PDGF-BB와 IGF-I 혼합 투여가 비글견 인공 치근단 병소의 치유에 미치는 영향에 관한 연구

김미리,김민겸,윤수한,Kim, Mi-Ri,Kim, Min-Kyum,Yoon, Soo-Han 대한치과보존학회 2000 Restorative Dentistry & Endodontics Vol.25 No.1

It is difficult to treat the endodontic apical perforation successfully. In this study, we hypothesized that the application of PDGF-BB and IGF-I into periapical perforation site may accelerate periapical healing and lead to bone deposition. And the specificity of osteonectin in periapical healing was investigated. The experiments were performed on the upper and lower 51 premolar teeth of 4 beagle dogs. The pulp chamber of each tooth was opened and the dental plaque was inserted into the canal for developing the periapical lesion for 5 weeks. Then, the roots were artificially perforated at the apex with the number 4 profile of .06 taper. In each step, standard periapical radiographs were taken to compare the size of lesion each other. The radiographs were scanned and analyzed by image analysis system. The mean and standard deviation of periradicular radiolucency ratios were calculated in each group. ANOVA was used for comparison. 51 premolars were grouped into 3 groups; control group, calcium hydroxide-treated group and calcium hydroxide plus growth factors-treated group. In the control group, the apical perforations were not sealed and obturated with gutta-percha and ZOE sealer by lateral condensation technique. In the experimental groups, the apical perforation were sealed with calcium hydroxide and with/without $4{\mu}g$ of PDGF-BB & IGF-I in cellulose gel and obturated by lateral condensation technique. Fluorescent bone markers were used to measure new bone formation. Following 2, 4, 12 weeks after experiment the dogs were sacrificed and histologic sections were prepared. Each tooth block including periapical lesion was sectioned mesiodistally. One half of the sections were decalcified with 6% nitric acid and processed by standard paraffin embedding technique. The sections were stained by hematoxylin and eosin, and immunostained for osteonectin. Histomorphometrical measurement of neoformed bone was performed using a light microscope. And the other half of the sections were prepared by undecalcified preparation, and confocal laser scanning microscopic investigations were done.

슈미트의 경험 모듈을 중심으로 한 플렉서블 디스플레이 활용 방안 연구 - 패키지디자인을 중심으로 -

김미리,홍우정,김응화,Kim, Mi-Ri,Hong, U-Jeong,Kim, Eung-Hwa (사)한국포장협회 2022 包裝界 Vol.349 No.-

도로건설사업 시 지형 훼손 최소화를 위한 절·성토 높이 기준 연구

김미리,김수련,신지훈,성현찬,Kim, Mi-Ri,Kim, Su-Ryeon,Shin, Ji-Hoon,Sung, Hyun-Chan 한국조경학회 2014 韓國造景學會誌 Vol.42 No.1

본 연구의 목적은 경기도 도로건설사업의 환경영향평가서를 대상으로 도로 건설시 절 성토 높이와 비탈면 높이와의 관계를 분석하고, 도로 건설사업 시 지형적 훼손을 최소화할 수 있는 합리적인 비탈면 높이 기준을 제시하는 데에 있다. 이를 위해 도로건설사업 환경영향평가서 내 비탈면 높이와 관련된 전문가 의견이 제시된 44개의 사례를 검토하고, 그 중에서 절 성토고 높이에 관해 언급된 23개의 사례를 추출하여 분석에 활용하였다. 본 연구의 결과를 요약하면 다음과 같다. 환경영향평가서 상에 제시된 대부분의 절 성토 높이 및 비탈면 높이는 기준 높이인 20~30m(성토 높이), 그리고 10~15m(절토 높이)를 초과하고 있으며, 도로변의 절 성토 높이가 비탈면의 높이와 높은 상관관계가 있다는 것을 보여주고 있다. 그리고 회귀분석 결과에 따르면 각각의 절 성토 높이는 절토 높이의 경우 25.33~33.23m, 성토 높이의 경우 14.56~18.08m로 나타났으며, 이러한 높이 기준은 일반적인 높이 기준보다 높은 것으로 나타나, 향후 환경영향평가서 검토 시에 충분히 고려되어야할 사항으로 판단된다. 본 연구는 환경영향평가서에 제시된 절 성토 높이 및 비탈면 높이만을 고려하고 있어, 실제 제안된 높이가 지형 훼손을 최소화할 수 있는지에 대한 검증은 불가능하였으며, 따라서 본 연구에서 제시된 높이 기준을 바탕으로 비탈면 높이에 대한 친환경성 검증을 위한 심층적인 연구가 진행될 필요가 있다. The purpose of this study is to analyze the relationship between height of cutting and filling as well as the height of slope of roadsides. It also suggests the rational height of slope to minimize topographical damage in road construction. Hence, in this study, 44 cases of expert's opinions related to height of slope in Environmental Impact Assessment(EIA) for road construction projects are reviewed, and 23 cases of data related to height of cutting and filling in EIA for road construction projects are used for analyzing relationship between height of slopes and height of cutting or filling of roadside. The results are as follows; Most of heights of cutting, filling and slope in EIA for road construction are over the required standards 20 or 30m(in case of cutting) and 10 or 15m(in case of filling). It also shows that there is high-level correlation between height of cutting and filling and height of slope of roadside. According to regression analysis, it is suggested that the general standard of each heights of cutting and filling are 25.33~33.23m(in case of cutting) and 14.56~18.08m(in case of filling), but it should be considered in EIA review for road construction projects that these heights suggested in this study are over the required standards.

