. Thus, these results show that P. endodontalis presents the potential to trigger and augment atherosclerosis leading to CVD. 본 연구에서는 Porphyromonas endodontalis와 죽상경화증 및 심혈관질환과의 관계를 알아보기 위해, P. endodontalis가 사람의 관상동맥 내피세포에 침투했을 때 나타나는 유전자 발현의 변화를 microarray와 real-time PCR로 측정하였고, 발현이 증가된 유전자 중에서 죽상경화증과 연관된 유전자들이 관련 KEGG pathway 상에서 유의성 있는 영향을 미치는지를 분석하여 다음과 같은 결과를 얻었다. 1. Porphyromonas endodontalis는 사람의 관상동맥 내피세포에 침투하였다. 2. Microarray 분석결과, 대조군보다 발현이 2배 이상 증가된 유전자는 625개였고, 1/2이하로 감소된 유전자는 154개였다. 3. 발현이 2배 이상 증가된 유전자 중에는 염증성 cytokine 및 chemokine, 세포자멸사, 혈액응고와 면역반응 관련 유전자들이 포함되었다. 4. Microarray 분석결과를 확인하기 위해 발현이 2배 이상 증가된 유전자 중에서 10개의 유전자를 선택하여 real-time PCR을 시행하였고, 그 결과 microarray에서와 마찬가지로 발현 정도가 대조군보다 모두 높게 나타났다. 따라서 P. endodontalis가 사람의 관상동맥 내피세포에 만성적으로 작용했을 때, 심혈관질환에서 중요한 부분을 차지하는 죽상경화증을 유발하는 위험 요소 중의 하나로 작용할 수 있을 것으로 판단된다. 이와 관련된 자세한 기전을 이해하기 위해서는 앞으로 더 많은 연구가 필요할 것으로 보인다.

기능성 수영복 가슴캡의 설계에 관한 연구

노정화,최혜선,도월희 대한가정학회 2003 Human Ecology Research(HER) Vol.41 No.8

This study aims to improve the comfort and fit of womens swimsuits. Based on the results of the survey, trial suits were designed. In terms of the breast cap, there are five variations based on the feature, size, and thickness and two variations according to the connection mode of the cap. Total 17 trial swimsuits were made, ease of movement and wearing comfort were evaluated. The results of this study were as follows: 1. In terms of movement according to the type of breast cap, the round type showed much less movement than the triangular type at the lwer part of the cap. According to size, the cap that was manufactured on the basis of brassiere cop size showed much less movement than the ordinary cap size in the same round type of cap. In terms of the connection mode between breast caps and swimsuits, there was less cap movement when they were connected by power nets compared to the ordinary models which were connected by conventional rope. 2. Analysis of the differences in wearing comfort. The round type proved to be more convenient than the triangular type at the lower circumference of the cape, the power net connection was rated more positively by the respondents.

한국 노인의 특성에 따른 맛 감지 능력의 차이에 관한 연구

박경애,이희주,박명숙,이정희,전상은,김종성,최스미 대한노인병학회 2002 대한노인병학회지 Vol.6 No.4

운동 유발성 횡문근 융해를 동반한 요로결석 2례

허진,최원혁,조진혁,함영희,홍정범,정성규,김현,허동 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Two men were admitted to hospital with flank pain, hematuria, which was diagnosised as ureteral stone. Elevation of aspartate aminotransferase (AST) without typical pattern of toxic hepatitis was observed. Careful history taking, several laboratory tests, abdominal and pelvis computered tomography was done. Findings from theses examinations supported the clinical diagnosis of ureteral stone complicated of exercised induced rabdomyolysis. Early recognization of rhabdomyolysis in clinical setting is important, because clinical manifestations have ranged from asymtomatic elevation of creatine kinase to acute renal failure which is a life threating medical emergency. Authors report two cases of exercised induced rhabdomyolysis initially admittied as ureteral stone managed with hydration

Importance of coronary artery dominance in children to determine coronary artery dilatation

