[현상과인식 노우트] 아일랜드와 경제주의에서 벗어나기

공경희 한국인문사회과학회 2000 현상과 인식 Vol.23 No.4

왜곡된 대학 성문화, 이제는 바로잡을 때

공경희,Gong, Gyeong-Hui 한국대학교육협의회 2000 大學敎育 Vol.107 No.-

섬망사정도구를 사용하는 중환자실 간호사의 섬망인식, 섬망사정의 장애요인 및 섬망사정 수행의 중요도

공경희,하이경,강인순,Gong, Kyung-Hee,Ha, Yi-Kyung,Gang, In-Soon 한국중환자간호학회 2015 중환자간호학회지 Vol.8 No.2

Purpose: This study was conducted to identify the perception, barriers, and importance of delirium assessment of intensive care unit nurses utilizing a tool for delirium assessment. Methods: The subjects were 150 intensive care unit nurses who routinely use the CAM-ICU to screen delirium. Data were collected using self-report questionnaires and analyzed using descriptive statistics and ranking. Results: Most intensive care unit nurses had experience of education about delirium and delirium assessment, and had assessed over once in a shift. However, a small number of nurses recognized utilizing their assessment result and the effect on higher mortality and underdiagnosed and preventable problem. The first rank of barriers was being too busy to assess patients and the second was difficulty of interpreting intubated patients. The importance of delirium assessment was considered lower than assessing catheter placement and level of pain. Conclusion: The study identified intensive care unit nurses' use of a delirium assessment tool, and the perception, barriers, and importance of delirium assessment. Furthermore, it is necessary to develop education programs to improve the early recognition of delirium by intensive care unit nurses.

White spot syndrome virus (WSSV)에 대한 단클론 항체 생산 및 특성 분석

공경희,김춘섭,김도형,김위식 한국어병학회 2022 한국어병학회지 Vol.35 No.2

White spot syndrome virus (WSSV) is a prevalent and virulent pathogen affecting cultured whiteleg shrimp (Litopenaeus vannamei) in Korea. In this study, seven monoclonal antibodies (mAbs) (10A12, 16C3, 17G4, 21G5, 22C4, 23B6 and 24G6) were produced by using purified WSSV. The reactivity of these mAbs was analysed by Western blot (WB), indirect immunofluorescence (IIF), and lateral flow immunochromatographic assay (LFIA). WB analysis demonstrated that three mAbs (17G4, 22C4, and 23B6) reacted specifically to VP28 with an approximate molecular weight of 24 kDa, mAb 16C3 reacted with approximately 17 kDa. IIF analysis demonstrated specific fluorescence signals on gill tissues of WSSV-infected shrimp, with five mAbs (10A12, 16C3, 22C4, 23B6, and 24G6), pleopods from WSSV-infected shrimp were used for LFIA, where, two mAbs (21G5 and 22C4) exhibited positive reaction. In conclusion, it can be inferred that the mAbs usage and specificity depends on the nature of assay used for diagnosis.

자음건비탕가오수유(滋陰健脾湯加吳茱萸) 추출물이 흰쥐의 뇌혈류역학에 미치는 영향

공경희,이은경,이기승,정현우,정명수,Kong, Kyunghee,Lee, Eunkyoung,Lee, Giseung,Jeong, Hyunwoo,Chong, Myongsoo 대한예방한의학회 2015 대한예방한의학회지 Vol.19 No.3

Objective : Jaeumgeonbitang have been used in Korean medicine for many centuries as a therapuetic agent of vertigo. JAE was extract of Jaeumgeonbitang adding Evodiae Fructus. The effects of JAE on the cerebral blood flow and blood pressure is not known. This study was designed to investigate the effects of JAE on the ischemic crebral injuries. Method : We performed to investigate effects of JAE on the changes of regional cerebral blood flow(rCBF) and mean arterial blood pressure (MABP) in normal and ischemic rats, and further to determine the mechanism and cytokines production ($IL-1{\beta}$, $TNF-{\alpha}$, IL-10, $TGF-{\beta}$) of JAE. Results : In normal rats, JAE significantly increased rCBF and significantly decreased MABP in a dose-dependent manner. This result suggested that JAE significantly increased rCBF by dilating pial arterial diameter. Increase of JAE-induced rCBF was significantly inhibited by the pretreatment with indomethacin (1 mg/kg, i.p.), an inhibitor of cyclooxygenase, and was significantly inhibited by methylene blue ($10{\mu}g/kg$, i.p.), an inhibitor of guanylate cyclase. Decrease of JAE-induced MABP was significantly increased by the pretreatment with indomethacin (1 mg/kg, i.p.), an inhibitor of cyclooxygenase. So, these results suggested that the mechanism of JAE was mediated by cyclooxygenase. In ischemic rat, the rCBF was significantly and stably increased by JAE (10 mg/kg, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in Control group. In cytokine production of serum by drawing from femoral arterial blood at 1 hr after reperfusion, Sample group (JAE 10 mg/kg treated group) was significantly decreased $IL-1{\beta}$ and $TNF-{\alpha}$ production compared with Control group. In cytokine production of serum by drawing from femoral arterial blood at 1 hr after reperfusion, Sample group was significantly increased IL-10 production compared with Control group. Conclusion : These results suggested that JAE was significantly and stably increased regional cerebral blood flow by inhibited $IL-1{\beta}$ and $TNF-{\alpha}$ production, and increased IL-10 production.

