溫室保溫커튼의 材料特性에 따른 保溫效果分析

金榮福,金容換,李昇揆,金成泰,羅又禎,閔永鳳,朴重春,崔東烈 慶尙大學校 1991 論文集 Vol.30 No.2

The effects of the material properties of thermal curtains upon reduction of the greenhouse heat loss were studied. A theoretical analysis procedure with equations and computer programs was developed to estimate them by using energy balance principles. Curtain effectiveness for every three kinds pf value level of the material properties were studied and compared. The results of this study would be useful for the developent and management of greenhouse thermal curtains.

엘라스타제의 토끼에 대한 최기형시험

안병옥,백남기,이순복,김원배,양중익,이영순,임윤규 A.R. Liss 1990 ENVIRONMENTAL MUTAGENS AND CARCINOGENS Vol.10 No.2

Pocine Adenovirus-3의 E1B Region의 鹽基序列 分析

朴鍾賢,宋載永,李重馥,玄芳勳,安東濬,車相昊,裵用泰,姜永源,Reddy, P S,全茂炯,安壽煥 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

돼지 아데노바이러스(PAV-3). 6618주의 EIB region이 包含되어 있는 map unit 4.0에서 9.7까지의 유전자에 대한 1,984 bp의 염기서열을 決定하였으며, 이 結果를 알려진 여러 아데노바이러스 유전자와 비교하여 다음과 같은 결과를 얻었다. 1. PAV-3의 EIB유전자는 10개의 ORF로 구성되어 있으며, 그 중 아데노바이러스의 단백질과 유사성이 있는 것은 ORF1, ORF2 및 ORF3이었다. ORF1은 Ad41의 19kd 와 BAV-2에서의 EIB ORF2에서의 아미노산의 一致率은 각각 32%와 31%이었다. 2. ORF2는 Ad2 55kd protein과 tupaia adenovirus 44kd protein가 각각 34%로 아미노산 一致率이 가장 높았으며, Ad41의 52kd protein. BAV-3의 EIB ORF3에서도 33%의 一致率을 보였다. 3. ORF1은 61-666 uncleotide (606 bp), ORF 2에서는 429-1,850 uncleotide (1,422 bp)의 부위로 각각 202, 474 a.a로 構成되었으며, 예상되는 분자량은 20 kd와 52 kd이었다. 4. ORF3는 hexon-associated pIX유전자로 추정되며 내부에 1개의 polyadenylation signal(ATAAA)이 1938-1942 uncleotide에 위치하였으며, 이 부위는 TATA box (1937-1942 uncleotide)와 중복되어 존재하였다. Porcine adenovirus type 3 (PAV-3) does not cause severe infection in pigs. Adenovirus has been suggestive of live vaccine vector carrying foreign gene. One of insertion regions is delayed early (EIB) region. However, EIB region of PAV-3 has not been molecularly characterized to date. Nucleotide sequence of EIB of PAV-3 was determined. The EIB region was composed of 1,984 bp and located between 4.0 and 9.7 map units. Three potential open reading frames(ORFs) with low level of homology to other adenoviruses and a polyadenylation signal were identified in the rightward direction of genome. The nucleotide and the predicted amino acid sequences of EIB were compared to those of human and animal adenoviruses. One of the three potential ORFs. ORF1 encoded a polypeptide homologous to bovine adenovirus type 2(BAV-2) ORF2 and human adenovirus type 41(Ad41) 19 kd protein. ORF2 encoded a polypeptide homologous to human adenovirus type 2(Ad2) 55 kd protein, bovine adenovirus type 3(BAV-3) ORF3 and porcine adenovirus type 4(PAV-4) ORF2. The predicted protein of ORF1 had homology to those of Ad41 and BAV-2 with 32 and 31% respectively, whereas the deduced protein of ORF2 had homology to those of Ad2. BAV-3 and PAV-4 with 34, 33 and 29%, respectively.

돼지 췌장 유래 엘라스타제의 항원성 시험

김순회,백남기,안병옥,이상득,이순복,김원배,양중익 A.R. Liss 1990 ENVIRONMENTAL MUTAGENS AND CARCINOGENS Vol.10 No.2

