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Zhao, P. Y.,Zhang, Z. F.,Lan, R. X.,Liu, W. C.,Kim, I. H. Cambridge University Press 2017 Animal Vol.11 No.6
<P>It is well known that energy plays an important role in sow growth and development. Increasing the utilization of lipids will be beneficial to sows. Emulsifiers are substances which stabilize mixtures and prevent oil and water from separating, thereby enhancing the digestion of lipids. This study was conducted to evaluate the effect of dietary emulsifier (lysophospholipids (LPL)) supplementation in diets differing in fat contents on growth performance, nutrient digestibility and milk composition in lactating sows, as well as performance and fecal score in piglets. A total of 32 multiparous sows (Landrace×Yorkshire) were used in a 21-day experiment. On day 110 of gestation, sows were weighed and moved into the farrowing facility, randomly assigned in a 2×2 factorial arrangement according to their BW with two levels of LPL (0 and 30 mg/kg) and two levels of fat (4.75% and 2.38% fat; 13.66 and 13.24 MJ/kg). BW loss and backfat thickness loss were decreased (<I>P</I><0.05) by LPL supplementation. Backfat thickness at weaning was higher (<I>P</I><0.05) in sows fed LPL supplementation diets. The apparent total tract digestibility of dry matter, nitrogen, gross energy and crude fat in sows fed LPL diets was increased (<I>P</I><0.05) compared with those fed non-LPL diets. Sows fed the high-fat diets had higher (<I>P</I><0.05) milk fat on day 10 and milk lactose on day 20 than those fed the low-fat diets. Milk fat and lactose concentrations in LPL supplementation treatments was increased (<I>P</I><0.05) compared with non-LPL treatments on day 10 and day 20, respectively. Positive interaction effects (<I>P</I><0.05) between fat and LPL were observed for milk fat concentration on day 10. In conclusion, LPL addition decreased BW loss and backfat thickness loss, improved nutrient digestibility and milk fat as well as milk lactose concentrations. In addition, there was a complementary positive effect of dietary fat and LPL supplementation on milk fat concentration in lactating sows.</P>
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Diagnostic Accuracy of 18F-FDG-PET in Patients with Testicular Cancer: a Meta-analysis
Zhao, Jing-Yi,Ma, Xue-Lei,Li, Yan-Yan,Zhang, Bing-Lan,Li, Min-Min,Ma, Xue-Lei,Liu, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8
Objective: Fluorine-18-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is a new technique for identifying different malignant tumors using different uptake values between tumor cells and normal tissues. Here we assessed the diagnostic accuracy of 18F-FDG-PET in patients with testicular cancer by pooling data of existing trials in a meta-analysis. Methods: PubMed/MEDLINE, Embase and Cochrane Central Trials databases were searched and studies published in English relating to the diagnostic value of FDG-PET for testicular cancer were collected. The summary receiver operating characteristic (SROC) curve was used to examine the FDG-PET accuracy. Results: A total of 16 studies which included 957 examinations in 807 patients (median age, 31.1 years) were analyzed. A meta-analysis was performed to combine the sensitivity and specificity and their 95% confidence intervals (CIs), from diagnostic odds ratio (DOR), positive likelihood ratios (PLR), negative likelihood ratio (NLR). SROC were derived to demonstrate the diagnostic accuracy of FDG-PET for testicular cancer. The pooled sensitivity and specificity were 0.75 (95% confidence interval (CI), 0.70-0.80) and 0.87 (95% CI, 0.84-0.89), respectively. The pooled DOR was 35.6 (95% CI, 12.9-98.3). The area under the curve (AUC) was 0.88. The pooled PLR and pooled NLR were 7.80 (95% CI, 3.73-16.3) and 0.31 (95% CI, 0.23-0.43), respectively. Conclusion: In patients with testicular cancer, 18F-FDG-PET demonstrated a high SROC area, and could be a potentially useful tool if combined with other imaging methods such as MRI and CT. Nevertheless, the literature focusing on the use of 18F-FDG-PET in this setting still remains limited.
