http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
HPLC-UV 방법을 이용한 향부자 분석법 개발과 국산 및 중국산 향부자의 패턴비교
서지윤(Jiyun Seo),김진아(Jinah Kim),김성건(Sungun Kim),유정(Ting Liu),황완균(Wan Kyunn Whang) 대한약학회 2012 약학회지 Vol.56 No.5
A high-performance liquid chromatography (HPLC) combined with ultraviolet (UV) method for the simultaneous determination of α-cyperone and nootkatone was developed for the quality control of Cyperus rotundus Linne. The separation was performed on a KR100-5C18 (4.6×250 mm) column, and an elution gradient composed of methanol and water with a flow-rate of 1.0 ml/min. Detection wavelength was set at 254 nm. The optimum extraction for the detection of the α-cyperone and nookatone was achieved by ultrasonic with methanol for an hour. Two marker compounds α-cyperone and nootkatone in Cyperi Rhizoma showed good linearity (R2>0.999) in the concentration range of 12.5 μg/ml to 200 μg/ml. The developed method provided satisfactory precision and accuracy with overall intra-day and inter-day variations of 0.04~1.23% and 0.08~0.68%, respectively, and the overall recoveries of 97.45~105.58% for the two compounds analyzed. Additionally, a difference was observed in the cluster analysis and principal component analysis between Cyperi Rhizoma in Korea and China. The result demonstrated that the principal component analysis is useful to distinguish between Cyperi Rhizoma in Korea and China.
Jiyun Lee,Chang Youn Lee,박준희,Hyang-Hee Seo,신선혜,Byeong-Wook Song,김일권,김상우,이세형,박종철,Soyeon Lim,황기철 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-
Osteoarthritis (OA) is a common joint disease that results from the disintegration of joint cartilage and the underlying bone. Because cartilage and chondrocytes lack the ability to self-regenerate, efforts have been made to utilize stem cells to treat OA. Although various methods have been used to differentiate stem cells into functional chondrocytes, the currently available methods cannot induce stem cells to undergo differentiation into chondrocyte-like cells without inducing characteristics of hypertrophic chondrocytes, which finally lead to cartilage disintegration and calcification. Therefore, an optimized method to differentiate stem cells into chondrocytes that do not display undesired phenotypes is needed. This study focused on differentiating adipose-derived stem cells (ASCs) into functional chondrocytes using a small molecule that regulated the expression of Sox9 as a key factor in cartilage development and then explored its ability to treat OA. We selected ellipticine (ELPC), which induces chondrocyte differentiation of ASCs, using a GFP-Sox9 promoter vector screening system. An in vivo study was performed to confirm the recovery rate of cartilage regeneration with ASC differentiation into chondrocytes by ELPC in a collagenase-induced animal model of OA. Taken together, these data indicate that ellipticine induces ASCs to differentiate into mature chondrocytes without hypertrophic chondrocytes in vitro and in vivo, thus overcoming a problem encountered in previous studies. These results indicate that ELPC is a novel chondrocyte differentiationinducing drug that shows potential as a cell therapy for OA.
