Induces Vasodilatation of Rat Mesenteric Artery in vitro Mainly by Inhibiting Receptor-Mediated Ca2+-Influx and Ca2+-Release

Yong-Xiao Cao,Jian-Pu Zheng,Jian-Yu He,Jie Li,Cang-Bao Xu,Lars Edvinsson 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.6

The purpose of this study was to investigate the effect of atropine on peripheral vasodilation and the mechanisms involved. The isometric tension of rat mesenteric artery rings was recorded in vitro on a myograph. The results showed that atropine, at concentrations greater than 1 µM, relaxed the noradrenalin (NA)-precontracted rat mesenteric artery in a concentration-dependent manner. Atropine-induced vasodilatation was mediated, in part, by an endothelium-dependent mechanism, to which endothelium-derived hyperpolarizing factor may contribute. Atropine was able to shift the NA-induced concentration-response curve to the right, in a non-parallel manner, suggesting the mechanism of atropine was not mediated via the α1-adrenoreceptor. The β- adrenoreceptor and ATP sensitive potassium channel, a voltage dependent calcium channel, were not involved in the vasodilatation. However, atropine inhibited the contraction derived from NA and CaCl2 in Ca2+-free medium, in a concentration dependent manner, indicating the vasodilatation was related to the inhibition of extracellular Ca2+ influx through the receptoroperated calcium channels and intracellular Ca2+ release from the Ca2+ store. Atropine had no effect on the caffeine-induced contraction in the artery segments, indicating the inhibition of intracellular Ca2+ release as a result of atropine most likely occurs via the IP3 pathway rather than the ryanodine receptors. Our results suggest that atropine-induced vasodilatation is mainly from artery smooth muscle cells due to inhibition of the receptor-mediated Ca2+-influx and Ca2+- release, and partly from the endothelium mediated by EDHF.

Development and mechanical properties of bagasse fiber reinforced composites

Cao, Yong,Goda, Koichi,Shibata, Shinichi The Korean Society for Composite Materials 2007 Advanced composite materials Vol.16 No.4

Environment-friendly composites reinforced with bagasse fiber (BF), a kind of natural fiber as the remains from squeezed sugarcane, were fabricated by injection molding and press molding. As appropriate matrices for injection molding and press molding, polypropylene (PP) and polycaprolactone-cornstarch (PCL-C) were selected, as a typical recyclable resin and biodegradable resin, respectively. The mechanical properties of BF/PP composites were investigated in view of fiber mass fraction and injection molding conditions. And the mechanical properties and the biodegradation of BF/PCL composites were also evaluated. In the case of injection molding, the flexural modulus increased with an increase in fiber mass fraction, and the mechanical properties decreased with an increase in cylinder temperature due to the thermal degradation of BF. The optimum conditions increasing the flexural properties and the impact strength were $90^{\circ}C$ mold temperature, 30 s injection interval, and in the range of 165 to $185^{\circ}C$ cylinder temperature. On the other hand, as to BF/PCL-C fully-green composites, both the flexural properties and the impact strength increased with an increase in fiber mass fraction. It is considered that the BF compressed during preparation could result in the enhancement in mechanical properties. The results of the biodegradability test showed the addition of BF caused the acceleration of weight loss, which increased further with increasing fiber content. This reveals that the addition and the quantities of BF could promote the biodegradation of fully-green composites.

Induces Vasodilatation of Rat Mesenteric Artery in vitro Mainly by Inhibiting Receptor-Mediated $Ca^{2+}$ -Influx and $Ca^{2+}$ -Release

Cao Yong-Xiao,Zheng Jian-Pu,He Jian-Yu,Li Jie,Xu Cang-Bao,Edvinsson Lars The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.6

