국내 약학대학 학생들의 표준화 환자를 활용한 복약상담 교육 및 OSCE에 대한 인식

박태은,강민구,Park, Tae Eun,Kang, Minku 한국임상약학회 2022 한국임상약학회지 Vol.32 No.1

Background: Woosuk University College of Pharmacy delivered patient counseling education and administered Objective Structured Clinical Examination (OSCE) by utilizing standardized patients (SP) in Introductory Pharmacy Practice course. Surveys were conducted to examine students' perception of SP and its impact on experiential education. Methods: Students learned patient counseling by didactic lecture and role-play, followed by hands-on practice with SP. Then, patient counseling skills were assessed during OSCE by utilizing SP. The first survey was conducted immediately after OSCE, and the second survey after the completion of Pharmacy Practice Experiences (PPEs). Results: According to the surveys, majority of the students agreed that utilizing SP was effective in building confidence for conducting patient counseling, reflecting on strengths and weaknesses of communication skills, and preparing for PPEs. It was particularly more effective than role-play in simulating patient counseling in a real pharmacy and reflecting on oneself. Also, OSCE was an appropriate method to assess patient counseling skills and utilizing SP for this purpose was well-suited. Conclusion: Although it is very new in South Korea, other developed countries are already widely utilizing SP, and its importance and necessity have been proven by several studies. Therefore, Korean colleges of pharmacy should begin discussing how to utilize SP to improve students' communications skills and implementing OSCE to evaluate those skills.

축산식품이 국민보건영양에 미치는 영향: 오해와 진실

박태은,최윤재,Park, Tae-Eun,Choi, Yun-Jaie 한국축산식품학회 2014 축산식품과학과 산업 Vol.3 No.2

RF 스퍼터링으로 Si 기판위에 제작된 ZnO 박막에서 ZnO 버퍼층의 가스분위기 영향

박태은,조형균,공보현,홍순구,Park, Tae-Eun,Cho, Hyung-Koun,Kong, Bo-Hyun,Hong, Soon-Ku 한국전기전자재료학회 2005 전기전자재료학회논문지 Vol.18 No.7

The effects of gas atmosphere and in-situ thermal annealing in buffet layers on the characteristic of the ZnO grown by RF magnetron sputtering have been investigated. It was shown that the introduction of buffer layers grown at the gas atmospheres of the mixed $Ar/O_2$ and the in-situ thermal treatment of the ZnO buffer layer improved the structural and optical properties. In addition, the ZnO films on the buffer layer thermal-annealed at $N_2$ gas ambience showed the strong emission of the near band gap exciton with narrow linewidth by combining the high-temperature growth of the ZnO film.

