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Lee, Byoungsoo,Yeon, Kyung-Min,Shim, Jongmin,Kim, Sang-Ryoung,Lee, Chung-Hak,Lee, Jinwoo,Kim, Jungbae American Chemical Society 2014 Biomacromolecules Vol.15 No.4
<P>Highly effective antifouling was achieved by immobilizing and stabilizing an acylase, disrupting bacterial cell-to-cell communication, in the form of cross-linked enzymes in magnetically separable mesoporous silica. This so-called “quorum-quenching” acylase (AC) was adsorbed into spherical mesoporous silica (S-MPS) with magnetic nanoparticles (Mag-S-MPS), and further cross-linked for the preparation of nanoscale enzyme reactors of AC in Mag-S-MPS (NER-AC/Mag-S-MPS). NER-AC effectively stabilized the AC activity under rigorous shaking at 200 rpm for 1 month, while free and adsorbed AC lost more than 90% of their initial activities in the same condition within 1 and 10 days, respectively. When applied to the membrane filtration for advanced water treatment, NER-AC efficiently alleviated the biofilm maturation of <I>Pseudomonas aeruginosa</I> PAO1 on the membrane surface, thereby enhancing the filtration performance by preventing membrane fouling. Highly stable and magnetically separable NER-AC, as an effective and sustainable antifouling material, has a great potential to be used in the membrane filtration for water reclamation.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bomaf6/2014/bomaf6.2014.15.issue-4/bm401595q/production/images/medium/bm-2013-01595q_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bm401595q'>ACS Electronic Supporting Info</A></P>
지역 문화인물 콘텐츠개발연구 -보령, 토정(土亭) 이지함(李之?) 을 중심으로-
이해광 ( Haekwang Lee ),김병수 ( Byoungsoo Kim ) 한국영상미디어협회 2012 예술과 미디어 Vol.11 No.1
본 논문은 충남 보령의 역사인물인 토정 이지함과 그의 저술로 추정되는 <토정비결>의 문화콘텐츠 개발에 관한 제안을 연구한 것이다. 문화콘텐츠에 대한 관심과 수요가 증대하고 실제 다양한 문화콘 텐츠들이 제작, 유통됨에도 우리나라의 지역 문화콘 텐츠는 단순한 관광상품 수준을 벗어나지 못하고 있다. 이에 운명학의 대가 이지함을 중심으로 다양 한콘텐츠 개발 방법을 구체적인 사례를 통해 제시 하고 한다. This paper is a study on the means for Tojeong Lee Jiham, a historical figure of Boryeong, Chungnam Province, and his work <Tojeong Bigyeol> to be developed as traditional culture contents. Interest and needs on traditional culture contents are enlarging, and so numerous traditional culture contents are produced and on the market nowadays. However, traditional culture contents in Korea`s rural areas are utilized no more than as simple tourist courses. In this regard, this study exemplifies the methods of developing various contents, concentrating on Lee Jiham, a master in Korean divination.
Lee, Jinwoo,Na, Hyon Bin,Kim, Byoung Chan,Lee, Jin Hyung,Lee, Byoungsoo,Kwak, Ja Hun,Hwang, Yosun,Park, Je-Geun,Gu, Man Bock,Kim, Jaeyun,Joo, Jin,Shin, Chae-Ho,Grate, Jay W.,Hyeon, Taeghwan,Kim, Jungb Royal Society of Chemistry 2009 Journal of materials chemistry Vol.19 No.42
<P>A magnetically-separable and highly-stable enzyme system was developed by adsorption of enzymes in superparamagnetic hierarchically ordered mesocellular mesoporous silica (M-HMMS) and subsequent enzyme crosslinking. Superparamagnetic nanoparticles were homogeneously incorporated into hierarchically-ordered mesocellular mesoporous silica (HMMS) by the decomposition of a preformed iron propionate complex. The size of the incorporated superparamagnetic nanoparticles was around 5 nm, generating a magnetically separable host with high pore volumes and large pores (M-HMMS). α-chymotrypsin (CT) was adsorbed into M-HMMS with high loading (∼30 wt%) in less than 30 minutes. Glutaraldehyde (GA) treatment of adsorbed CT resulted in nanometer scale crosslinked enzyme aggregates in M-HMMS (CLEA-M). The activity of these CT aggregates in M-HMMS (CLEA-M-CT) was 34 times than that of simply adsorbed CT in M-HMMS, due to an effective prevention of enzyme leaching during washing <I>via</I> a ship-in-a-bottle approach. CLEA-M-CT maintained the initial activity not only under shaking (250 rpm) for 30 days, but also under recycled uses of 35 times. The same approach was employed for the synthesis of CLEA-M of lipase (CLEA-M-LP), and proven to be effective in improving the loading, activity, and stability of enzyme when compared to those of adsorbed LP in M-HMMS.</P> <P>Graphic Abstract</P><P>Crosslinked enzyme aggregates in magnetitie-coated mesoporous silica, fabricated <I>via</I> a two-step procedure of enzyme adsorption and crosslinking, were highly active, highly stable, and easily recyclable using a magnet. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b909109b'> </P>
이병수(Byoungsoo Lee),박민규(Min-Kyu Park),성금길(Kum-Gil Sung) 제어로봇시스템학회 2010 제어·로봇·시스템학회 논문지 Vol.16 No.6
SD (Smart Door) is a human friendly power-assisted door system initially targeted for passenger car doors. The Smart Door offers comfort and safety to passengers or/and drivers by supplying additional power. Amount of power supplied by the Smart Door system is depend on the environment where the automotive is situated. It realizes comfort, for example, when the force applied by the passenger to the door is expected to be abnormal, the SD system tries to compensate passenger"s effort by supplying additional force. In this study, to enhance the ease of opening and closing the doors of the passenger vehicle, a Smart Door with a power assist mechanism consisting of a motor was developed and analysed. A power assist mechanism mounted within the vehicle’s door is designed and modeled for simulation purpose. The required force necessary to control the designed mechanism during the vehicle’s roll, pitch and the opening angle of the door has been considered. To this end, we propose a power-assisting control strategy called "gravity cancellation". The system is analysed by numerical simulation with the gravity cancellation control algorithm.