아세톤-물 혼합용매중의 브롬화 펜아실과 치환 아닐린과의 반응속도

홍성완,박진하 又石大學校 1983 論文集 Vol.5 No.-

The rate constants of the reactions of phenacyl bromide with substituted anilines in acetone-water were conductometrically determined, and the activation parameters were also evaluated. In results, the rate constants were first order with respect to each reactant and increased as higher water consent and in the other of substituents as p-Cl<H<p-CH_3. It was concluded that the reactions were to be S_N2 mechanisms. Ham mett and Broensted relationships were considered too.

向精神藥物과 蟲垂炎 및 麻痺性 腸閉塞에 관한 臨床的 考察

禹幸源,田珍淑 梨花女子大學校 韓國生活科學硏究院 1982 韓國生活科學硏究院 論叢 Vol.30 No.-

본연구는 임상에서 향정신약물요법을 시행하는 중 이의 항콜린효과(anticholinergic effect)로 인해 병발한 복부증상중 충수염과 마비성장폐색에 관해 연구하였다. 정신과 환자들은 질환자체증상인 무관심과 치료약물의 동통완화작용으로 인해 증상호소력이 경미하므로 흔히 진단과 치료의 지연을 초래하게 되고 사망에까지 이를 수 있는 잠재적 위험을 내포하고 있다. 이에 저자는 즉각적인 외과적 치료가 요구되는 향정신약물로 인한 복부 부작용의 증례를 검토하여 이를 조기발견하여 조기치료함으로써 향정신약물로 인한 복부 부작용 증상을 효과적으로 치료 및 예방하는데 주안점을 두어 본 연구를 시도하였다. 저자는 1977년 1월 1일부터 1981년 12월 31일까지 이화여자대학교 부속병원 신경정신과에 입원하여 향정신약물로 치료받은 997예중 충수염과 마비성장폐색이 발병된 26예에 대해서 병록검토 및 문헌고찰을 한 결과 다음과 같은 결론을 얻었다. 1. 충수염과 마비성장폐색의 발생은 20대가 13예(53.8%)로서 제일 많았다. 2. 정신과적 진단명은 정신분열병이 14예(53.8%)로서 제일 많았다. 3. 입원횟수와 입원전 약물치료 경험에 있어서는 충수염환자는 1회 입원이 7예(50.0%)로서 제일 많았고 마비성장폐색은 2회 입원이 5예(41.7%)로서 제일 많았고 12예중 9예(75.0%)가 입원전약물 치료 경험이 있었다. 4. 투약에 있어서는 1예(3.8%)는 항정신병제를 단독사용하였고, 25예(96.2%)는 항정신병제, 항우울제, 항파킨손제를 복합사용하였는데 25예중15예(57.7%)는 항정신병제 중에서도 두종류 이상의 phenothiazine과 항파킨손제제가 복합사용되었다. 약물의 용량은 충수염이 마비성장폐색보다 일반적으로 많았다. 5. 발병시기에 있어서는 3∼5주가 10예(38.5%)로서 제일 많았는데 충수염은 입원 1∼3주에 14예중 6예(42.9%)가 발병했고, 마비성장폐색은 12예중 7예(58.4%)가 3∼5주에 발병했다. 6. 임상증상은 충수염에서는 우하복부동통과 오심, 구토가 10예로서 제일 많았고, 마비성장폐색에서는 복부팽만이 9예로서 제일 많았고 설사 변비가 7예였다. 7. 혈액소견은 정상범위가 17예로서 제일 많았고 복부 방사선소견은 충수염은 가스팽창이 13예로서 제일 많았고, 마비성장폐색은 전예에서 가스팽창과 그 중 6예에서 air-fluid level을 보였다. 충수염 환자 중 충수절제술을 받은 11예 중에서 10예는 분석을 충수강 내용물로 함유했다. 8. 충수염 환자는 11예(78.6%)에서 수술로서 치유되었고 마비성 장폐색 환자는 전예가 보존요법으로 치유되었다. This study was made to appreciate the potential fatality of the abdominal complications caused by the anticholinergic side effect of psychotropic drugs and to suggest the possible ways of early diagnosis, treatment and prevention in concerned with those complications. The 26 cases of appendicitis and paralytic ileus among 997 casesof psychiatric in-patients admitted in Ewha Womans University Hospital from January 1st in 1977 to December 31st in 1981 were retrospectively reviewed and discussed. The following results were obtained: 1. The age distribution of appendicitis and paralytic ileus showed peak in twenties (50.0%). 2. The most frequent psychiatric diagnosis was schizophrenic disorder (53.8%). 3. 7 Cases (50.0%) of appendicitis occurred in the 1st admission, and 5 Cases (41.7%) of paralytic ileus occurred in the 2nd admission. On the previous history of pharmacotherapy, there were 7 cases (50.0%) in appendicitis and 9 cases (75.0%) in paralytic ileus. 4. 25case (96.2%) were prescribed with polypharmacy, 15 cases (57.7%) were combination of over 2 kinds of phenothiazines with antiparkinsonian drugs. The dosage was larger in appendicitis than in paralytic ileus. 5. Most frequent time of onset of appendicitis and paralytic ileus was at 3-5 wks after admission (38.5%). 6. The clinical symptoms frequently seen were abdominal distention (9 cases), diarrhea or constipation (7 cases) in paralytic ileus, while right lower abdominal pain, nausea and vomiting were most frequently seen in appendicitis (10 cases). 7. Hematological findings showed within normal limit in 17 cases. The abdominal X-ray showed gaseous distention in 25 cases, air-fluid level in 8 cases and fecal mass in 4 cases. The content of the appendix was filled with fecalith in 10 cases among 11 appendicectomized cases. 8. Most of patients with appendicitis were cured by appendectomy (78.6%), and all the patients with paralytic lieus were healed by various supportive measures.

