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RISS 인기검색어
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- 저자 : ( Junli Zhu ) (검색결과 460 건)
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Junli Zhu,Xuzheng Huang,Fang Zhang,Lifang Feng,Jianrong Li 한국미생물학회 2015 The journal of microbiology Vol.53 No.12
We investigated the quorum sensing (QS) system of Shewanella baltica and the anti-QS related activities of green tea polyphenols (TP) against spoilage bacteria in refrigerated large yellow croaker. Autoinducer-2 (AI-2) and the diketopiperazines (DKPs) cyclo-(L-Pro-L-Leu) and cyclo-(L-Pro-L-Phe) were detected in the culture extract of S. baltica XH2, however, no N-acylhomoserine lactones (AHLs) activity was observed. Green TP at sub-inhibitory concentrations interfered with AI-2 and DKPs activities of S. baltica without inhibiting cell growth and promoted degradation of AI-2. The green TP treatment inhibited biofilm development, exopolysaccharide production and swimming motility of S. baltica in a concentration- dependent manner. In addition, green TP decreased extracellular protease activities and trimethylamine production in S. baltica. A transcriptional analysis showed that green TP repressed the luxS and torA genes in S. baltica, which agreed with the observed reductions in QS activity and the spoilage phenotype. Epigallocatechin gallate (EGCG)-enriched in green TP significantly inhibited AI-2 activity of S.baltica. These findings strongly suggest that green TP could be developed as a new QS inhibitor for seafood preservation to enhance shelf life.
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Zhu, Xi,Xu, Yingjie,Solis, Luisa M.,Tao, Wei,Wang, Liangzhe,Behrens, Carmen,Xu, Xiaoyang,Zhao, Lili,Liu, Danny,Wu, Jun,Zhang, Ning,Wistuba, Ignacio I.,Farokhzad, Omid C.,Zetter, Bruce R.,Shi, Jinjun National Academy of Sciences 2015 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.112 No.25
<P><B>Significance</B></P><P>This study developed a new generation lipid–polymer hybrid nanoparticle platform for effective systemic delivery of small interfering RNA (siRNA) to tumors, which represents a challenging hurdle for the widespread application of RNA interference (RNAi) in cancer research and therapy. With promising in vivo features such as long blood circulation, high tumor accumulation, and effective gene silencing, the hybrid siRNA nanoparticles were successfully used to reveal and validate a putative therapeutic target, Prohibitin1 (PHB1), in non-small cell lung cancer treatment. In vivo antitumor efficacy results and human tissue microarray analysis further suggested the feasibility of utilizing PHB1 siRNA nanoparticles as a novel therapeutic agent. This hybrid RNAi nanoparticle platform may serve as a valuable tool for validating potential cancer targets and developing new cancer therapies.</P><P>RNA interference (RNAi) represents a promising strategy for identification and validation of putative therapeutic targets and for treatment of a myriad of important human diseases including cancer. However, the effective systemic in vivo delivery of small interfering RNA (siRNA) to tumors remains a formidable challenge. Using a robust self-assembly strategy, we develop a unique nanoparticle (NP) platform composed of a solid polymer/cationic lipid hybrid core and a lipid-poly(ethylene glycol) (lipid-PEG) shell for systemic siRNA delivery. The new generation lipid–polymer hybrid NPs are small and uniform, and can efficiently encapsulate siRNA and control its sustained release. They exhibit long blood circulation (<I>t</I><SUB>1/2</SUB> ∼8 h), high tumor accumulation, effective gene silencing, and negligible in vivo side effects. With this RNAi NP, we delineate and validate the therapeutic role of Prohibitin1 (PHB1), a target protein that has not been systemically evaluated in vivo due to the lack of specific and effective inhibitors, in treating non-small cell lung cancer (NSCLC) as evidenced by the drastic inhibition of tumor growth upon PHB1 silencing. Human tissue microarray analysis also reveals that high PHB1 tumor expression is associated with poorer overall survival in patients with NSCLC, further suggesting PHB1 as a therapeutic target. We expect this long-circulating RNAi NP platform to be of high interest for validating potential cancer targets in vivo and for the development of new cancer therapies.</P>
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Aberrant Expression of CCAT1 Regulated by c-Myc Predicts the Prognosis of Hepatocellular Carcinoma
Zhu, Hua-Qiang,Zhou, Xu,Chang, Hong,Li, Hong-Guang,Liu, Fang-Feng,Ma, Chao-Qun,Lu, Jun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.13
Background: CCAT1 has been reported to be linked with pathogenesis of malignancies including colon cancer and gastric cancer. However, the regulatory effect of CCAT1 in hepatocellular carcinoma (HCC) remains unclear. The purpose of this research was to identify any role of CCAT1 in the progression of HCC. Materials and Methods: Real time-PCR was performed to test the relative expression of CCAT1 in HCC tissues. A computation screen of CCAT1 promoter was conducted to search for transcription-factor-binding sites. The association of c-Myc with CCAT1 promoter in vivo was tested by Pearson correlation analysis and chromatin immunoprecipitation assay. Additionally, Kaplan-Meier analysis and Cox proportional hazards analyses were performed. Results: c-Myc directly binds to the E-box element in the promoter region of CCAT, and when ectopically expressed increases promoter activity and expression of CCAT1. Moreover, Kaplan-Meier analysis demonstrated that the patients with low expression of CCAT1 demonstrated better overall and relapse-free survival compared with the high expression group. Cox proportional hazards analyses showed that CCAT1 expression was an independent prognostic factor for HCC patients. Conclusions: The findings demonstrated CCAT1, acting as a potential biomarker in predicting the prognosis of HCC, is regulated by c-Myc.
