쑥갓의 생리활성 연구(Ⅱ)-STZ에 유도된 흰쥐에서의 과산화지질 생성계에 미치는 영향

정운용,천현욱,최종원,이용범,황기욱 慶星大學校 環境問題硏究所 1998 環境硏報 Vol.8 No.1

Chrysanthemum coronarium L. has long been used the appetite and constipation in oriental countries. We was investigated the effect on changes in serum and hepatic free radical generatin enzymes and free radical scavenging enzymes as well as detoxification mechanism of each fraction from Chrysanthemum coronarium by examining the induction of streptozotocin.(STZ) Male Sprague-Dawley rats(190∼210g), with free access to commercial rat chow (Cheil Foods & Chemical Inc.) and water, were divided into six groups : Group 1 served as normal; Group 4 received ethyl acetate fraction in STZ animal; Group 5 received butanol fraction in STZ animal; Group 6 received water fraction in STZ-induced rat. In the STZ-induced diabetic group, the serum superoxide dismutase activity decreased and formation of hydroxyl radical increased when dompared to normal. STZ caused increase hepatic lipid peroxide content was inhibited by treatment with Chrysanthemum coronarium butanol fraction and STZ decreased the hepatic glutathione compared to normal, but this was inhibited Chrysanthemum coronarium butanol fraction treatment.STZ induced change in hepatic microsomal cytochrome P-450 activity was inhibited by treatment with Chrysanthemum coronarium butanol fraction. STZ caused a significant rise in hepatic aniline hydroxylase and xanthine oxidase, decrease in aminopyrine N-demethylase when compared to normal. Activities of hepatic superoxide dismutase, catalase and glutathione peroxidase were significant rised by treatment with Chrysanthemum coronarium butanol fraction when compated to STZ. These results suggest that Chrysanthemum coronarium butanol fraction treatment suppressed STZ induced changes in serum and hepatic metabolizing enzymes system.

철도SOC사업투자계획 방안 제안

황기욱(Kiwook Hwang),엄광용(Kwangyong Um),김종민(Jongmin Kim),김민지(Minji Kim),이현희(Hyunhee Lee),박정수(Jungsoo Park) 한국철도학회 2012 한국철도학회 학술발표대회논문집 Vol.2012 No.5

현재 우리나라는 SOC사업을 통해 전 국토의 균형발전을 목표로 일반철도망과 고속선을 구축하여 빠른 경제발전을 이루고 있다. 하지만 고속선인 경부선을 중심으로한 일획적 발전으로 인해 지역 발전의 불균형이 일어나게 되었고 불가피하게 항만도시를 중심으로 경제발전이 이루어지고 있다. 이러한 문제점을 검토하기 위해 철도학술동아리 T.R.M에서는'오송역'을 중심으로 청주공항, 충주를 포함하여 제천까지를 잇는 충북고속선 연장으로 역세권개발 및 기업지구의 분배를 하므로 종단방향의 수도권-경남과 횡단방향의 경남-충북-충남의 KTX고속철도선 X축 형성으로 더욱 균형적인 발전을 도모하기 위하여 이번 충청지역 철도 SOC사업투자계획을 제안하고자 한다.

급성 Alcohol 중독시 뇌중 활성산소 생성에 미치는 영향

김동훈,최종원,황기욱,김회영,김상돈,이희정 慶星大學校 環境問題硏究所 1996 環境硏報 Vol.6 No.1

On the basis of some animal experiments it was generally until 20 years ago that liver disease of the alcoholic was due exclusively to malnutrition and not to direct toxic effects of alcohol itself. The presnet study was undertaken to investigate the following questions: whether ethanol added in vitro to isoated brain homogenates enhances oxygen radical formation, whether acute alcohol consumtion produces an increase in level oxygen radical in vrain and the importance of lipid peroxide changes in the brain of acute alcohol-fed mice and possible imbalance between generation and scavenger systems of free radical. The following observations were made: 1) Acute alcohol treated group enhanced the level of brain lipid peroxide conxentration. 2) Alcohol induced hepatic microsomal cytochrome P-450 of content and aminopyrine N- ndemet-hylase and aniline hydroxylase activities increased. 3) Xanthine oxidase and aldehyde oxidase were not affexted by alcohol treatment. 4) Superoxide dismutase, catalse and glutathine peroxidase were not affexted by alcohol treatment. 5) Hepatic cytosolic glutathione S-transferase activity was decreased by alcohol treatment. 6) ?-Glutamyl cystein synthetase activity was dexreased and glutathione reductase activity was not significantly efffected by alcohol treatment. 7) At in vitro test, alcohol and acetaldehyde added in test tube, acetaldehyde was significantly increased the level of brain lipid peroxide concentration and alcohol was not affexte. These results suggest that the mechanism of free radical formation at brain for the acute alcoho-treated if it were by due to generating and scavenging system, it's effext caused as a result of acetaldehyde.

