Studies on Cocoon Filament Size Deviation in Multivoltine Breeds and Multivoltine ${\times}$ Bivoltine Hybrids of Silkworm, Bombyx mori L.

Rao, D.Raghavendra,Singh, Ravindra,Kariappa, B.K.,Dandin, S.B. Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.9 No.2

Variation in the size of the silk filament will determine the uniformity and quality of the silk reeled. In the present study, an attempt has been made to study the filament size variation in 6 multivoltine parental breeds and 9 multivoltine ${\times}$ bivoltine hybrids in all three seasons of a year. All multivoltine breeds and multivoltine ${\times}$ bivoltine hybrids showed variation in filament size throughout its length from the outer layer to inner layer. Results of the present study indicated that the size of the filament decreased from outer to inner layer. The decrease in filament size was sudden in some breeds/hybrids whereas it was gradual in other. Relationship between filament length to that of slope, average filament size to slope, and maximum filament size to slope was determined based on regression analysis. Regression analysis revealed significant positive correlation between slope vs average filament length (r=0.92$^{**}$) in multivoltine${\times}$bivoltine hybrids. Among parental breeds, 96C showed lowest slope of the curve (b-value: -0.00428) and 96A showed highest slope of the curve (b-value: -0.00269). Among 9 hybrids, PM${\times}$N $B_4$ $D_2$ recorded lowest slope value (b-value: -0.00328) and BL24${\times}$N $B_4$ $D_2$ showed highest value for slope of the curve (b-value: -0.00234). The breed 96C, which showed lowest slope value can be utilized for future breeding programmes to breed strains with less size deviation. Three multivoltine${\times}$bivoltine hybrids viz., PM${\times}$N $B_4$ $D_2$, 96E${\times}$CSR19 and BL67${\times}$CS $R_{101}$ , which showed less slope values (b-values: -0.00328, -0.00300 and -0.00297 respectively) can be utilized for commercial exploitation to produce uniform silk.k.

Genomic Analyses of Toll-like Receptor 4 and 7 Exons of Bos indicus from Temperate Sub-himalayan Region of India

Malik, Y.P.S.,Chakravarti, S.,Sharma, K.,Vaid, N.,Rajak, K.K.,Balamurugan, V.,Biswas, S.K.,Mondal, B.,Kataria, R.S.,Singh, R.K. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.7

Toll-like receptors (TLRs) play an important role in the recognition of invading pathogens and the modulation of innate immune responses in mammals. The TLR4 and TLR7 are well known to recognize the bacterial lipopolysaccharide (LPS) and single stranded (ssRNA) ligands, respectively and play important role in host defense against Gram-negative bacteria and ssRNA viruses. In the present study, coding exon fragments of these two TLRs were identified, cloned, sequenced and analyzed in terms of insertion-deletion polymorphism, within bovine TLRs 4 and 7, thereby facilitating future TLR signaling and association studies relevant to bovine innate immunity. Comparative sequence analysis of TLR 4 exons revealed that this gene is more variable, particularly the coding frame (E3P1), while other parts showed percent identity of 95.7% to 100% at nucleotide and amino acid level, respectivley with other Bos indicus and Bos taurus breeds from different parts of the world. In comparison to TLR4, sequence analysis of TLR7 showed more conservation among different B. indicus and B. taurus breeds, except single point mutation at 324 nucleotide position (AAA to AAM) altering a single amino acid at 108 position (K to X). Percent identity of TLR7 sequences (all 3 exons) was between 99.2% to 100% at nucleotide and amino acid level, when compared with available sequence database of B. indicus and B. taurus. Simple Modular Architecture Research Tool (SMART) analysis showed variations in the exon fragments located in the Leucine Rich Repeat (LRR) region, which is responsible for binding with the microbial associated molecular patterns and further, downstream signaling to initiate anti-microbial response. Considering importance of TLR polymorphism in terms of innate immunity, further research is warranted.

