고구마 뿌리절편에서의 ${\beta}-glucuronidase$ 일시발현

신동일,박희성,Shin, Dong-Il,Park, Hee-Sung 한국응용생명화학회 2007 Applied Biological Chemistry (Appl Biol Chem) Vol.50 No.4

다채 (Brassica campestris var. narinosa) 유묘의 형질전환 및 일시발현의 정량적 분석

신동일,박희성,Shin Dong-Il,Park Hee-Sung 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.4

Brassica campestris var. narinosa는 새싹채소로 애용되는 식물로서 간단한 용기에서 손쉽게 재배할 수 있다. 본 연구에서는 vacuum-infiltration을 통한 Agrobacterium-mediated transformation에 의해서 B. campestris var. narinosa 싹에서의 GUS일시발현을 성공적으로 확인하였다. 발아전의 종자가 발아를 이미 시작한 종자보다 그 형질전환 효율이 높게 나타났다. 한편, hydrogen peroxide를 2일 생장시킨 싹에 처리한 후 형질전환 하였을 때 GUS발현이 증가되는 것을 관찰하였다. 간염항원유전자의 형질전환과 ELISA에 의한 항원단백질의 발현량 분석 시 hydrogen peroxide 처리 싹이 비처리 형질전환 싹에서 보다 2배 이상의 발현량이 측정되었다. Tah tasai chinese cabbage (Brassica campestris var. narinosa), a vegetable plant popularly consumed as several-days-old seedlings in oriental countries, can be easily cultivated using a simple appliance. We demonstrated that Agrobacterium-mediated transformation via vacuum infiltration (agroinfiltration) resulted in a successful transient GUS gene expression in tah tasai chinese cabbage seedlings. Pre-germinated seeds were found to be more susceptible to Agrobacterium infection than one-day-old or two-days-old seedlings. We also demonstrated that hydrogen peroxide (HPO) treatment increased GUS expression especially for two-days-old seedlings. In ELISA using seedlings transformed with hepatitis B surface antigen (HBsAg) DNA by agroinfiltration, HBsAg protein synthesis increased more than two folds by HPO treatment to two-days-old seedlings in comparison to the mock-treated pre-germinated seeds.

Sodium Hypochlorite Solution As a Chemical Wounding Agent for Improving Agrobacterium-mediated Chinese Cabbage Seed Transformation

신동일,박희성,Shin Dong-Il,Park Hee-Sung Korean Society of Life Science 2005 생명과학회지 Vol.15 No.6

배추 종자 및 유묘에 대하여 GUS발현 또는 hepatitis B surface antigen (HBsAg)발현 벡터를 지니는 Agrobacterium tumefaciens LBA4404 세포를 이용하여 진공침윤(agroinfiltration)에 의한 형질전환을 시도하였다. 특히 ELISA를 이용한 HBsAg발현의 정량적 분석에서 agroinfiltration방법은 형질전환효율이 매우 저조하게 나타났다. 그러나 차아염소산나트륨 용액을 발아 전 또는 발아 중인 배추종자에 처리한 후 agroinfiltration을 실시한 경우 형질전환 효율이 $2\~5$배 증가하였다. 따라서 차아염소산나트륨 등의 화학연마제에 의한 종자의 상처발생이 Agrobacterium의 감염을 용이하게 함으로써 배추유묘에서의 일시유전자발현을 증대시키는 것으로 제안되고있다. Chinese cabbage (Brassica campestris ssp. napus var. pekinensis Makino) seeds/seedlings were transformed via vacuum-infiltration with recombinant Agrobacterium tumefaciens LBA4404 cells. The agroinfiltration method was determined to be unsuccessful for Chinese cabbage transformation during the analysis of hepatitis B surface antigen expression by ELISA. However, treatment of sodium hypochlorite solution, prior to agroinfiltration, to pregerminated or germinating 1 day- or 2 days-old seeds was proven effectively to enhance transformation efficiency, suggesting that chemical wounding caused by sodium hypochlorite reaction might facilitate Agrobacterium infection and, therefore, transient gene expression in Chinese cabbage sprouts.

영어영문학 교육과정의 확장을 위한 스토리텔링 연구분야의 유의미성 탐구

신동일 ( Shin Dongil ),손정희 ( Sohn Jeonghee ) 대한영어영문학회 2014 영어영문학연구 Vol.40 No.2

The purpose of this study is to explore the meaningfulness of storytelling research based on English Language and Literature resources. It discusses the way such academic disciplines as English literature and linguistics need to be expanded or integrated in the areas of storytelling research. In the context of the ‘risk society’ and globally digitalized media, this paper discusses specific areas of research (such as digital storytelling, contents development, language identity, cultural studies, discourse analysis) related to the proposed program of English Language and Literature. The area of storytelling healing is elaborated to provide a touchstone case of how storytelling research can be embedded in English Language and Literature programs. Implication for further studies (e.g., actual curriculum implementation, professional development plans) is also discussed. (Chungang University)

워크플로우 관리 시스템의 설계 및 구현

신동일(Dong Il Shin),신동규(Dong Kyoo Shin) 한국정보처리학회 2000 정보처리학회논문지 Vol.7 No.5

Workflow means the automation of a business process, in which documents, information or tasks are transferred among participants for business action according to a set of procedural rules, and workflow management system is a system which defines, creates and manages the execution of workflow running one or more workflow engines. In this research, necessity of standardizing current workflow systems is recognized, and problems such as interoperability of current systems, dynamic adaptation to changing business environment and lacking of assessment, management and auditing of business processes are analyzed so that the design and implementation of a workflow system is focused on to offer a solution to the problems. The system is designed and implemented to change dynamically on Run-Time the processes definition defined on Build-Time, and interoperability is enabled by developing workflow engine and related modules based on the WfMC specifications and APIs.

