LC-MS/MS를 이용한 인체시료 중 프탈레이트 대사체 동시분석법 확립

홍순근,남혜선,정기경,강일현,김태성,조상은,정수희,이장우,김준철,고영림,강태석,Hong, Soon-Keun,Nam, Hye-Seon,Jung, Ki-Kyung,Kang, Il-Hyun,Kim, Tae-Sung,Cho, Sang-Eun,Jung, Su-Hee,Lee, Jang-Woo,Kim, Jun-Cheol,Kho, Young-Lim,Kang, Tae-Se 한국환경보건학회 2010 한국환경보건학회지 Vol.36 No.6

Phthalates, such as di (2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) have been proved to be teratogenics and endocrine disruptors, metabolized rapidly and excreted in the urine. In this study, a simultaneous analytical method for 10 phthalate metabolites, MnBP, MiBP, MBzP, MCHP, MEHP, MEHHP, MEOHP, MnOP, MiNP and MiDP, in human urines, based on switching system with on-line pretreatment column using HPLC-MS/MS has been developed. This method was validated according to the guideline of bioanalytical method validation of National Institute of Toxicological Research. Limits of detection range between 0.2 and 0.9 ng/ml for 10 phthalate metabolites. The calibration curves showed linearity in the range 0.997~0.999, and the results of the intra- and inter-day validations were in the range from 0.4 to 14.7% RSD and from 0.3 to 9.4% RSD, respectively. Recoveries of phthalate metabolites varied from 87.0 to 116.1%. This analytical method showed high accuracy and stable precision for all metabolites, and seems to be suitable for biomonitoring of phthalates in human urine.

Studies on the Sfety of Korean Ginseng Ingested as Food Substance

홍순근,윤한교,장규섭,Hong, Soon Keun,Yoon, Han Kyo,Chang, Kyu Seob Institute of Agricultural Science 1984 農業技術硏究報告 Vol.11 No.2

In order to study the safety of ginseng ingested as a food substance, rats were fed ginseng added feed (subgroup 1: 0.625g/kg feed; subgroup 2: 1.25g/kg feed; subgroup 3: 2.5g/kg feed) for periods up to 6 months. Growth rates, blood composition, blood cell counts, and histopathological studies were carried out on both the control and test animals to study the effect of ingested ginseng. Following results were obtained: 1. The growth rates of the test and control animals were similar with the exception of the subgroup 3 in the 2-month fed animals who had a lower weight gain, and the subgroup 2 in the 4-month fed group who had a higher weight gain than the controls. 2. Liver, spleen, kidney, brain, lung and heart weights were similar between the test and the controls. 3. RBC, WBC, hematocrit, and hemoglobin values were similar between the test and control animals. the blood composition determination showed a similar level of alkaline phosphatase, triglyceride, total cholesterol, S-GOT, free cholesterol between the test and controls. However, free fatty acid, total lipid and S-GPT levels decreased while phospholipid, total protein, and HDL-cholesterol levels tended to increase. 4. Fl, F2 generations showed no abnormai values in blood count, organ weight and external appearance. 5. No histopathological differences were observed between the test and control animals even after a long-term administration of ginseng. However, there was evidence that ginseng promotes hematopoiesis in test animals. 6. These results suggest that ginseng is not only safe as a food substance but may indeed strengthen the body and help-prevent diseases of old age.

정보시스템 수용의 외생적 및 내재적 동기의 효과에 대한 개인적 관여도의 조절효과

홍순근(Soon-Geun Hong) 국제e-비즈니스학회 2008 e-비즈니스 연구 Vol.9 No.3

Internal and External Beliefs as the Determinants of Use Continuance for an Internet Search Site

홍순근(Soon-Geun Hong) 국제e-비즈니스학회 2010 e-비즈니스 연구 Vol.11 No.5

인삼 사포닌의 High Performance Liquid Chromatography에 의한 분리

홍순근(Soon Keun Hong),박은규(Eun Kyue Park),이춘영(Choon Young Lee),김명운(Myong Un Kim) 대한약학회 1979 약학회지 Vol.23 No.3,4

A high performance liquid chromatograpic procedure is described for determining ginseng saponins such as ginsenoside-Rb1, -Rb2, -Rc, -Rd, -Re, -Rf, -Rg1, and-Rg2. Ginseng saponins extracted with 90% methanol and water-saturated butanol were compared with pure standard ginsenosides. The resolution of the saponins was satisfactory and detection limit for each saponin was about 5mcg. Separation of the saponins was accomplished using a mc Bondapak carbohydrate analysis column, mobile phase of acetonitrile-water-butanol (80:20:15) and differential refractive index(RI) detector. The reproducibility and the recovery were also studied. This method was applied for determining the saponin contents of several parts of leaf, fresh ginseng, white ginseng, and red ginseng.

