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Biomimetic Models of Heme and Nonheme Dioxygen Activating Enzymes
서유미 이화여자대학교 대학원 2007 국내석사
Synthetic modeling studies of high-valent iron(IV)-oxo intermediates of heme and nonheme iron monooxygenases have attracted much attention in the fields of bioinorganic and biological chemistry in recent years. Moreover, recent reports on the generation and characterization of mononuclear nonheme oxoiron(IV) complexes became a milestone in biomimetic studies of nonheme iron enzymes. In the present work, we performed parallel mechanistic studies on alcohol oxidation with in situ-generated high-valent iron-oxo complexes bearing heme and nonheme ligands. To the best of our knowledge, this study provides (1) the first example of alcohol oxidation by mononuclear nonheme oxoiron(IV) intermediates, (2) the first mechanistic details of alcohol oxidation investigated with in situ-generated heme and nonheme oxoiron(IV) complexes, and (3) the direct reactivity comparison of heme and nonheme oxoiron(IV) complexes in alcohol oxidation. Mononuclear nonheme oxoiron(IV) complexes bearing 15-membered macrocyclic ligands were generated from the reactions of their corresponding iron(II) complexes and iodosylbenzene (PhIO) in CH3CN. The oxoiron(IV) species were characterized with various spectroscopic techniques such as UV-vis spectrophotometer, electron paramagnetic resonance, electrospray ionization mass spectrometer, and resonance Raman spectroscopy. The oxoiron(IV) complexes were inactive in olefin epoxidation. In contrast, when iron(II) or oxoiron(IV) complexes were combined with PhIO in the presence of olefins, high yields of epoxide products were obtained. These results indicate that in addition to the oxoiron(IV) species, there must be at least one more active oxidant (e.g., FeIV-OIPh adduct or oxoiron(V) species) that effects the olefin epoxidation. We have also demonstrated that the ligand environment of iron catalysts is an important factor in controlling the catalytic activity as well as the product selectivity in the epoxidation of olefins by PhIO. Part I Heme과 nonheme iron monooxygenases의 synthetic modeling 연구는 최근 생무기와 생화학 분야에서 많은 흥미를 끌어왔다. 더욱이 mononuclear nonheme oxoiron(IV) 종의 생성과 특성에 대한 최근의 연구 결과는 nonheme iron enzyme의 생체 모방 연구 분야의 이정표가 되었다. 이 연구에서는 heme과 nonheme ligand를 가진 high-valent iron-oxo 종에 의한 alcohol oxidation mechanism에 대한 연구를 수행하였다. 이번 연구를 통해서 mononuclear nonheme oxoiron(IV) 종에 의한 alcohol oxidation의 첫 번째 예를 보여주었으며 최초로 in situ에서 생성한 heme과 nonheme ligand의 high-valent iron-oxo 종을 가지고 직접 alcohol oxidation의 mechanism에 대해 연구하였다. 또한 heme과 nonheme oxoiron(IV) 종에 의한 alcohol oxidation의 반응성을 직접적으로 비교해 볼 수 있었으며 heme과 nonheme oxoiron(IV) intermediate에 의한 alcohol oxidation이 α-CH hydrogen abstraction에 뒤이어 일어나는 electron-transfer process를 통해 일어나는 것으로 mechanism을 제안하였다 Part II 15-membered macrocyclic ligand를 가진 iron(II) 화합물들과iodosylbenzene (PhIO)을 CH3CN에서 반응시켜 새로운 mononuclear nonheme oxoiron(IV) 종을 생성하였다. 이렇게 새로이 생성시킨 oxoiron(IV) 종을 UV-vis spectrophotometer, electron paramagnetic resonance, electrospray ionization mass spectrometer 및 resonance Raman spectroscopy 와 같은 다양한 분광학적 방법을 통해 특성을 규명하였다. 이 oxoiron(IV) 종은 olefin epoxidation 반응에서 직접적으로 반응성을 보이지 않았으나, olefin 존재 하에 iron(II) 또는 oxoiron(IV) 종이 PhIO와 결합하면 높은 수율의 epoxide product가 얻어짐을 알 수 있었다. 이 결과들은 olefin epoxidation 반응에서 oxoiron(IV) 종 외에 반응성을 가지는 또 다른(최소한 하나이상의), 보다 강력한 산화종 ( FeIV-OIPh adduct 또는 oxoiron(V) )이 존재함을 암시한다. 또한 이 연구에서는 철 화합물의 ligand environment가 PhIO에 의한 olefin epoxidation 반응에 있어서 catalytic activity 와 product selectivity를 조절하는 중요한 요소임을 보였다.