아세톤-물 混合溶媒中의 브롬化페나실과 파라클로아닐린과의 反應速度硏究

朴鎭夏,洪性完 全北大學校 基礎科學硏究所 1982 基礎科學 Vol.5 No.1

The rate constants of the reactions of phenacyl bromide with p-chloroaniline in acetone-water at 30, 35 and 40℃ were determined by means of electric conductivity method and some activation parameters were also calculated. In the results, the rate constants were first order with respect to each reactant and increased with water content and the values of entropy of activation were quite negative and those of enthalpy of activation were comparatively low. Therefore, it is concluded that the reactions were to be S_N2 mechanism.

면역방사계수법을 이용한 Thyroglobulin 측정시 항 Thyroglobulin 항체의 존재가 미치는 영향

안병철,배진호,정신영,박호용,김정국,하승우,이재태,김보완,이규보 대한내분비학회 2004 Endocrinology and metabolism Vol.19 No.1

연구배경: 혈청 thyroglobulin (Tg)은 옥소전신스캔과 함께 분화갑상선암환자에서 갑상선암 재발에 대한 추적관찰에 매우 예민하고, 중요한 표지자로 임상에서 널리 이용된다. 그러나 많은 수의 분화갑상선암환자는 Tg 항체인 항 Tg 항체를 가지고 있고, 이는 방사면역검사법 (radioimmunoassay) 및 면역방사계수 검사법(immunoradiometric assay)으로 Tg 측정시 영향을 미칠 수 있다. 이에 연자들은 면역방사계수법으로 Tg를 측정할 경우, 항 Tg항체에 의하여 어떤 영향이 생길수 있는지를 알아보고자 하였다. 방법: 이중위치 고상법 (solid phase two-site)법을 이용하는 ELSA-hTg in vivo test (CIS international, Schering, France) 시약을 이용하여 Tg를 측정하였다. Tg과 항 Tg 항체는 검사시약에 포함된 표준용액을 사용하였으며, Tg는 두 가지 농도 (23.5 ng/mL, 62.5 ng/mL)로, 항 thyroglobulin 항체는 세 가지 농도(25U/mL, 50U/mL, 100U/mL)로 이용하였다. Tg가 높게 나타나는 환자의 혈청에 항 Tg 항체가 높게 나타나는 환자의 혈청을 혼합하여 동일한 방법으로 Tg 검사를 시행하였다. 통계학적 분석은 ANOVA와 Scheffe test 및 Quadratic regression modeling을 시행하였다. 결과: 면역방사계수법을 이용한 Tg 측정시 항 thyroglobulin 항체가 존재하는 경우 그 값이 낮게 측정되었다. Tg 농도가 23.5 ng/mL인 표준용액이 항 Tg 항체의 농도가 0, 25, 50 및 100U/mL 경우, 각각 24.5±1.1, 11.8±0.4, 7.7±0.1 및 4.5±0.4ng/mL로 측정되었으며, Tg 농도가 62.5 ng/mL인 표준용액은 각각 65.9±5.7, 36.3±2.2, 23.7±0.7, 및 14.0±1.0 ng/mL로 측정되었다(ANOVA test, p=0.000). 항 Tg 항체에 의한 Tg 측정값의 저하 정도는 항 Tg 항체의 농도에 비례하는 것으로 나타났다 (Quadratic model regression, SigT=0.000). 