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Nizwaside: a new anticancer pregnane glycoside from the sap of Desmidorchis flava
Hidayat Hussain,Ahmed Al-Harrasi,Muhammad Adil Raees,Najeeb Ur Rehman,Ahmed Al-Rawahi,Rene´ Csuk,Husain Yar Khan,Ghulam Abbas,Mohammed Abdullah Al-Broumi,Ivan R. Green,Ali Elyassi,Talat Mahmood 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.12
The sap from the succulent Desmidorchis flava (N.E.Br) Meve and Liede yielded a new pregnane glycoside, named nizwaside whose structure was established using 1D and 2D NMR techniques as well as mass spectrometry (ESIMS). Nizwaside was tested for anticancer, DPPH antioxidant, urease enzyme inhibition, a-glucosidase enzyme inhibition and acetylcholinesterase inhibition activities. Interestingly, nizwaside showed significant anti-proliferative effects on MDA MB231 breast cancer cells with an IC50 of 23.5 lg/ml. Moreover, nizwaside was more effective than Doxorubicin, a wellknown clinical anticancer drug, in suppressing MDA MB231 cell proliferation even at concentrations lower than that of Doxorubicin (75 lg/ml nizwaside vs. 100 lg/ml Doxorubicin). On the other hand, nizwaside showed relatively weak antioxidant activity with 15 % inhibition.
Javid Hussain,Najeeb Ur Rehman,Ahmed Al-Harrasi,Abdul Latif Khan,Tania Shamim Rizvi,Faryal Vali Mohammad,Mehjabeen,Liaqat Ali 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6
The plant species of genus Nepeta are used totreat various human diseases and for ornamental purposesas well. Nepethalate B (1) was isolated as a result ofphytochemical investigations of Nepeta clarkei and wassubjected in the present study for investigation of analgesic,anti-inflammatory, and central nervous system (CNS)depressant activities. The percentage inhibition in phase Iof the acetic acid induced writhing test of compound 1(dose of 0.1, 0.2 and 0.4 mg/kg of body weight) was 53.3,58.0 and 86.7 % respectively. These results were foundsignificantly higher (P\0.01) as compared to the negativecontrol. Moreover, the percentage inhibitions of threephases for 0.1, 0.2 and 0.4 mg/kg were higher than thevalues obtained for Aspirin (positive control). In formalintest, the percentage pain inhibition between 0 and 5 min(early phase) was 68.0, 25.5, and 75.5 % for 0.1, 0.2 and0.4 mg/kg intra-peritoneal doses of compound 1 respectively. In case of late phase (20–30 min) it was 63.0, 66.7and 48.1 %, respectively. In comparison to aspirin, overallpercentage inhibition of compound 1 was significantlyhigher in early and late phases. Interestingly, at all dosescompound 1 showed more potent anti-inflammatory effectsin terms of intensity and duration as compared to aspirin. The gross behavioral study of nepethalate B (1) was alsocarried out and the results revealed that it exhibited CNSdepression in the mice and showed a prominent decrease inlocomotor activity.
Khan, Abdul Latif,Bilal, Saqib,Halo, Boshra Ahmed,Al-Harrasi, Ahmed,Khan, Abdur Rahim,Waqas, Muhammad,Al-Thani, Ghanim Salim,Al-Amri, Issa,Al-Rawahi, Ahmed,Lee, In-Jung Informa UK (TaylorFrancis) 2017 Journal of plant interactions Vol.12 No.1
<P>Current study aimed at exploring the diversity of bacterial endophytes with Boswellia sacra and their role in copper (Cu) stress to tomato plants. Bacterial endophytes were belonged to Bacillus, Rhizobium and Paenibacillus, which were screened against Cu (0=10 mM) stress to dwarf and normal rice seeds. Among strains, Bacillus amyloliquefaciens BSL16 showed significantly higher bioremediation potential by accumulating high Cu and promoting growth of rice seeds. B. amyloliquefaciens BSL16 significantly increased growth of tomato plants during 2.5 mM Cu stress. Active colonization of BSL16 reduced the accumulation of Cu in leaf, shoot, and root, and in parallel up-regulated total protein contents in leaf and stem. Glutathione peroxidase and reduced glutathione contents were significantly higher and lipid peroxidation was lower in endophyte-treated tomato plants as compared to Cu treatment. The current results conclude that application of metal bio-accumulating bacteria can help in improving the plant growth of tomato plants during Cu stress.</P>
Bilal, Saqib,Shahzad, Raheem,Khan, Abdul Latif,Al-Harrasi, Ahmed,Kim, Chang Kil,Lee, In-Jung Elsevier Scientific Pub. Co 2019 Journal of Hazardous Materials Vol. No.
