꽃게 (Portunus trituberculatus) Hemocyanin의 정제 및 특성에 관하여

이강석,김상복,이정호,Lee, Kang-Suk,Kim, Sang-Bog,Lee, Jung-Ho 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.2

꽃게 (P. trituberculatus) hemolymph를 채취한 후 원심분리 및 DEAE-Sephadex A-50 column chromatgraphy를 행하여 hemocyanin을 정제하였다. 정제된 hemocyanin은 단일 polypeptide로 이루어져 있었으며 monomer의 분자량은 약 70,000으로 추정 되었다. 또한 꽃게 hemolymph 단백질중 hemocyanin의 함량은 46%, Cu는 단일 polypeptide 당 2 mole, 그리고 당 함량은 단백질 mg 당 $10\;{\mu}g$ 이었다. 한편 정제된 hemocyanin은 Ca등 2가 이온을 가하지 않고 pH 5.7~8.9인 상태에서 mono, di, tri, tetra, pentamer등 다양한 polymorphism을 나타내었다. The hemocyanin from the hemolymph of crab, Portunus trituberculatus which belong to crustacean was purified by a single step of ion-exchange column chromatography after dialysis and ultracentrifugation. The purified hemocyanin was revealed a high degree of polymorphism after analyzed it by Davis-polyacrylamide gel electrophoresis, the peroxidase activity, and copper staining method. On the other hand, the denatured hemocyanin was appeared to be homogeneous polypeptide by SDS-PAGE, and the approximate molecular weight was estimated to be 70,000. It was found that the purified hemocyanin was a glycoprotein in nature that contained 2 moles of copper/l mole of hemocyanin, and it was estimated that their contents in normal body fluid were approximately 46%.

꽃게 ( Portunus trituberculatus ) Hemocyanin 의 정제 및 특성에 관하여

이강석,김상복,이정호 ( Kang Suk Lee,Sang Bog Kim,Jung Ho Lee ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.2

The hemocyanin from the hemolymph of crab, Portunus trituberculatus which belong to crustacean was purified by a single step of ion-exchange column chromatography after dialysis and ultracentrifugation. The purified hemocyanin was revealed a high degree of polymorphism after analyzed it by Davis-polyacrylamide gel electrophoresis, the peroxidase activity, and copper staining method. On the other hand, the denatured hemocyanin was appeared to be homogeneous polypeptide by SDS-PAGE, and the approximate molecular weight was estimated to be 70,000. It was found that the purified hemocyanin was a glycoprotein in nature that contained 2 males of copper/l mole of hemocyanin, and it was estimated that their contents in normal body fluid were approximately 46%.

Studies on Histones from Sracoma 180 Ascites Tumor Cells. Biosynthesis and Phosphorylation

