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임상 검체에서 분리된 Bacillus cereus 의 성상, 장독소 생성 및 항균제 감수성
김신무 ( Shin Moo Kim ),김은철 ( Eun Cheol Kim ),소향아 ( Hyang Ah So ),이규식 ( Gyu Sik Lee ) 대한임상검사과학회 2005 대한임상검사과학회지(KJCLS) Vol.37 No.1
Biochemical characteristics, enterotoxin production and antimicrobial susceptibility were determined for 30 strains of Bacillus cereus isolated from stool specimens of diarrhea patients at an university hospital in Chulabuk-do province. Positive rate for VP reaction and citrate utilization were lower, (33 % and 40 % respectively) while the rates of acid production from mannitol, arabinose, and xylose were higher (17 %, 13 % and 3 % respectively) than those obtained by other investigators. The enterotoxin gene was detected in 18 of 30 isolates (60 %) by PCR, and the toxin was detected from all of the toxin gene-positive isolates by RPLA test. The agar dilution test showed that all isolates were resistant to penicillin G and 73 % were to cephalothin, but all were susceptible to ciprofloxacin, clindamycin, erythromycin, fusidic acid, gentamicin, rifampin, teracycline and vancomycin. We conclude that B. cereus isolates producing acid from mannitol, arabinose and xylose exist, that PCR can be used to detect enterotoxin genes rapidly and accurately, and that this organism is susceptible to various antimicrobial agents though not penicillin G and cephalothin.
혈액에서 Sucrose 양성 Vibrio vulnificus 분리 1예
김신무 ( Shin Moo Kim ),송계민 ( Kye Min Song ),김승아 ( Seung A Kim ),최수연 ( Su Youn Choi ),임효빈 ( Hyo Bin Im ),성치남 ( Chi Nam Seong ) 대한임상검사과학회 2004 대한임상검사과학회지(KJCLS) Vol.36 No.2
Vibrio vulnificus is a halophilic bacterium frequently involved in human infection of seafood-associated primary septicemia and primary wound infection, mostly in men with over 40-years of age with underlying liver disease. The primary septicemia, which is the most common form of V. vulnificus infection in Korea, is defined as a systemic illness presenting fever or hypotension with recovery of V. vulnificus from blood or tissue without the apparent primary focus of infection. V. vulnificus typically do not produce acid from sucrose, but a case of primary septisemia was found in a patient at Chonnam K hospital in 1993 from whose blood a sucrose-fermenting strain was isolated. The patient was a 62-year-old man, heavy drinker, with underlying liver disease. He consumed a raw seafood dish two days before onset of the present illness. His symptoms were tenderness and swelling on the right foot. He rapidly developed septicemia, resulting in sudden death. V. vulnificus was isolated from the venous blood culture of the patient. On subculture, the isolate formed yellow colonies on TCBS and produced acid from sucrose. Because of these characteristics, species identification was not achieved by the API 20E and was delayed. Other characteristics of the isolate were identical to those of typical V. vulnificus. The isolate was common serotype O4A and possession of V. vulnificus-specific cytolysin gene was detected by PCR. The isolate was susceptible to all the antimicrobial agents tested including tetracycline, but was intermediate to colistin. In conclusion, it is important that microbiologists be aware of the presence of sucrose-positive V. vulnificus when he or she identifies gram-negative bacilli, which is isolated from the blood of patients with a recent history of raw seafood dish consumption.
엄상검체에서 분리한 Vibrio parahaemolyticus의 생 물학적성상, 혈청형, toxR 유전자의 검출 및 시험관내 항균제 감수성
김신무 ( Shin Moo Kim ),송남경 ( Nam Kyung Song ),정재옥 ( Jae Ok Chung ),신상희 ( Sang Hee Shin ),소향아 ( Hyang Ah So ),이규식 ( Gyu Sik Lee ),정윤섭 ( Yun Sop Chong ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3
Vibrio parahaemolyticus is one of the most important food-bome pathogens in Korea, Japan, and other cOlllltries with long coast1ines. The aim of this study was to determine the biochemical characteristics, serovar, antimicrobial, susceptibi1ity and possession of toxR gene of the 46 V. parahaemolyticus isolates from c1inical specimens. Biochemical characteristics of the these were typical, but 28 % and 4 % of isolates showed negative to Simmon citrate and omithine decarboxylase test, respectively. Six kinds of serovars were detected in the isolates. Serovar 03:K6 were most prevalent i.e., 85%. Other serovar inc1uding 03:K9, 03:K55, 03:K56, 03:KUT and R were veη rare. Most of the isolates were resistant to ampici11in, carbenici11in and cefazolin, but none were resistant to most of the other antimicrobial agents including tetracyc1ine, which is the drug of choice for the treatment of Vibrio infection. V. parahaemolyticus toxR gene was detected from all of the 46 isolates. It is conc1uded that the biochemical characteristics of most of the V. parahaemolyticlα of isolated Wonkwang University Hospital are typical, that prevalent serovar are 03:K6, and that isolates with antimicrobial resistance with the exception ampicillin, carbenicillin 뻐d cefazolin are very rare. Also toxR gene detection by the PCR test can be used for rapid identification of the V. parahaemolyticus in the c1inical laboratory.
Sucrose 발효 및 비발효 Vibrio vulnificus 균주의 생물학적 및 유전학적 성상 비교
김신무(Shin-Moo Kim),소향아(Hyang-Ah So),송계민(Kye-Min Song),이영엽(Young-Youp Lee),임채원(Chae-Won Lim),이재형(Jae-Hyung Lee),소홍섭(Hong-Seob So),김진경(Jin-Kyung Kim),박래길(Rae-kil Park),박석돈(Seok-Don Park) 대한미생물학회 2006 Journal of Bacteriology and Virology Vol.36 No.4