표식유전자를 이용한 담배와 감자의 원형질체 융합

박태은,정해준,Park, Tae-Eun,Chung, Hae-Joun 배재대학교 자연과학연구소 1991 自然科學論文集 Vol.4 No.-

표식 유전자를 이용하여 체세포 잡종체를 선발하기 위한 연구의 일환으로 감자조직에는 T-DNA를 도입하여 식물호르몬 무첨가 배지에서도 생장가능한 형질전환체을 획득하고, 담배조직에는 NPT H gene을 도입하여 kanamycin에 대해서 저항성을 나타내는 형질전환체를 획득하여 각각의 특성구명과 원형질체를 유리하여 도입된 유전자 marker를 이용해서 융합을 시도한 바 그 결과는 다음과 같다. 1. 감자 괴경에서 Agrobacterium tumefaciens Ach5와 A. rhizogenes ATCC15834를 접종하여 crown gall tumor 및 hairy root를 유기하였으며 이러한 tumor조직은 식물 호르몬 무첨가 배지에서 생장이 가능하였다. 2. 감자에서 유기된 hairy root로부터 callus 형성은 2.4-D 2mg/1 첨가된 MS 배지에서 가장 양호하였으며 casein hydrolysate 1g/1가 첨가하면 유연 한 callus의 증식이 더욱 왕성하였다. 3. Activated charcoal이 0.5~2.0g/1 첨가된 배지에서는 crown gall tumor callus의 절단면이 갈변되는 것을 방지 할 수 있어 생존률을 높일 수 있었으나 hairy root에서는 갈변되어 고사되었다. 4. 2, 4-D 2mg/1와 casein hydrolysate 1g/1를 첨가한 배지에 hairy root callus를 현탁배의한 결과 양호한 많은 callus 덩어리들을 단시간에 얻을 수 있었다. 5. $Tri^-$parental mating으로 NPTII gene이 coding되어있는 binary vector인 pGA643을 wild type 및 disarmid된 Agrobacterium 내에 도입하여 Agrobacterium tumefaciens Ach5/pGA643, A.tumefaciens $A_4T$/pGA643, A. tumefaciens LBA4404/pGA643를 획득하였다. 이 세 개의 conjugant를 사용하여 0.7% agarose gel 상에서 pGA643을 확인하였다. 6. pGA643이 도입왼 Agrobacterium tumefaciens LBA4404와 담배 조직과 동시배양하여 kanamycin $100\mug$/ml 첨가된 배지에 생존하는 callus를 선발하였으며 동일배지에서 callus의 증식이 가능하였다. 7.형질전환된 담배 callus로부터 식물체 형성은 BA 2mg/1를 첨가한 배지에서 가능하였다. 8. 재분화된 담배의 엽조직은 kanamycin.이 $1000\mug$/ml 첨가된 MS 배지에서도 왕성히 callus가 유기되어, 이는 재분화체에서도 NPTII gene이 그대로 유지되고 있음을 확인할 수 있었다. 9. 담배의 정상 shoot와 형질전환된 shoot를 kanamycin이 $100\mug$/ml이 함유된 MS배지에 기내삽목한 결과, 정상 shoot는 발근이 되지 않고 황화 되었으나 형질전환된 shoot는 발근이되었으며 정상적으로 생장을 하였다. 10. 감자의 T-DNA가 도입된 현탁배양 callus는 cellulase 2%, macerozyme 2%, dricelase 1%에서 양호하게 유리되었다. 11. Osmoticum으로서 mannitol 농도 0.8M에서 담배와 감자의 두 조직 모두 원형질체유리가 가장 효과적이었다. 생존력은 T-DNA가 도입된 감자의 hairy root를 callus로 탈분화 시킨 후 현탁배양한 callus가 mannitol 0.5M에서 97%를 나타냈고, 그리고 NPTII gene이 도입된 담배의 엽조직은 mannitol 0.7M에서 94%로 최고를 타나냈다. 12. 원형질체 융합은 PEG solution 처리 15분 후부터 관찰되기 시작하여 20분에 완전히 융합되었고, 융합된 원형질체는 선발 marker인 호르몬 무첨가 및 kanamycin 첨가배지에서 배양 5일 후에 세포벽이 재생되었으며 4주일 후부터 colony들이 관찰되었다. These studies were carried out to select somatic hybrid using selectable marker genes of Nicotiana glauca transformed by NPTII gene and Solanum tuberosum transformed by T- DNA, and to study characteristics of transformant. The results are summarized as follows. 1. Crown gall tumors and hairy roots were formed on potato tuber disc infected by A. tumefaciens Ach5 and A. rhizogenes ATCC15834. These tumors and roots could be grown on the phytohormone free media. 2. Callus formation from hairy root was prompted on the medium containing 2, 4 D 2mg/I with casein hydrolysate lg/l. 3. The survival ratio of crown gall tumor callus derived from potato increased on the medium containing the activated charcoal 0. 5-2. 0mg/I because of the preventions on the other hand, hairy roots were necrosis on the same medium. 4. Callus derived from hairy root were excellently grown for a short time by suspension culture on liquid medium containing 2, 4-D 2mg/I and casein hydrolysate lg/l. 5. The binary vector pGA643 was mobilized from E. coli MC1000 into wild type Agrobacteriurn tumefaciens Ach5, A. tumefaciens $A_4T$ and disarmed A. tuniefaciens LBA4404 using a triparental mating method with E. ccli HB1O1/pRK2013. Transconjugants were obtained on the minimal media containing tetracycline and kanamycin. pGA643 vectors were confirmed by electrophoresis on 0.7% agarose gel. 6. Kanamycin resistant calli were selected on the media supplemented with 2, 4-D 0.5mg/1 and kanamycin $100\mug$/ml after co- cultivating with tobacco stem explants and A. tumefaciens LBA4404/pGA643, and selected calli propagated on the same medium. 7. The multiple shoots were regenerated from kanamycin resistant calli on the MS medium containing BA 2mg/l. 8. Leaf segments of transformed shoot were able to grow vigorusly on the medium supplemented with high concentration of kanamycin $1000\mug$/ml. 9. Kanamycin resistant shoots were rooting and elongated on medium containing kanamycin $100\mug$/ml, but normal shoot were not. 10. For the production of protoplast from potato calli transformed by T-DNA and mesophyll tissue transformed by NPTII gene, the former was isolated in the enzyme mixture of 2.0% celluase Onozuka R-10, 1.0% dricelase, 1.0% macerozyme. and 0.5M mannitol, the latter was isolated in the enzyme mixture 1.0% Celluase Onozuka R-10, 0.3% macerozyme, and 0.7M mannitol. 11. The optimal concentrationn of mannitol in the enzyme mixture for high protoplast yield was 0.8M at both transformed tobacco mesophyll and potato callus. The viabilities of protoplast were shown above 90%, respectively. 12. Both tobacco mesophyll and potato callus protoplasts were fused by using PEG solution. Cell walls were regenerated on hormone free media supplemented with kanamycin after 5 days, and colonies were observed after 4 weeks culture.