결론: 분화갑상선암환자에서 항 Tg 항체의 존재는 면역방사계수법을 이용한 혈청 Tg 측정으로 갑상선암 재발을 파악하는데 방해하는 인자로 작용될 수 있으며, Tg 측정시 항 Tg 항체의 측정이 꼭 필요하며, 항 Tg 항체를 가진 분화갑상선암환자에서 Tg 수치를 해석할 경우 세심한 주의가 요구된다. Background: Serum thyroglobulin (Tg) is a valuable and sensitive tool needed in the follow-up of patients with differentiated thyroid cancer (DTC), but antithyroglobulin antibody (Anti-Tg), common in patients with DTC, can interfere with the assay for Tg. In this study, we evaluated the influence of Anti-Tg on the measurement of Tg using the immunoradiometric assay (IRMA). Methods: In using ELSA-hTg in vivo test (CIS international, Schering, France), a solid phase two-site IRMA was used to measure Tg (23.5 ng/mL, 62.5 ng/mL) under the absence or presence of three concentrations of Anti-Tg (25U/mL, 50U/mL, 100U/mL). We also performed Tg measurement using patients serum that was mixed with patients serum containing high Anti-Tg. ANOVA and Scheffe tests were performed to evaluate the effect of Anti-Tg on Tg IRMA, and an inverse regression was made to calculate the level of Tg from measured Tg and used Anti-Tg levels and also to assess the degree of effect of anti-Tg on Tg IRMA. Results: In measuring Tg using the standard solution, the presence of Anti-Tg resulted in a falsely suppressed Tg value. The IRMAs for 23.5ng/mL of the standard Tg solution resulted in 24.5±1.1 ng/mL under no Anti-Tg, 11.8±0.4 ng/mL under 25U/mL of Anti-Tg, 7.7±0.1 ng/mL under 50U/mL of Anti-Tg, and 4.5±0.4 ng/mL under 100U/mL of Anti-Tg. IRMAs 62.5 ng/mL of the standard Tg solution resulted in 65.9±5.7 ng/mL under no Anti-Tg, 36.3±2.2 ng/mL under 25U/mL of Anti-Tg, 23.7±0.7 ng/mL under 50U/mL of Anti-Tg, and 14.0±1.0 ng/mL under 100U/mL of Anti-Tg. (ANOVA test, p=0.000). The degree of suppression of the measured Tg value was positively correlated with the Anti-Tg level (Quadratic model regression, Sig T=0.000). The presence of Anti-Tg also resulted in a falsely suppressed Tg value for the Tg measurement using patient's serum. 2004). Conclusion: The presence of Anti-Tg could consist of the use of Tg as a tumor, therefore Anti-Tg should be measured in all patients diagnosed with DTC. The interpretation of the Tg level must be performed with extreme caution in patients with Anti-Tg (J Kor Soc Endocrinol 19:42∼47, 2004)

Quality Characteristics and Antioxidative Activity of Bitter Melon (Momordica charanita L.)