Keratinocyte growth factor and thiazolidinediones and linolenic acid differentiate characterized mammary fat pad adipose stem cells isolated from prepubertal Korean black goat to epithelial and adipogenic lineage

Reza, A. M.,Shiwani, S.,Singh, N. K.,Lohakare, J. D.,Lee, S. J.,Jeong, D. K.,Han, J. Y.,Rengaraj, D.,Lee, B. W. Springer Science + Business Media 2014 In vitro cellular & developmental biology Animal Vol.50 No.3

The study was conducted to know and investigate the mechanism involved during mesenchymal to epithelial transition to unravel questions related to mammary gland development in prepubertal Korean black goat. We, therefore, biopsied mammary fat pad and isolated adipose cells and characterized with stemness factors (CD34, CD13, CD44, CD106, and vimentin) immunologically and through their genetic expression. Furthermore, characterized cells were differentiated to adipogenic (thiazolidinediones and alpha-linolenic acid) and epithelial (keratinocyte growth factor) lineages. Thiazolidinediones/or in combination with alpha-linolenic acid demonstrated significant upregulation of adipo-Q, PPAR-gamma, CEBP-alpha, LPL, and resistin. Adipose stem cells in induction mixture (5 mu g/ml insulin, 1 mu g/ml hydrocortisone, and 10 ng/ml epidermal growth factor) and subsequent treatment with 10 ng/ml keratinocyte growth factor revealed their trans-differentiating ability to epithelial lineage. From 2 d onwards, the cells under keratinocyte growth factor influenced cells to assume rectangular (2-4 d) to cuboidal (8-10 d) shapes. Ayoub-Shklar stain developed brownish-red pigment in the transformed cells. Though, expressions of K8 and K18 were noted to be highly significant (p < 0.01) but expressions of epithelial membrane antigens and epithelial specific antigens were also significant (p < 0.05) compared to 0 d. Conclusively, epithelial transformations of mammary adipose stem cells would add up knowledge to develop therapeutic regimen to deal with mammary tissue injury and diseases.

Comparative Dielectric and Ferroelectric Characteristics of Bi0.5Na0.5TiO3, CaCu3Ti4O12, and 0.5Bi0.5Na0.5TiO3–0.5CaCu3Ti4O12 Electroceramics

Singh, L.,Yadava, S. S.,Sin, B. C.,Rai, U. S.,Mandal, K. D.,Lee, Y. Springer Science + Business Media 2016 Journal of electronic materials Vol.45 No.6

<P>The dielectric and ferroelectric characteristics of Bi0.5Na0.5TiO3 (BNT), CaCu3Ti4O12 (CCTO), and 0.5Bi(0.5)Na(0.5)TiO(3)-0.5CaCu(3)Ti(4)O(12) (BNT/CCTO) ceramics are compared. X-ray diffraction patterns confirmed the formation of single phase of all the ceramics after sintering at 950A degrees C for 15 h. Scanning electron microscopy images of the sintered ceramics reveal average grain sizes in the range from 200 nm to 2.5 mu m. Energy-dispersive x-ray mapping and x-ray photoelectron spectroscopy show the presence of the elements Bi, Na, Ca, Cu, Ti, and O with uniform distribution in the ceramics. BNT/CCTO exhibits high dielectric constant (epsilon (r) similar to 6.9 x 10(4)) compared with BNT (epsilon (r) similar to 0.13 x 10(4)) and CCTO (epsilon (r) similar to 1.68 x 10(4)) ceramics at 1 kHz and 503 K. The high dielectric constant of BNT/CCTO compared with BNT and CCTO is associated with a major contribution from grain boundaries, as confirmed by impedance and modulus analyses. The P-E hysteresis loop of all the ceramics measured at room temperature and 50A degrees C exhibited typical ferroelectric nature. The remanent polarization (P (r)) of BNT (1.58 mu C/cm(2)) and CCTO (0.654 mu C/cm(2)) ceramics are higher than that of BNT/CCTO (0.267 mu C/cm(2)) ceramic.</P>

Mating Behaviour in Mulberry Silkworm, Bombyx mori (L.)