<P><B>Abstract</B></P> <P>This study investigates the stress-mitigating effects of endophytic <I>Penicillium funiculosum</I> LHL06 on soybean roots via modulation of physio-biochemical, molecular, and proteomic responses to combined heavy metal (Ni, Cu, Pb, Cr, and Al) toxicity. Preliminary screening revealed that LHL06 can tolerate and remediate combined heavy metal contamination in its media and upregulate gibberellins (GA1, GA3, GA4, GA7 and GA9) and indole-3-acetic acid (IAA) production. Inoculation of LHL06 resulted in marked reduction of metals uptake in roots and shoots by downregulating heavy metal ATPase genes (<I>GmHMA13, GmHMA14, GmHMA19)</I> and <I>GmMATE1</I> compared to non-inoculated plants; in turn, this decreased abscisic acid and jasmonic acid levels. Moreover, triggering of free amino acid metabolism in LHL06-inoculated roots significantly upregulated expression of stress-related proteins (glutathione S-transferase L3, isoflavone reductase-like, chalcone isomerase A, NAD(P)H dehydrogenase (quinone), FQR1-like 1 isoform X2, and Peroxidase 3) to combat metals toxicity. Compared to non-inoculated-plants, LHL06-inoculated-plants exhibited higher antioxidant activity and transcript accumulation of glutathione S-transferase (<I>GmGST8 and GmGST3</I>), <I>G6PDH</I>, and <I>GmSOD1[Cu-Zn]</I>, which decreased metal-induced reactive oxygen species. Therefore, LHL06-inoculation remediate combined metal contamination in soil, activate signaling network of stress-responsive hormones and antioxidant systems for promoting growth and tolerance, and reduce metal-accumulation, thereby making plants safer for consumption.</P> <P><B>Highlights</B></P> <P> <UL> <LI> LHL10 demonstrated significant resistance to heavy metals stress and produced higher gibberellins and indole-3-acetic acid. </LI> <LI> Association of LHL06 significantly alleviated negative effects of synergistic toxicity of heavy metals in soybean. </LI> <LI> Inoculated soybean demonstrated down-regulation of heavy metals transporters genes and illustrated reduced metals uptake. </LI> <LI> LHL06 increased Stress and defence-related proteins expression of soybean under synergistic heavy metals toxicity. </LI> <LI> LHL06-inoculated soybean exhibited lowered accumulation of endogenous hormones (ABA and JA) under stress. </LI> </UL> </P>
Abdul Latif Khan,Muhammad Waqas,강상모,Ahmed Al-Harrasi,Javid Hussain,Ahmed Al-Rawahi,Salima Al-Khiziri,Ihsan Ullah,Liaqat Ali,정희영,이인중 한국미생물학회 2014 The journal of microbiology Vol.52 No.8
Plant growth promoting endophytic bacteria have been identifiedas potential growth regulators of crops. Endophyticbacterium, Sphingomonas sp. LK11, was isolated from theleaves of Tephrosia apollinea. The pure culture of Sphingomonassp. LK11 was subjected to advance chromatographicand spectroscopic techniques to extract and isolate gibberellins(GAs). Deuterated standards of [17, 17-2H2]-GA4,[17, 17-2H2]-GA9 and [17, 17-2H2]-GA20 were used to quantifythe bacterial GAs. The analysis of the culture broth ofSphingomonas sp. LK11 revealed the existence of physiologicallyactive gibberellins (GA4: 2.97 ± 0.11 ng/ml) and inactiveGA9 (0.98 ± 0.15 ng/ml) and GA20 (2.41 ± 0.23). Theendophyte also produced indole acetic acid (11.23 ± 0.93μM/ml). Tomato plants inoculated with endophytic Sphingomonassp. LK11 showed significantly increased growth attributes(shoot length, chlorophyll contents, shoot, and rootdry weights) compared to the control. This indicated thatsuch phyto-hormones-producing strains could help in increasingcrop growth.
Endophytic fungi: resource for gibberellins and crop abiotic stress resistance
Khan, Abdul Latif,Hussain, Javid,Al-Harrasi, Ahmed,Al-Rawahi, Ahmed,Lee, In-Jung Informa Healthcare USA, Inc. 2015 Critical reviews in biotechnology Vol.35 No.1
<P>The beneficial effects of endophytes on plant growth are important for agricultural ecosystems because they reduce the need for fertilizers and decrease soil and water pollution while compensating for environmental perturbations. Endophytic fungi are a novel source of bioactive secondary metabolites; moreover, recently they have been found to produce physiologically active gibberellins as well. The symbiosis of gibberellins producing endophytic fungi with crops can be a promising strategy to overcome the adverse effects of abiotic stresses. The association of such endophytes has not only increased plant biomass but also ameliorated plant-growth during extreme environmental conditions. Endophytic fungi represent a trove of unexplored biodiversity and a frequently overlooked component of crop ecology. The present review describes the role of gibberellins producing endophytic fungi, suggests putative mechanisms involved in plant endophyte stress interactions and discusses future prospects in this field.</P>