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Zhu Jun,Li Shaolong,Song Dandan,Han Qiaoli,Li guanghua 대한전기학회 2018 Journal of Electrical Engineering & Technology Vol.13 No.4
For the problem that the complexity of 3-D modeling and multi parameter optimization, as well as the uncertainty of the winding factor of axial flux permanent magnet generator with coreless windings. The complex 3-D model was simplified into 2-D analytic model, and an analytical formula for the winding factor that adapting different coreless stator winding is proposed in this paper. The analytical solution for air-gap magnetic fields, no-load back EMF, electromagnetic torque, and efficiency are calculated by using this method. The multiple objective and multivariable optimization of the maximum fundamental and the minimum harmonic content of back EMF are performed by using response surface methodology. The proposed optimum design method was applied to make a generator. The generator was tested and the calculated results are compared with the proposed method, which show good agreements.
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Zhu, Gen-Hai,Wang, Sheng-Tan,Yang, Zhao-Xin,Cai, Jun-Hong,Chen, Chun-Ying,Yao, Mao-Zhong,Hong, Lan,He, Guo-Li,Yang, Shu-Ying Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9
Objective: Nude mice with orthotopic transplantation of human ovarian epithelial cancer were used to investigate screening criteria for paraneoplastic normal ovarian tissue and the security of the freezing and thawing for ovarian tissue transplantation. Methods: Expression of CK-7, CA125, P53, survivin, MMP-2/TIMP-2 in paraneoplastic normal ovarian tissues were detected by RT-PCR as well as immunohistochemistry. The tissues of the groups with all negative indicators of RT-PCR, all negative indicators of immunohistochemistry, negative expression of CK-7, CA125 and survivin, positive expression of CK-7, CA125 and survivin, cancer tissues and normal ovarian tissues of nude mice were used for freezing and thawing transplantation, to analyze overt and occult carcinogenesis rates after transplantation. Results: When all indicators or the main indicators, CK-7, CA125 and survivin, were negative, tumorigenesis did not occur after transplantation. In addition the occult carcinogenesis rate was lower than in the group with positive expression of CK-7, CA125 and survivin (P<0.01). After subcutaneous and orthotopic transplantation of ovarian tissues, rates did not change (P>0.05). There was no statistical significance among rates after transplantation of ovarian tissues which were obtained under different severity conditions (P>0.05). Conclusion: Negative expression of CK-7, CA125 and survivin can be treated as screening criteria for security of ovarian tissues for transplantation. Immunohistochemical methods can be used as the primary detection approach. Both subcutaneous and orthotopic transplantation are safe. The initial severity does not affect the carcinogenesis rate after tissue transplantation. Freezing and thawing ovarian tissue transplantation in nude mice with human epithelial ovarian carcinoma is feasible and safe.
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Zhu Jun,Li Guanghua,Cao Di,Zhang Zhenyi,Li Shuaihui 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.2
A 90° Halbach permanent magnet array coreless axial fux permanent magnet synchronous generator for wind power generation is compared with a conventional axial fux permanent magnet generator with cut cake type permanent magnet. 2D analytic model of generator is established. The air gap magnetic felds of two generators are analyzed by analytical method, and the air gap magnetic density and harmonic content are analyzed. Then the infuence of diferent parameters on voltage total harmonic distortion is analyzed by changing the pole arc coefcient, air gap length, permanent magnet thickness and number of turns. Two prototypes of generators are manufactured, and the correctness of the simulation results is verifed by experiments.