VHS (viral hemorrhagic septicemia)의 원인병원체인 VHSV (genotype IVa)에 대한 단클론 항체 개발

공경희 ( Kyoung-hui Kong ),오명주 ( Myung-joo Oh ),장민석 ( Min-seok Jang ),김춘섭 ( Choon-sup Kim ),김위식 ( Wi-sik Kim ) 한국어병학회 2019 한국어병학회지 Vol.32 No.2

본 연구에서는 넙치(Paralichthys olivaceus)로부터 분리한 바이러스성출혈성패혈증바이러스(viral hemorrhagic septicemia virus, VHSV, genotype IVa)에 대한 단클론 항체(monoclonal antibody, MAb)를 개발하였다. VHSV에 대한 항체를 생산하는 총 5개의 hybridoma clone을 생산하였다. 4개의 MAbs(2C10, 18H4, 23H6, 30B7)는 glycoprotein을 인식하였고, MAb 15E10은 nucleocapsid protein을 인식하였다. 5개의 MAbs는 western blot 상에서 VHSV에 감염된 세포와 넙치시료에 반응하였으나, 정상 세포와 넙치시료에는 반응하지 않았다. 또한 ELISA상에서 VHSV에만 반응하였고 6종의 어류바이러스(IHNV, HIRRV, SVCV, IPNV, MABV, NNV)에는 반응하지 않았다. 이상의 결과, 본 연구에서 제작된 MAbs는 VHSV에만 특이적으로 반응하는 것이 확인되어 VHSV를 검사하는데 사용될 수 있을것으로 사료된다. We developed and subsequently characterized mouse antibodies (MAbs) against viral hemorrhagic septicemia virus (VHSV, genotype IVa), the causative agent of VHS. Five hybridoma clones secreting MAbs against VHSV were established. The MAbs recognized the glycoprotein (MAbs 2C10, 18H4, 23H6, and 30B7) and nucleocapsid protein (15E10) of VHSV by western blot analysis. All five MAbs reacted with VHSV-infected cells and tissue homogenates of VHSV-infected olive flounder (Paralich­thys olivaceus) by western blot analysis. Whereas, no reactivity was observed in normal cells and tissue homogenates of normal olive flounder. Moreover, these MAbs reacted with VHSV, but did not react with other fish viruses (infectious hematopoietic necrosis virus, hirame rhabdovirus, spring viraemia of carp virus, infectious pancreatic necrosis virus, marine birnavirus, and nervous necrosis virus) by enzyme linked immunosorbent assay (ELISA). These results indicate that the MAbs are specific to VHSV and can be of value in VHSV detection.

Marine birnavirus (MABV)에 대한 단클론 항체 생산

공경희 ( Kyoung-hui Kong ),오명주 ( Myung-joo Oh ),김위식 ( Wi-sik Kim ) 한국어병학회 2020 한국어병학회지 Vol.33 No.2

We developed and subsequently characterized mouse monoclonal antibodies (mAbs) against marine birnavirus (MABV). Eight hybridoma clones secreting mAbs against MABV were established. All eight mAbs (8G6, 11C3, 15E3, 17H6, 32A6, 35A7, 38B5, and 47E3) were reacted with viral protein 3 of MABV in MABV-infected CHSE-214, whereas, no reactivity was observed in normal CHSE-214 by western blot analysis. Moreover, these eight mAbs were strongly reacted with MABV, and no cross-reactivity has been observed against other five fish viruses (hirame rhabdovirus, infectious hematopoietic necrosis virus, nervous necrosis virus, spring viraemia of carp virus, and viral hemorrhagic septicemia virus), although five mAb (11C3, 15E3, 17H6, 32A6, and 38B5) reacted with both MABV and infectious pancreatic necrosis virus by enzyme linked immunosorbent assay (ELISA). These results indicate that the mAbs can be of value in MABV detection.

