Identification of Growth Stimulatory Compound in the Mixed Culture of Lactobacillus helveticus YM-1 and Streptococcus thermophilus CH-1 in Milk

윤성식,유주현,Yoon, Sung-Sik,Yu, Ju-Hyun Korean Society of Food Science and Technology 1986 한국식품과학회지 Vol.18 No.6

환원탈지유배지에서 자란 Lactobacillus helveticus YM-1의 cell-free filtrate로부터 Streptococcus thermophilus CH-1의 생육촉진을 확인한 결과 이 물질은 분자량이 5,000정도의 peptide로 나타난다. 이 peptide 중에는 aspartic acid, Iysine, proline, leucine, valine, alanine, glutamic acid등의 amino산이 비교적 풍부하였으며, 그중에서 glutamic acid 함량이 가장 높았다. 또 이 Peptide가 생육 촉진효과 나타내는데 가장 중요한 역할을 담당하는 것은 glutamic acid와 Phenylalanine으로 판단되었다. A compound stimulatory to the growth of S. thermophilus CH-1 was isolated from the cell-free filtrate of L. helveticus YM-1 in milk medium. The stimulant was identified as a peptide with a molecular weight of approximately 5000 and exhibited positive ninhydrin reaction. Some kinds of amino acids confirmed as aspartic acid, alanine, valine, glutamic acid, lysine, proline, leucine were rich in the stimulatory peptide hydrolysate. Among them, glutamic acid was most abundant. Judging from bioautographic results, glutamic acid and phenylalanine were expected to exert an important role for the stimulation.

저온성 Pseudomonas sp. K101 로부터 protease 의 생산 및 정제

윤성식,소명환 ( Sung Sik Yoon,Myung Hwan So ) 한국낙농학회 1991 韓國酪農學會誌 Vol.13 No.1

노르웨이(Norway)의 유가공산업 소개 - 티나(TINE) 그룹을 중심으로 -

윤성식,Yoon, Sung-Sik 한국유가공학회 2008 Journal of Dairy Science and Biotechnology (JMSB) Vol.26 No.1

유당불내증(Lactose Intolerance)의 발생 원인과 경감 방안에 대한 고찰

윤성식,Yoon, Sung-Sik 한국유가공학회 2009 Journal of Dairy Science and Biotechnology (JMSB) Vol.27 No.2

Milk is called an almost complete food in terms of nutrition, especially for the younger generations because it contains a number of nutrients required for growth and development. Lactose intolerance is defined as a malabsorption of lactose in the intestine with some typical symptoms of abdominal pains and bloating, and occurred at 75% of global populations, which hampers milk consumption worldwide. Lacks of milk consumption in the underdeveloped countries frequently lead to many nutrients deficiencies, so that diseases including osteoporosis, hypertension, and colon cancer are more prevalent in the recent days. Lactose in foods needs to be hydrolyzed prior to intestinal absorption. The hydrolytic enzyme responsible for splitting lactose into its monomeric forms, glucose and galactose, is called as lactase or $\beta$-galactosidase. The former is primarily used as blood sugar and energy source and the latter used in glycolipid synthesis of brain tissues in infants. Lactose is clinically diagnosed with the breath hydrogen production test as well as intestinal biopsy. Reportedly, symptoms of lactose intolerance are widely prevalent at 25% of Europeans, 50 to 80% of Hispanics, South Indians, Africans, and Jews, almost 100% of Asians and native Americans. For the adults, phenotype of lactase persistence, which is able to hydrolyse lactose, is more common in the northern Europeans, but in the other area lactase non-persistence or adult-type hypolactasia is dominant. Genetic analysis on human lactase gene continued that lactase persistence was closely related to the err site of 1390 single nucleotide polymorphism from the 5'-end. To alleviate severity of lactose intolerance symptoms, some eating patterns including drinking milk a single cup or less, consumption along with other foods, whole milk rather than skimmed milk, and drink with live yogurt cultures, are highly recommended for the lactose maldigesters. Also, delay of gastric emptying is effective to avoid the symptoms from lactose intolerance. Frequency of lactose intolerance with conventional diagnosis is thought overestimated mainly because the subjects are exposed to too much lactose of 50 g rather than a single serving amount. Thus simple and accurate diagnostic method for lactose intolerance need to be established. It is thought that fermented milk products and low- or free lactose milks help improve currently stagnant milk consumption due to lactose intolerance which contributes to major barrier in milk marketing especially in Asian countries.

웅담약침액이 다종의 각막염 유발균에 미치는 항균력에 대한 실험

윤성식,서형식,Yoon, Seong-Sik,Seo, Hyung-Sik 대한약침학회 2010 Journal of pharmacopuncture Vol.13 No.1

Objectives : This experimental study was performed to investigate the effectiveness of Fel Ursi Pharmacopunture solution(FUPS) manufactured by using alcohol/water extraction method. To identify the use of it as eyedrops, antibacterial test on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, and Candida albicans was performed. Methods : After treatment FUPS on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) which cause Keratitis, we investigated anti-bacterial effects of FUPS on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans in terms of measuring MIC and size of inhibition zone respectively. Results : After FUPS was treated, significant changes of MIC on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum and Candida albicans were not observed at all. Conclusions : The present study suggests that FUPS doesn't have anti-bacterial effects on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum and Candida albicans which cause Keratitis. Perhap These results recommend that FUPS doesn't have anti-bacterial effects but have other mechanism which suppress inflammation.