Seo, Hyang-Hee,Kim, Sang Woo,Lee, Chang Youn,Lim, Kyu Hee,Lee, Jiyun,Lim, Soyeon,Lee, Seahyoung,Hwang, Ki-Chul Elsevier 2017 european journal of pharmacology Vol.798 No.-
<P>Excessive vascular smooth muscle cell (VSMC) proliferation and migration after vascular injury significantly contributes to the development of occlusive vascular disease. Therefore, inhibiting the proliferation and migration of VSMCs is a validated therapeutic modality for occlusive vascular disease such as atherosclerosis and restenosis. In the present study, we screened chemical compounds for their anti -proliferative effects on VSMCs using multiple approaches, such as MTT assays, wound healing assays, and trans -well migration assays. Our data indicate that 7-cyclopenty1-5-(4-phenoxypheny1)-7H-pyrrolo[2,3-d] pyrimidin-4-ylamine, a lymphocyte -specific protein tyrosine kinase (Lck) inhibitor, significantly inhibited both VSMC proliferation and migration. 7-cyclopenty1-5-(4-phenoxypheny1)-7H-pyrrolo[2,3-d]pyrimidin-4-ylamine suppresses VSMC proliferation and migration via down -regulating the protein kinase B (Akt) and extracellular signal regulated kinase (ERK) pathways, and it significantly decreased the expression of proliferating cell nuclear antigen (PCNA) and cyclin Dl and, the phosphorylation of retinoblastoma protein (pRb). Additionally, 7-cyclopenty1-5-(4phenoxypheny1)-7H-pyrrolo[2,3-d] pyrimidin-4-ylamine suppressed the migration of VSMCs from endothelium -removed aortic rings, as well as neointima formation following rat carotid balloon injury. The present study identified 7-cyclopenty1-5-(4-phenoxypheny1)-7H-pyrrolo[2,3-d]pyrimidin-4-ylamine as a potent VSMC proliferation and migration inhibitor and warrants further studies to elucidate its more detailed molecular mechanisms, such as its primary target, and to further validate its in vivo efficacy as a therapeutic agent for pathologic vascular conditions, such as restenosis and atherosclerosis.</P>
Seo, Hyang-Hee,Kim, Sang Woo,Lee, Chang Youn,Lim, Kyu Hee,Lee, Jiyun,Choi, Eunhyun,Lim, Soyeon,Lee, Seahyoung,Hwang, Ki-Chul BioMed Central 2017 BIOLOGICAL RESEARCH Vol.50 No.-
<P><B>Background</B></P><P>Pathologic vascular smooth muscle cell (VSMC) proliferation and migration after vascular injury promotes the development of occlusive vascular disease. Therefore, an effective chemical agent to suppress aberrant proliferation and migration of VSMCs can be a potential therapeutic modality for occlusive vascular disease such as atherosclerosis and restenosis. To find an anti-proliferative chemical agent for VSMCs, we screened an in-house small molecule library, and the selected small molecule was further validated for its anti-proliferative effect on VSMCs using multiple approaches, such as cell proliferation assays, wound healing assays, transwell migration assays, and ex vivo aortic ring assay.</P><P><B>Results</B></P><P>Among 43 initially screened small molecule inhibitors of kinases that have no known anti-proliferative effect on VSMCs, a spleen tyrosine kinase (Syk) inhibitor (BAY61-3606) showed significant anti-proliferative effect on VSMCs. Further experiments indicated that BAY61 attenuated the VSMC proliferation in both concentration- and time-dependent manner, and it also significantly suppressed the migration of VSMCs as assessed by both wound healing assays and transwell assays. Additionally, BAY61 suppressed the sprouting of VSMCs from endothelium-removed aortic rings.</P><P><B>Conclusion</B></P><P>The present study identified a Syk kinase inhibitor as a potent VSMC proliferation and migration inhibitor and warrants further studies to elucidate its underlying molecular mechanisms, such as its primary target, and to validate its in vivo efficacy as a therapeutic agent for restenosis and atherosclerosis.</P>
Lee, Jiyun,Morton, Y.T. Jade,Lee, Jinsil,Moon, Hee-Seung,Seo, Jiwon IEEE 2017 IEEE signal processing magazine Vol.34 No.5