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Jun Zhu,Yao Li,Ying-Ying Tang,Jian-Qi Liu,Yi-Yu Chen,Zhongxian LV,Fang Liu 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1
The Hemipteran predator, Cyrtorhinus lividipennis, feeds on the eggs and nymphs of rice planthoppers and leafhoppers. We previously demonstrated that sublethal concentrations of imidacloprid stimulated the reproduction of C. lividipennis. Considering the essential roles of juvenile hormone (JH) in insect reproduction, we speculated that sublethal concentrations of imidacloprid may stimulate the reproduction of C. lividipennis by regulating JH level. To test this, we cloned C. lividipennis JH acid methyl transferase (ClJHAMT) and JH esterase (ClJHE), which are responsible for JH biosynthesis and degradation genes, respectively. We then knocked down ClJHAMT by injecting dsRNA into C. lividipennis nymphs and found that emerging female adults exhibited 88.8% lower expression of the vitellogenin gene (ClVg) and the number of eggs was reduced by 41.5% as compared with controls. Silencing ClJHE increased ClVg mRNA expression by 275.0% but did not affect fecundity. A sublethal concentration of imidacloprid (LC 20 ) increased the JH titer in females by 35.3% and 60.6% at 24 and 48 h post-emergence, respectively. In treatments containing both imidacloprid and dsJHAMT, the silencing of CLJHAMT reduced the number of eggs produced by adult females by 21.4% as compared to the control (imidacloprid + dsGFP). Our results indicated that sublethal concentration of imidacloprid may induce C. lividipennis reproduction by upregulating JH level via JHAMT. The finding could provide valuable information for improved integration of C. lividipennis and insecticides in pest management.
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Prognostic value of programmed death-ligand 1 (PD-L1) expression in ovarian clear cell carcinoma
Jun Zhu,Hao Wen,Rui Bi,Yong Wu,Xiaohua Wu 대한부인종양학회 2017 Journal of Gynecologic Oncology Vol.28 No.6
Objective: Programmed death-ligand 1 (PD-L1) was expressed in various tumors andantibodies targeting its receptor programmed cell death-1 (PD-1) are emerging cancertherapeutics. This study was designed to evaluate the expression of PD-L1 and its correlationwith clinicopathologic features and clinical outcomes in ovarian clear cell carcinoma (OCCC). Methods: The PD-L1 expression was measured by tissue-microarray-basedimmunohistochemistry from 122 eligible patients diagnosed with OCCC. The associations ofclinicopathologic features with progression-free survival (PFS) and overall survival (OS) wereanalyzed by Kaplan-Meier method and multivariate analysis was further performed by Coxregression model. Results: Overall, high PD-L1 expression (PD-L1high) was observed in 44.7% (55/123) ofOCCC patients, and was strongly associated with advanced stages (p=0.020), positive asciticfluid (p=0.016), platinum-resistant (PR) disease (p=0.045), and recurrence (p=0.038). Moreover, patients with PD-L1high were associated with poorer OS (hazard ratio [HR]=2.877;p=0.001) and PFS (HR=1.843; p=0.021) than those with low PD-L1 expression (PD-L1low). In subgroup analysis, PD-L1high patients experienced a poorer PFS (HR=1.926; p=0.044)and OS (HR=2.492; p=0.021) than PD-L1low cases among advanced stages (III–IV), but thisdifference was not observed in stage I–II patients. Meanwhile, PD-L1high was associated withpoorer prognosis than PD-L1low in PR patients (OS, HR=2.253; p=0.037; PFS, HR=1.448;p=0.233). Multivariate analysis revealed that PD-L1high and advanced stages (III–IV) wereadverse independent prognosticators for both PFS (HRPD-L1=2.0; pPD-L1=0.038; HRstage=10.2;pstage<0.001) and OS (HRPD-L1=3.0; pPD-L1=0.011; HRstage=14.3; pstage<0.001). Conclusion: PD-L1high might serve as a risk factor for PFS and OS in patients with OCCC. It ispossible that immunotherapy targeting PD-L1 pathway could be used in OCCC.
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