The purpose of this study was to investigate the effect of atropine on peripheral vasodilation and the mechanisms involved. The isometric tension of rat mesenteric artery rings was recorded in vitro on a myograph. The results showed that atropine, at concentrations greater than 1$\mu$M, relaxed the noradrenalin (NA)-precontracted rat mesenteric artery in a concentration-dependent manner. Atropine-induced vasodilatation was mediated, in part, by an endothelium-dependent mechanism, to which endothelium-derived hyperpolarizing factor may contribute. Atropine was able to shift the NA-induced concentration-response curve to the right, in a non-parallel manner, suggesting the mechanism of atropine was not mediated via the ${\alpha}_1$-adrenoreceptor. The $\beta$-adrenoreceptor and ATP sensitive potassium channel, a voltage dependent calcium channel, were not involved in the vasodilatation. However, atropine inhibited the contraction derived from NA and $CaCl_2$ in $Ca^{2+}$-free medium, in a concentration dependent manner, indicating the vasodilatation was related to the inhibition of extracellular $Ca^{2+}$ influx through the receptor-operated calcium channels and intracellular $Ca^{2+}$ release from the $Ca^{2+}$ store. Atropine had no effect on the caffeine-induced contraction in the artery segments, indicating the inhibition of intracellular $Ca^{2+}$ release as a result of atropine most likely occurs via the IP3 pathway rather than the ryanodine receptors. Our results suggest that atropine-induced vasodilatation is mainly from artery smooth muscle cells due to inhibition of the receptor-mediated $Ca^{2+}$-influx and $Ca^{2+}$-release, and partly from the endothelium mediated by EDHF.

Simulating the performance of the reinforced concrete beam using artificial intelligence

Yong Cao,Ruizhe Qiu,Wei Qi Techno-Press 2023 Advances in concrete construction Vol.15 No.4

In the present study, we aim to utilize the numerical solution frequency results of functionally graded beam under thermal and dynamic loadings to train and test an artificial neural network. In this regard, shear deformable functionally-graded beam structure is considered for obtaining the natural frequency in different conditions of boundary and material grading indices. In this regard, both analytical and numerical solutions based on Navier's approach and differential quadrature method are presented to obtain effects of different parameters on the natural frequency of the structure. Further, the numerical results are utilized to train an artificial neural network (ANN) using AdaGrad optimization algorithm. Finally, the results of the ANN and other solution procedure are presented and comprehensive parametric study is presented to observe effects of geometrical, material and boundary conditions of the free oscillation frequency of the functionally graded beam structure.

miR-101 Inhibiting Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma through Downregulating Girdin

Cao, Ke,Li, Jingjing,Zhao, Yong,Wang, Qi,Zeng, Qinghai,He, Siqi,Yu, Li,Zhou, Jianda,Cao, Peiguo Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.2

miR-101 is considered to play an important role in hepatocellular carcinoma (HCC), but the underlying molecular mechanism remains to be elucidated. Here, we aimed to confirm whether Girdin is a target gene of miR-101 and determine the tumor suppressor of miR-101 through Girdin pathway. In our previous studies, we firstly found Girdin protein was overexpressed in HCC tissues, and it closely correlated to tumor size, T stage, TNM stage and Edmondson-Steiner stage of HCC patients. After specific small interfering RNA of Girdin was transfected into HepG2 and Huh7.5.1 cells, the proliferation and invasion ability of tumor cells were significantly inhibited. In this study, we further explored the detailed molecular mechanism of Girdin in HCC. Interestingly, we found that miR-101 significantly low-expressed in HCC tissues compared with that in matched normal tissues while Girdin had a relative higher expression, and miR-101 was inversely correlated with Girdin expression. In addition, after miR-101 transfection, the proliferation, migration and invasion abilities of HepG2 cells were weakened. Furthermore, we confirmed that Girdin is a direct target gene of miR-101. Finally we confirmed Talen-mediated Girdin knockout markedly suppressed cell proliferation, migration and invasion in HCC while downregulation of miR-101 significantly restored the inhibitory effect. Our findings suggested that miR-101/Girdin axis could be a potential application of HCC treatment.

Alkaloids from Lycoris caldwellii and their particular cytotoxicities against the astrocytoma and glioma cell lines

Peng Cao,Dong-Sheng Pan,Song Han,Chun-Yong Yu,Qing-Jie Zhao,Yan Song,Yong Liang 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.8

Phytochemical investigation of the ethanolextract of the bulbs of Lycoris caldwellii afforded fournew alkaloids, (?)-N-methoxylcarbonyl-nandigerine (1),(?)-N-methoxycarbonyl-lindcarpine (2), (?)-10-O-methylhernovineN-oxide (3), and (?)-3-hydroxy-anhydrolycorineN-oxide (4). Structural elucidation of all the compounds wereperformed by spectral methods such as 1D and 2D (1H-1HCOSY, HMQC, and HMBC) NMR spectroscopy, in additionto high resolution mass spectrometry. All the alkaloids werein vitro evaluated for their cytotoxic activities against eighttumor cell lines (BEN-MEN-1, CCF-STTG1, CHG-5, SHG-44, U251, BGC-823, HepG2, and SK-OV-3). Alkaloids 1and 2 exhibited particular cytotoxic activities against astrocytomaand glioma cell lines with IC50 of 9.2–11.3 lM and10.4–12.2 lM respectively.