인삼(人蔘) 각(各) Fraction이 Nembutal, Chlorpromazine 및 Reserpine 투여(投與)로 인(因)한 Mouse체온하강(體溫下降)에 미치는 영향(影響)

홍사악(Hong, Sa-Ack),조항영(Cho, Hang-Young),홍순근(Hong, Soon-Keun) 대한약리학회 1969 대한약리학잡지 Vol.5 No.2

Saponin, essential oil, fat oil and alkaloidal fraction were fractionated from the ethanol extract of fanax ginseng. Effect of each fraction of Panax ginseng upon the temperature response induced by reserpine, chlorpromazine and nembutal was investigated in mice, so as to secure some hidden facets of each fraction of Panax ginseng acting upon central nervous system. The authors could arrive at some results, that is: (1) Inhibitory effect of Panax ginseng upon temperature decline induced by nembutal and chlorpromazine and potentiating action upon hyperthermia induced by reserpine reside mainly in saponin fraction and slightly in essential oil fraction. (2) The effect of Ginseng saponin on temperature response in nice seems to be related with the liberation of serotonin and histamine.

인삼(人蔘)사포닌의 동물행동(動物行動)에 대(對)한 작용(作用)

홍사악(Hong, S.A.),박찬웅(Park, C.W.),김재훈(Kim, J.H.),홍순근(Hong, S.K.),장현갑(Chang, H.K.),김명석(Kim, M.S.) 대한약리학회 1974 대한약리학잡지 Vol.10 No.2

Results of an experiment on the behavior of rats and mice in order to explore the possible pharmacological actions of Panax ginseng upon the central nervous system can be summarized as follows: 1. Spontaneous motor activity. In the case of mice, those groups who were administered 2.5 mg and 5.0mg of ginseng saponin per kilogram of body weight were observed to have increased their activity compared with the control group, while the 50.0 mg and 100.0 mg per kilogram body weight groups demonstrated lower levels of activity, with the peak of activity appearing at 30 minutes after administration of drugs. In the case of rats, those groups of animals who were given injections in the dosage of 2.5 mg, 5.0 mg and 50.0 mg per kilogram body weight demonstrated higher activity than the control group, while the 100.0 mg per kilogram group appeared to have decreased in their activity, with the peak action appearing 30 minutes after the administration of ginseng saponin. The 50.0 mg per kilogram group demonstrated no significant differential. 2. General behavior analysis. In the case of mice, decrease in sleeping component of behavior and increase in the walking and roaring components, compared those with the control group, turned out to be a common phenomenon among the groups who were administered 2.5 mg, 5.0 mg and 50.0 mg of ginseng saponin per kilogram body weight, with the 5.0 mg per kilogram group standing out of all the other groups in terms of their reactions. In the case of rats, ginseng saponin appeared to reduce sleeping component with 2.5 mg, 5.0 mg and 50.0 mg per kilogram body weight groups, while increased the walking and rearing components. It was observed that administratoin of ginseng saponin in a dose of 2.5 mg per kilogram appeared to markedly increase the lying and grooming components of animal behavior. 3. Open-field exploratory behavior. Adminstration of ginseng saponin to mice in doses of 5.0 mg, 50.0 mg and 100.0 mg per kilogram body weight decreased activity, but increased their exploratory behavior. In the case of rats, however, administration of ginseng saponin in the doses of 2.5 mg and 5.0 mg per kilogram body weight markedly increased their activities, while decreased activities with the 50.0 mg per kilogram and 100.0 mg per kilogram groups. The exploratory behavior of rats appeared to have decreased, while grooming increased ramarkably. 4. The above findings from a series of experiment appear to suggest a stimulating effect on the central nervous system when ginseng saponin is administered in small doses, but that larger doses might result in an inhibitory effect, though differential results can be anticipated with modification of experimental conditions.

