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TANNylated-AAV for Enhanced Gene Delivery
Zheng Rong LAU,Ginah HAN,Jae-Hyung JANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4
Adeno-associated virus (AAV) has received tremendous attention for developments in gene therapy applications, leading to several approvals of AAV-based gene therapy products such as Glybera, Zolgensma, and Luxturna. However, much can still be done in optimizing the efficacy of AAV as a delivery vehicle for gene therapy. Among the many drawbacks of AAV as a delivery vector, the problem of its relatively low genome packaging capacity of only about 4.7 kb is especially detrimental, resulting in cases of which transgene need to be fragmented and packaged separately prior to administration. Herein, we demonstrate that through rather simple tannic acid treatment onto AAV to form tannic acid-treated AAV (namely TANNylated-AAV), the transduction efficiency is enhanced. Tannic acid is found to have high affinity to proline-rich extracellular matrices (ECM) and collagens, which leads us to hypothesize that TANNylated-AAV can allow for enhanced targeted delivery. We utilized hypoxic ischemic (HIE) brain injury mouse model to observe enhanced AAV transduction towards ECM-rich reactive astrocytes populated glial scar region of infarct area.
Polydopamine Coated Multi-Layered PCL Fiber as a Drug Delivery Carrier
오석민,김유진,이희형,한지나,( Lau Zheng Rong ),장재형 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
The spontaneous formation of multi-layers by an electrospinning technique is an unconventional result considering that conventional electrospinning generates a non-discrete, continuous fibrous structure. Dopamine was used as a salt during the fabrication by electrospinning to fabricate multi-layered PCL fiber. The salts are conventionally removed from the fabricated fiber after fabrication. In this study, we have studied the uniform coating of fiber surface by polymerizing dopamine released from the multi-layered PCL fiber. the multi-layered PCL fiber uniformly coated by polydopamine was characterized by SEM, drug release profile in different pH conditions. Also, Adeno-associated virus (AAV) can be attached to the surface of the coating to enable sequential delivery of surface AAV and fiber internal drug. This multi-layered PCL fiber uniformly coated by polydopamine will contribute significant advancements into drug release control and gene therapy.