동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여

박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.

단클론성 감마글로부린병증에 의한 만성 탈수초성 신경병증

고진석,김승현,김주한,김명호,김덕언 대한신경과학회 1998 대한신경과학회지 Vol.16 No.5

금은화 추출물의 세포독성에 관한 연구

한종현,백승화,김일광,한두석 圓光大學校 韓醫學硏究所 1993 원광한의학 Vol.3 No.1

In order to investigate the cytotoxicity of Lonicerae Flos extract, their extracts were dissolved in distilled water and methanol. All material diluted from 10^-3to 10^-7(㎎/㎖) with distilled water putted in each well of 24 multidish cultured rat fibroblast for 48hours. The cell number was calculated at 2 days and cell shape takes photographed by inverted microscope at same day. The results were as follows: 1. The rate of cell multiplication was the lowest in water extract from 10^-3 to 10^-7(㎎/㎖) concentration and the highest in ether and hexane extract at 10^-7(㎎/㎖) concentration. 2. Index of cytotoxicity was the highest with 3 score in water extract at 10^-3(㎎/㎖) concentration and the lowest with 1 score in ether, ethyl acetate or hexane extract at same concentration. 3. The degeneration of cell shape and number was the severest in water extract of 10&-4(㎎/㎖) concentration, but ether and hexane extracts in same concentration were weaker than water extract. These results indicate that the cytotoxicity of extracts by ether and hexane from same Lonicerae Flos was weaker then water extract.

수막종의 Flow Cytometry 및 Nucleolar Organizer Regions(NORs)에 관한 비교연구

韓東錫,蔡暘錫,金仁仙,白承龍 고려대학교 의과대학 1990 고려대 의대 잡지 Vol.27 No.1

The flow cytometric DNA analysis and the Ag-NORs staining method have been reported to reflect the cellular activities and the proliferative rates of the normal and the neoplastic cells. Currently, these methods have attracted many interests from clinical fields in evaluating the patient regarding on the prognosis and the responsiveness to therapy of human tumors. The author analyzed thirty eight meningiomas(meningothelial 5, transitional 7, fibrous 5, psammomatous 2, atypical 11, hemangiopericytic 6 and hemangoblastic 2 cases) using flow cytometry and Ag-NORs staining in the paraffin embedded tissues. The results are summarized as follows : 1. In flow cytometric study, twenty eight cases were diploid and the remaining 10 cases were aneuploid. The latter included meningothelial 3, atypical 3, hemangiopericytic 2 and hemangioblastic 2 cases. 2. In Ag-NORs study, the mean mumber of Ag-NORs had a significant difference between classical types(1.46±O.21) and atypical meningiomas(1.88±0.36) including hemangiopericytic one(1.74±0.38) (P<0.05). 3. The cytometric S phase, G_(0)/G₁ phase and proliferation index and the mean number of Ag-NORs had a significant difference between classical diploid and atypical diploid meningiomas(P<0.005). 4. There was no significant relationship between cytometric S phase and the mean number of Ag-NORs in classical meningiomas.

차량관제시스템을 위한 Inverted DGPS 시스템

한승재,홍진석,지규인 건국대학교 1997 學術誌 Vol.41 No.2

Vehicle Monitoring System which can efficiently control and manage the resources like vehicles, cargos and services is required in many transportation related areas like ITS /IVHS. GPS or DGPS is widely used in determining the vehicle location in service. DGPS can glove more accurate position than GPS, but it needs an additional wireless communication link for DGPS correction reception from the DGPS reference station. But, in some applications lire tall or truck monitoring system, the user doesn't need to know the his accurate position. The DGPS correction can be performed in the monitoring station based on the informations from the GPS receiver in the user vehicle and then the monitor station can locate vehicle position accurately like conventional DGPS. For this case an additional communication link for DGPS RTCM is not necessary. This method is called inverted DGPS. In this paper we develop an IDGPS system algorithm and implement it using wireless data modem network and digital map. Test results show its effectiveness. Key Words : GPS, DGPS, Inverted DGPS, vehicle Monitoring System, RTCM

Mo 화학기상증착에 있어서 불순물 농도에 미치는 증착조건의 영향

배상석,서성교,홍은식,김승모,조미정,한혜정,이두성,김세훈,민석홍 국립7개대학공동논문집간행위원회 2001 공업기술연구 Vol.1 No.-

The thermal decomposition process of Mo(CO)_6 on chemical vapor deposition was investigated by analyzing the effects of deposition temperature, pressure, and reaction gas on the phase change of deposited films. Mo_2C was deposited at or below 350℃ due to the incompleteness of thermal decomposition of Mo(CO)_6, but Mo films was successfully deposited at 400℃ or higher temperatures. The variation of deposition pressure did not change the dependence of decomposition process on deposition temperature. The activation energy of surface reaction was 5.8 ㎉/mole.

