젖산나트륨 첨가와 젖산 침지가 비엔나 소세지의 품질 및 관능상에 미치는 영향

김영환,박구부,진상근,신택순,박범영,김영직 한국축산학회 1995 한국축산학회지 Vol.37 No.4

This study was conduct to investigate the interactive effects of sodium lactate addition and lactic acid dipping on the shelf life of Vienna sausage. Three kinds of Vienna sausages were macwfacaured as the control (addition of potassium sorbate and dipping in fumaric acid solution), treatment A(addition of sodium lactate and dipping in fumaric acid solution) and treatment B(addition of sodium lactate and dipping in lactic acid). They were divided into two groups and one group was stored at 5℃ for 6 weeks and the other one at 20℃ for 4 weeks. The physico-chemical properties of sausages were exanuned during the storage time. $quot;Ihe results are summarized as follows: 1. At both storage temperatures, lactic acid contents of treatment A and B were significantly (P$lt;0.05) higher than that of control. As storage period passed, it was significantly (P$lt;0.05) decreased in all treatments. Also, the decreasing rates of control and at 20℃ were higher than those of treatment A, B and at 5℃. 2. Jelly strength was low in the order of control, treatment A and B at both storage temperatures. Jelly strengths of control and treatment A were higher at the end of the storage compared to at the initial stage of the storage but treatment B was lower. It was lower at 30℃ than at 5℃. 3. At both storage temperatures, treatment B and control showed significantly (P$lt;0.05) higher $quot;L$quot; value than that of treatment A but lower $quot;a$quot; value, $quot;b$quot; value was decreased in the order of control, treatment A and B. $quot;L$quot; and $quot;a$quot; value were significantly (P$lt;0.05) decreased with storage period in all treatments but $quot;b$quot; value showed a increasing tendency. Treatment B showed intense discoloration compared to other treatments at 20℃. 4. In sensory panel score, there was a difference between control and treatment B and the difference was more obvious at 20℃ than at 5℃. From the results mentioned so far, it was shown that, treatment A maintained similar product quality to control at both storage temperatures white treatment B only at 5℃.

Synthesis of silver nanoparticles in an eco-friendly way using <i>Phyllanthus amarus</i> leaf extract: Antimicrobial and catalytic activity

Ajitha, B.,Reddy, Y. Ashok Kumar,Jeon, Hwan-Jin,Ahn, Chi Won VSP 2018 Advanced powder technology Vol.29 No.1

<P><B>Abstract</B></P> <P>In the present study, silver nanoparticles (AgNPs) with a flower-like structure were synthesized through an easy, rapid and eco-friendly pathway using <I>Phyllanthus amarus</I> leaf extract. The obtained AgNPs were characterized using ultraviolet–visible (UV–Vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and transmission electron microscopy (TEM). In addition, the antimicrobial and catalytic activities of the bio-synthesized AgNPs were carried out. Our results indicated that the concentration of the Ag precursor and the volume of the leaf extract played key roles in the formation of the flower-shaped AgNPs. Morphology study confirms the shape of the obtained bio-AgNPs as flower like structure. This study also showed the presence of clear capping layers surrounding and apparently interacting with the nanoparticles. Moreover, our studies indicated this interaction to involve bio-organic capping agents in the leaf extract. UV–Vis absorption spectra confirmed the formation of AgNPs with an optimized size. The zeta (ζ) potential of the AgNPs attests the stability of the nanoparticles. FTIR spectra provided evidence for the presence of biomolecules responsible for the reduction as well as capping of the AgNPs. Finally, the bio-synthesized AgNPs were shown to be an excellent microbial activity against the selected pathogens and enhanced catalyst of the reduction of rhodamine B.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Phyllanthus amarus</I> is an exceptional sink for biosynthesis of silver nanoparticles. </LI> <LI> Biomolecules of leaf extract are found to play an active role in AgNPs formation. </LI> <LI> Zeta potential value attested the higher stability of biosynthesized AgNPs. </LI> <LI> Excellent antimicrobial and catalytic activities were obtained in an eco-friendly way. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

CHMP5 controls bone turnover rates by dampening NF-κB activity in osteoclasts

Greenblatt, Matthew B.,Park, Kwang Hwan,Oh, Hwanhee,Kim, Jung-Min,Shin, Dong Yeon,Lee, Jae Myun,Lee, Jin Woo,Singh, Anju,Lee, Ki-young,Hu, Dorothy,Xiao, Changchun,Charles, Julia F.,Penninger, Josef M. The Rockefeller University Press 2015 The Journal of experimental medicine Vol.212 No.8