이강석,Lee, Kang-Suk 생화학분자생물학회 1971 한국생화학회지 Vol.4 No.2

1. Histone fractions, f1, f2b, f2a2, f3 and f2a1 were isolated from Sarcoma 180 ascites tumor cells and characterized by acrylamide disc electrophoresis, phosphorylation in vitro and amino acid analysis. 2. The rate of incorporation in vivo of L-Iysine-$^{14}C$ into different histone fractions isolated from tumor cells and tumor-bearing liver of mice has been determined for the study of biosynthesis. The fraction f2b from the tumor cells showed the highest incorporation rate, whereas the fraction f2a2 exhibited the lowest. No marked differences in the incorporation rate of the lysine-$^{14}C$ into the fractions f3, fl and f2a1 were observed 3. In the experiments with tumor-bearing mouse liver, the rate of lysine-$^{14}C$ into the histone fractions fl, f2a1, f2a2 and f3 was very similar to each other. On the other hand, the rate of incorporation of lysine-$^{14}C$ into the fraction f2b was approximately twice as high as that into other fractions. 4. The incorporation rate of lysine-$^{14}C$ into histone fractions isolated from the tumor cells was higher (approximately 1.5 times) than that into liver histone fractions. 5. Amino acid composition of the whole histone from the tumor cells is very similar to the published values for calf liver and kidney and rat liver. It is also similar to that for calf thymus except for the lower content of basic amino acids and lower ratio of basic residues. The whole histone fractions isolated from the tumor cells contained much less proline and methionine and much more glutamic acid, as compared with that isolated from others. Amino acid composition of the whole histone isolated from tumor-bearing liver of mice showed higher content of basic amino acids and lower content of acidic residues. 6. The relative amounts of incorporation in vitro of $^{32}P$ into different histone fractions from cells have been determined. The incorporation of $^{32}P$ into histone fractions f2b was the greatest, while almost no radioactivities were observed in the fractions f2a1 and f2a2. The incorporation of the radioisotopes into remaining fractions was variable from one fraction to another, the incorporation rate into the fraction fl being higher than that into the f3 fraction. 7. Evidence was obtained confirming the presence of phosphoserine in histone fractions f2b, f1 and f3 from cells. It was shown that a greater amount of $^{32}P$ was incorporated into the fraction f2b. being present in the form of phosphoserine in the proteins. 1. Sarcoma 180 ascites tumor cells에서 histone 분획 f1, f2b, f2a2, f3 및 f2a1을 각각 추출 정제하여 각 분획을 acrylamide disc electrophoresis. $^{32}P$를 사용하여 in vitro에서 phosphorylation 및 amino 산 조성을 관찰하여 이 물질들의 성상을 밝혔다. 2. 마우스의 복수암 세포 및 간에 lysine-$^{14}C$을 in vivo에서 incorporate 시켜 histone의 생합성을 각 분획별로 비교 관찰하였다. 복수암 세포 histone 에서는 lysine-$^{14}C$이 f2b 분획에 가장 많이 incorporate 된 반면 f2a2가 가장 적었다. 분획 f3, f1 및 f2a1에는 차이가 없이 비슷하였다. 3. 복수암이 이식된 마우스의 간 histone에 lysine-$^{14}C$의 incorporate 된 비율은 분획 f2b가 다른 분획 보다 2배 높았고 분획 f1, f2a1, f2a2 및 f3는 거의 비슷하였다. 4. Lysine-$^{14}C$의 incorporation은 복수암 histone 분획들이 간 histone 분획 보다 1.5 배나 더 많이 일어났다. 5. 복수암 세포 histone의 amino 산 조성은 이미 보고된 소의 간, 신장 및 흰쥐의 간 histone과 비슷하였다. 또 소 흉선 histone의 amino 산 조성에 비하면 염기성 amino 산 함량이 낮은 것과 산성 amino 산에 대한 염기성 amino 산의 비율이 낮은 점을 제외하고는 유사하였다. 복수암 세포의 총 histone을 기타 정상조직 histone과 비교하면 proline과 methionine이 대단히 적었고 glutamic acid의 함량이 더 많았다. 복수암을 이식한 마우스 간의 총 histone 의 amino 산 조성은 정상조직과 비교하면 염기성 amino 산 함량이 높았으며 산성 amino 산 함량이 낮었다. 6. 복수암 세포의 핵을 in vitro 에서 $^{32}P$와 함께 incubate 하고 histone 각 분획에 incorporate 되는 비율을 관찰하였다. Histone 분획 f2b에 가장 많이 $^{32}P$가 incorporate 된 반면 분획 f2a1 및 f2a2에는 거의 incorporate 가 일어나지 않았다. 기타 분획에 incorporate 되는 비율은 큰 차이를 나타냈다. 즉 분획 f1이 f3 보다 높은 방사능을 나타냈다. 7. Phosphoserine을 복수암 세포의 histone 분획 f2b, f1 및 f3에서 분리 확인하였으며 이런 점으로 미루어 분획 f2b 단백질 내에 phosphoserine의 형태로 많은 량의 $^{32}P$가 incorporate 됨을 알 수 있었다.

광합성 세균 Rhodobacter capsulata 에 의한 우라늄 흡착

이강석,김진규,김인규,박효국,이정호 ( Kang Suk Lee,Jin Kyu Kim,In Gyu Kim,Hyo Kook Park,Jeong Ho Lee ) 한국하천호수학회 1990 생태와 환경 Vol.23 No.4

Aqueous waste effluents from nuclear fuel processing operations may contain trace quantities of heavy metals such as uranium, Conventional treatment of waste waters may be ineffective or expensive when uranium concentrations in the waste water must be reduced down to 1 ㎍/㎖ or less. The ability of some microorganisms to uptake or adsorb dissolved heavy metals offers an alternative method for waste treatment. The adsorption of uranium by Rhodobacter capsulata DSM 1710, a strain of photosynthetic bacteria, was examined to identify factors that might affect a process for the removal of uranium from aqueous solution. The optimal pH for uranium adsorption was 4.5. Temperature had little or no effect on the process since more than 95% of dissoved uranium was adsorbed to the bacterial biomass within 10 minutes after mixing of cell biomass at 25, 30, and 40 ℃, all the same. Maximum capacity of uranium adsorption by R. capsulata DSM 1710 was equivalent to 1/4 of the dry cell mass (248 ㎍ U/㎎-dry biomass), the highest among the reported so far.