Hee Joung Choi 대한소아청소년과학회 2019 Clinical and Experimental Pediatrics (CEP) Vol.62 No.6

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Effective detective quantum efficiency of two detectors in a prototype digital breast tomosynthesis

Choi, Seungyeon,Choi, Sunghoon,Lee, Donghoon,Kim, Ye-seul,Choi, Young-Wook,Kim, Hee-Joung Elsevier 2019 Optik Vol.178 No.-

<P><B>Abstract</B></P> <P>Digital breast tomosynthesis (DBT) system is a novel imaging modality that strongly depends on the detector performance. The effective detective quantum efficiency (eDQE) is a newly accepted system of performance analysis metric that solves the disadvantages of conventional DQE evaluations, which do not consider clinical operating conditions. To evaluate the eDQE, we used the direct-conversion based Anrad detector and the indirect-conversion based Dexela detector. Our aim is to study the performance of such detectors when they are mounted on a prototype DBT system. The prototype DBT system consists of a CsI(Tl) scintillator/CMOS-based flat panel digital detector and an a-Se based direct conversion detector developed by the Korea Electrotechnology Research Institute (KERI). The performance of the two detectors was compared on the basis of scatter fraction, transmission factor, effective modulation transfer function (eMTF), and effective normalized noise power spectrum (eNNPS). As a result, the Dexela detector is characterized by a decreasing eNNPS trend as the spatial frequency increases, whereas the Anrad eNNPS looks almost independent of the spatial frequency. The eMTF was found to be higher in the Anrad detector compared to that in the Dexela one for all frequencies. The eDQE values at 1 cycle/mm (low frequency) were 0.11 and 0.17 for the Anrad and Dexela detectors, respectively, and the values of the spatial frequency <SUB> f e D Q E ( 0.1 ) </SUB> , corresponding to eDQE = 0.1, were 1.25 and 2.81 cycles/mm for the Anrad and Dexela detectors, respectively. The Dexela detector also presented a higher contrast-to-noise ratios (CNRs) for all different sizes of μCa groups, thus demonstrating overall better eDQE results in imaging performance compared to the Anrad detector.</P>

Pediatric Extracorporeal Membrane Oxygenation in Korea: A Multicenter Retrospective Study on Utilization and Outcomes Spanning Over a Decade

Choi Yu Hyeon,Jhang Won Kyoung,Park Seong Jong,Choi Hee Joung,Oh Min-su,Kwon Jung Eun,Kim Beom Joon,Shin Ju Ae,Lee In Kyung,Park June Dong,Lee Bongjin,Chung Hyun,Na Jae Yoon,Choi Ah Young,Cho Joongbum 대한의학회 2024 Journal of Korean medical science Vol.39 No.3

Background: Over the last decade, extracorporeal membrane oxygenation (ECMO) use in critically ill children has increased and is associated with favorable outcomes. Our study aims to evaluate the current status of pediatric ECMO in Korea, with a specific focus on its volume and changes in survival rates based on diagnostic indications. Methods: This multicenter study retrospectively analyzed the indications and outcomes of pediatric ECMO over 10 years in patients at 14 hospitals in Korea from January 2012 to December 2021. Four diagnostic categories (neonatal respiratory, pediatric respiratory, postcardiotomy, and cardiac-medical) and trends were compared between periods 1 (2012–2016) and 2 (2017–2021). Results: Overall, 1065 ECMO runs were performed on 1032 patients, with the annual number of cases remaining unchanged over the 10 years. ECMO was most frequently used for post-cardiotomy (42.4%), cardiac-medical (31.8%), pediatric respiratory (17.5%), and neonatal respiratory (8.2%) cases. A 3.7% increase and 6.1% decrease in pediatric respiratory and post-cardiotomy cases, respectively, were noted between periods 1 and 2. Among the four groups, the cardiac-medical group had the highest survival rate (51.2%), followed by the pediatric respiratory (46.4%), post-cardiotomy (36.5%), and neonatal respiratory (29.4%) groups. A consistent improvement was noted in patient survival over the 10 years, with a significant increase between the two periods from 38.2% to 47.1% (P = 0.004). Improvement in survival was evident in post-cardiotomy cases (30–45%, P = 0.002). Significant associations with mortality were observed in neonates, patients requiring dialysis, and those treated with extracorporeal cardiopulmonary resuscitation (P < 0.001). In pediatric respiratory ECMO, immunocompromised patients also showed a significant correlation with mortality (P < 0.001). Conclusion: Pediatric ECMO demonstrated a steady increase in overall survival in Korea; however, further efforts are needed since the outcomes remain suboptimal compared with global outcomes.