Saheb N. M. Biram,Singh Tribhuwan,Kalappa H. K.,Saratchandra B. Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.10 No.2

Mating is an essential behavioural social event in the life cycle of silkworm, Bombyx mori (L.) for the perpetuation of population. A number of intrinsic and extrinsic factors and events of significant importance are involved in successful mating and egg deposition by an adult silk moth which besides biochemical, physiological and environmental factors also includes attraction of reproductively competent male and female moth for mating, duration and frequency of mating, age of moth at the time of mating, reuse of male moth in the production of eggs etc. An attempt has been made in this review article to elucidate briefly the behaviour of male towards female moth after eclosion, impact of duration and frequency of mating on egg deposition and oviposition, reuse of mated male moth in the production of quality and quantity eggs etc. in the silk-worm, B. mori and its significance in silkworm seed production.

Charge and mass transport properties of La₂Ni_0.95Al_0.05O_4.025+δ

Jeon, S.Y.,Singh, B.,Im, H.N.,Seong, K.P.,Song, S.J. Elsevier Sequoia 2014 Journal of alloys and compounds Vol.589 No.-

<P>In this work, mass and charge transport properties of acceptor doped lanthanum nickelate, La2Ni0.95Al0.05O4.025+delta d (LNAO), were analyzed. The thermal expansion, electrical conductivity and thermoelectric power of LNAO were measured as a function of temperature in 25-1000 degrees C range and oxygen partial pressure ( pO(2)) in -14 <= log (pO(2)/atm)6 <= -1 range. The average thermal expansion coefficient was 13.77 x 10 (6) K (1). The electrical conductivity was analyzed in relation to the thermoelectric power to elucidate the positive deviation of the activity coefficient of hole on the basis of the delocalized electron model. The thermoelectric power measurement shows a p-type to n- type transition. The chemical diffusion coefficient (D-chem) and surface exchange coefficient (ksurf.) were calculated by 4-probe DC conductivity measurement and ksurf. was slightly higher than ((D) over tilde Dchem). The best- estimated hole-mobility values showed very weak temperature dependence. The results were compared with the literature results on La2NiO4+ d. (C) 2013 Elsevier B.V. All rights reserved.</P>

Nasal immunization with mannan-decorated mucoadhesive HPMCP microspheres containing ApxIIA toxin induces protective immunity against challenge infection with Actinobacillus pleuropneumoiae in mice

Li, H.S.,Shin, M.K.,Singh, B.,Maharjan, S.,Park, T.E.,Kang, S.K.,Yoo, H.S.,Hong, Z.S.,Cho, C.S.,Choi, Y.J. Elsevier Science Publishers 2016 Journal of controlled release Vol.233 No.-

<P>The development of subunit mucosal vaccines requires an appropriate delivery system or an immune modulator such as an adjuvant to improve antigen immunogenicity. The nasal route for vaccine delivery by microparticles has attracted considerable interest, although challenges such as the rapid mucociliary clearance in the respiratory mucosa and the low immunogenicity of subunit vaccine still remain. Here, we aimed to develop mannan-decorated mucoadhesive thiolated hydroxypropylmethyl cellulose phthalate (HPMCP) microspheres (Man-THM) that contain ApxIIA subunit vaccine - an exotoxin fragment as a candidate for a subunit nasal vaccine against Actinobacillus pleuropneumoniae. For adjuvant activity, mucoadhesive thiolated HPMCP microspheres decorated with mannan could be targeted to the PRRs (pathogen recognition receptors) and mannose receptors (MR) of antigen presenting cells (APCs) in the respiratory immune system. The potential adjuvant ability of Man-THM for intranasal immunization was confirmed by in vitro and in vivo experiments. In a mechanistic study using APCs in vitro, it was found that Man-THM enhanced receptor-mediated endocytosis by stimulating the MR of APCs. In vivo, the nasal vaccination of ApxIIA-loaded Man-THM in mice resulted in higher levels of mucosal sIgA and serum IgG than mice in the ApxIIA and ApxIIA-loaded THM groups due to the specific recognition of the mannan in the Man-THM by the MRs of the APCs. Moreover, ApxIIA-containing Man-THM protected immunized mice when challenged with strains of A. pleuropneumoniae serotype 5. These results suggest that mucoadhesive Man-THM may be a promising candidate for a nasal vaccine delivery system to elicit systemic and mucosal immunity that can protect from pathogenic bacteria infection. (C) 2016 Elsevier B.V. All rights reserved.</P>