골반통증과 마이코플라즈마 제니탈리움 : 골반통증과 마이코플라즈마 제니탈리움 감염의 상관관계
서유미 단국대학교 대학원(천) 2024 국내박사
골반통증과 마이코플라즈마 제니탈리움 단국대학교 대학원 의학과 비뇨의학전공 서 유 미 지도교수: 이 길 호 목적 현재 마이코플라스마 제니탈리움 (Mycoplasma genitalium; M. genitalium)이 만성전립 선염 혹은 골반 통증에 원인 인가에는 많은 논란이 있어 왔다. 따라서 저자는 오줌 에 M. genitalium감염이 만성전립선염 혹은 골반통 발현과의 상관관계를 조사하고, 증례-대조 연구 (case-control study)를 통해 M. genitalium에 의한 골반통의 특징적인 소견을 알기 위해 본 연구를 시작하였다. 재료 및 방법 1. 2009년~2022년 동안 단국대학교병원 비뇨의학과에 내원한 환자 중 유전자증폭법 등으로 소변에 M. genitalium 단독감염이 확인된 113명의 남성을 대상으로 국립보건 원-만성전립선염 증상표 (Korean version of NIH-Chronic Prostatitis Symptom Index; CPSI)을 이용해 감염 환자의 골반 통증의 존재와 그 특징을 조사하였다. 2. 이들 검체에서 시행한 항생제 내성 결과에 따라 azithromycin 이나 moxifloxacin 같은 항생제 치료 후 시행한 완치검사 (Test of cure: TOC)에서 그 결과를 확인할 수 있었던 41명을 대상으로 완치군 27명과 감염 지속군 14명으로 분류하고 이들을 대 상으로 치료 전/ 치료 후 CPSI 설문지 점수 변화를 비교해 M. genitalium의 치료 성 공에 따른 골반통 증상변화 차이를 관찰하였다. 3. 113명 중 급성요도염 증상이나 증후가 있었던 33명을 제외해 만성전립선염 혹은 만성 골반통증증후군 의심 환자 80명과 이들과 증상과 연령 분포가 유사한 234명의 M. genitalium, Chlamydia trachomatis, Neisseria gonorrhoeae 감염 음성 대조군으로 증 례/대조 연구를 시행해 M. genitalium 감염의 특징적인 골반통을 조사하였다. 결과 1. 113명 환자는 CPSI 설문지 증상 항목에서 회음부 통증 (1a) 25.7%, 고환통증 (1b) 21.2%, 음경 통증 (1c) 31%, 하복부 통증 (1d) 18.6%를 보여 M. genitalium에 감염된 많은 환자가 골반 통증을 호소하였다. 배뇨 통증 (2a)은 59.3%, 사정통 (2b)은 23%에 서 관찰되어 환자의 반 이상이 요도 배농과 관계없는 배뇨 통증을 호소하였다. CPSI 설문지에 통증 영역 및 배뇨 영역의 합, 총점은 각각 6.68 ± 4.75, 2.69 ± 2.66, 15.00 ± 8.66으로 M. genitalium 에 감염되면 임상적으로도 의미 있는 골반통 증이 관찰되었다. 2. 성공적인 항생제 치료는 CPSI 설문지 증상 항목 중 요저장 능력 (item-6)을 제외 한 모든 항목에서 증상이 호전되었다 (CPSI 설문지 총점. 감염 지속군; 15.148 ± 6.798/ 완치군; 5.357 ± 7.025, p=0.001). 3. 증례-대조군 연구에서 마이코플라스마에 감염된 남성은 배뇨 시 (1c) 및 음경 끝 (2a) 통증이 일반적인 만성 전립선염이나 골반통 환자보다 더 많이 관찰되었다 (1c, 2a 모두 p=0.0001). 결론 M. genitalium 감염은 만성골반 통증 혹은 만성전립선염과 연관되어 있으며 이러한 골반 통증은 적절한 항생제 치료로 개선되었다. M. genitalium 감염에 의한 통증은 음경 첨부 통증이나 배뇨통이 특징적으로 관찰되어 드러나지 않는 요도염이 골반통 발생에 중요한 역할을 하고 있음을 추정케 한다. 주제어. 마이코플라즈마 제니탈리움, 골반통증, 전립선염