<▼1><P>Greenblatt et al. show that deletion of CHMP5 in osteoclasts leads to increased bone resorption coupled with exuberant osteoblast activity, resembling an early onset form of human Paget’s Disease of Bone</P></▼1><▼2><P>Physiological bone remodeling requires that bone formation by osteoblasts be tightly coupled to bone resorption by osteoclasts. However, relatively little is understood about how this coupling is regulated. Here, we demonstrate that modulation of NF-κB signaling in osteoclasts via a novel activity of charged multivesicular body protein 5 (CHMP5) is a key determinant of systemic rates of bone turnover. A conditional deletion of CHMP5 in osteoclasts leads to increased bone resorption by osteoclasts coupled with exuberant bone formation by osteoblasts, resembling an early onset, polyostotic form of human Paget’s disease of bone (PDB). These phenotypes are reversed by haploinsufficiency for <I>Rank</I>, as well as by antiresorptive treatments, including alendronate, zolendronate, and OPG-Fc. Accordingly, CHMP5-deficient osteoclasts display increased RANKL-induced NF-κB activation and osteoclast differentiation. Biochemical analysis demonstrated that CHMP5 cooperates with the PDB genetic risk factor valosin-containing protein (VCP/p97) to stabilize the inhibitor of NF-κBα (IκBα), down-regulating ubiquitination of IκBα via the deubiquitinating enzyme USP15. Thus, CHMP5 tunes NF-κB signaling downstream of RANK in osteoclasts to dampen osteoclast differentiation, osteoblast coupling and bone turnover rates, and disruption of CHMP5 activity results in a PDB-like skeletal disorder.</P></▼2>

젖산나트륨 첨가와 젖산 침지가 소세지의 열처리 손실 , 염도 , 아질산염의 잔존량 , pH , 보수성 , 수분활성도에 미치는 영향

박구부,진상근,이한기,송또준,박태선,김영직 한국축산학회 1995 한국축산학회지 Vol.37 No.4

This study was conducted to investigate the interactive effects of sodium lactate addition and lactic acid dipping on the shelf life of Vienna sausage. Three kinds of vienna sausages were manufactured as the control (addition of potassium sorbate and dipping in fumaric acid solution), treatment A(addition of sodium lactate and dipping in fumaric acid solution) and treatment B(addition of sodium lactate and dipping in lactic acid). They were divided into two groups, and one group was stored at 5℃ for 6 weeks and the other one at 20℃ for 4 weeks. When stored for 6 weeks at 5℃, residual nitrite content of treatment B was significantly (P$lt;0.05) tower compared to treatment A and control. Cooking loss and salt content of control were significantly (P$lt;0.05) higher than those of treatment A and B. pH of treatment A was significantly (P$lt;0.05) higher than those of control and treatment B at 5℃ but it was lower in the order of treatment A, B and control during the storage at 20℃. Water Hold Capacity was significantly (P$lt;0.05) low in the order of treatment A, B and control at both storage temperatures. Aw(Activity of water) of control was significantly (P$lt;0.05) higher than those of treatment A and B at both storage temperatures.

Expression of mosquitocidal crystal protein genes in non-insecticidal Bacillus thuringiensis subsp. israelensis

Gill, S.S.,Oh, H.W.,Lee, D.W.,Roh, J.Y.,Park, H.W.,Jin, B.R.,Je, Y.H.,Kang, S.K. 東亞大學校附設遺傳工學硏究所 1998 遺傳工學硏究 Vol.- No.5

J.Y.ROH,H.W.PARK,Y.H.JE,D.W.LEE,B.R.JIN, H.W.OH,S.S.GILL AND S.K.KANG.1997. Bacillus thuringiensis NTB-1 isolated from soil samples in Korea produces ovoidal parasporal inclusions with proteins of approximately 24-40kDa in size. Although serological study indicated that the isolate has a flagella (H) antigen identical with subsp. israelensis, it seemed to be non-insecticidal against Lepidoptera and Coleoptera as well as Diptera. To investigate the activity of non-insecticidal B.thuringiensis transformed with insecticidal crystal protein genes, cryIVD and cytA genes of B. thuringiensis subsp. morrisoni PG-14, highly toxic to mosquito larvae, were introduced into the isolate NTB-1. The expression of mosquitocidal crystal protein genes in NTB-1 was characterized by SDS-PAGE analysis and electron microscopy. The results showed that crystalline inclusions of host, CryIVD and CytA were stably expressed in the transformant. However, the mosquitocidal activity of transformant was similar to that of B. thuringiensis subsp. kurstaki Cry-B harbouring cryIVD and cytA genes, demonstrating that a synergistic effect by an interaction of both introduced insecticidal and resident non-insecticidal crystal proteins was not observed.