Correlation between Cytokine and Chemokine levels and Clinical Severity in Children with Mycoplasma pneumoniae Pneumonia

Choi, Hee Joung,Kim, Yeo Hyang The Korean Society of Pediatric Infectious Disease 2019 Pediatric Infection and Vaccine Vol.26 No.1

Purpose: The aim of this study was to evaluate the relationships between cytokine and chemokine levels and the clinical severity of Mycoplasma pneumoniae pneumonia. Methods: A retrospective analysis of clinical and laboratory parameters were performed. Serum levels of interleukin (IL)-6, IL-8, IL-10, IL-18, interferon-${\gamma}$-inducible protein-10 (IP-10), macrophage inflammatory $protein-1{\beta}$, and tumor necrosis $factor-{\alpha}$ were measured. The severity of patients' clinical course and radiologic findings were also assessed. Results: Seventy-two patients (35 males and 37 females) with a median age of 3.9 years (range, 1-16 years) were enrolled. Patients with lobar pneumonia (n=29) had significantly higher C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and IL-18 values than those with broncho-interstitial pneumonia (n=43). However, the cytokine and chemokine values did not differ between the group that was treated with corticosteroids (n=31) and the one that was not (n=41). The CRP, ESR, lactate dehydrogenase (LDH), IL-18, and IP-10 values showed positive correlations with fever duration prior to admission. The CRP and ESR values were positively correlated with IL-18, and LDH, with IP-10 levels. Conclusions: CRP, ESR, LDH, IL-18, and IP-10 values were associated with the severity of the disease, manifesting lobar pneumonia or prolonged fever duration prior to admission.

Bulb Scale Removal on Vegetative Growth, Flowering, and Flower Quality of Lilium spp.

Hee-Jun Kim(김희준),Jeong-Man Kim(김정만),Dong-Chil Choi(최동칠),Joung-Sik Choi(최정식),Yeong-Geun Choi(최영근) 한국원예학회 2003 Horticulture, Environment, and Biotechnology Vol.44 No.6

Apparent life-threatening event in infancy

Choi, Hee Joung,Kim, Yeo Hyang The Korean Pediatric Society 2016 Clinical and Experimental Pediatrics (CEP) Vol.59 No.9

An apparent life-threatening event (ALTE) is defined as the combination of clinical presentations such as apnea, marked change in skin and muscle tone, gagging, or choking. It is a frightening event, and it predominantly occurs during infancy at a mean age of 1-3 months. The causes of ALTE are categorized into problems that are: gastrointestinal (50%), neurological (30%), respiratory (20%), cardiovascular (5%), metabolic and endocrine (2%-5%), or others such as child abuse. Up to 50% of ALTEs are idiopathic, where the cause cannot be diagnosed. Infants with an ALTE are often asymptomatic at hospital and there is no standard workup protocol for ALTE. Therefore, a detailed initial history and physical examination are important to determine the extent of the medical evaluation and treatment. Regardless of the cause of an ALTE, all infants with an ALTE should require hospitalization and continuous cardiorespiratory monitoring and evaluation for at least 24 hours. The natural course of ALTEs has seemed benign, and the outcome is generally associated with the affected infants' underlying disease. In conclusion, systemic diagnostic evaluation and adequate treatment increases the survival and quality of life for most affected infants.