The purpose of this study was to investigate the irony comprehension in the adolescents with mild intellectual disability and to provide basic data for linguistic intervention and educational materials by investigating their ability to comprehend irony and the types of errors in the process. The subjects 40 children whose linguistic age is 8-10. Among them, chronological age of 20 mild intellectual disability adolescent (experimental group, EG) was 19-22 and of 20 general children (control group, CG) was identical to their linguistic age. For the purpose, a questionnaire consisting of a total of 20 items with five questions for each of the four functions (emphasis, reprimand/admonishment, sarcasm, and suggestion/agreement) of irony was used. The results are as follows: First, for the total score of irony comprehension, the mean score was found to be higher in CG compared to EG. Second, for the score of irony comprehension of each function, there were significant differences between two groups in all functions of emphasis, reprimand/admonishment, sarcasm, and suggestion/agreement. Third, for the score of type of irony comprehension, both group showed more errors in literal interpretation than in context-unrelated interpretation. This study examined the ability to comprehend irony in a group of adolescents with mild intellectual disability and a group of general children with identical chronological and linguistic ages and is expected to contribute to the development of intervention to improve their social relationship through communication enhancement. 본 연구에서는 경도지적장애 청소년을 대상으로 아이러니 이해 능력과 그에 따른 오류 유형을 알아보고 분석하여 아이러니 이해 양상에 대해 알아보고 언어적 중재 및 교육자료로 제공하고자 한다. 연구대상은 생활연령이 만 19~22세이면서 언어연령이 8~10세에 해당하는 경도지적장애 청소년 20명과 언어연령을 일치시킨 일반 아동 20명으로 총 40명을 대상으로 선정하였다. 연구방법은 4가지 기능(강조, 질책·훈계, 비꼼·빈정거림, 제안·동의)의 아이러니에서 5문항씩 총 20문항으로 제작하여 진행하였다. 연구 결과, 첫째, 아이러니 이해 전체 점수를 비교하였을 때, 경도지적장애 청소년 집단에 비해 언어연령 일치 일반아동 집단의 평균 점수가 더 높은 것으로 나타났다. 둘째, 경도지적장애 청소년과 언어연령 일치 일반아동의 기능별 아이러니 이해 점수를 살펴보았을 때 강조, 질책·훈계, 비꼼·빈정거림, 제안·동의 모두에서 유의한 차이를 나타내었다. 셋째, 경도지적장애 청소년과 언어연령 일치 일반아동의 아이러니 오류 유형을 살펴보았을 때, 두 집단 모두 문자적 해석이 문맥과 관련 없는 해석보다 많은 빈도의 오류를 보이는 것으로 나타났다. 본 연구는 경도지적장애 청소년 집단과 언어연령 일치 일반아동 집단을 대상으로 아이러니 이해 능력을 살펴보고 이에 대한 적절한 중재를 통해 경도지적장애 청소년의 의사소통을 증진시켜 이를 통해 적절한 사회적 관계를 맺고 유지할 수 있기를 기대한다.
