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제초제 paraquat와 bentazon의 세포독성과 3-methylcholanthrene의 독성경감효과
임요섭(Yo Sup Rim),서대호(Dae Ho Seo),한두석(Du Seok Han) 한국독성학회 2001 Toxicological Research Vol.17 No.2
This study were carried out to investigate cytotoxicity oj paraquat and bentazon that is scattering to farm products were essential for human diet and compensatory effects of 3-methylcholanthrene (3-MC) in vitro and in vivo. In vitro, The 5.0×10⁴cell/ml of NIH 3T3 fibroblast in each well of 24 multidish were cultured. After 24 hours, the cells were treated with solution of paraquat and bentazon (1, 25, 50, 100 J.1M respectively). After the NIH 3T3 Jib rob last of all groups were cultured in same condition for 48 hours, Sulfohordamin B Protein (SRB) assay were performed to evaluate the cytotoxicity of cell organelles. Paraquat and bentazon SRB50 were 1860.73 J.μM, 1913.38 J.μM respectively. In vivo, Sprague Dawley male rats divided into paraquat and bentazon only administered group and simultaneous application group of paraquat and bentazon and 3-MC. At 30 min. and I, 3, 6, 12,24,48 and 96 hrs. interval after each treatment, the animals were sacrificed by decapitation and kidney were immediately removed, immersed in fixatives, and processed with routine method for light microscopic study. Paraffin sections were stained with H-E, PAM, and PAS. Under the light microscope, atrophic change of renal corpuscles were frequently observed from 3 hrs after paraquat and bentazon treatment. The increase of the mesangium was apparent 12 hrs later after paraquat and bentazon treatment. Necrotic changes of the epithelium and loss of brush border of proximal tubules were most severe at 48 hrs after paraquat and bentazon treatment, respectively. In contrast there were no evidences of the toxic effects on renal tissues at 48hrs in paraquat and bentazon plus 3-MC treated groups.
몇 가지 식물추출물이 배양 NIH3T3 섬유모세포의 세포생존율과 세포부착률에 미치는 세포독성에 관한 연구
임요섭 ( Yo-sup Rim ),송원섭 ( Won-seob Song ),서영미 ( Young-mi Seo ),박승택 ( Seung-taeck Park ),김신무 ( Shin-moo Kim ) 대한임상검사과학회 2010 대한임상검사과학회지(KJCLS) Vol.42 No.3
This study was aimed to clerify the cytotoxicity of some plant extracts such as Hosta longissima HONDA (HL), Hemerocallis fulva var. Kwanso REGL (HFVK), Hemerocallis fulva L (HF), Macrocapium officinale NAKAI (MO) and Mentha canadensis var. piperascens HARA (MCVP), the cultured NIH3T3 fibroblasts were treated with 25, 50, 100, 150 and 200 μg/mL of five kinds of plant extracts for 48 hours, respectively. The cytotoxicity of plant extracts was measured by MTT and NR assays for the cell viability, and XTT assay for the cell adhesion activity. In this study, HL, MO and FHVK extracts showed the range of midtoxic-non toxic by the criteria of chemical cytotoxicity. While, the HF and MCVP extracts showed midtoxic. In the extract cytotoxicity, HL, MO and FHVK extracts showed non-toxic by the criteria of extract cytotoxicity. While, HF extract was determined as lower-toxic. In the responsive sensitivity of each plant extract on colorimetric assays, HF extract was sensitive to mitochondrial enzyme by MTT assay, lysosomal enzyme by NR assay and mitochondrial nucleus by XTT assay. While, MCVP extract was sensitive to mitochondrial enzyme by MTT assay and lysosomal enzyme by NR assay than other assays. While, HL, HFVK and MO extracts were most sensitive to NR assay. Cell culture is one of useful materials in the screening of cytotoxic and recovary effect on the putative chemical agents or plant extract. And also, colorimetric assay is regarded as very useful tools for quantitative measurement of cytotoxic effect on plant extracts in vitro.