K₃Li₂Nb_5O_15 단결정의 성장과 특성에 관한 연구

진병문,노지현,김태훈,김정남,김진수 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

The potassium lithium niobate (K₃Li₂Nb_5O_15) single crystals were growing in K_χLi_(1-χ)NbO₃(x=0.4∼0.6) chemical formular by the Czochralski method. Crystal growth is studied in two orientations with growth along a-axis and c-axis. We have subjected this crystal to x-ray diffraction studies and found that they are single-crystalline and belong to tetragonal system with the lattice parameters a=b=12.577Å and c=3.997Å. The temperature dependence of dielectric constant was measured in the region of the phase transition. Curie temperature and diffuseness of phase transition are influenced by composition concentration. The composition and cation distribution of ferroelectric TB-type niobate crystals has a strong influence on the ferroelectric properties. Growth condition, optical transmittance, etching pattern and dielectric properties are presented and discussed.

Synthetic secapin bee venom peptide exerts an anti-microbial effect but not a cytotoxic or inflammatory response

Kim, B.Y.,Lee, K.S.,Ok, M.,Jin, B.R. 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol. No.

<P>Our previous study demonstrated that the secapin peptide from the venom of the Asiatic honeybee (Apis cerana, AcSecapin-1) exhibits anti-fibrinolytic, anti-elastolytic, and anti-microbial activities. In the present study, we investigated the anti-microbial activity and cytotoxicity of a synthetic AcSecapin-1 peptide. Seven synthetic AcSecapin-1 peptides (AcSecapin-S1 to AcSecapin-S7) were synthesized based on the peptide sequence of AcSecapin-1. AcSecapin-S1, which consists of the 25-amino acid sequence identical to that of the mature AcSecapin-1 peptide, exhibited the highest anti-microbial activity against bacteria and fungi. This was followed by AcSecapin-S6, which was missing 10 N-terminal amino acids and 6 C-terminal amino acids from AcSecapin-Sl. Furthermore, AcSecapin-Sl was not cytotoxic and did not activate macrophages. Taken together, our data demonstrated that a synthetic AcSecapin-1 peptide could exhibit anti-microbial activity without producing a cytotoxic or inflammatory response, suggesting that this synthetic AcSecapin-1 peptide can be used as an anti-microbial agent for biomedical applications. (C) 2017 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.</P>

Conjugated Linoleic Acid 가 Myoglobin 의 산화안정성에 미치는 효과

박구부,진상근,주선태,예병화,이정일,문성실 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.6

Animal fed CLA have been reported to have reduced weight loss, improved growth and increased lean body mass. In this study, we have hypothesized that CLA prohibits myoglobin oxidation due to delay lipid oxidation, so that meat color could be improved during cold storage. Two trials were conducted to investigate the relationship between CLA and myoglobin oxidation. For the first trial, a total of 48 pigs were fed a diet including 5% CLA for 4 weeks before slaughter, and meat color (CIE L*, a*, b*) and lipid oxidation (TEARS; thiobarbituric acid reactive substances) were measured during 11 days of cold storage at 4℃. The simple correlation coefficients between CLA and a* value and TBARS were -0.283 (p=0.180) and -0.804 (p$lt;0.0001), respectively. This result suggested that CLA could reduce effectively the lipid oxidation, but had only a small effect on meat color. For the second trial, we made beef patties including 0.5%, 1% and 2% CLA, and the changes in meat color and oxymyoglobin concentration were investigated during 13 days of cold storage. The L* value increased with increasing the CLA level in patties, and the a* value of 1% and 2% CLA added patty was higher than others. Also, oxymyoglobin concentration was not quickly decreased with higher levels of CLA. These results imply that CLA could prevent the oxidation of myoglobin due to delay of the lipid oxidation.