Role of PKCζ signal pathway in the ovulatory process in the rat
서유미 Chonnam National Univ. 2007 국내박사
LH binding to its receptor can activate several pathways, including protein kinase A/C, phospholipase A2/C, and tyrosine kinase. cAMP-PKA or PKC pathway is considered to be the most important signals in LH action. However, previously studies shown that LH treatment of granulosa cells resulted in a rapid translocation of atypical PKCζ from cytoplasm to the plasma membrane and the stimulation of atypical PKCζ activity by immunocytochemical and kinase assay. Although the presence of PKCζ in the ovary has been reported (Cutler et al., 1993), the activation of a specific PKC isoform PKCζ by LH in granulosa cells of preovulatory follicles has not been previously documented. Therefore, to understand mechanism of PKCζ during ovulatory process, the present study was designed to identify PKCζ regulated-genes in rat ovarian preovulatory granulosa cells. Preovulatory granulosa cells, obtained from ovaries of PMSG-primed immature rats, were cultured in serum-free medium in the presence of myristoylated PKCζ pseudosubstrate peptide (myr-SIYRRGARRWRKL-NH2) and a similarly sized control peptide (myr-ITRARRAPSVAN-NH2) with or without LH for 1.5 h. Total RNA extracted from cultured granulosa cells was used for ACP RT-PCR. As a result, sixteen genes including testin and aminolevulinic acid synthase1 have been identified. Particularly, six genes (testin, glypican 4, retrovirus SC1, connective growth factor (ctgf), aminolevulinic acid synthase 1 (alas1), serum-inducible kinase(snk)) were rapidly stimulated by hCG. To determine mRNA expression and localization of these six genes, Northern blot analysis and in situ analysis was performed. Testin mRNA is rapidly and transiently stimulated in PMSG-primed rats with hCG, reaching a peak within 3 h. In situ hybridization analysis revealed that hCG treatment induced the expression of testin in granulosa cells of preovulatory follicles. Glypican-4 mRNA expression was increased from 3 h to 24 h after gonadotropin treatment in granulosa cells of preovulatory follicles. Increase in levels of ovarian retrovirus SC1 mRNA was detected at 3 and 6 h after hCG administration, and the levels rapidly declined. Furthermore, high expression of SC1 mRNA was localized to both granulosa cells of preovulatory follicles and the theca-interstitial layer at 3 h after hCG. Moderate levels of alas1 mRNA were detected in both immature untreated or PMSG-treated ovaries before hCG injection, and also rapidly and transiently stimulated alas1 mRNA expression in granulosa cells of growing follicles and preovulatory follicles, reaching a maximum stimulation 1 h after hCG treatment. Treatment of PMSG-primed rats with hCG for 1 h resulted in the rapid and transient stimulation of snk mRNA expression, reaching a maximum stimulation for 3 h after hCG treatment in granulosa cells of preovulatory follicles. The levels of snk mRNA declined by 6 h after treatment. Also, the rest of the ten genes were slowly stimulated by hCG. Northern analysis revealed that these ten gene expression was increased within 12-72 h after hCG treatment. As the next step, I examined a role of PKCζ for LH action during ovulation in cultured rat granulosa cells of preovulatory follicles. Sixteen genes regulated by PKCζ and PKCζ signaling pathway were mostly involved in cell survival, apoptosis, and differentiation in other system. To determine whether PKCζ signaling pathway by LH in preovulatory granulosa cells plays a role of inhibition of granulosa cell apoptosis, MTT assay and DNA fragmentation assay was performed on preovulatory granulosa cells cultured under serum-free conditions for 48 h with increasing dose of RO 31-8220 (RO), myristoylated PKCζ pseudosubstrate peptide or a similarly sized control peptide with or without LH. MTT assay was shown that apoptosis of preovulatory granulosa cells is stimulated by myristoylated PKCζ pseudosubstrate peptide and 10 μM RO known to be a potent inhibitor of PKC ζ. DNA fragmentation assay showd that 10 μM RO markedly increased the ladder-pattern fragmentation of