대두 리폭시게나제 효소와 아라키돈산의 반응중 자기불활성화의 요인에 관련된 중간체 생성물

김미리,석대은 ( Mee Ree Kim,Dai Eun Sok ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.4

Gradual decrease of soybean lipoxygenase activity was observed during the prior incubation with arachidonic acid, while preincubation with linoleic acid or 11,14,17-eicosatrienoic acid had no effect on lipoxygenase activity. Among the lipoxygenation products of arachidonic acid, only 15(S)-hydroperoxy eicosatetraenoic acid (15(S)-HPETE) inhibited lipoxygenase in a time-dependent manner. Either further oxygenation products of 15(S)-HPETE or non-enzymatic decomposition products of 15(S)-HPETE had no significant inhibitory effect. The observations that the inhibitory effect of 15(S)-HPETE was transient, and not affected by hydroxy radical scavangers support the assumption that the unstable lipid intermediates produced from further conversion of 15(S)-HPETE may be responsible for the irreversible inhibition of lipoxygenase. In support of the above assumption, 13(S)-hydroperoxy-6, 9, 11-octadecatrienoic acid, which can be further lipoxygenated was also found to inactivate lipoxygenase effectively. However, 13(S)-hydroperoxy-9,11-octadecadienoic acid or 13(S)-hydroperoxy-9,11,15-octadecatrienoic acid was not effective.

무우 Myrosinase의 정제 및 특성

김미리,이혜수,Kim, Mee-Ree,Rhee, Hei-Soo 한국식품과학회 1989 한국식품과학회지 Vol.21 No.1

무우의 myrosinase의 특성 규명을 위해 수행한 효소 정제는, 생 무우의 조효소액을 친화성 컬럼 (충진물 : Con A-Sepharose)에 흡착 시킨 후 ${\alpha}-methyl-D-mannoside$로 용출하였을 때 53%의 회수율과 16배의 정제도(S.P.A.=27,900 units/mg)를 나타내었다. 다음 단계로 gel permeation HPLC에 의해 정제한 결과, 약 50%의 최종 회수율과 22배의 정제도(S.P.A.=39,000 units/mg)를 나타내었으며, 전기 영동상 순도가 입증되었다. Gel permeation HPLC에 의해 측정한 분자량은 약 124,000 이었으며, sinigrin을 기질로 하여 측정한 Km, Vmax값은 각각 0.12mM, $40\;{\mu}moles/mg{\cdot}min$이었다. 작용 최적 pH는 6.5, 최적 온도는 $37^{\circ}C$ 이었고 중성용액(pH 6-7)에서는 상온에서 비교적 안정하였으나 산성용액 (pH 4 이하)에서는 불안정하여 효소활성도는 급격히 감소하였다. 효소의 활성화제인 아스코르브산에 의한 최대 활성화 농도는 0.6mM로 약 100배의 효소 활성도 증가효과를 나타내었으며 2mM이상에서는 효소 활성도가 저하되었다. 무우중 myrosinase의 분포는 껍질 부위에 평균 1,333 units/g, 속 부위에 평균 140 units/g으로 껍질 부위가 속 부위에 비해 10배 정도 많은 효소를 함유하고 있었다. The purification of myrosinase from radish roots was performed using Concannavalin A-Sepharose affinity chromatography and gel permeation HPLC, which gave a 22-fold-purification(S.P.A.=39,000 units/mg) with 50% recovery, SDS-polyacrylamide gel electrophoresis showed a single major band, suggestive of a relatively pure myrosinase, and the M.W. of the enzyme determined on gel permeation HPLC was ca. 124K. The enzyme showed on optimum pH of 6.5 and was stable at pH 6 to 7 at room temperature, but unstable below pH 4. The enzyme possessed an optimum temperature of $37^{\circ}C$, and gave a Vmax value of $40\;{\mu}moles/mg{\cdot}min$ and a Km value of 0.12mM for sinigrin. The purified myrosinase was activated maximally by 0.6mM of ascorbic acid, but somewhat inhibited by more than 2 mM ascorbic acid. The activities of myrosinase present in the peelings and the peeled radish amounted to approximately 1,333 units/g and 140 units/g weight, respectively and the peelings contained much more myrosinase activity than the peeled radish.

2016 연구과제 ③ - ➎ 기계설비건설업 경기지수 개발 연구

김미리,Kim, Mi-Ri 대한기계설비건설협회 2017 기계설비 Vol.321 No.-

의치용 레진치와 수복용 복합레진 간의 결합강도에 관한 연구

김미리,정창모,전영찬,임장섭,Kim, Mee-Lee,Jeong, Chang-Mo,Jeon, Young-Chan,Lim, Jang-Seop 대한치과보철학회 2002 대한치과보철학회지 Vol.40 No.2

This study investigated the shear bond strengths between abrasion-resistant denture teeth and composite resins according to surface treatments. Denture teeth for this study were Trubyte IPN teeth(Dentsply Inc., USA) with interpenetrating polymer network and Endura Posterio (Shofu Inc. Japan) of composite resin teeth, and restorative composite resins were Clearfil FII (Kuraray, Japan) of the self-cured composite resin and Z100(3M Dental Product, USA) of the light-cured composite resin. Five different surface treatments were evaluated: (1) $50{\mu}m\;A1_2O_3$ sandblasting: (2) #100 carbide paper; (3) chloroform; (4) retentive holes; and (5) no treatment. After surface treatments, denture teeth were examined by scanning electron microscopy(SEM), and the maximum shear bond strengths between abrasion-resistant denture teeth and composite resins were measured using Instron. The results were as follows; 1. IPN teeth treated with sandblasting had the highest shear bond strength, and Endura treated with sandblasting and carbide paper had significantly greater shear bond strength than with any other surface treatment. 2. Regardless or composite resins, the shear bond strength on Endura was greater than on IPN teeth. 3. Regardless of denture teeth, the shear bond strength of Clearfil FII was greater han of Z100. 4. In appearance of SEM, IPN teeth treated with sandblasting showed generalized roughness on the all of surface, however, carbide paper treatment resulted in partly rough. Endura treated with sandblasting and carbide paper showed similar surface characteristics. Wetting denture teeth surface with chloroform removed the debris and created a particle-free and smooth surface.

2016 연구과제 ② - ➍ 기계설비건설업체의 부도예측모형 연구(II)

김미리,Kim, Mi-Ri 대한기계설비건설협회 2017 기계설비 Vol.320 No.-

호수 생태계에서 살아있는 세균을 측정하기 위한 qDVC 방법의 적용

김미리,서은영,최승익,안태석,Kim, Mi-Ree,Seo, Eun-Young,Choi, Seung-Ik,Ahn, Tae-Seok 한국미생물학회 2006 미생물학회지 Vol.42 No.3

호소수내의 '살아있는 세균'을 측정하기 위하여 quantitative direct viable count (qDVC) 방법을 적용하였다. qDVC방법에 적용되는 최적 glycine 농도는 2%였으며, '살아있는 세균'을 계수하는데 있어서 평판계수법, CTC법 보다는 qDVC 방법이 보다 효과적이라는 것을 확인하였다. qDVC방법으로 '살아있는 세균'을 측정한 결과 다른 두 방법보다 $2.4{\sim}6.0$배 높은 값이었다. 또한 qDVC방법은 '살아있는 세균'을 죽은 세포 또는 휴면세포와 쉽게 구별할 수 있었다. For measuring the viable cells in lakes, quantitative direct viable count (qDVC) method is applied. In the qDVC process, the final concentration of glycine is fixed as 2%. For confirming the effectiveness of qDVC for enumerating the viable cells, the viable bacterial numbers were measured by plate count, CTC reduction method and qDVC method at 5 different lakes. Among these 3 methods, the bacterial numbers by qDVC is $2.4{\sim}6.0$ times higher than those by the other 2 methods. And by the qDVC method, the viable cells were easily discriminated from dead or dormant cells.

Protein Profiles of Major Korean Rice Cultivars

김미리,Chuanha Wei,Oh Yun Kwon,Xi-Wen Liu,Hyoung Chin Kim,윤원기,Hwan Mook Kim 한국식품영양과학회 2007 Preventive Nutrition and Food Science Vol.12 No.2

The protein profiles among Korean rice cultivars were assessed by total protein determination, solubility frac- tionation, SDS-PAGE analysis and scanning densitometry. In the extraction of protein, the SDS/urea system at a neutral pH was more efficient than that at alkaline pH. The determination of total protein showed that the protein content was similar among cultivars, ranging from 87.9 to 92.7 mg/g dry weight. Additionally, the water/NaCl-soluble protein fraction, containing 14~16 kDa albumin and 22 kDa globulin α-globulin, was also similar among cultivars, with a range of 9.94 to 11.98 mg/g dry weight. The SDS-PAGE/densitometry of total protein showed that there was no discernable difference in proteins of higher molecular weights among various cultivars, whereas the amount of lower molecular weight proteins (14~16 kDa) is somewhat variable among cultivars. Furthermore, SDS-PAGE analysis of water/NaCl-soluble and propanol-soluble fractions indicates that there is a discernible change in the content of albumin, globulin or prolamin among cultivars. Thus, the PAGE/densitometry method, preceded by solubility fractionation, is useful for examining differences in protein profiles of rice cultivars.