韓國의 都市化 : 第二部 解放後 南韓의 都市化 Part 2. South Korean Urbanization, 1945-1960

共慶姬 慶北大學校 1963 論文集 Vol.7 No.1

This is Part 2 of the study "Urbanization in Korea". Following Part 1, which covers the period 1917-1845 under Japanese control, Part 2 deals with South Korean Urbanization since the liberation of the country in 1945 with emphasis upon the analysis of the process and effects of urbanization. In spite of (1) the withdrawal of the Japanese, who were mainly urban dwellers and held the overwhelming economic power in Korea Cities, (2) the fatal economic blow in its loss of industry, mineral resourcs and electric power caused by the division of the couutry, and (3) the destruction of urban areas by the Korean War, the process of urban growth in South Korea continued. Some of the characteristics revealed through the analysis of the process of urbanization in South Korea during the period 1845-1860 are as follows: 1. The trial urban population of South Korea hag grown from 2,831,926 to 6,999,844 or from 14,6% to 28% of tole national population during thin period. Growth during the period is estimated to be 147% (10.5% a year) in contrast to the national increase of 27% and the rural increase of 8.8%. 2. The number of cities increased from 14 to 27 during the same period. The greatest number of dities belonged to the middle class of cities (50,000 to 100,000 pesons). The largest increase in the number of cities occured in this middle class. 3. Compared to the growth of the other size groups, that of the largest cities is distinct. the ratio of the population of the largest cities to the total urban population increased from 79% to 81.6%, while the ratio for the middle clams cities and the small class cities decreased from 19.3% to 17.7% and front 1.6% to 0.7%, respectively, during the period. The population increase of the five largest cities was 50.7% of the total population increase of South Korea during the period. During the Korean War period (1949-55) this rate reached 76.9%. It was 22% during the period 1925-1944. 4. The rate of population growth in all cities existing in 1946 was over 50% for talc period. Cities showing the slowest growth were the port cities on the West Coast. The cities with the fastest growth were the refugee cities during the War, and these showed an increase of over 200%. 5. The following periodic trends can be identified during the period. A. Despite the confusion in the cities right after the liberation of the country, the growth of urban population during the period 1946-1949 was 22.7% in contrast to the national increase of 4.2% and rural increase of 1.1%. The available statistics showed that 93% of tole growth was due to the social increase in Seoul during the period May 1944-Apri1 1947. The periodic reasons for the rapid urban growth seem to be: (1) The repatriation of Koreans from foreign countries and the influr of refugees from North korea; (2) the political and social instability in the rural areas: and (3) the expected increase of opport unities for Koreans in the Cities. The decline of western port cities due to the cutting off of trade with Japen and China on the one hand, and the growth of the inla‥nd cities on the other hand, were the outstanding features of this period. B. The dastruction and demage of urban area by the korean War was phenomenal. In the case of the capital city, Seoul, 9% of human lives, about one third of the total houses, 44% of the industrial establishments (factories with over 70% destruction), 14% of national roads, 93% of local roads and 54.5% of the bridges were damaged. Many other urban facilities were also severely damaged. However the loss of lives and the destruction of cities by the Korean Ware did not change the imbalance between the continuously growing urban population and the economic base which support this population. The growth rate of urban population during the Korean War period reached 51.9%, which is the peak for the period after world war 11. The rise and fall of the population of cities during the war reflects the damage by the war and the advance and retreat of the population accompaying the tides of the war. The urban occupational structure in 1955 showed that the population of Korean cities, especially that of Seoul, were to some degree spcialized in urban occupations. C. The growth of urban population continued with a growth rate of over 24.5% during the latest period 1955-1960, which showed the fellowing features. (1) conspicuous population growth of she largest cities (population growth of the five largest cities was 38.5% of that of South Korea) (2)The slight growth of the population of the middle class cities(growth rate was about 10%). Most cities of this class ware subjected to less damage and absorbed over population during the Korean War period. (3) The increasing intensity of the dominance of the capital city Seoul (Seoul absorbed about 10% of the South Korean total population and 35% of the total urban population). The two biggest urban problems caused by this accelerated growth of urban population are 1. the econmic poverty of the cities and 2. Confusion of the urban area due to the failure to expand urban facilities. 1. Taking the example of Seoul which has overwhelming economic power in the nations economic life, some of (he evidences of urban poverty of the cities are as follows: A. 70% of the total of 504,716 households have been remitted their taxes. B. The structure of occupational population shows that, of the total number of employees, only 8.07%, are in secondary industry while 75.97% are in tertiary industry Of the latter 68% are the fatty marchants. This urban poverty is one of the sources of socioeconomic problems. C. 23%, of the tonal population are unemployed. This is a 7% decrease compared to that in 1937 (The ratio of unemployment to total urban population of South Korea is 25.8%). 2. Urban areas can not provide minimum facilities such as housing, water supply, sewage disposal, electricity, transportation and social and cultural establishments. Present urban planning is limited to only the reinforcement of already existing built up areas, roads, and sewage age systems. Some examples of the lack of the urban facilities are analysed. In view of the decree of industrial devlopment which is the urban ecomonic base, the urban population of South korea has reached its saturation point. But the movement of population concentulaion to the cities has been continuing steadily and rapidly despite the many political and socio-economic changes after World War 11 and will continue In the near future. even if there is not any adequate industrial development. As the counter-measures to be taken for the over population concentration which has arisen and has created numerous problems. the following are suggested. 1. Stabilize rural life. because the main cause of Korean urbanization is the rasult of rural poverty which pushes tremendous rural populations to the cities rather than the result of an urban pull due to urban industrial development. 2. develope urban indusrty to keep pace with the level of urbanization reached today. This is most urgent and fundmental. 3. Project economic and social stabilization pokily by comprehensive economic development. 4. develop local urban centers for specific functions. 5. establish a general policy of decentralization. 6. establish long-term urba planning to expand urban facilities.

Infectious hematopoietic necrosis virus (IHNV)에 대한 단클론 항체 생산

공경희 ( Kyoung-hui Kong ),오명주 ( Myung-joo Oh ),김춘섭 ( Choon-sup Kim ),김위식 ( Wi-sik Kim ) 한국어병학회 2023 한국어병학회지 Vol.36 No.2

Infectious hematopoietic necrosis virus (IHNV) is s significant viral pathogen affecting cultured rainbow trout (Oncorhynchus mykiss) in Korea. In this study, five monoclonal antibodies (mAbs) (IHNV-1, 2, 3, 4, and 5) were produced using purified IHNV. Reactivities of these mAbs were analyzed by western blot (WB), enzyme-linked immunosorbent assay (ELISA), and indirect fluorescent antibody test (IFAT). These mAbs recognized glycoprotein (69 kDa, IHNV-1), nucleocapsid protein (39 kDa, IHNV-3, 4, and 5), or phosphoprotein (27 kDa, IHNV-2) of IHNV by WB analysis. ELISA results indicated that these five mAbs were specific to IHNV without showing any cross-reactivity against other fish viruses (hirame rhabdovirus, infectious pancreatic necrosis virus, and viral hemorrhagic septicemia virus). IFAT demonstrated specific fluorescence signals of IHNV-infected epithelioma papulosum cyprini (EPC) cells, whereas no reactivity of normal EPC cells was observed. These mAbs can be very useful for immuno-diagnosis of IHNV infection.

흰다리새우(Litopenaeus vannamei )에서 분리된 WSSV의 전장유전체 분석

이아름,공경희,김휘진,오명주,김도형,김종오,김위식,Lee, A-reum,Kong, Kyoung-Hui,Kim, Hwi-Jin,Oh, Myung-Joo,Kim, Do-Hyung,Kim, Jong-Oh,Kim, Wi-Sik 한국어병학회 2022 한국어병학회지 Vol.35 No.1

The full genome sequence of a Korean white spot syndrome virus (WSSV, isolate: WSSV-GoC18) is presented here. We obtained a total of 12,320,554 reads with 291,172 bases, 170 gene, and 170 coding DNA sequence, which were assembled in 1 contig. Phylogenetic analysis revealed that the WSSV-GoC18 was closely related to Chinese isolate (WSSV-PC) and distinctly different with previously reported a Korean isolate (WSSV K-LV1). The complete genome sequence of WSSV isolates will be of great help in molecular epidemiological studies, contributing to molecular diagnosis and disease prevention in shrimp aquaculture.