genomic DNA of cells, which was known as a characteristic feature of apoptosis suggest granulosa cell survival role for PKCζ signaling pathway in ovulation. I then further investigated a part of the genes (testin, alas1) regulated following PKCζ activation during LH surge. Testin is widly expressed in normal tissues and is predicted to encode a highly conserved protein of 421 amino acids containing three C-terminal LIM domains. To access a role of testin during LH-induced ovulation, I examined hormonal expression of testin in the ovary. Treatment of PMSG-primed rats with hCG resulted in the rapid and transient stimulation of testin mRNA expression, reaching a maximum stimulation 3 h after treatment. Testin mRNA was undetected in the ovaries of 26-day-old immature rats. In the ovaries obtained 48 h after PMSG treatment, testin signals were detected in atretic follicles. Interestingly, in the ovaries of PMSG-primed immature rats followed by hCG stimulation for 3 h, testin mRNA was induced in granulosa cells of mature preovulatory follicles, but not of growing follicles, as well as atretic follicles. These observation raises the possibility that testin may play a role in granulosa cell apoptosis. I have also cloned rat testin cDNA. This cDNA clone of 1397 bp is a partial representation of 2.8 kb mRNA, containing a long open reading frame (ORF) of 1260 bp. The ORF in the testin cDNA predicts a 420 amino acid polypeptide. Comparison of the amino acid sequences of human and rat testin revealed that they display 86% overall identity. The rat testin protein sequence, like the murine sequence, contains three LIM domains. Collectively, hormonal regulation of testin suggests that testin may play a role as a cell survival and apoptotic factor leading granulosa cells of ovulating follicles and small follicles to survive or death during ovulatory process. Alas1 is perhaps best known for its role in catalyzing the condensation of glycine and succinyl-CoA to yield 5-aminolevulinate, a universal precursor of tetrapyrrole compounds that function in a variety of reactions including the biosynthesis of heme, the transport of single electrons, and the catalysis of redox reactions. Alas1 mRNA were detected in both immature untreated or PMSG-treated theca-interstitial cells of ovaries before hCG injection, and treatment of PMSG-primed rats with hCG, however, resulted in the rapid and transient stimulation of alas1 mRNA expression, reaching a maximum stimulation 1 h after hCG treatment in theca-interstitial cells as well as granulosa cells of preovulatory follicles and some growing follicles. In addition, alas1 mRNA signals were detected in corpus luteum of ovaries stimulated with hCG for 24 h. Next, I studied a role of alas1 using 5-aminolevulinate (ALA) or hemin, inhibitor of alas1. hCG/LH-stimulated alas1 mRNA was remarkably decreased by ALA or hemin in vivo and in vitro. Because induction of alas1 induce the synthesis of several members of the CYP family, I determined whether P450scc and aromatase are regulated by alas1. Furthermore, inhibition of alas1 mRNA by ALA or hemin decreased P450scc and aromatase mRNA expression in gonadotropin-stimulated ovary and preovulatory granulosa cells. Furthermore, alas1 inhibiton by ALA or hemin had not effect on ovulation suggesting a role for steroidogenesis during ovulation. In addition, I examined a signal pathway for LH action on testin and alas1 gene expression in cultured rat granulosa cells of preovulatory follicles. Treatment with an inhibitor of protein kinase A (PKA) did not affect LH-induced testin and alas1 levels. Interestingly, LH-induced testin and alas1 expression was suppressed by high dose of protein kinase C (PKC) inhibitor RO 31-8220 (10 μM), but not by low doses (0.1-1.0 μM), implicating the involvement of atypical PKCs. Treatment with the cell-permeable PKCζ-specific inhibitor pseudosubstrate peptide inhibited LH-induced alas1 expression, indicating the essential role of PKCζ. In summary, the expression of PKCζ-regulated genes, testin, and alas1 is induced by LH surge and they play critical roles during LH-induced ovulation.
