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김화중,김홍성,임재현,조은경,송창화,박정규 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1
Identification and characterization of mycobacterial antigens are critical for evaluation of their role in diagnosis, vaccination, and pathogenesis of mycobacterial diseases. An attempt has been made to immunologic characterize the 55 kDa protein antigen of Mycobacterium tuberculosis H37Rv which attracted our interest because it is present in high concentration in 50-80% ammonium sulfate fraction of the culture filtrate. Two monoclonal antibodies (MAbs) directed to 55 kDa antigen were produced. MAbs MT55-1 and MT55-2 reacted with a single 55 kDa protein band. On examination of degree for cross-reactivity with other mycobacterial species by enzymelinked immunosorbent assay (ELISA) and immunoblotting, these antibodies reacted strongly with M. tuberculosis and M. bovis BCG, and reacted weakly with M. marinum and M. smegmatis. To investigated the subcellular distribution of MAbs defined epitopes in the 55 kDa antigen within the mycobacterium, we isolated three major subcellular factions of M. tuberculosis, namely, cell wall, cytoplasmic membrane, and cytosol, by a simple fractionation procedure. MAb MT55-1 reactive cpitope was found in the cytosol when tested by immunoblotting. A sandwich ELISA was initially developed for detecting 55 kDa antigen using MT55-1 MAb in mycobacterial culture filtrate before detecting it in clincal specimens. The minimal detectable concentration was 1.0 ㎍/m1 for M. tuberculosis culture filtrate and 100 ㎍/ml for sonic extracts of M. bovis BCG and M. marinum, respectively. But the 55 kDa antigen was not detected in sonic extracts of other mycobacterial species examined. Although further evaluations are required, this study suggests that the 55 kDa antigen may be of interest as potential diagnostic reagent.
김화중,송희열 건국대학교 1993 學術誌 Vol.37 No.2
Natural zeolites have recently been focused on due to their versatilities in possible industrial applications. Among several different types of domestic natural zeolites, clinoptilolite, in particular is one of highly prospective domestic natural zeolite. However, the isostructure of clinoptilolite, heulandite is always possible to coexist with clinoptilolite. Unfortunately, heulandite is thermally very unstable restricting its application to industrial process. In this research program, domestic natural zeolite, heulandite was chosen to study the effects of ion exchanges and heat-treatments on the thermal stability. Two different ion-exchanging experiments were carried out followed by heat-treatments at different temperatures. X-ray and IR spectroscopic analyses showed the enhancements in thermal stabilities of heulandite by Na+ cation exchanges.
콘크리트의 應力-變形關係에 關한 實驗的 硏究 : 鋼纖維 콘크리트에 關하여 About steel fiber concrete
金和中,權寧煥,李尙宰,全明熏 경북대학교 산업기술연구소 1990 産業技術硏究誌 Vol.18 No.-
The factors adopted in this experiment are volume fraction and the diameter and aspect ratio of fiber. The levels of factors are determined by existing studies. The effects of the diameter and mixing volume fraction and the aspect ratio for maximum strength, strain and tangent modulus are analyxed based on the linear multiple regression and we obtained regression equations as follows ; Maximum stress : σ_max=298.59820+95.78361φ-6.36043V_f+0.62067Ar Maximum strain at maximum stress : ε_max=0.021336+0.15746φ+0.05519V_f+0.00181Ar Tangent modulus : E=208209.80+87132.84φ-17286.69V_f-464.9181Ar where φ : diameter V_f : volume fraction Ar : Aspect Ratio