유채유묘의 형질전환을 통한 일시발현시스템의 개발

신동일(Dong-Il Shin),박희성(Hee-Sung Park) 한국생물공학회 2006 KSBB Journal Vol.21 No.6

토익 수험자의 인식 연구 -비판적언어평가론 관점에서

신동일 ( Dong Il Shin ),김주연 ( Joo Yun Kim ) 21세기영어영문학회 2012 영어영문학21 Vol.25 No.2

The purpose of this study is to understand Korean test-takers` perceptions on TOEIC from critical language testing (Shohamy, 2001a). The interview data of this study were acquired from eleven college students who have learned TOEIC in everyday contexts. The results are found as follows: Firstly, test-takers saw TOEIC as an institutionalized test. They associated TOEIC with ``spec``, ``scale``, and ``gate keeper``. Secondly, TOEIC was perceived as a perpetuating tool of English status and successful career in Korea. Lastly, most participants took TOEIC-based English learning as building test-wiseness skills although a few expressed positive attitude toward TOEIC preparation. This study implies that TOEIC cannot be viewed as a neutral and positive measure of English proficiency, and that ``use-oriented testing`` needs to be considered in high-stake testing contexts, for the use of shared authority, collaboration and involvement of different stake holders. Implications for further research are also discussed.

온라인 게임 서버를 위한 부하 분산 미들웨어의 구현에 대한 연구

신동일(Dongil Shin),신동규(Dongkyoo Shin),김민수(Minsoo Kim),장재홍(Jaehong Jang),윤현숙(Hyunsook Yoon),이정훈(Junghoon Lee),한창완(Changwan Han) 한국정보과학회 2000 한국정보과학회 학술발표논문집 Vol.27 No.2Ⅰ

오늘날 온라인 게임 TCP망을 이용하여 동시에 수백에서 수천명이 접속하여 게임을 즐길 수 있는 클라이언트/서버 모델의 표준이지만 서버에 접속하는 클라이언트의 수가 증가함에 따라 나타나는 많은 문제점을 가지고 있다. 본 논문에서는 기존 네트웍 게임 엔진의 구조적 단점을 보완한 3-tier 방식을 적용한 분산 네트웍 게임 서버를 위한 부하 분산기의 연구 및 개발에 대하여 서술한다. 이 시스템은 클라이언트/서버 모델의 2-tier방식에서 오는 클라이언트 수 증가에 따른 네트웍 부하 가중에 대한 처리 문제와, 분산된 2-tier방식을 적용한 서버에서 각 서버간의 통신, 데이터 공유에 대한 문제 등을 다루고 이에 대한 해결책으로 3-tier방식을 적용하여 클라이언트/서버사이에 각 서버의 사용자나 이동되는 데이터를 분산하고 모니터링하는 부하 분산기 시스템을 적용하여 위와 같은 문제점을 해결하였다.

영어학습에 관한 TV 광고 다시 읽기: 생태주의 언어교육론으로부터

신동일 ( Dongil Shin ),심우진 ( Woojin Shim ) 대한영어영문학회 2016 영어영문학연구 Vol.42 No.2

This paper aims to criticize television advertisements on English language learning from the perspectives of ecological language learning. All the spoken texts of TV commercials on English language learning, which appeared between 2008 and 2015, were collected and transcribed for this study. The spoken texts were divided into the following three domains: 1) affective and cognitive variables in English language learning, 2) learning methodology and tools, and 3) English proficiency and success in life. Then the categorized texts were discussed through the alternative and critical discourse of ecological language learning(van Lier, 2004). In the TV advertisements, English language learning was often viewed through the lenses of modernist language ideology, where English language is still an input-output to be intrapersonally controlled, or an entity to be mastered for successful life. It was also implied that language learning is a matter of individual activity. From the ecological perspectives, however, the target language for learning is expanded into a constantly emergent set of linguistic possibilities. Language learning involves the multilayered nature of interaction and language use. It is hoped that the ecological viewpoints become a key foundation for alternative discourse in English language education, even in the media context. (Chungang University)

‘비판적’ 응용언어학 연구의 토대 탐색 : ‘실존의 미학’과 ‘비판적 존재론’으로부터

신동일(Shin, Dongil) 한국사회언어학회 2022 사회언어학 Vol.30 No.1

By reviewing different theoretical backgrounds behind knowledge, politics, and power of language in critical approaches to applied linguistics, this study introduces a newly constructed foundation for critical research, so called, Foucault’s ‘thoughts from outside.’ In order to understand Foucault’s experience of the outside, this study firstly explores the meanings of prohibition and transgression in literary texts, in which Foucault reflected the question of ‘limits.’ For example, research on Georges Batalille’s works provided an opportunity to consider the meaningfulness beyond the limitation of language. In the late works of governmentality and subjectification, Foucault also reinterpreted Kant’s concept of enlightenment and critique in the philosophy of actuality, defined as the ‘arts of existence’ or ‘critical ontology of ourselves.’ In this study, the critique as ethos is re-valued in an on-going inquiry and experimentation for the autonomous self-constitution of subject, which can be placed in the critical study of language, education, and society. Possible areas of research and L2 education (e.g., translingual practice, identity construction, narrative education) related to Foucault’s technological ways of living, as well as the limitations of this study, are also discussed.