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Haochi Liu,Lan Ding,Ligang Chen,Yanhua Chen,Tianyu Zhou,Huiyu Li,Yuan Xu,Li Zhao,Ning Huang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.69 No.-
Biomass carbon dots (CDs) prepared by sweet potato peels were superior fluorophores with low toxicity and excellent photostability. A novel designed fluorescence probe for specific recognition and sensitive detection of oxytetracycline (OTC) was fabricated with CDs and molecularly imprinted polymer (MIP). The quenching of CDs happened when rebinding with OTC due to electron-transfer-induced fluorescence quenching mechanism. The fluorescence probe was successfully applied in honey with the recoveries ranging from 90.2% to 97.3%. The detection limit of OTC was 15.3 ng mL−1. This work provides promising perspectives that the development of fluorescent MIP will be valuable for rapid analysis in complex samples.
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Fatigue life prediction and reliability analysis of the forage crusher rotor
Haixu Zhao,Zhiping Zhai,Yingsheng Mou,Le Liu,Yuezheng Lan,Hongmei Cui 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.4
To accurately predict fatigue life and reliability of the forage crusher rotor, the stress load spectrum of the rotor is obtained via the two-way fluid-structure coupling method, which calculates the coupled flow field in the forage crusher and rotor structure. The fatigue life of the rotor is predicted, and its reliability is analyzed. Finally, the rotor is optimized by a multiisland genetic algorithm. The results indicate that the fatigue life prediction based on fracture mechanics theory and the Gerber average stress correction method is more suitable for the prediction of the rotor’s fatigue life. After optimization, the fatigue life cycles of the rotor are increased from 1.229×10 8 to 7.67×10 8 and the reliability is increased from 89.4 % to 99.7 %. This meets the reliability requirement of key parts of agricultural and animal husbandry machinery. This study provides a reference for the prediction and optimization of fatigue life and reliability of forage crushers.
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Applications of Heavy Ion Irradiation in Photonic Crystal Research
Ziqiang Zhao,Yingmin Liu,Yan Chen,Ding Lan,Yuren Wang 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.55 No.6
Photonic crystals (PC) have received extensive attention for the photonic band gap (PBG). The polystyrene (PS) particles bottom-up approach is a productive method for photonic crystal manufacture, this kind of photonic crystals having an unique PBG that depends on the particle’s shape, sizes and defects. Heavy ion irradiation is a very useful method to induce defects in PC and change the shapes of the particles to tune the PBG. MeV heavy ion irradiation leads to an anisotropic deformation of the particles from spherical to ellipsoidal, the aspect ratio of which can be precisely controlled by using the ion energy and flux. Sub-micrometer PS particles were deposited on a Cu substrate and were irradiated at 230 K by using heavy ion energy and fluence in the range from 2 to 10 MeV and 1 × 1014 cm−2 to 1 × 1015 cm−2, respectively. Photonic crystals (PC) have received extensive attention for the photonic band gap (PBG). The polystyrene (PS) particles bottom-up approach is a productive method for photonic crystal manufacture, this kind of photonic crystals having an unique PBG that depends on the particle’s shape, sizes and defects. Heavy ion irradiation is a very useful method to induce defects in PC and change the shapes of the particles to tune the PBG. MeV heavy ion irradiation leads to an anisotropic deformation of the particles from spherical to ellipsoidal, the aspect ratio of which can be precisely controlled by using the ion energy and flux. Sub-micrometer PS particles were deposited on a Cu substrate and were irradiated at 230 K by using heavy ion energy and fluence in the range from 2 to 10 MeV and 1 × 1014 cm−2 to 1 × 1015 cm−2, respectively.
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African Maternal Origin and Genetic Diversity of Chinese Domestic Donkeys
Lei, Chu-Zhao,Ge, Qing-Lan,Zhang, Hu-Cai,Liu, Ruo-Yu,Zhang, Wei,Jiang, Yong-Qing,Dang, Rui-Hua,Zheng, Hui-Ling,Hou, Wen-Tong,Chen, