미역과 쇠미역 추출물의 항산화 및 간 보호 효과

김기안,오태환,천상호 (사)한국해양생명과학회 2021 한국해양생명과학회지 Vol.6 No.2

본 연구는 미역(Undaria pinnatifida) 추출물과 쇠미역(Costaria costata) 추출물의 항산화 활성 및 HepG2 세포의 알코올로 인한 산화 손상에 대한 보호 효과를 연구하였다. 미역과 쇠미역 추출물의 총 폴리페놀과 플라보노이드 함량은 70% 에탄올 추출물에서 가장 높았다. 또한 미역과 쇠미역 70% 에탄올 추출물의 DPPH (IC50 0.33±0.21, 0.48±0.47 mg/ml), ABTS (IC50 0.34±0.30, 0.47±0.17 mg/ml) 라디칼 소거 활성이 열수 추출물 및 10% 에탄올 추출물 보다 높았다. 추출물의 간 보호 효과를 확인하기 위하여 HepG2 세포에 알코올 산화 스트레스를 유발하여 MTT 분석을 이용하여 세포 생존력을 측정하였다. 미역 및 쇠미역 열수 추출물은 알코올 처리군(73.95%) 대비 세포 생존율은 각각 89.91~97.63% 및 84.99~90.54%로 농도 의존적으로 증가시켰다. 본 연구는 미역과 쇠미역 추출물이 간 보호 및 항산화 효과를 나타냄을 확인하였고 알코올 산화 손상에 대한 간 보호 소재로 활용될 수 있을 것으로 사료된다.

Comparison of the content of bioactive substances and antioxidative activity between conventionally and organically cultivated brown rice (Oryza sativa L.)

김기안,조정용,이유석,이형재,정항연,이유건,문제학 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.3

The content of bioactive substances and antioxidative activity in conventionally grown brown rice (CGBR) and organically grown brown rice (OGBR) were compared. Minerals (mg/100 g) such as magnesium (OGBR, 168.59±2.62; CGBR, 121.43±2.22), copper (OGBR, 0.50±0.06; CGBR, 0.41±0.05), and manganese (OGBR, 4.70±0.04; CGBR, 2.49±0.02) were higher in OGBR than in CGBR (p<0.05). In addition, levels of (μg/100 g) vitamins B2 (OGBR, 27.22±2.56; CGBR, 22.12±2.24) and B6 (OGBR, 46.32±2.66; CGBR, 39.91±3.32) were higher in OGBR than in CGBR (p<0.05). The contents (mg/100 g) of β-sitosterol (OGBR, 27.40±2.79; CGBR, 24.75±1.06), total phenolic (OGBR, 6.72±0.02; CGBR, 6.64±0.02), and ferulic acid (OGBR, 1.75±0.45; CGBR, 1.11±0.14) as well as the antioxidative activity (OGBR, 53.09±1.90%; CGBR, 48.29±3.38%) evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay were higher in OGBR than in CGBR, although no significant differences between samples were observed. In comparison to the control group, brown rice samples significantly inhibited cholesteryl ester hydroperoxide formation in rat plasma subjected to copper ion-induced lipid peroxidation. The inhibitory effect of OGBR was higher than that of CGBR. These results indicate that OGBR showed higher levels of bioactive substances and enhanced antioxidative activity than CGBR, although the differences were not statistically significant.

관행 및 유기재배 현미의 생리활성 성분의 함량 및 항산화활성 비교

김기안,조정용,이유석,이형재,정항연,이유건,문제학,Kim, Gee An,Cho, Jeong-Yong,Lee, You Seok,Lee, Hyoung Jae,Jeong, Hang Yeon,Lee, Yu Geon,Moon, Jae-Hak The Korean Society of Food Preservation 2017 한국식품저장유통학회지 Vol.17 No.5

The content of bioactive substances and antioxidative activity in conventionally grown brown rice (CGBR) and organically grown brown rice (OGBR) were compared. Minerals (mg/100 g) such as magnesium (OGBR, $168.59{\pm}2.62$; CGBR, $121.43{\pm}2.22$), copper (OGBR, $0.50{\pm}0.06$; CGBR, $0.41{\pm}0.05$), and manganese (OGBR, $4.70{\pm}0.04$; CGBR, $2.49{\pm}0.02$) were higher in OGBR than in CGBR (p<0.05). In addition, levels of (${\mu}g/100g$) vitamins B2 (OGBR, $27.22{\pm}2.56$; CGBR, $22.12{\pm}2.24$) and B6 (OGBR, $46.32{\pm}2.66$; CGBR, $39.91{\pm}3.32$) were higher in OGBR than in CGBR (p<0.05). The contents (mg/100 g) of ${\beta}$-sitosterol (OGBR, $27.40{\pm}2.79$; CGBR, $24.75{\pm}1.06$), total phenolic (OGBR, $6.72{\pm}0.02$; CGBR, $6.64{\pm}0.02$), and ferulic acid (OGBR, $1.75{\pm}0.45$; CGBR, $1.11{\pm}0.14$) as well as the antioxidative activity (OGBR, $53.09{\pm}1.90%$; CGBR, $48.29{\pm}3.38%$) evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay were higher in OGBR than in CGBR, although no significant differences between samples were observed. In comparison to the control group, brown rice samples significantly inhibited cholesteryl ester hydroperoxide formation in rat plasma subjected to copper ion-induced lipid peroxidation. The inhibitory effect of OGBR was higher than that of CGBR. These results indicate that OGBR showed higher levels of bioactive substances and enhanced antioxidative activity than CGBR, although the differences were not statistically significant.

초음파 처리에 의한 검정라즈베리 부위별 에탄올 추출물의 산화방지 활성

김기안(Ki An Kim),권지웅(Ji Wung Kwon),김용석(Yong-Suk Kim),박필재(Pill Jae Park),채규서(Kyu Seo Chae) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.4

본 연구는 검정라즈베리 부산물인 줄기, 잎 및 씨 초음파 추출물의 산화방지 활성에 미치는 영향을 살펴보고, 산화방지 활성을 가지는 식품 및 의약품의 개발 가능성을 알아보기 위하여 수행되었다. 검정라즈베리 부위별을 물 및 25, 50, 75, 100% 에탄올을 추출용매로 초음파 추출하여 실험을 진행한 결과 총 폴리페놀 함량의 경우 검정라즈베리 줄기의 25, 50% 에탄올 추출물이 각각 348.21±5.40, 343.39±5.94 ㎎/g으로 가장 높은 함량을 보였고, 잎은 50% 에탄올 추출물이 161.06±3.57 ㎎/g, 씨의 경우는 75% 에탄올 추출물이 266.29±2.51 ㎎/g으로 나타나 줄기의 총 폴리페놀 함량이 가장 높음을 확인하였다. 페놀성 화합물 10종의 분석 결과 모든 시료에서 갈산과 엘라그산이 가장 높은 함량의 화합물로 분석되었고, 쿼르세틴, 페룰산, 쿠마르산, 카페산, 루틴, 루테올린은 모든 추출물에서 분석되었다. 총 플라보노이드 함량을 측정한 결과에서는 추출용매간에 함량에 차이가 나타나는 것을 확인하였는데, 줄기는 25% 에탄올 추출물이 76.02±4.23 ㎎/mL로 가장 높은 함량을 보였고, 잎의 경우는 50% 에탄올 추출물이 82.28±0.54 ㎎/mL로 가장 높았으며, 씨의 경우는 75% 에탄올 추출물이 82.40±2.49 ㎎/mL로 가장 높게 나타났다. 산화방지 활성 검증을 위한 DPPH 및 ABTS 라디칼 소거활성의 EC<SUB>50</SUB> 값을 비교한 결과 총 폴리페놀 함량이 가장 높았던 줄기의 활성이 가장 높았으며, 씨>잎 순으로 높은 라디칼 소거활성을 보였고, 추출용매 조건에 따라서 활성의 차이가 크게 나타났다. 또한 환원력을 측정한 결과에서도 줄기>씨>잎 순으로 높은 환원력을 보였고, 검정라즈베리 부산물인 줄기, 잎 및 씨 모두 높은 수준의 산화방지 활성을 가지는 것으로 확인되었으며, 추출조건으로는 줄기의 경우 25, 50% 에탄올, 잎의 경우 50% 에탄올, 씨의 경우 75% 에탄올 용매 조건에서 산화방지 활성이 가장 우수한 것으로 나타났다. 결과적으로 검정라즈베리 부산물인 줄기, 잎 및 씨 모두 산화방지 활성이 우수한 천연소재로서의 이용이 가능할 것으로 판단되었고, 특히 줄기의 경우 산화방지 활성이 매우 뛰어나 건강기능 식품 및 의약품과 같은 산업적 이용 가치가 높을 것으로 판단된다. This study was carried out to investigate the antioxidant effects of different parts (stems, leaves, and seeds) of the black raspberry for utilization as food materials. Different parts of the black raspberry were subjected to extraction via ultra-sonication extraction methods using water and ethanol at various concentrations (25, 50, 75, and 100%). Antioxidant capability of the extracts were determined by amounts of phenolic compounds, with flavonoid contents, radical scavenging activity, and reducing power. Irrespectively of ethanol concentration, extracts of stem showed the highest total phenolic compounds and antioxidant activities among different parts of black raspberry. The total phenolic compounds extracted from the black raspberry stem using 25 and 50% ethanol showed 348.21±5.40 and 343.39±5.94 ㎎/g, respectively. Fifty percent ethanol extracts of the black raspberry stem showed the highest DPPH (EC50 value: 60.89 ㎍/mL) and ABTS radical scavenging activities (EC50 value: 82.57 ㎍/mL). Further, 25% ethanol extacts of the black raspberry stem (0.263±0.004) was found to have the highest reducing power. The highest antioxidant activity of black raspberry stem indicates that black raspberry stem may be useful source for functional food.