〈오징어 게임〉(2021)에 나타난 기억의 의미와 재해석: 마리안느 허쉬의 포스트메모리 이론을 중심으로

이강석(Kang-Suk Lee) 한국콘텐츠학회 2023 한국콘텐츠학회논문지 Vol.23 No.7

오늘날 사회문제에 대한 근원적 해결책은 공감의 형성이라는 인식이 확대되고 있으며, 다양한 영역의 논의에서 공감에 대한 중요성이 회자되고 있다. 본 연구에서는 황동혁 감독의 <오징어 게임>(2021)을 중심으로 하여 해당 작품에서 기억을 활용한 공감이 어떻게 표현되는지에 대해 허쉬의 포스트메모리 이론을 기반으로 하여 분석을 수행하였다. 연구결과, <오징어 게임>(2021)은 매체적 특성을 잘 살려내어 과거의 게임들이 제공하는 노스탤지어적 공포, 그로 인한 추억의 향수를 불러일으키는 장치를 마련하였다는 점, 극중 다양하게 펼쳐진 반전적 사건들이 모두 개개인들의 기억에 기반한 관람자의 공감을 이끌어 내는 장치가 되었다는 점, 마지막으로 누구나 당연한 사실로 치부할 수 있는 과거의 사실과 경험들이 주체에 따라 다르게 해석되고, 이 다른 해석들이 하나로 집대성되어 하나의 성공적인 공감을 이끌어 내었다는 점에 기억의 효과적인 활용성이 돋보인 작품이었다. 즉, 본 연구에서 주목한 <오징어 게임>(2021)의 사례는 이러한 공감이라는 가치를 기억의 전승구조를 통해 효과적으로 구현해 낸 장치를 마련하고 있었다. Today, the perception that the fundamental solution to social problems is the formation of empathy is expanding, and the importance of empathy is being talked about in discussions in various areas. In this situation, video contents using devices that induce empathy are gaining huge popularity, and in this study, based on Marianne Hirshs post-memory theory, how empathy using memory is expressed in the work, focusing on Hwang Dong-hyuks〈Squid Game〉(2021). As a result of the study,〈Squid Game〉(2021) made use of media characteristics to provide a device that evokes nostalgia for past games, various reversal events in the play have become devices that draw viewers sympathy based on individual memories, and finally, past facts and experiences that anyone can dismiss as obvious facts are interpreted differently. In other words, the case of 〈Squid Game〉(2021), which was noted in this study, was preparing a device that effectively realized the value of empathy through the transmission structure of memory.

영화 콘텐츠 관련 어플리케이션의 성공적 모델 제안: IS Success Model에 따른 소비자 태도와 관람의도를 중심으로

이강석(Kang-Suk Lee) 한국콘텐츠학회 2022 한국콘텐츠학회논문지 Vol.22 No.3

ICT 기술의 발전에 힘입어 다양한 모바일 어플리케이션이 속속들이 등장하고 이들이 개인의 삶 속에 깊이 파고든 시점, 영화산업의 새로운 성장판로를 모색하기 위하여 본 연구는 영화 콘텐츠 관련 어플리케이션의 성장 방안을 논의하였다. 실증연구를 통하여 영화 콘텐츠 관련 어플리케이션이 갖춰야 할 핵심요인을 탐색하기 위하여 본 연구에서는 정보시스템 성공 모델을 활용하여 각 품질요인이 영화 소비자의 영화에 대한 태도와 관람의도에 미치는 영향을 알아보았다. 연구 결과, 영화 콘텐츠 관련 어플리케이션의 품질요인 중 가장 중요한 요인은 정보 품질요인이었으며, 이어 서비스 품질이 차선으로 중요한 요인임이 도출되었다. 본 연구에서는 이와 같은 연구 결과를 바탕으로 국내 영화 산업의 발전을 위해 영화 콘텐츠 관련 어플리케이션들이 개선되어야 할 방안을 제시하여 새로운 유통 채널로서의 영화 콘텐츠 관련 어플리케이션의 방향성을 제안하였다. With the development of ICT technology, various mobile applications have appeared one after another, and they have penetrated deeply into the lives of individuals. In order to explore the key factors that movie content-related applications should have through empirical studies, this study used the information system success model to investigate the influence of each quality factor on movie consumers’ attitudes toward movies and their viewing intentions. As a result of the study, it was derived that the most important factor among the quality factors of movie content-related applications was the information quality factor, followed by the service quality being the next most important factor. In this study, based on the results of this study, the direction of application related to movie contents as a new distribution channel was proposed by suggesting a way to improve applications related to movie contents for the development of the domestic movie industry.