급성 방광염에서의 항생제 처방현황 : 건강보험데이터베이스를 대항으로 한 데이터마이닝 연구
연구배경 : 단순방광염은 임상에서 흔히 접하게 되는 요로감염의 하나로 지정된 기간 동안의 건강보험심사평가원 청구 데이터베이스를 사용하여 우리나라에서 급성방광염 진단으로 항생제를 사용하는 양상을 분석하고자 한다. 대상 및 방법 : 2011년 1월 1일부터 2011년 12월 31일 까지 심사평가원에 보험 청구된 자료를 대상으로 하여 명세서(table-20)에서 청구 주진단명이 급성 방광염인 환자 71.052을 추출하였고, 이의 명세서 연결코드를 이용하여 원외처방내역(table-53)과 병합하였다. 결과 : 급성방광염 환자에서 56.5% (40,112명/71,052명)가 다양한 종류의 항생제를 처방 받았다. 항생제를 처방한 40,112명 중에서 2종 이상의 항생제를 사용한 군은 16.4% (6,599명/40,112명)인데 비해, 항생제 단독 요법을 사용한 경우는 83.6% (33,513명/40,112명)였다. 이 중 가장 대중적으로 사용되는 처방은 aminoglycoside계 약물의 1회 단독 주사 요법으로 확인되었다. 흥미롭게도, 우리나라에서는 몇몇 종류의 질정 형태의 항생제도 급성방광염 환자에서 빈번한 횟수로 처방되었다. 경구항생제에서 trimexoprime/sulfamethoxalzole (Bactrim), fosfomycin trometamol, nitrofurantoin, 그리고 pivmecillinam의 처방건수는 매우 낮았고, 그 외로 ciprofloxacin, levofloxacin, ofloxacin과 lomefloxacin등 의 fluoroquinolone계 약물과 cefaclor와 cefpodoxime proxetil같은 cephalosporin계 약물의 경구처방도 높은 비중을 차지하지 않았다. 결론 : 우리나라에서 급성방광염 환자들에게 사용되는 약물치료는 원칙 없이 다양하게 사용되고 있다. 이러한 항생제치료는 과학적 근거와 요로감염의 임상진료지침에 맞춰서 시행되어야 한다. Objectives: We analyzed one the antibiotics prescribing pattern from the patients of acute cystitis in Korea using data-mining technique from Health Insurance Review & Assessment Service (HIRA) Claims Database Methods and Materials: We obtained information from the HIRA Claims Database, 2011. At first, we selected the patient’s records of acute cystitis (N300) from the Table -20. We can get 71, 052 acute cystitis records. We used key codes from 71, 052 cases for attaching the data from Table-20 to prescribing data from Table-53. Results: 56.5 % of patients (40,112/71,052) were treated with various types of antibiotics. A single antibiotic was prescribed to 83.6% (33,513/40,112) of the patients, while more than two antibiotics were prescribed into 16.4% (6,599/40, 112) of cystitis patients. The most popular treatment pattern is injection of various kinds of aminoglycoside. Interestingly, some types of intra-vaginal suppositories were very popular prescribing patterns for acute cystitis in Korea. The oral antibiotics, such as trimexoprime-sulfamethoxalzole (bactrim), fosfomycin trometamol, nitrofurantoin or its derivatives, and pivmecillinam were prescribed very little. The portion of various fluoroquinolone prescriptions, such as ciprofloxacin, levofloxacin, ofloxacin, and lomefloxacin, and the ration of oral cephalosporin prescriptions, such as cefaclor, and cefpodoxime proxetil were not high. Conclusions: These findings demonstrated that the antibiotic prescription pattern for the patients of acute cystitis in Korea was twisted. We should use antibiotics with the scientific evidence and clinical guidelines of urinary tract infections.