환경오염원인 납의 신경독성에 대한 NMDA 수용체 길항제의 보호 효과
손영우,임요섭,서영미,Kim, Young-Wo,Rim, Yo-Sup,Seo, Young Mi 한국산업보건학회 2017 한국산업보건학회지 Vol.27 No.3
Objectives: This study was performed to evaluate the neurototoxicity of the environmental pollutant lead acetate(LA) and the protective effect of the D-2-amino-5-phosphonovaleric acid(APV), N-methyl-D-aspartate(NMDA) receptor antagonist on LA-induced cytotoxicity in cultured C6 glioma cells. Materials and Methods: For this study, cell viability in cultured C6 glioma cells was assessed by XTT assay and antioxidative effect, such as lactate dehydrogenase(LDH) activity, by LDH detection kit. Results: LA significantly decreased cell viability in a dose-dependent manner, and the XTT50 value was determined to be 33.3 uM of LA. The cytotoxicity of LA was deemed highly toxic according to Borenfreund and Puerner's toxic criteria. The vitamin E antioxidant significantly increased cell viability damaged by LA-induced cytotoxicity in these cultures. For the protective effect of APV on LA-induced cytotoxicity, APV significantly increased not only cell viability, but also inhibition of LDH activity. From these results, it is suggested that oxidative stress is involved in the neurotoxicity of LA, and APV effectively protected against LA-induced cytotoxicity via an antioxidative effect as an inhibotory activity of LDH. Conclusions: Natural resources like APV may be putative therapeutic agents for the toxic diminution of environmental pollutants such as LA correlated with oxidative stress.
유기게르마늄 수용액으로 재배된 콩나물의 생육특성과 게르마늄 흡수량
한성수(Seong Soo Han),임요섭(Yo Sup Rim),정재훈(Jea Hun Jeong) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.1
In order to cultivate the soybean sprout fortified with organogermanium, we observed growth characteristics and germanium content of soybean sprout watered with the aqueous organogermanium, Ge-132[bis(2-Carboxyethylgermanium sesquioxide). Soybean sprout did not show difference in growth when treated with different times and frequencies of organogermanium or different types of germanium. Growth of Orialtae soybean sprout was smaller in diameter of hypocotyledonary axis and longer in total length than that of Danyeob soybean sprout. Three hour`s soaking was better in growth than half an boar`s treatment. Germanium absorbed by soybean sprout was greater in content in Danyeob soybean sprout than in Orialtae soybean sprout and highest at 20 ppm in water. Prolonged period and increased frequency in treatments with organic germanium resulted in increase of germanium uptake. Germanium content in soybean sprout was greater with treatment of organic germanium than with inorganic germanium.
보문 : 염모제 성분인 카드뮴의 세포독성에 대한 시엽추출물의 영향
손영우 ( Young Woo Son ),임요섭 ( Yo Sup Rim ),유영월 ( Yeong Wol Yu ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2012 대한미용학회지 Vol.8 No.1
This study was carried out to investigate the cadmium (Cd)-induced cytotoxicity and the protective effect of persimmon leaves (PL) extract on the toxic effect induced by cadmium chloride (CdCl2) in cultured human skin fibroblasts (Detroit 551). In this study, CdCl2 showed the cytotoxicity by a significant decrease of the cell viability and cell adhesion activity (CAA). While, vitamin E as antioxidant protected CdCl2-induced cytotoxicity by the increase of CAA which was decreased by CdCl2. In the protective effect of PL extract against CdCl2-induced cytotoxicity, PL extract blocked CdCl2-induced cytotoxicity by the a significant increase of CAA as well as superoxide dismutase (SOD)-like activity and inhibitory activity of lipid peroxidation (LP). From these results, it is suggested that the cytotoxicity of CdCl2 is involved in oxidative stress, and PL extract showed the antioxidative effect by showing not only a significant increase of CAA but also SOD-like activity and inhibitory activity of LP. Conclusively, the physioactive components such as PL extract may be a putative beauty resources for the diminution or blocking the cytotoxicity or oxidative stress mediated by heavy metals or chemicals which are the materials of hair dyes or cosmetics.