韓國企業의 對中國 投資 戰略에 관한 實證硏究

朴基岸;金明律 경희대학교 산업관계연구소 1993 産硏論叢 Vol.18 No.-

[초록없음]

On the Normal Varieties

Lee, Kee-An,So, Kwang-Ho,Kim, Yang-Kon 全北大學校 1983 論文集 Vol.25 No.-

X를 代數的 多樣體라 하자, 旣約인 代數的 多樣體 X는 그 座標環A(X)가 整閉이면 正規多樣體라 하며 代數的 多樣體의 모든 스타크(stalk)가 UFD이면 局所 팩토리알(factorial) 多樣體라 한다. 또한 正規多樣體 X上의 素因子 Γ는 codim(Γ)=1을 갖는 X의 閉部分多樣體로 定義한다. 本 論文에서는 任意의 局所팩토리알 多樣體가 多樣體가 되고 局所팩토리알 多樣體 X上의 모든 素因子Γ는 局所主多樣體임을 證明하였다.

Electron Beam Melting 공정으로 제조된 Ti-Al계 금속간 화합물의 미세조직 및 기계적 특성

이기안(K. A. Lee),김영균(Y. K. Kim),윤성준(S. J. Youn),김성웅(S. W. Kim),홍재근(J. Hong) 한국소성가공학회 2019 한국소성가공학회 학술대회 논문집 Vol.2019 No.5

Mg+A2Ca 첨가 ADC12 (Al-Si-Cu) 합금의 미세조직, 인장 및 고주기 피로 특성

김영균,김민종,김세광,윤영옥,이기안 한국소성∙가공학회 2017 소성가공 : 한국소성가공학회지 Vol.26 No.5

This study investigated the microstructure, tensile strength, and high cycle fatigue properties of ADC12 aluminum alloys with different Mg+Al2Ca contents manufactured using die casting process. Microstructural observation identified the presence of α-Al, eutectic Si, Al2Cu, and Fe-intermetallic phases. The increase of Mg+Al2Ca content resulted in finer pore size and decreased pore distribution. Room temperature tensile strength tests were conducted at strain rate of 110-3/sec. For 0.6%Mg ADC12, measured UTS, YS, and El were 305.2MPa, 157.0MPa, and 2.7%, respectively. For 0.8%Mg ADC12, measured UTS, YS, and El were 311.2 MPa, 159.4 MPa, and 2.4%, respectively. Therefore, 0.8% ADC12 alloy had higher strength and slightly decreased elongation compared to 0.6% Mg ADC12. High cycle fatigue tests revealed that 0.6% Mg ADC12 alloy had a fatigue limit of 150 MPa while 0.8% Mg ADC12 had a fatigue limit of 160MPa. It was confirmed that Mg+Al2Ca added ADC12 alloy achieved finer, spherical eutectic Si particles, and Al2Cu phases with greater mechanical and fatigue properties since size and distribution of pores and shrinkage cavities decreased as Mg+Al2Ca content increased.

Mg+Al₂Ca 첨가 ADC12 (Al-Si-Cu) 합금의 미세조직, 인장 및 고주기 피로 특성

김영균,김민종,김세광,윤영옥,이기안,Kim, Y.K.,Kim, M.J.,Kim, Shae K.,Yoon, Y.O.,Lee, K.A. 한국소성가공학회 2017 소성가공 : 한국소성가공학회지 Vol.26 No.5

This study investigated the microstructure, tensile strength, and high cycle fatigue properties of ADC12 aluminum alloys with different $Mg+Al_2Ca$ contents manufactured using die casting process. Microstructural observation identified the presence of ${\alpha}-Al$, eutectic Si, $Al_2Cu$, and Fe-intermetallic phases. The increase of $Mg+Al_2Ca$ content resulted in finer pore size and decreased pore distribution. Room temperature tensile strength tests were conducted at strain rate of $1{\times}10^{-3}/sec$. For 0.6%Mg ADC12, measured UTS, YS, and El were 305.2MPa, 157.0MPa, and 2.7%, respectively. For 0.8%Mg ADC12, measured UTS, YS, and El were 311.2 MPa, 159.4 MPa, and 2.4%, respectively. Therefore, 0.8% ADC12 alloy had higher strength and slightly decreased elongation compared to 0.6% Mg ADC12. High cycle fatigue tests revealed that 0.6% Mg ADC12 alloy had a fatigue limit of 150 MPa while 0.8% Mg ADC12 had a fatigue limit of 160MPa. It was confirmed that $Mg+Al_2Ca$ added ADC12 alloy achieved finer, spherical eutectic Si particles, and $Al_2Cu$ phases with greater mechanical and fatigue properties since size and distribution of pores and shrinkage cavities decreased as $Mg+Al_2Ca$ content increased.

Pectinase를 이용한 복분자, 오디, 아로니아 과즙의 항산화 활성

손락호,채규서,김기안,권지웅,박재숙,조하니 한국약용작물학회 2014 한국약용작물학술대회 발표집 Vol.2014 No.10