안구건조증의 병인(病因)에 대한 문헌적 고찰

윤성식,서형식,Yoon, Seong-Sik,Seo, Hyung-Sik 대한한방안이비인후피부과학회 2009 한방안이비인후피부과학회지 Vol.22 No.3

Objective : The object of this literatual study is to investigate the etiology of dry eye syndrome Methods : We have selected data related to etiology of dry eye syndrome on the literature of Oriental Medicine. And we analyzed the study. Results : Treatment and etiology concerning dry eye syndrome derived diverse opinion according to medical era and practitioner. It was a good opportunity to speculate the etiology and similar symptoms of dry eye syndrome. Conclusions : Through literatual Study, we basically understood the etiology of dry eye syndrome. In the study of the etiology, it seems that further clinical studies on treatment of dry eye syndrome via oriental medicine.

Lactobacillus plantarum을 용균시키는 Bacteriophoge SC921의 분리 및 특성

윤성식,신영재,최학종,허송,오두환,Yoon, Sung-Sik,Shin, Young-Jae,Choi, Hak-Jong,Her, Song,Oh, Doo-Hwan 한국미생물·생명공학회 1997 한국미생물·생명공학회지 Vol.25 No.1

Among the lactic flora responsible for the development of acidity and characteristic flavor of Kimchi which is a traditional fermented Chiness cabbage. Homofermentative Lactobacillus plantarum is rod-shaped and to be known to ewert major role during later fermentation period. Once this strain establishes main flaora in the Kimchi fermentation process, it gives rise to excess acid production to reduce the taste and quality of Kimchi during storage. As a primary work to increase the keeping quality using virulent Lactobaillus plantarum bacteriophages, it was isolated sucessfully from collected Kimchi samples and their characteristics were studied. The new isolated phage, named SC 921, adsorbed to its host without Ca$^{2+}$, and nearly eliminated at 60$\circ $C of heat treatment for 5 min. This phages were atable at pH 4~ 10 but inactivated below pH 3.0 or pH 11.0 above. The latent period, rise period, and burst size of this phage was 100 min, 120 min, 31$\pm $2pfu/ml, respectively. Electron micrograph showed the phages particles were unusually oval feature of head (dia 80~ 120 nm) without contractile tail.

한국인 아동에서의 IL-1 유전자 다변성 연구

윤성식,정현주,김옥수,양규호,Yoon, Seong-Sik,Chung, Hyun-Ju,Kim, Ok-Su,Yang, Ku-Ho 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.2

The severe form of chronic periodontitis(CP) has been reported to be strongly associated with the presence of allele 2 of composite IL-1B(+3954) and IL-1A(+4845) genetic polymorphisms(genotype positive). However, other studies have reported conflicting findings. These might have resulted from differences in ethnic background and disease entities. The aim of this study was to determine the distribution of IL-1A(+4845), IL-1B(+3954), IL-1B(-511), and IL-1 RN(VNTR) genetic polymorphisms in children as a future Korean population. The study population consisted of 92 children from the Dept. of Pediatric Dentistry, Chonnam National University Hospital. Genomic DNA was obtained from buccal swab. The IL-1A(+4845), IL-1B(+3954), and IL-1B(-511) genes were genotyped by amplifying the polymorphic region using multiplex polymerase chain reaction(PCR), followed by restriction enzyme digestion and gel electrophoresis. IL-1 RN(VNTR) polymorphism were then evaluated by PCR amplification and fragment size analysis in agarose gel. The allele 2 frequency was 41.3%, 4.3%, 47.8%, and 9.9% for IL-1A(+4845), IL-1B(+3954), IL-1B(-511), and IL-1 RN respectively. The frequency of genotype with allele 2 carriage for IL-1A(+4845), IL-1B(+3954), IL-1B(-511), and IL-1 RN was 77.1%, 7.6%, 63.0%, and 15.2% respectively. The allele 2 frequency in IL-1B(+3954) was significantly higher in female than in male population(p<0.05). The negative association was shown between the presence of allele 2 in IL-1B(-511) and in IL-1B(+3954), and the carriage rate of IL-1B(+3954) allele 2 tended to lower in IL-1B(-511) allele 2(P=0.056). Only 7.3% of children carried the composite genotype of IL-1A(+4845) and IL-1B(+3954). These results suggest that the polymorphism of IL-1B(+3954) and the positive composite genotype was relatively rare in Korean population.

디지털영상보정기법을 이용한 콘크리트의 국부변형측정

윤성식(Yoon, Sungsik),박경수(Park, Kyoungsoo),권태현(Kwon, Tae-Hyun) 한국콘크리트학회 2013 한국콘크리트학회 학술대회 논문집 Vol.2013 No.10

린번천연가스 자동차용 산화촉매의 정화 및 열화특성

윤성식(Sungshik Yoon),정종우(Jongwoo Jung),이춘희(Choonhee Lee),최병철(Byungchul Choi) 한국자동차공학회 2002 한국자동차공학회 Symposium Vol.2002 No.11

This study was carried out to investigate the aging and the conversion caharacteristic of oxidation catalyst for natural gas vehicle with lean-burn system. The conversions of CH₄was observed over the various composition ratios of PMs(Precious metals) and washcoating methods. On the fresh catalvsts, Pd more affects on the activity at low temperature than other precious metals catalysts. The activity at low temperature was increased by increasing a mount of Pd. On the aged catalysts, the conversion efficiency of Pd-only catalyst with mono-layer washcoat was more decreased than the other catalysts for conversion on CH₄. It was observed that the thermal durability of Rh/Pd/Pt catalysts with double-layer washcoat was better than other mono-layer catalysts.