Ha Jeong BOO,You Jin PARK,Jung Won CHOI,Ji Wan KO,Su Yeon KIM,You Jin NAM,Hee Jin SEOL,Agriculture Association Corpra,Ji-Yeon CHUN 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.10

Enzymatic transformation of platycosides and one-step separation of platycodin D by high-speed countercurrent chromatography

Ha, In Jin,Ha, Young Wan,Kang, Minseok,Lee, Jongsung,Park, Deokhoon,Kim, Yeong Shik WILEY-VCH Verlag 2010 Journal of Separation Science Vol.33 No.13

<P>Platycosides, the saponins found in the roots of Platycodon grandiflorum (Platycodi Radix), are typically composed of oleanane triterpenes with two side chains. In platycosides, platycodin D, a glucose unit at C-3, is a major component, which has several pharmacological activities. Because of the high demand for this compound, we attempted to enzymatically convert platycodin D<SUB>3</SUB> and platycoside E, having two and three glucose units at C-3, respectively, into platycodin D. In this study, we tested the ability of several glycosidases to transform platycosides, or more specifically, the ability to transform platycoside E and platycodin D<SUB>3</SUB> into platycodin D. To obtain pure platycodin D on a preparative scale, high-speed countercurrent chromatography with a solvent system of ethyl acetate/n-butanol/water (1.2:1:2, v/v/v) was used for the separation of the enzymatically transformed product. Approximately 39.4 mg of platycodin D (99.8% purity) was obtained from 200 mg of the product in a one-step separation. The results strongly support the advantage of enzymatic transformation of the platycosides for the efficient enrichment of platycodin D in the complicated extract of the medicinal plant.</P>

Enzymatic transformation of platycosides and one-step separation of platycodin D by high-speed counter-current chromatography

In Jin Ha,Young Wan Ha,Minseok Kang,Jongsung Lee,Deokhoon Park,Yeong Shik Kim 한국당과학회 2010 한국당과학회 학술대회 Vol.2010 No.1

Platycosides, the saponins found in the roots of Platycodon grandiflorum (PlatycodiRadix), are typically composed of oleanene backbones with two side chains. Of the platycosides, platycodin D (glucose unit at C-3) is a major component and it has several pharmacological activities. Because of the high demand for this compound, we attempted to enzymatically covert platycodin D3 and platycoside E, having two and three glucose units at C-3, respectively, into platycodinD. In this study, we tested the ability of several glycosidases to transform platycosides, or morespecifically, the ability to transform platycoside E and platycodin D3 into platycodin D. To get pure platycodin D on a preparative scale, high-speed counter-current chromatography (HSCCC) with a solvent system ethyl acetae-n-butanol-water (1.2:1:2, v/v/v) was used for separation of the enzymatically-transformed product from the reaction mixture. 40.5 mg of platycodin D (99.8% purity) was obtained from 200 mg of the productin one-step seapartion.

Preparative Isolation of Ginsenosides from Steam-treated Panax Ginseng C. A. Meyer) by High-speed Counter-current Chromatography Coupled with an Evaporative Light Scattering Detector

In Jin Ha(하인진),Young Wan Ha,Heungsop Shin,Yeong Shik Kim 고려인삼학회 2009 고려인삼학회 학술대회 Vol.2009 No.5

탄성체가 적용된 점탄성 유체 댐퍼의 주파수 의존성에 대한 실험적 연구

이완하(Wan-ha Lee),박진영(Jin-young Park),조현진(Hyun-jin Cho),오주(Ju Oh),박건록(Kun-nok Park) 한국소음진동공학회 2009 한국소음진동공학회 학술대회논문집 Vol.2009 No.4

손상해석을 이용한 CF8A 열화재의 파괴저항성 예측 기법

황진하(Jin-Ha Hwang),류호완(Ho-Wan Ryu),김윤재(Yun-Jae Kim),김진원(Jin-Won Kim) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.4