Studies on the Sfety of Korean Ginseng Ingested as Food Substance

Soon Keun Hong(洪淳根),Han Kyo Yoon(尹漢敎),Kyu Seob Chang(張奎燮) 충남대학교 농업과학연구소 1984 農業技術硏究報告 Vol.11 No.2

직장인의 SNS 사용 분석을 통한 SNS 기획

김은주,홍순근,황찬규,Kim, Eun-Ju,Hong, Soon-Geun,Hwang, Chan-Gyu 한국전자통신학회 2013 한국전자통신학회 논문지 Vol.8 No.9

After platform changed from PC-based internet to mobile, SNS became new interactive media which relaces face-to-face interaction. the SNS users have already begun to recognize SNS as daily necessity. SNS market has been subdivided. In other words, SNS has entered into a period of vertical SNS that focus on contents and specific target. Therefore, It is necessary to analyze users for SNS planners. For this reason, analyzing why office workers who have the most powerful purchasing power use SNS is meaningful for SNS planners. Therefore, in this study, we analyzed the reasons for using SNS of office workers by studying relationship among office workers' stress, social support, self-expression and the use of SNS. As a result, the use of SNS has a significantly positive correlation with social support and self-expression. The self-expression in the SNS is not associated with stress, but rather it is the characteristics of the office workers. However the social support in the SNS affects to stress. PC기반의 인터넷에서 모바일로 플랫폼의 변화가 일어난 후 SNS가 대면 교류를 대체하는 새로운 대화 매체로 자리를 잡았다. 사용자들은 이미 SNS를 생필품으로 인식하기 시작했으며 나아가 SNS시장은 세분화 되고 있다. 즉, 특정 대상과 컨텐츠에 집중한 수직적(Vertical) SNS의 시대로 접어들었다고 볼 수 있다. 따라서 SNS기획에서 명확한 사용자 분석이 필요하며 이러한 시점에서 가장 구매력이 있는 직장인들이 SNS사용 이유를 살펴보는 것은 SNS사업자 입장에서는 의미가 있다. 이에 본 연구에서는 'SNS사용과 자기표현 및 사회적 지지와 직장인의 스트레스와의 관계'를 살펴봄으로써 직장인의 SNS사용 이유를 분석하였다. 그 결과 SNS 사용은 자기표현 및 사회적 지지와 관련 있었다. SNS에서의 자기표현은 직장인의 특성상 스트레스와 관련이 없었으나 SNS에서의 사회적 지지는 스트레스에 영향을 미치는 것을 확인하였다.

스마트워크 시스템 성공을 위한 사용자 만족의 영향요인에 대한 연구 : P그룹사를 중심으로

정창현,황찬규,홍순근,Jung, Chang Hyun,Hwang, Chan-Gyu,Hong, Soon-Geun 한국데이터전략학회 2013 Journal of information technology applications & m Vol.20 No.3

At the center of the repeating evolution of IT industry, there is mobile computing in the ubiquitous environment, and the increasing usage of smart phones contribute more to its growth. P corporation co-developed with Google and implemented smart work system called SWP for the purpose of 'communication and collaboration with co-workers' beyond the general goal of increasing productivity and comfort of employees. The primary objective of this study is to objectively analyze the influence of the effect factor from end user's perspective and user satisfaction on SWP usage, intention for continuous usage and individual performance. To accomplish this research purpose, this study established research models and hypothesis by collective review about information system success models, and tested the research hypotheses using the structural equation modeling technique by data collected from 320 SWP system users of P corporation. Variations for system satisfaction evaluation standard are 1)SW system usage 2) intention for continuous usage 3) individual performance, effect variations are 1) social factors 2) technical factors 3) combination of social and technical factors. Therefore, this study is about influence factors on success of SWP system and how the factors impact the outcome. The test results of this research model is summarized as follows. Firstly, system user's satisfaction had positive impact on all three; SWP system usage, intention for continuous usage and individual performance. Secondly, social factor 'SWP control system' and technical factor 'information quality' and 'system quality' had positive impact on SWP system user satisfaction, while social factor 'shared value' and technical factor 'service quality' did not have significant effect on user satisfaction. Shared value however, had interactive effect with 'information quality' and 'service quality'. This study is expected to contribute to spread of academic research on smart work system by suggesting a model that can show important factors for corporation while explaining the successful implementation of SWP and its continuous usage.