Effective Antifouling Using Quorum-Quenching Acylase Stabilized in Magnetically-Separable Mesoporous Silica

다국어 초록 (Multilingual Abstract)

<P>Highly effective antifouling was achieved by immobilizing and stabilizing an acylase, disrupting bacterial cell-to-cell communication, in the form of cross-linked enzymes in magnetically separable mesoporous silica. This so-called “quorum-quenching” acylase (AC) was adsorbed into spherical mesoporous silica (S-MPS) with magnetic nanoparticles (Mag-S-MPS), and further cross-linked for the preparation of nanoscale enzyme reactors of AC in Mag-S-MPS (NER-AC/Mag-S-MPS). NER-AC effectively stabilized the AC activity under rigorous shaking at 200 rpm for 1 month, while free and adsorbed AC lost more than 90% of their initial activities in the same condition within 1 and 10 days, respectively. When applied to the membrane filtration for advanced water treatment, NER-AC efficiently alleviated the biofilm maturation of <I>Pseudomonas aeruginosa</I> PAO1 on the membrane surface, thereby enhancing the filtration performance by preventing membrane fouling. Highly stable and magnetically separable NER-AC, as an effective and sustainable antifouling material, has a great potential to be used in the membrane filtration for water reclamation.</P><P><B>Graphic Abstract</B>
<IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bomaf6/2014/bomaf6.2014.15.issue-4/bm401595q/production/images/medium/bm-2013-01595q_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bm401595q'>ACS Electronic Supporting Info</A></P>