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        In vivo evaluation of analgesic, anti-inflammatory, and neuropharmacological activities of the chemical constituent from Nepeta clarkei

        Javid Hussain,Najeeb Ur Rehman,Ahmed Al-Harrasi,Abdul Latif Khan,Tania Shamim Rizvi,Faryal Vali Mohammad,Mehjabeen,Liaqat Ali 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

        The plant species of genus Nepeta are used totreat various human diseases and for ornamental purposesas well. Nepethalate B (1) was isolated as a result ofphytochemical investigations of Nepeta clarkei and wassubjected in the present study for investigation of analgesic,anti-inflammatory, and central nervous system (CNS)depressant activities. The percentage inhibition in phase Iof the acetic acid induced writhing test of compound 1(dose of 0.1, 0.2 and 0.4 mg/kg of body weight) was 53.3,58.0 and 86.7 % respectively. These results were foundsignificantly higher (P\0.01) as compared to the negativecontrol. Moreover, the percentage inhibitions of threephases for 0.1, 0.2 and 0.4 mg/kg were higher than thevalues obtained for Aspirin (positive control). In formalintest, the percentage pain inhibition between 0 and 5 min(early phase) was 68.0, 25.5, and 75.5 % for 0.1, 0.2 and0.4 mg/kg intra-peritoneal doses of compound 1 respectively. In case of late phase (20–30 min) it was 63.0, 66.7and 48.1 %, respectively. In comparison to aspirin, overallpercentage inhibition of compound 1 was significantlyhigher in early and late phases. Interestingly, at all dosescompound 1 showed more potent anti-inflammatory effectsin terms of intensity and duration as compared to aspirin. The gross behavioral study of nepethalate B (1) was alsocarried out and the results revealed that it exhibited CNSdepression in the mice and showed a prominent decrease inlocomotor activity.

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