탄소강의 열간 연성에 미치는 저주기 피로 시기와 냉각속도의 영향

박태은 ( Tae Eun Park ),이운해 ( Un Hae Lee ),손광석 ( Kwang Suk Son ),이성근 ( Sung Keun Lee ),김인수 ( In Soo Kim ),임창희 ( Chang Hee Yim ),김동규 ( Dong Gyu Kim ) 대한금속·재료학회 2010 대한금속·재료학회지 Vol.48 No.3

The effects of cooling rate and onset time of fatigue test on hot ductility of plain carbon steels containing 0.06~0.8 wt.% carbon were investigated at various temperatures. The tensile tested specimen after fatigue strain during cooling showed higher hot ductility than the specimen fatigued and tensile-strained after cooling. With a decreasing cooling rate from solutionizing temperature to test temperature, hot ductility was increased in all temperature ranges, typically in the trough region, and the minimum point of ductility moved to a lower temperature. Also, the depth and width of the trough decreased with lowering the cooling rate.

논문 : 탄소강의 열간연성 거동에 미치는 산화의 영향

박태은 ( Tae Eun Park ),이운해 ( Un Hae Lee ),손광석 ( Kwang Suk Sohn ),이성근 ( Sung Keun Lee ),김인수 ( In Soo Kim ),임창희 ( Chang Hee Yim ),김동규 ( Dong Gyu Kim ) 대한금속재료학회 ( 구 대한금속학회 ) 2010 대한금속·재료학회지 Vol.48 No.5

The effects of oxidation behavior on the hot ductility of plain carbon steels were investigated at various temperatures in order to simulate the continuous casting process more precisely, in which the process undergoes in air atmosphere rather than Ar atmosphere. The high temperature oxidation behavior and scale morphology of the carbon steels exposed to the air and Ar atmosphere at various temperatures were also investigated in order to assess the mechanism of the RA value decreasing in an air atmosphere. The RA values obtained from the air atmosphere were marked below 45% by the test temperature, except for over 1000℃, with the RA values remaining in low values in both the low and high temperature region, at which the RA values generally recovered in the Ar atmosphere. The surface roughness of the specimen was developed by external and internal oxidation when the specimen was deformed in an air atmosphere at high temperature, with the result being the stress concentrated at the roughness of the specimen surface, resulting in low RA values. The hot ductility in the air atmosphere was found to be likely controlled by the oxidation rate instead of the microstructures corresponding to test temperatures.

Human BBB Model for Study for BBB Penetration of Drugs

Tae-Eun Park(박태은) 한국고분자학회 2020 한국고분자학회 학술대회 연구논문 초록집 Vol.45 No.2

합성절차에 따른 1차원 ZnO 나노구조의 형태조절과 특성평가

공보현,박태은,조형균,Kong, Bo-Hyun,Park, Tae-Eun,Cho, Hyung-Koun 한국전기전자재료학회 2006 전기전자재료학회논문지 Vol.19 No.1

The one-dimensional ZnO nanostructures prepared through thermal evaporation under various cooling down procedures by changing the flow rates of the carrier gas and the reactive gas were investigated. The nanorod structures were changed into the nanonail types with a broad head through the reduction of the flow rate of the carrier gas. The decrease of the reactive gas reduced the length of the nail heads due to the limited mass transport of reactive gas. The intensity ratio of the ultraviolet emission/green emission of photoluminescence was proportional to the length of the broad head showing a larger surface area. The vertically aligned nanostructures were grown along the [0001] direction of ZnO regardless of the aligned directions. The crystal direction of the nanostructures was determined by that of the initial ZnO crystal.