운동부하시 간장중 지질과산화의 생성에 미치는 영향

김동훈,최종원,황기욱,김동영,이희정,김상돈 慶星大學校 環境問題硏究所 1996 環境硏報 Vol.6 No.1

It is wellknown that changes in physical fitness of an individual have profound physiological and biochemical effects on cardiovascular and skeletal muscle functions. Alterations in the concentration of hormones have also been associated with physical training programs. However, little known about the effects of physical training on the functional capacity of the hepatic drug metabolising enzyme system. We have studied the mechanism of lipid peroxidation formation by examing the induction of physical exercise. In blood, lactate dehydrogr\enase activity and pyruvate concentration in male mice exercised with physical training were significantly increased in comparison with control. Also, the concentration of lipid peroxide in hepatic tissue was significantly increased in comparison with control. Hepatic microsomal aminopyrine N-demethylase and aniline hydroxylase activities were not significantly different from any other experimental group. Cytosolic xanthine oxidase and aldehyde oxidase activities of physical exercise group were significanrtly increased in comparison with those of control group. Glutathione S-transferase, glutathione reductase and γ-glutamyl cystein synthetase activities were not significantly different from any other experimental group. The activities of superoxide dismutase, catalase and glutathione peroxidase which are related to the free radical scavenging system were significantly decreased in physical exercise group. Consquently, it was suggested that the mechanism of lipid peroxide formation for the physical exercise if it were by reason of free radical generating and scavenging system.

당뇨성 흰쥐에서 승홍의 신독성 생성에 관한 연구

정운용,천현욱,김동훈,황기욱,최종원 慶星大學校 環境問題硏究所 1997 環境硏報 Vol.7 No.1

We was investigated the effect on mechanism of nephrotoxicity formation of mercuric chloride by examing the induction of hyperglycemic by streptozotocin(STZ). Male Sprague-Dawley rats(190∼210g), with free access to commercial rat chow (Cheil Foods & Chemical Inc.) and water, were divided into four groups : Group 1 served as STZ; Group 2 received mercuric chloride in hypergycemic animal; Group 3 received mercuric chloride only; Group 4 served as normal. Mercuric chloride treatment in STZ diabetic rats inhibited the rise of BUN, lactate dehydrogenase and γ-glutamyltransferase activities in urine. Activities of renal xanthine oxidase and aldehyde oxidase were significant rised by treatment with mercuric chloride in STZ-induced hyperglycemic rats, the renal superoxide dismutase, catalase and glutathione peroxidase activities significantly decresed when compared to diabetic group. Concentration of renal tissure was significant increased by treatment with mercuric chloride in STZ-induced diabetic group when compated to diabetic group. These results suggest that mercuric chloride treatment supresses STZ induced changes in nephrotoxicity and the renal metablizing enzymes system.

서양고추냉이 추출물과 분리한 Kaempferol 배당체들의 브로모벤젠 처리 흰쥐에서 in Vitro 지질과산화억제효과

허종문,이종호,최종원,황기욱,정신교,김문성,박종철,Hur, Jong-Moon,Lee, Jong-Ho,Choi, Jong-Won,Hwang, Gi-WUk,Chung, Shin-Kyo,Kim, Moon-Sung,Park, Jong-Cheol 한국생약학회 1998 생약학회지 Vol.29 No.3

Three flavonoid glycosides have been isolated from the aerial part of Armoracia rusticana P. (Cruciferae) in Korea and identified by means of spectral analysis as $kaempferol-3-O-{\beta}-D-xylofuranoside(l)$, $kaempferol-3-O-{\beta}-D-galactopyranoside(2)$ and $kaempferol-3-O-{\beta}-D-xylofuranosyl(1\rightarrow2)-b{\beta}-D-galactopyranoside(3)$. When 1 mg/ml of the methanol extract from the aerial part of this plant was added, lipid peroxide formation in the bromobenzene-treated rat liver decreased by 64%. Among the components isolated from title plant, compounds 2 and 3 reduced the formation of lipid peroxide by 16% and 39% respectively at the concentration of ${10}^{-1}$ mg/ml.