캠퍼스 인트라넷 사용자 만족요인 연구

이형석,박승봉,한재민 한국인터넷비즈니스학회 2003 인터넷비지니스연구 Vol.4 No.2

본 연구는 최근 모든 대학들이 서비스를 제공하고 있는 캠퍼스 인트라넷에 대한 대학생들의 만족도를 분석하였다. 기존의 기업 정보시스템 평가에서 사용되어지고 있는 정보 요인, 시스템 요인 그리고 개인 요인들이 전반적인 사용자 만족에 어떠한 영향을 미치는지를 살펴보았다. 분석결과 정보 요인에서는 내용성과 접근성이 유의한 요인으로 개인 요인에서는 사용자 개인의 능력과 흥미가 유의하게 나타났다. 그리고 시스템 요인에서는 인트라넷 사용의 용이성과 사용자 인터페이스가 전반적인 사용자 만족에 영향을 미치는 요인으로 나타났다. 따라서 본 연구의 캠퍼스 인트라넷에 대한 만족 요인 분석결과를 토대로 효과적인 대학정보서비스 제공과 지속적인 수정 및 업데이트를 위한 정보를 제공할 수 있을 것으로 본다. This study analyzed that university students' satisfaction with campus Intranet provided by all of university in recent. In detail, this showed how information, system and individual factor being used in evaluation on corporate information system are affecting the overall user satisfaction. The result of the analysis shows that information content and information access in information factors, individual ability and interest in individual factors were significant factor. And easiness of Intranet use and user based interface in system factors affected significantly user satisfaction. Thus, this study provides the information for continuous correction and update as well as educational information service effectively.

알쯔하이머형 치매 환자에서 우울증의 유무에 따른 인지기능과 일상생활 수행능력의 차이

김승민,최태규,안석균,손석한,유계준,오병훈,오희철 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.2

연구목적 : 알쯔하이머형 치매 환자에게 있어서 중요한 행동증상중의 한가지인 우울증의 다양한 범위로 보고되고 있으며 우울증과 인지능력 및 기능적 능력과의 관계에 대한 연구가 매우 중요하게 부각되고 있다. 본 연구의 목적은 지역사회에 거주하는 경도 및 중등도의 알쯔하이머형 치매 환자를 대상으로 우울증이 일상생활 수행능력과 인지기능의 특정 영역과 연관이 있는지의 여부를 알아보기 위함이다. 방 법 : 본 연구대상은 경기도 광주군에 거주하는 65세 이상의 알쯔하이머형 치매 노인 48명이며, 한국판 Mini-Mental State Examination(이하 MMSE-K), 한국어판 신경행동학적 인지상태 검사(이하 한국어판 NCSE), 한국형 노인우울검사(이하 KGDS), 기본적인 일상생활 수행정도(이하 ADL), 그리고 도구적인 일상생활 수행정도(이하 IADL)를 연구도구로 하여 이들로부터 자료를 수집 조사하였다. 연구대상은 KGDS에 의해 우울 치매군과 비우울치매군으로 분류하였으며 두 군간의 상기 척도에 의해서 얻어진 결과를 χ²test 및 independent t-test를 이용하여 분석하였다. 결 과 : 우울 치매군과 비우울 치매군은 성별, 연령, 교육수준 및 MMSE-K 점수의 유의한 차이가 없었다. 우울 치매군은 비우울 치매군보다 ADL의 한 항목인 목욕하기에서 유의한 저하를 보였다. 우울 치매군은 비우울 치매군보다 IADL의 총점 및 식품점에 가서 장보기, 집안일(청소나 정리정돈), 금전 관리의 3개 항목에서 유의한 저하를 보였다. 그러나 우울 치매군은 비우울 치매군은 한국어판 NCSE를 이용한 인지기능의 영역별 비교에서는 유의한 차이를 보이지 않았다. 결 론 : 이상의 결과로 미루어 볼 때 알쯔하이머형 치매 환자에서의 우울증은 기본적인 일상생활 기능의 일부와 도구적인 일상생활 기능의 저하와 상당한 관련 있고, 이러한 우울증에 동반된 일상생활 기능의 저하는 인지기능의 영향에 기인하지 않는 것임을 시사된다. Objectives : Coexistence of depression is one of the important behavioral symptoms in patients with dementia of Alzheimer type. The study on the relationship between depression and cognitive abilities and /or functional abilities emerges as very important one. The aim of this study was to investigate whether depression is related to the impairment of daily activities and to certain areas of cognitive function in mild to moderate dementia of Alzheimer type. Methods : In this study, 48 patients with dementia of Alzheimer type among elderly community-residents in Kwangju, Kyunggi province, age 65 and older, were finally included. We examined their demographics and administered Korean Version of the Mini-Mental State Examination(MMSE-K). Korean Version of the Neurobehavioral Cognitive Status Examination (Korean version of NCSE), Korean Form of Geriatric Depression Scale(KGDS), Activities of Daily Living(ADL), and Instrumental Activities of Daily Living(IADL) to the subjects. We classified the subjects into two groups, dementia with and without depression on the basis of KGDS scores. The two groups were compared using χ²test and independent t-test. Results : The results show that the two groups did not differ in sex, age, education, and scores of MMSE-K. The depressed dementia group showed significantly poorer performance in bathing, one item of ADL, than the non-depressed dementia group. The depressed dementia group showed significantly more impairment in total scores of IADL and each score of three item(shopping, housework, handling finances) than the non-depressed dementia group. However, the two groups showed no significant difference in five major cognitive ability areas of Korean version of NCSE. Conclusion : These results suggest that depression in patients with dementia of Alzheimer type is highly related to the functional impairments, instrumental activities of daily living and part of activities of daily living, beyond the effects of cognitive impairments.