현장학습을 통한 유아의 교통안전교육이 교통안전지식 및 문제해결사고에 미치는 영향
서유미 숭실대학교 교육대학원 2008 국내석사
현대사회의 눈부신 업적 중 하나가 교통수단의 발달일 것이다. 그러나 교통수단의 발달과 함께 교통사고의 발생율도 매년 증가되고 있다. 1~9세 어린이의 사망원인이 1위가 교통사고임을 감안해 볼 때 유아들에게 교육을 통한 교통안전에 대한 안전의식을 갖게 하는 것은 반드시 필요하다. 그러므로 본 연구는 현장학습을 적용한 교통안전교육이 유아의 교통안전지식과 교통안전 문제해결사고에 미치는 영향을 알아보고자 하는데 목적이 있다. 본 연구의 연구문제는 다음과 같다. 첫째, 현장학습을 통한 교통안전교육이 유아의 교통안전지식에 미치는 영향 은 어떠한가? 둘째, 현장학습을 통한 교통안전교육이 유아의 교통안전 문제해결사고에 미치는 영향은 어떠한가? 연구의 대상은 만 5세 유아 36명으로 연구의 목적에 따라 실험집단과 통제집단으로 18명씩 무선배치 하였다. 본 연구는 4주(12회)의 연구기간 동안 교통안전 프로그램이 교통안전교육, 현장학습, 사후활동으로 실시되는데 실험집단은 교통안전교육, 현장학습, 사후활동이 모두 실시되었고 통제집단은 교통안전교육만 실시되었다. 검사도구인 교통안전지식은 박유영(2004)의 검사도구를 본 연구에 적합하게 선정?조정한 후 신뢰도를 확인 후 사용하였고 교통안전 문제해결사고는 박유영(2004)의 검사도구를 그대로 사용하였다. 사전검사를 거쳐 두 집단간의 동질성을 확인한 후 실험처치 하였다. 실험은 9월 27일부터 10월 19일까지 진행되었고, 실험처치를 마치고 1주후에 사전검사와 동일한 방법으로 사후검사를 실시하였다. 결과처리는 사후검사를 이용하여 t-test로 검증하였다. 본 연구의 주요 연구결과는 다음과 같다. 첫째, 현장학습을 통한 교통안전교육이 유아의 교통안전지식을 높이는데 효과가 있었다. 실험집단과 통제집단의 사후검사 비교에 있어 실험집단의 점수가 높게 나와 교통안전지식에 효과가 있음이 확인되었다. 둘째, 현장학습을 통한 교통안전교육이 유아의 교통안전 문제해결사고를 높이는데 효과가 있었다. 실험집단과 통제집단의 사후검사 비교에 있어 실험집단의 점수가 높게 나와 교통안전 문제해결사고 능력에도 효과가 있음이 확인되었다. 본 연구의 결과로 볼 때 현장학습을 통한 교통안전교육은 유아의 교통안전지식과 교통안전 문제해결사고에 큰 효과가 있음을 알 수 있었다. 앞으로 체계적인 현장학습의 교통안전교육 프로그램을 개발하여 유아교육현장에서 활성화 하여야 할 것이다. One of today's most imminent advancement is in the field of transportation. However, the rate of traffic accidents has been also increasing every year alongside. Since traffic accident was the number one cause of death among children with age range from 1 to 9, it is imperative to guide small children to have affirmative idea about traffic safety through means of education. The purpose of this study is to see how traffic safety education program with field experience course affects young children's knowledge and responsiveness on traffic safety. The key problem of this study is as follows. First, how does traffic safety education program with field experience course affect young children's knowledge on traffic safety? Second, how does traffic safety education program with field experience course affect young children's responsiveness on traffic safety? The study subjects were 36 children, all five years old. They were randomly divided into two groups, experiment and control. The study was performed over the period of four weeks - 12 sessions - and traffic safety program given consisted of lectures on traffic safety, field experience course, and post activities. The experiment group was given all three programs, traffic safety lectures, field experience course, and post activities. On the other hand, only traffic safety lectures were given to the control group. The inspection method for traffic safety knowledge was extracted and modified to fit this study from Park You Young's paper(2004). The reliability for the modified method was checked before hand. and the responsiveness on traffic safety were inspected with the method from the same paper, unmodified. A preliminary test was done to ensure homogeneity between the two groups before the experiment began. The experiment was performed from September 27th through October 19th. A post test was given one week after the experiment was over and was identical to the previous preliminary test. The post test result was processed with T-test method. The conclusion from this study is as follows. First, traffic safety education program with field experience course had evident effect on small children's knowledge on the matter, confirmed by the fact that the experiment group demonstrated higher scores from post test compare to the control group. Second, the same program had evident effect on small children's responsiveness on traffic safety, confirmed by the fact that the experiment group had higher scores than the other group. As seen from the result of this study, field experience course has evident effect on small children's knowledge and responsiveness on traffic safety. Systematic field study programs for traffic safety course are expected to be developed and should be actively implemented into small children's education program.