자연형 소하천의 호안공법별 식생분포 조사

이강석(Kang Suk Lee),박진기(Jin Ki Park),연규방(Gyu Bang Yeon),박종화(Jong Hwa Park) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.2

Riparian vegetation distribution patterns and diversity relative to various fluvial geomorphic channel patterns, stream bank stabilization methods, and stream flow processes are described and interpreted for selected nature-friendly small stream bank protection of Goesan, central Korea. [dong Stream Pilot Project, which began in May 2003 and finished in December 2003, was selected to develop effective methods which was nature-friendly stream bank protection. The project aim to maintain or increase stream bank stabilization ecosystem goods and services while protecting downstream and stream bank ecosystem. A number of protecting methods which were a Flight of fieldstone, Vegetation block, Green river block, Stone net, Green environment block, Eco friendly cobble, Vegetation mat and Gee-green cell and Firefly block were applied on the bank of ldong stream. The stream sites have been monitored about vegetation conditions each method in 2007. We selected six points to separately investigate in left and right bank. The main purpose of this study was to find out suitable methods and to improve stream restoration techniques for ecosystem. On the stream bank, H environment block method (9.7) was the highest average of vegetation coverage and Firefly block method (3.87) was the lowest average in applied methods.

Sarcoma 180 복수암의 Histone 의 생합성 및 인산화에 관한 연구

이강석 ( Kang Suk Lee ) 생화학분자생물학회 1971 BMB Reports Vol.4 No.2

1. Histone fractions, f1, f2b, f2a2, f3 and f2a1 were isolated from Sarcoma 180 ascites tumor cells and characterized by acrylamide disc electrophoresis, phosphorylation in vitro and amino acid analysis. 2. The rate of incorporation in vivo of L-lysine-^(14)C into different histone fractions isolated from tumor cells and tumor-bearing liver of mice has been determined for the study of biosynthesis. The fraction f2b from the tumor cells showed the highest incorporation rate, whereas the fraction f2a2 exhibited the lowest. No marked differences in the incorporation rate of the lysine-^(14) into the fractions f3, f1 and f2a1 were observed. 3. In the experiments with tumor-bearing mouse liver, the rate of lysine-^(14)C into the histone fractions f1, f2a1, f2a2 and f3 was very similar to each other. On the other hand, the rate of incorporation of lysine-^(14)C into the fraction f2b was approximately twice as high as that into other fractions. 4. The incorporation rate of lysine-^(14)C into histone fractions isolated from the tumor cells was higher (approximately 1.5 times) than that into liver histone fractions. 5. Amino acid composition of the whole histone from the tumor cells is very similar to the published values for calf liver and kidney and rat liver. It is also similar to that for calf thymus except for the lower content of basic amino acids and lower ratio of basic residues. The whole histone fractions isolated from the tumor cells contained much less proline and methionine and much more glutamic acid, as compared with that isolated from others. Amino acid composition of the whole histone isolated from tumor-bearing liver of mice showed higher content of basic amino acids and lower content of acidic residues. 6. The relative amounts of incorporation in vitro of ^(32)P into different histone fractions from cells have been determined. The incorporation of ^(32)P into histone fractions f2b was the greatest, while almost no radioactivities were observed in the fractions f2a1 and f2a2. The incorporation of the radioisotopes into remaining fractions was variable from one fraction to another, the incorporation rate into the fraction f1 being higher than that into the f3 fraction. 7. Evidence was obtained confirming the presence of phosphoserine in histone fractions f2b, f1 and f3 from cells. It was shown that a greater amount of ^(32)P was incorporated into the fraction f2b, being present in the form of phosphoserine in the proteins.

실감 콘텐츠를 활용한 영화산업의 가능성과 제언

이강석(Kang-Suk Lee) 한국디지털콘텐츠학회 2020 한국디지털콘텐츠학회논문지 Vol.21 No.9

Due to the development of information and communication technology, we live in a hyper-connected era, but at the same time, interactive communication through virtual technology has been in the spotlight in the untact culture. This study emphasizes the fact that, among various contents, virtual films form interactive communication in the context of this culture, and have rich potential as realistic contents in the future. As a result of examining the current problems, it was revealed that the lack of awareness and capacity of the people involved, the lack of support at the government level, and the legal system were not established. Accordingly, it was suggested that the producers need to accept and understand interactive communication and raise awareness of virtual films, while the government needs to examine the possibility of the film industry as realistic content and create a sound ecosystem for the future film industry. In the future, the film industry as immersive content is expected to become the most powerful and high-potential means for the public to experience real autonomy and various contents, and for producers to create creative works.

18. 마이크로파 전력측정의 표준화에 관한 연구

이강석(Lee, Kang Suk),정낙삼(Chung, Nak Sam) 대한전자공학회 1981 대한전자공학회 학술대회 Vol.1981 No.8