miR-101 Inhibiting Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma through Downregulating Girdin

Peiguo Cao,Ke Cao,Jingjing Li,Yong Zhao,Qi Wang,Qinghai Zeng,Siqi He,Li Yu,Jianda Zhou 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.2

miR-101 is considered to play an important role in hepatocellular carcinoma (HCC), but the underlying molecular mechanism remains to be elucidated. Here, we aimed to confirm whether Girdin is a target gene of miR-101 and determine the tumor suppressor of miR-101 through Girdin pathway. In our previous studies, we firstly found Girdin protein was overexpressed in HCC tissues, and it closely correlated to tumor size, T stage, TNM stage and Edmondson-Steiner stage of HCC patients. After specific small interfering RNA of Girdin was transfected into HepG2 and Huh7.5.1 cells, the proliferation and invasion ability of tumor cells were significantly inhibited. In this study, we further explored the detailed molecular mechanism of Girdin in HCC. Interestingly, we found that miR-101 significantly low-expressed in HCC tissues compared with that in matched normal tissues while Girdin had a relative higher expression, and miR-101 was inversely correlated with Girdin expression. In addition, after miR-101 transfection, the proliferation, migration and invasion abilities of HepG2 cells were weakened. Furthermore, we confirmed that Girdin is a direct target gene of miR-101. Finally we confirmed Talen-mediated Girdin knockout markedly suppressed cell proliferation, migration and invasion in HCC while down-regulation of miR-101 significantly restored the inhibitory effect. Our findings suggested that miR-101/Girdin axis could be a potential application of HCC treatment.

Proteomic Approach Analysis of Mammary Membrane Proteins Expression Profiles in Holstein Cows

Yang, Yong-xin,Cao, Sui-zhong,Zhang, Yong,Zhao, Xing-xu Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.6

To investigate host defense mechanisms for protecting the mammary gland from mastitis infection, the membrane fraction of mammary tissues from Holstein cows was purified by differential velocity centrifugation, and then the sodium dodecyl sulfate-polyacrylamid gel electrophoresis (SDS-PAGE) separated proteins were identified by ion trap mass spectrometer equipped with a Surveyor high performance liquid chromatography (HPLC) system. A total of 183 proteins were identified. Bioinformatics software was applied to analyse physicochemical characteristics of the identified proteins and to predict biochemical function. These data may provide valuable information to investigate the mechanisms of mammary gland milk secretion and infectious disease, and enable a clear identification of proteins and potential protein targets for therapies.

Deformation Characteristics and Countermeasures of shallow and Large-span Tunnel Under-crossing the Existing Highway in Soft Soil: a Case Study

Cheng-yong Cao,Cheng-hua Shi,Ming-feng Lei,Li-min Peng,Ruxue Bai 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.8

Hongmian Road goes under Jihe highway by the way of tunnel in Shenzhen City, Guangdong Province, China. The tunnel hassome special characteristics such as shallow depth, large span, soft soil, busy traffic, and long distance under-crossing the operatinghighway, thus there is great difficulty and risk during its construction. A series of in-situ tests were conducted to reveal deformationcharacteristics during tunneling beneath the existing highway by the double-side-drift method (DSDM). The results show that initialconstruction method and measures can't meet the safety requirement of tunnel construction, and the level of risk is extremely high, sothe construction method need to be timely adjusted. By comprehensive analysis, the double-side-drift optimum method (DSDOM) isput forward, on the basis of which a series of optimum measures also have been put forward, such as reasonable constructionmethods, the suitable length of bench and excavation, soil reinforcement measures on tunnel face, optimizing construction process,closing primary support in time and speeding up the construction of secondary lining. Then the feasibility of this optimizedconstruction method and measures are verified by the numerical simulations. Finally, from feedbacks of monitoring results, theoptimum method and measures have been proved to be reasonable and feasible, and they can guarantee a successful pass beneath theoperating highway.

Rotational-motion compensation algorithm for the real-time generation of the holographic videos of 3-D objects

Cao, Hong Kun,Hwang, Yong Seok,Kim, Eun Soo 한국통신학회 2019 한국통신학회 학술대회논문집 Vol.2019 No.6