Hierarchical 3D ZnIn₂S₄/graphene nano-heterostructures: their <i>in situ</i> fabrication with dual functionality in solar hydrogen production and as anodes for lithium ion batteries

Kale, Sayali B.,Kalubarme, Ramchandra S.,Mahadadalkar, Manjiri A.,Jadhav, Harsharaj S.,Bhirud, Ashwini P.,Ambekar, Jalinder D.,Park, Chan-Jin,Kale, Bharat B. The Royal Society of Chemistry 2015 Physical Chemistry Chemical Physics Vol.17 No.47

<P>Hierarchical 3D ZnIn2S4/graphene (ZnIn2S4/Gr) nano-heterostructures were successfully synthesized using an in-situ hydrothermal method. The dual functionality of these nano-heterostructures i.e. for solar hydrogen production and lithium ion batteries has been demonstrated for the first time. The ZnIn2S4/Gr nano-heterostructures were optimized by varying the concentrations of graphene for utmost hydrogen production. An inspection of the structure shows the existence of layered hexagonal ZnIn2S4 wrapped in graphene. The reduction of graphene oxide (GO) to graphene was confirmed by Raman and XPS analyses. The morphological analysis demonstrated that ultrathin ZnIn2S4 nanopetals are dispersed on graphene sheets. The optical study reveals the extended absorption edge to the visible region due to the presence of graphene and hence is used as a photocatalyst to transform H2S into eco-friendly hydrogen using solar light. The ZnIn2S4/Gr nano-heterostructure that is comprised of graphene and ZnIn2S4 in a weight ratio of 1 : 99 exhibits enhanced photocatalytically stable hydrogen production i.e. B6365 mmole h(-1) under visible light irradiation using just 0.2 g of nano-heterostructure, which is much higher as compared to bare hierarchical 3D ZnIn2(S4). The heightened photocatalytic activity is attributed to the enhanced charge carrier separation due to graphene which acts as an excellent electron collector and transporter. Furthermore, the usage of nano-heterostructures and pristine ZnIn2S4 as anodes in lithium ion batteries confers the charge capacities of 590 and 320 mA h g(-1) after 220 cycles as compared to their initial reversible capacities of 645 and 523 mA h g(-1), respectively. These nano-heterostructures show high reversible capacity, excellent cycling stability, and high-rate capability indicating their potential as promising anode materials for LIBs. The excellent performance is due to the nanostructuring of ZnIn2S4 and the presence of a graphene layer, which works as a channel for the supply of electrons during the charge-discharge process. More significantly, their dual functionality in energy generation and storage is quite unique and commendable.</P>

Differential cytotoxic effects of sodium meta-arsenite on human cancer cells, dental papilla stem cells and somatic cells correlate with telomeric properties and gene expression.

Jeon, Byeong-Gyun,Kumar, B Mohana,Kang, Eun-Ju,Maeng, Geun-Ho,Lee, Yeon-Mi,Hah, Young-Sool,Ock, Sun-A,Kwack, Dae-Oh,Park, Bong-Wook,Rho, Gyu-Jin Potamitis Press 2011 Anticancer research Vol.31 No.12

<P>We investigated the effects of sodium meta-arsenite (NaAsO(2)) on human cancer cells (MDA-MB-231, MCF-7 and U-87 MG), dental papilla tissue stem cells (DPSCs) and somatic cells [MRC-5 fetal fibroblasts and adult muscle cells (MCs)] by examining telomeric properties, endogenous reverse transcriptase (RT) activity and the expression of tumorigenesis-linked genes. Half maximal inhibitory concentration (IC(50)) values were higher in DPSCs and MCs, possessing longer telomere lengths when compared to cancer cells. Levels of telomerase and RT activity, and the expression of protein 53 (p53), B-cell lymphoma 2 (BCL2), nuclear factor kappa-light-chain-enhancer of activated B-cells (NFκB), transforming growth factor beta (TGFβ) and vascular endothelial growth factor (VEGF) were significantly lower in cancer cells following sodium meta-arsenite treatment, whereas the effect was absent or marginally detected in DPSCs and somatic cells. Collectively, sodium meta-arsenite effectively induced cellular cytotoxicity by inhibiting telomerase and RT activity, and down-regulating transcript levels in cancer cells with shorter telomere lengths, whereas more tolerance was evident in DPSCs and somatic cells possessing longer telomere lengths.</P>