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The Fine Functional Mapping of Penicillin G Acylase Gene
김영창,강현삼,오상진,송영환 한국유전학회 1982 Genes & Genomics Vol.4 No.2
Escherichia coli ATCC11105로부터 penicillin G acylase (PGA) 유전자를 plasmid pBR300에 cloning하여 얻은 pPAKS-1 및 그 유도체들에 있어서 PGA 유전자의 위치를 정확히 결정하기 위하여 우선 제한 효소 Eco RI, BamHI, Pst I, Sal I, Pvu II, Bgl I, Hind III, HinC II, Alu I, Kpn I, Hpa I, Xho I, Sau3A I 등을 이용하여 double digestion 또는 end-labelled DNA의 Partial digestion에 의해 제한 효소지도를 작성하였다. 이 제한효소지도를 기초로 하여 각종 제한 효소로 유발시킨 deletion mutant들의 제한효소절편과 효소역가를 상호 조사 비교함으로써 유전자의 위치를 결정할 수 있었기에 보고하는 바이다.
김영창,곽희철,김정한,문상원,Kim, Young-Chang,Gwak, Heui-Chul,Kim, Jung-Han,Moon, Sang-Won 대한족부족관절학회 2009 대한족부족관절학회지 Vol.13 No.2
Although avascular necrosis of the hallucal sesamoid has not been frequently addressed in the literature, it should be considered in the differential diagnosis of persistent forefoot pain. We experienced 3 cases of avascular necrosis of the hallucal sesamoid with sclerosis of the sesamoid bone on radiograghs and computed tomograghy images. T1 and T2-weighted MRI images in 2 patients showed low signal intensity in the sesamoid bone, which suggested osteonecrosis and confirmed by histology. We report 3 cases of avascular necrosis of sesamoid with a review of the literature.
인삼 종자의 개갑률 향상을 위한 적정 수확시기 및 GA<sub>3</sub> 처리 효과
김영창,김영배,박홍우,방경환,김장욱,조익현,김기홍,송범헌,김동휘,Kim, Young Chang,Kim, Young Bae,Park, Hong Woo,Bang, Kyong Hwan,Kim, Jang Uk,Jo, Ick Hyun,Kim, Kee Hong,Song, Beom Heon,Kim, Dong Hwi 한국약용작물학회 2014 韓國藥用作物學會誌 Vol.22 No.6
This study was performed to identify optimal harvesting time of ginseng seeds and to examine the effect of $GA_3$ treatment for improvement of seed stratification rate. Ginseng seeds harvested from Land race, Chunpoong and Yunpoong cultivar in July 20 were tested for stratification rate. It was shown that stratification rates of land race, Yunpoong and Chunpoong cultivar were 94.1%, 93.1%, and 82.6%, respectively. Seeds of Chunpoong cultivar harvested 10-15 days later showed a comparable stratification rate to that of Land race, indicating that late harvest of Chunpoong seeds is beneficial for the increase of stratification rate. The higher stratification rate was found in mature seeds (92.3%) than immature seeds (37.8%), both of which were harvested in July 20. Stratification rate of mature seeds harvested in July 15 was 87.5%, demonstrating optimal harvesting time of ginseng seeds with higher stratification rate is after mid-July. An exponential growth of endosperms of ginseng seeds was observed from early June to mid-June and then slow growth was observed. There was no obvious growth of embryos from fertilization to mid-August. After the this time, embryos quickly grew until late October. Thus, appropriate stratification control is essential during the period (from early September to late October) in order to optimize embryo growth and development. While no increase of stratification rate was observed in seeds treated with 50 ppm of $GA_3$, significant increases were observed in seeds treated with 100 ppm of $GA_3$. At this concentration of $GA_3$, the stratification rate of Land race, Chunpoong and Yunpoong cultivar was 95.0%, 95.3%, and 96.5%, respectively.