<P>The ionosphere has been the most challenging source of error to mitigate within the community of global navigation satellite system (GNSS)-based safety-critical systems. Users of those systems should be assured that the difference between an unknown true position and a system-derived position estimate is bounded with an extremely high degree of confidence. One of the major concerns for meeting this requirement, known as integrity, is ionosphere-induced error or discontinuity of GNSS signals significant enough to threaten the safety of users. The potentially hazardous ionospheric anomalies of interest in this article are ionospheric spatial decorrelation and ionospheric scintillation under disturbed conditions. As the demand of safety-critical navigation - applications increases with the rapid growth of the autonomous vehicle sector, ionospheric monitoring and mitigation techniques become more important to support such systems.</P>
Lee, Jiyun,Kwon, Gayeung,Park, Jieun,Kim, Jeong-Keun,Choe, Soo Young,Seo, Yoonhee,Lim, Young-Hee Informa UK (TaylorFrancis) 2016 Bioscience, Biotechnology, and Biochemistry Vol.80 No.7
<P>Inappropriate platelet aggregation can cause blood coagulation and thrombosis. In this study, the effect of an ethanol extract of Ramulus mori (ERM) on blood circulation was investigated. The antithrombotic activity of ERM on rat carotid arterial thrombosis was evaluated in vivo, and the effect of ERM on platelet aggregation and blood coagulation time was evaluated ex vivo. To evaluate the safety of ERM, its cytotoxicity to platelets and its effect on tail bleeding time were assessed; ERM was not toxic to rat platelets and did not prolong bleeding time. Moreover, administering ERM to rats had a significant preventive effect on carotid arterial thrombosis in vivo, and significantly inhibited adenosine diphosphate- and collagen-induced platelet aggregation ex vivo, whereas it did not prolong coagulation periods, such as prothrombin time and activated partial thromboplastin time. The results suggest that ERM is effective in improving blood circulation via antiplatelet activity rather than anticoagulation activity.</P>
Yun-Seo Lee,Jae Jun Lee,Dongkyun Gil,Jiyun Kang,Ki-Tae Kim,Cheal Kim 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-
Due to the twin-demic of COVID-19 and flu virus, disinfectants containing ClO- have been widely usednowadays. Therefore, it is urgent to develop a sensor capable of efficiently detecting toxic hypochlorite. We present the invention and assessment of a fast-responsive and multi-applicable chemodosimeter sensorETA (2-(2-((1E,2E)-3-(4-(dimethylamino)phenyl)allylidene)hydrazineyl)-N,N,N-trimethyl-2-oxoethan-1-aminium chloride) for monitoring ClO . In pure water, adding ClO- to ETA caused a turn-offfluorescence within 2 sec. These changes made it possible to quickly detect ClO- with a high level of selectivity. ETA displayed a low detection limit (0.68 lM) to ClO-. Using UV–vis titrations, ESI-MS and DFT calculations,we were able to demonstrate the detection mechanism, in which ETA was cleaved by ClO-. Inparticular, we established the possibility for reliable ClO- detection in environmental systems such asactual water samples, disinfectants, living cells, zebrafish and celery, in addition to confirming the practicalityof ETA utilizing test strips.
Human Long Noncoding RNA Regulation of Stem Cell Potency and Differentiation
Lee, Seahyoung,Seo, Hyang-Hee,Lee, Chang Youn,Lee, Jiyun,Shin, Sunhye,Kim, Sang Woo,Lim, Soyeon,Hwang, Ki-Chul Hindawi 2017 Stem cells international Vol.2017 No.-
<P>Because of their capability of differentiation into lineage-specific cells, stem cells are an attractive therapeutic modality in regenerative medicine. To develop an effective stem cell-based therapeutic strategy with predictable results, deeper understanding of the underlying molecular mechanisms of stem cell differentiation and/or pluripotency maintenance is required. Thus, reviewing the key factors involved in the transcriptional and epigenetic regulation of stem cell differentiation and maintenance is important. Accumulating data indicate that long noncoding RNAs (lncRNAs) mediate numerous biological processes, including stem cell differentiation and maintenance. Here, we review recent findings on the human lncRNA regulation of stem cell potency and differentiation. Although the clinical implication of these lncRNAs is only beginning to be elucidated, it is anticipated that lncRNAs will become important therapeutic targets in the near future.</P>