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Gui Fang-Fang,Jiang Ge-Ge,Bin Dong,Zhong Shi-Wei,Xiao Zheng,Qiu Fang,Wang Yi-Guang,Yang Li-Yuan,Zhao Hongbo 한국유전학회 2023 Genes & Genomics Vol.45 No.9
Background MIKC type MADS-box transcription factors are one of the largest gene families and play a pivotal role in flowering time and flower development. Chimonanthus salicifolius belongs to the family Calycanthaceae and has a unique flowering time and flowering morphology compared to other Chimonanthus species, but the research on MIKC type MADS-box gene family of C. salicifolius has not been reported. Objective Identification, comprehensive bioinformatic analysis, the expression pattern of MIKC-type MADS-box gene family from different tissues of C. salicifolius. Methods Genome-wide investigation and expression pattern under different tissues of the MIKC-type MADS-box gene family in C. salicifolius, and their phylogenetic relationships, evolutionary characteristics, gene structure, motif distribution, promoter cis-acting element were performed. Results A total of 29 MIKC-type MADS-box genes were identified from the whole genome sequencing. Interspecies synteny analysis revealed more significant collinearity between C. salicifolius and the magnoliids species compared to eudicots and monocots. MIKC-type MADS-box genes from the same subfamily share similar distribution patterns, gene structure, and expression patterns. Compared with Arabidopsis thaliana, Nymphaea colorata, and Chimonanthus praecox, the FLC genes were absent in C. salicifolius, while the AGL6 subfamily was expanded in C. salicifolius. The selectively expanded promoter (AGL6) and lack of repressor (FLC) genes may explain the earlier flowering in C. salicifolius. The loss of the AP3 homologous gene in C. salicifolius is probably the primary cause of the morphological distinction between C. salicifolius and C. praecox. The csAGL6a gene is specifically expressed in the flowering process and indicates the potential function of promoting flowering. Conclusion This study offers a genome-wide identification and expression profiling of the MIKC-types MADS-box genes in the C. salicifolius, and establishes the foundation for screening flowering development genes and understanding the potential function of the MIKC-types MADS-box genes in the C. salicifolius.
Dong Fang,Zheng Hu-Zhe,Jeong Woo-Sik,Chung Shin-Kyo,Qu Zhong-Yuan,Zou Xiang,Liu Chen,Xiang Qiong,Feng Feng 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.S
In order to obtain and explore selenide composed of selenium and polysaccharide, three parameters were selected to optimize the synthesis process of selenium-Euryale ferox Salisb. polysaccharide (Se-ESPS) by Box-Behnken design. Furthermore, ESPS-B1 separated from ESPS was selenitized to Se-ESPS-B1 by the optimal synthesis process, then the characterization, and antioxidant activity in vitro of Se-ESPS-B1 were explored. The result manifested that the Se content of Se-ESPS was (2.915 ± 0.03) mg/g according to the optimal synthesis process of Se-ESPS (reaction time at 5 h, reaction temperature at 81 ℃, weight ratio of Na2SeO3 to ESPS at 0.9 g/g). A series of detection results indicated that the characterizations of Se-ESPS-B1 were apparently distinguished from that of ESPS-B1. Moreover, the antioxidant experiments in vitro demonstrated that Se-ESPS-B1 could exert antioxidant activity by scavenging DPPH, ABTS,·OH, and increasing reduction ability. In conclusion, the synthesis process is an effective approach to harvest seleniumpolysaccharide, and Se-ESPS-B1 may be utilized as a potential antioxidant or selenium nutritional supplement.
Fang-dong Li,Yan-zhi Feng,Lu Wang,Jian-min Fu,Ta-na Wuyun,Hong-yan Du,Xiao-feng Tan,Feng Zou 한국유전학회 2016 Genes & Genomics Vol.38 No.3
Eucommia ulmoides is a focus of considerable attention because it is used to produce gutta-percha latex and raw materials for traditional Chinese medicine. The species has also been widely planted for vegetable oil production due to the high content of alpha-linolenic acid (ALA) in the seeds. However, little was known about fatty acid biosynthesis in the seeds. Here, we present transcriptome analyses that identified genes related to the fatty acid biosynthesis pathways in the seeds collected 70 and 160 days after flowering (DAF) from two varieties: ‘Huazhong No. 6’ (ALA content of 38.7 %) and ‘Huazhong No. 10’ (ALA content of 67.6 %). De novo assembly generated 96,469 unigenes with an average length of 690 bp. Among them, 11,260 unigenes identified by functional annotation against the Kyoto Encyclopedia of Genes and Genomes database were mapped to 117 metabolic pathways. 65 unigenes involved in fatty acid biosynthesis include 5 coding for 3-oxoacyl-ACP reductase, 2 coding for b-hydroxyacyl-ACP dehydratase, 10 coding for b-ketoacyl-ACP synthase II, and 2 coding for enoyl-ACP reductase I. Expression of the nine differentially expressed unigenes coding for major enzymes in fatty acid biosynthesis was validated by real-time fluorescent quantitative PCR (qPCR) using RNA from six seed stages (70, 88, 106, 124, 142, and 160 DAF) in ‘Huazhong No. 10’. The qPCR analysis showed that expression of these 9 unigenes all changed significantly at 88 DAF. This study provides a large-scale transcriptome annotation of E. ulmoides, which is fundamental information for further characterizing gene functionals involved in the process of seed development.
Dong Dong Zhang,Yang Fang Wu,Wei Xia Chen,Yao Xu,Si Yan Liu,Huang Huang Luo,Guang Mei Jiang,Yue Wu,Peng Hu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Renal osteodystrophy (ROD) occurs as early as chronic kidney disease (CKD) stage 2 and seems ubiquitous in almost all pediatric patients with CKD stage 5. Fibroblast growth factor (FGF)-23, a bone-derived endocrine regulator of phosphate homeostasis, is overexpressed in CKD and disturbs osteoblast differentiation and matrix mineralization. In contrast, C-type natriuretic peptide (CNP) acts as a potent positive regulator of bone growth. In the present study, we infused CNP into uremic rats and observed whether CNP could attenuate ROD through the inhibition of FGF-23 cascades. In uremic rats, CNP administration significantly alleviated renal dysfunction, calcium phosphate metabolic disorders, hypovitaminosis D, secondary hyperparathyroidism, the decrease in bone turnover markers and retarded bone pathological progression. More importantly, within FGF-23/mitogen-activated protein kinase (MAPK) signaling, the fibroblast growth factor receptor-1, Klotho and alternative (STAT-1/phospho-STAT-1) elements were upregulated by CNP, whereas FGF-23, RAF-1/phospho-RAF-1, and downstream (ERK/phospho-ERK and P38/phospho-P38) elements were paradoxically underexpressed in bone tissue. Therefore, CNP exerts a therapeutic effect on ROD through inhibition of FGF-23/MAPK signaling at the RAF-1 level.