효과적인 하중운반체계의 동역학적 연구

서국웅,양점홍,윤양진,황영성,이중숙,김용재,유경복 釜山大學校 附設 體育科學硏究所 1994 體育科學硏究所 論文集 Vol.10 No.-

The purpose of this study was to get the quantitative data of effect of two different load carrying system on Kinematics and Kinetics. The subjects for the research were 2-adults. The mechanism of each load carrying pattern was measured by a motion analylizer, a forceplatform and EMG system. The conclusions were get from Kinematics variables and Kinetics variables. 1. It was not effected at the vertical reaction force(Fz) of left, right foot according to the big value the 1st peack of 10kg, 15kg fore, back-pack carrying system, 20 kgw, 30 kgw back-pack carrying system in walking. But it was effected at the vertical reaction force(Fz) on 30 kgw back-pack carrying system in walking. 2. It was not effected at the Anterior-Posterior reaction force(Fx) of left, right foot according to high value of the 1st peack on 10 kgw, 15 kgw fore, back-pack carrying in walking. But, it was effected on the 20 kgw, 30 kgw only back-pack carrying in walking. 3. It was effected at the left-right reaction force(Fy) of left-right foot according to the big value of the 2nd peak on 10 kgw, 15 kgw fore-backpack carrying system in walking. It was more effective than 20 kgw, 30 kgw back-pack carrying system in walking. There was not disorder force(Fx) on 10 kgw, 15 kgw fore-backpack carrying system in walking. 4. Analysis of computer graphic, on the pack carrying system in walking. It was efficient in 10 kgw, 15 kgw fore-backpack carrying system in walking. But, it was efficient on 20 kgw, 30 kgw backpack carrying in walking. Castrocnemius, Tibialis anterior, Vastus lateralis, Vastus medialis, are stressed to bend their body foreward. So it occurred fatigue fast, injured muscles and joints. 5. Extension anyles and flection angles of the knee joint and the hip joint on 10 kgw, 15 kgw fore-backpack carrying system in walking showed less than those on 20 kgw, 30 kgw backpack carrying system in walking. It was efficient on the 10 kgw, 15 kgw fore-backpack carrying system in walking. 6. Magnitudes of EMG of Castrocnemius, Tibialis anterior, Vastus lateralis, Vastus medialis, were increased according to increasing weight road and were decreased on the 20 kgw, 30 kgw back-pack carrying system in walking.

Genetic diversity of the Korean field strains of porcine reproductive and respiratory syndrome virus

Lee, Jung-Ah,Lee, Nak-Hyung,Lee, Joong-Bok,Park, Seung-Yong,Song, Chang-Seon,Choi, In-Soo,Lee, Sang-Won Elsevier 2016 INFECTION GENETICS AND EVOLUTION Vol.40 No.-

<P><B>Abstract</B></P> <P>Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically significant diseases in the swine industry. The PRRS virus (PRRSV) has genetically diverse populations, like other RNA viruses, and various field strains continue to be reported worldwide. The molecular epidemiological study of PRRSV can provide important data for use in controlling the disease. In this study, 50 oral fluid samples from conventional farms in Korea were taken to analyze nucleotide sequences of the open reading frame 5 of PRRSV. The viruses present in more than 80% of oral fluid samples genetically originated from the type 2 PRRSV, which is North American (NA) lineage. In addition 8.9% of samples contained both of the type 1 PRRSV, which is European (EU) lineage and the type 2 PRRSV. About 60% of farms involved in this study had more than two strains of PRRSV. In phylogenetic analysis, the Korean field strains of PRRSV detected from the oral fluid samples were divided into several subgroups: four subgroups of Korean field strains clustered with the type 1 PRRSV, and other five subgroups of Korean field strains clustered with the type 2. These results suggest that the type 2 PRRSV is more prevalent than the type 1 in Korea and heterologous strains of PRRSV can simultaneously infect a single pig farm.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The North American genotype of PRRSV is prevalently isolated in Korea than European genotype. </LI> <LI> Heterologous strains or genotypes of PRRSV simultaneously existed in a farm. </LI> <LI> Lelystad-like strain and P129-like strains were first detected in Korea and belonged to new subgroup by phylogenetic analysis. </LI> </UL> </P>

DNA barcoding techniques for avian influenza virus surveillance in migratory bird habitats.

Lee, Dong-Hun,Lee, Hyun-Jeong,Lee, Youn-Jeong,Kang, Hyun-Mi,Jeong, Ok-Mi,Kim, Min-Chul,Kwon, Ji-Sun,Kwon, Jun-Hun,Kim, Chang-Bae,Lee, Joong-Bok,Park, Seung-Yong,Choi, In-Soo,Song, Chang-Seon [Wildlife Disease Association] 2010 JOURNAL OF WILDLIFE DISEASES Vol.46 No.2

<P>Avian influenza virus (AIV) circulates among free-ranging, wild birds. We optimized and validated a DNA barcoding technique for AIV isolation and host-species identification using fecal samples from wild birds. DNA barcoding was optimized using tissue and fecal samples from known bird species, and the method was shown to distinguish 26 bird species. Subsequently, fecal samples (n=743) collected from wild waterfowl habitats confirmed the findings from the laboratory tests. All identified AIV-positive hosts (n=35) were members of the order Anseriformes. We successfully applied the DNA barcoding technique to AIV surveillance and examined AIV epidemiology and host ecology in these wild waterfowl populations. This methodology may be useful in the design of AIV surveillance strategies.</P>

Application of DNA barcoding technique in avian influenza virus surveillance of wild bird habitats in Korea and Mongolia.

