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정호철,이상한,정신교 慶北大學校農業科學技術硏究所 2007 慶北大農學誌 Vol.25 No.-
The several enzymes reaction which involve the quality change of fresh vegetables, such as chlorophylase, polyphenol oxidase, lypoxygenase, C-S-Iyase, myrosinase and enzymes related lignification were reviewed. Numerous enzyme reaction continuously proceeds to the deterioration of vegetables after harvest due to the respiration and biochemical metabolism reaction, especially in case of physical injuring. It is extremely important to inhibit and to control these enzyme reaction in order to maintain the organoleptic and nutritional quality of fresh vegetables.
Hu-Zhe Zheng,Sun-Young Kwon,Shin-Kyo Chung 한국응용생명화학회 2020 Journal of Applied Biological Chemistry (J. Appl. Vol.63 No.3
In order to enhance the extraction efficiency of flavonoid from Saururus chinensis, carbohydrate-hydrolyzing enzyme Viscozyme L aided extraction techniques have been studied. Then flavonoid composition, as well as quercetin, were also identified using UV/Vis, HPLC/MS, and 1H-NMR. The results showed that favorable extraction conditions were Viscozyme L concentration of 0.25 mg/g, pH 4.2, reaction at 45 oC for 12 h. Under the favorable extraction condition, total flavonoid yield (37.9 mg/g) and quercetin yield (0.86 mg/g) increased by about 2.0 and 9.6 times, respectively, compared to control group. Interestingly, as a significant flavonoid of S. chinensis, flavonoid glycones rutin was hydrolyzed to aglycones quercetin by Viscozyme L. These findings provide scientific and theoretical support for the development quercetin-rich products, which was quickly absorbed by the human body than rutin.
Hu Zhe Zheng,In Wook Hwang,Suk Kyung Kim,Sang Han Lee,Shin Kyo Chung 한국응용생명화학회 2010 Journal of Applied Biological Chemistry (J. Appl. Vol.53 No.3
Unripe apples lie scattered about the orchards because of manual thinning out or falling, however they contain a high amount of polyphenol. In order to enhance the extraction of polyphenol from the unripe apples, carbohydrate-hydrolyzing enzyme (Viscozyme
Optimization of Carbohydrate-hydrolyzing Enzyme Aided Polyphenol Extraction from Unripe Apples
Zheng, Hu-Zhe,Hwang, In-Wook,Kim, Suk-Kyung,Lee, Sang-Han,Chung, Shin-Kyo The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.3
Unripe apples lie scattered about the orchards because of manual thinning out or falling, however they contain a high amount of polyphenol. In order to enhance the extraction of polyphenol from the unripe apples, carbohydrate-hydrolyzing enzyme (Viscozyme L) aided polyphenol extraction techniques have been studied with response surface methodology. The optimum conditions were as follows: the ratio of Viscozyme L to substrate was 0.0195 (1.95 fungal beta-glucanase units), the reaction temperature was $47.12^{\circ}C$, and the reaction time was 12.52 h. The experimental values of total phenolic content and caffeic acid content of the unripe apples were well matched with the response surface methodology predicted values. The levels of total phenolic content and caffeic acid content obtained from Viscozyme L treatment were about 2 and 13 folds greater, respectively than those of the control treatment.
Phenolics Enrichment Process from Unripe Apples
Hu-Zhe Zheng,황인욱,김병관,김영찬,정신교 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.4
Unripe apples contain significant amount of phenolicswith various health benefits. A pilot scale enrichment process ofunripe apple phenolics with Viscozyme L extraction and XAD-7sorption process, based on the total phenolic content (TPC),phenolics content by high-performance liquid chromatographyanalyses, and antioxidant activities were studied. Antioxidantactivities were tested by measuring oxygen radical absorbingcapacity (ORAC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicalscavenging activity, and ferric-reducing antioxidant power(FRAP). The phenolics-rich final product, apple antioxidantphenolics (AAP) has 771-fold higher TPC and 600-fold higherantioxidant activity of unripe apples. Through XAD-7 sorptionAAP showed 52, 87, 70 and 44-fold increases in TPC, ORAC,DPPH and FRAP values, respectively. AAP showed more than95% stability at the temperature range of 20 to 120°… and pHrange of 1.47 to 8.5. Apple phenolics extraction process ofViscozyme L extraction coupled with XAD-7 sorption could beapplied to the production of health benefit antioxidants.
Zheng Hu-Zhe,Cui Chun-Lan,Jeong Woo-Sik,Chung Shin-Kyo 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.5
In order to improve the synergistic effect of unripe apple polyphenols (APP) and chitooligosaccharides (COS), apple polyphenols-chitooligosaccharides microcapsule (APCM) were prepared by spray-drying method. The effects of APCM on the release of polyphenols in simulated gastrointestinal digestion model, as well as the anti-inflammatory effect against LPS-induced RAW264.7 cells were also evaluated. Scanning electron microscope (SEM) and HPLC analysis of APP and APCM showed that during the spray-drying process, most of the polyphenols are successfully encapsulated in COS. The simulated gastrointestinal digestion model results showed that about 98% of polyphenols released from APCM within 60 min. Anti-inflammatory effect of APCM on LPS induced RAW 264.7 cells showed that although APP showed a strong inhibitory effect on cell viability at 0.6 mg/mL, the effect of APCM on cell viability was less and could maintain a high level at the same concentration. In addition, APCM significantly inhibited nitric oxide (NO) and TNF-α production via the elevation of cytokine IL-10 as the concentration increases, respectively. The results suggest that APCM alleviate the intensity of inflammatory processes by inhibiting the production of pro-inflammatory cytokines TNF-α, as well as additionally by promoting the production of anti-inflammatory cytokines IL-10. These findings provide scientific and theoretical support for the claim that traditional medicine treats inflammation-related diseases.