Review : Mating Behaviour in Mulberry Silkworm, Bombyx mori (L.)

( N. M. Biram Saheb ),( Tribhuwan Singh ),( H. K. Kalappa ),( B. Saratchandra ) 한국잠사학회 2005 International Journal of Industrial Entomology Vol.10 No.2

Mating is an essential behavioural social event in the life cycle of silkworm, Bombyx mori (L.) for the perpetuation of population. A number of intrinsic and extrinsic factors and extrinsic factors and events of significant importance are involved in successful mating and egg deposition by an adult silk moth which besides biochemical, physiological and environmental factors also includes attraction of reproductively competent male and female moth for mating, duration and frequency of mating, age of moth at the time of mating, reuse of male moth in the production of eggs etc. An attempt has been made in this review article to elucidate briefly the behaviour of male towards female moth after eclosion, impact of duration and frequency of mating on egg deposition and oviposition, reuse of mated male moth in the production of quality and quantity eggs etc. in the silkworm, B. mori and its significance in silkworm seed production.

Observation ofX(3872)inB→X(3872)Kπdecays

Bala, A.,Bhardwaj, V.,Trabelsi, K.,Singh, J. B.,Abdesselam, A.,Adachi, I.,Aihara, H.,Arinstein, K.,Asner, D. M.,Aulchenko, V.,Aushev, T.,Ayad, R.,Aziz, T.,Bahinipati, S.,Bakich, A. M.,Bansal, V.,Barbe American Physical Society 2015 PHYSICAL REVIEW D - Vol.91 No.5

Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types

Yashpal S. Malik,Kuldeep Sharma,Nirupama Vaid,Somendu Chakravarti,K. M. Chandrashekar,Sanjay S. Basera,Rashmi Singh,Minakshi,Gaya Prasad,Baldev R. Gulati,Kiren N. Bhilegaonkar,Awadh B. Pandey 대한수의학회 2012 JOURNAL OF VETERINARY SCIENCE Vol.13 No.3

The present study describes the genotypic distribution of rotaviruses (RVs) in an Indian bovine population with unexpectedly higher proportions of G3 alone or in combination of G8/G10. PCR-genotyping confirmed that 39.4% (13/33) of the prevalent RVs were the G3 type while 60.6% (20/33) were dual G3G10 or G3G8 types. P typing revealed that 93.9% (31/33) of the samples were P[11] while 6.1% (2/33) possessed a dual P[1]P[11] type. Sequence analysis of the VP7 gene from G3 strains viz. B-46, 0970, and BR-133 showed that these strains had sequence identities of 90.5% to 100% with other bovine G3 strains. The highest identity (98.9% to 100%) was observed with RUBV3 bovine G3 strains from eastern India. The G3 strains (B-46, 0970, and BR-133) showed 97.5% to 98.8% sequence homologies with the Indian equine RV strain Erv-80. Phylogenetic analysis demonstrated that G3 strains clustered with bovine RUBV3 and J-63, and equine Erv-80 G3. Overall, these results confirmed that the incidence of infection by RVs with the G3 genotype and mixed genotypes in the bovine population was higher than previously predicted. This finding reinforces the importance of constantly monitoring circulating viral strains with the G3 genotype in future surveillance studies.