Lee, Dong-Hun,Lee, Hyun-Jeong,Lee, Yu-Na,Lee, Youn-Jeong,Jeong, Ok-Mi,Kang, Hyun-Mi,Kim, Min-Chul,Kwon, Ji-Sun,Kwon, Jun-Hun,Lee, Joong-Bok,Park, Seung-Yong,Choi, In-Soo,Song, Chang-Seon American Association of Avian Pathologists [etc.] 2010 Avian diseases Vol.54 No.1

<P>In a previous study, we optimized DNA barcoding techniques for avian influenza virus (AIV) isolation and host identification, using fecal samples from wild birds, for high-throughput surveillance of migratory waterfowls. In the present study, we surveyed AIV in Mongolia during the breeding season and, subsequently, in Korea in winter, to compare prevalent AIV subtypes and hosts using DNA barcoding. In Korea, H4 and H5 subtypes were the most abundantly detected HA subtypes, and most AIVs were isolated from the major population (mallards, Anas platyrhynchos) of wild bird habitats. On the other hand, in Mongolia, H3 and H4 subtypes were the most abundantly detected HA subtypes, and most AIVs were isolated from a small population of wild bird habitats that were not visible at the sampling site. In conclusion, AIV isolation using fecal samples, accompanied with DNA barcoding techniques as a host bird species identification tool, could be useful for monitoring major and minor populations of wild bird habitats. Further, continuous, and large-scale surveillance could be helpful for understanding the AIV epidemiology, evolution, and ecology in wild waterfowl.</P>

Isolation and characterization of a novel H9N2 influenza virus in Korean native chicken farm.

Lee, Yu-Na,Lee, Dong-Hun,Park, Jae-Keun,Lim, Tae-Hyun,Youn, Ha-Na,Yuk, Seong-Su,Lee, Youn-Jeong,Mo, In-phil,Sung, Haan-Woo,Lee, Joong-Bok,Park, Seung-Yong,Choi, In-Soo,Song, Chang-Seon American Association of Avian Pathologists [etc.] 2011 Avian diseases Vol.55 No.4

<P>An outbreak of avian influenza, caused by an H9N2 low-pathogenic avian influenza virus (AIV), occurred in a chicken farm and caused severe economic losses due to mortality and diarrhea. AIV was isolated and identified in a sample from an affected native Korean chicken. Genetic analysis of the isolate revealed a high sequence similarity to genes of novel reassortant H9N2 viruses isolated from slaughterhouses and live bird markets in Korea in 2008 and 2009. Animal challenge studies demonstrated that the replication kinetics and pathogenicity of the isolate were considerably altered due to adaptation in chickens. Vaccine protection studies indicated that commercial vaccine was not able to prevent virus shedding and clinical disease when chickens were challenged with the isolate. These results suggest that the novel H9N2 virus possesses the capacity to replicate efficiently in the respiratory system against vaccination and to cause severe disease in domestic chickens. The results also highlight the importance of appropriate updating of vaccine strains, based on continuous surveillance data, to prevent the possibility of a new H9N2 epidemic in Korea.</P>

A Retrospective Study on the Effects of Expanded Program of Test and Slaughter for Eradication and Management of Bovine Tuberculosis among Dairy Cattle in Korea

Lee,Won-Chang,Lee,Sang-Mog,Lee,Tae-Jong,Lee,Joong-Bok 건국대학교 동물자원연구센터 1999 動物資源硏究誌 Vol.20 No.-

國內에서 牛型結核 發生의 豫防管理를 위하여 實施되고 있는 튜버클린 檢索과 陽性反應된 젖소를 殺處分하는 方法에 대하여 그 成果를 推移(retrospective study) 하고자 試圖하고, 1965年부터 1997年度 사이의 農林部刊行 統計資料를 收集 醫學統計學的 分析을 한 結果, 젖소의 튜버클린 陽性反應에 의한 牛型結核의 罹患率 每10萬頭當 最低 14.9頭부터 最高 669.3頭에 이르고 있었다. 1973年度부터 HCSM과 PPD 튜버클린 檢索을 竝行한 결과 그 效果가 있어 1990年度까지는 陽性反應率이 순조럽게 減少?형勢를 보였으나, 1997年度부터는 다시 多少 增加되는 傾向을 보이고 있었다. 以上의 結果로 미루어 보아 現在 進行되고 있는 젖소에 대한 튜버클린 檢索과 陽性反應된 젖소의 殺處分法은 酪農先進國의 境遇에서와 같이 繼續進行되어야 할 것으로 본다. This study was aimed at making a retrospective observation of the morbidity rate of bovine tuberculosis with tuberculin reactors through the effects on the expanded program of test and slaughter for eradication and management among dairy cattle in Korea, from 1965 to 1997. The morbidity rate of tuberculin reactors during the period varied from 14.9 to 669.3 per 100,000 dairy population, however, those tended to decreased from 1975 to 1990, but the rate of reactor were trended to increased after 1991. In conclusion, expanded program of test and slaughter reduced the incidence cases and morbidity rates of bovine tuberculosis with tuberculin reactor available for management of dairy cattle in Korea, when the tuberculin of HCSM and PPD together used after 1973. Finally, it is our hope that these information could be use for reference in further study of the field of veterinary public health and preventive medicine.