비선형 웹 이송 시스템의 장력제어

윤석찬,백승훈,정진태,한창수 韓國工作機械學會 2000 한국생산제조학회지 Vol.9 No.5

This paper presents the study of the tension control in a web transfer system. In this study the sliding mode controller is applied to a time-varying nonlinear mathematical model. The model was derived to consider the effects of changing the roll rad;us in tension variation during winding and unwinding. The uncertainty in modeling may be due to unmodelled dynamics, on variations in system model. Designed sliding mode controller made the system error always staying in the suggested surface from the beginning. Through this, system is maintained to be robust against a disturbance and uncertainty. To verify the designed controller has a good performance, various inputs such as desired velocity, step input, and trapezoidal input are applied. When the sliding mode controller was used, the system(the tension control) performance was improved comparing to the PID controller. The robustness of the controller with respect to an estimation error was verified through simulations.

저선량 감마선 조사가 왕겨의 항산화능에 미치는 영향

정석문,김정한,김동열,육홍선,변명우,이승철 慶南大學校 附設 基礎科學硏究所 2002 硏究論文集 Vol.16 No.-

왕겨에 200, 400, 600, 800 및 1000 Gy의 저선량의 감마선을 조사하여 항산화능의 변화를 관찰하였다. 총 페놀 화합물의 양은 무처리구의 경우 0.184 mM를 나타내었고, 200 Gy로 감마선을 조사하였을 때는 0.215 mM로 약간 증가함을 보였으나 이후 차츰 감소하였다. 전자 공여능은 무처리구에 비해 처리구의 경우 조사 선량별로 조금씩의 차이만 있을 뿐, 조사 선량에 대한 왕겨의 전자 공여능은 유의적인 큰 변화 없이 비슷한 활성을 보였다. Fe^2+-ions 킬레이팅 활성은 무처리구의 경우 68.25%를 나타내었으며, 200, 400, 600 및 800 Cy 에서는 각각 86.45, 85.61, 86.65 및 82.84%로서 무처리구보다는 증가함을 보였으나, 1000 Gy에서는 74.67%로 감소하였다. 지질 과산화 억제능은 무처리구에 비해 처리구는 약간의 증가를 보였으나 효과는 미비하였다. We studied the effect of small-dose of γ-irradiation to rice hull(RH) for its antioxidant ability. The RH was treated with 200, 400, 600, 800, and 1000 Gy of γ-ray at 70 Gy/min. Total phenol amount of unirradiated RH was 0,184 mM, after irradiation at 200 Gy it increased to 0,215 mM, while irradiation above 400 Gy up to 1000 Gy decreased that to unirradiated level. The electron donating ability of RH did not show significant change depend on irradiation. The chelating ability for Fe^2+ of unirradiated RH was 68.25 %, while that of 200, 400, 600 and 800 Gy irradiated RH showed 86.45, 85.61, 86.65 and 82.84 % respectively, but, 1000 Gy was decreased to 74.67 %. According to TBARS analyses, inhibition ability of lipid peroxidation by RH was not affected by γ-irradiation.