Aloe vera의 삽목 발근에 미치는 제요인에 관한 연구

정호선,침재성,박태은,Jeong, Ho-Seon,Chim, Jae-Seong,Park, Tae-Eun 배재대학교 자연과학연구소 1993 自然科學論文集 Vol.6 No.1

본 연구는 Aloe vera의 흡지번식에 있어서 auxin류의 처리와 몇 가지 재배요인이 발근에 미치는 영향을 실험한 바 다음과 같은 결과를 얻었다.1. 흡지의 크기와 근경의 유무에 따른 발근효과를 보면 단흡지(15-20cm)는 근 경을 제거한 것 보다 부착했을때 발근수가 많았고, 장흡지(25-30cm)는 근경을 제거했을때 근수 및 근건물중이 높은 경향을 나타냈다. 한편 단흡지는 근경이 부착되었을때 IBA 500mg/I처리에서 가장 높은 발근효과를 나타냈고, 장흡지는 근경을 제거했을때 IBA 1,000mg/I와 NAA 200mg/I처리에서 가장 촉진적이다.2. IBA, NAA, ethychlozate의 침지시간에 따른 무근경흡지의 발근양상을 보면 IBA와 NAA가 발근에 촉진적으로 작용했으나 NAA와 ethychlozate처리구에서는 약해를 받은 흔적이 나타나 aloe의 발근촉진에는 IBA가 가장 적합한 물질로 판단되었다. 또한 단흡지는 IBA 500mg/I에 10분 침지한 것이, 장흡지는 IBA 1,000mg/I에 10분 침지한 것이 근수, 최근장, 근중 및 발근율에서 가장 효과적 이었다. 그리고 흡지의 크기에 따른 발근효과는 장흡지가 더욱 촉진적이었고 균일한 발근양상을 나타냈다.3. 무근경장흡지의 근수와 근중은 IBA 1,000mg/I처리에 7일간 음건했을때 가장 높은 효과를 나타냈다. 그리고 IBA를 처리하지 않았을 때에는 음건이 길어수록 근장이 길어지는 경향을 보였다.4. Aloe흡지삽에 있어서 삽목용토에 따른 발근효과를 보면 인공용토 보다는 퇴비가 많이 함유된 재배토양에서 뿌리분화 및 뿌리생육이 가장 좋은 효과를 나타냈다.5. Aloe흡지삽의 발근은 투광율이 높을수록 양호한 경향을 보였고, 피복재료에 있어 서는 투명비닐 보다 흑색비닐이 발근을 더욱 촉진시켰다. Experiments were conducted to study the effect of auxins and cultural factors on rooting characteristics in offshoots cutting of Aloe vera. The result obtained were as follows;1. Short offshoots(15-20cm) with rhizome had more roots than that of the removed rhizome, while long offshoots(25-30cm) without rhizome had more roots and heavier root dry weight than short offshoots with rhizome. The highest rooting ability occured when IBA treated 500mg per liter in short offshoots with rhizome, whereas long offshoots without rhizome showed higher rooting ability at the level of 1,000mg/l of IBA and 200mg/I of NAA2. Among three kinds of anxin treated, both IBA and NAA showed better rooting ability than ethychlozate. Root number, root length and root dry weight were higher at 10 min. dipping treatment of IBA 500mg/I for short off-shoots without rhizome and 10 min. dipping treatment of IBA 1,000mg/l for long offshoots with rhizome. Rooting ability of long offshoots was higher than that of short offshoots.3. In addition to IBA 1,000mg/l, 7-day dryness under shading condition increased root number and dry weight of root in offshoots without rhizome. Control treatment lengthened root by increasing drying period under the shading condition.4. The plain soil with mamure had not only higher root dofferentiation but higher root growth of offshoots cutting than the artificial soil.5. Rooting ability in offshoots cutting had a tendency increasing with decrease in shading percentage to natural. Black color poly ethylene film mulching had better rooting ablility than transparency poly ethlene film mulching in offshoots cutting.

뇌 내 약물전달 시스템 개발을 위한 혈액 - 뇌 장벽 세포모델

최정원(Jeong-Won Choi),박태은(Tae-Eun Park) 한국고분자학회 2021 고분자 과학과 기술 Vol.32 No.3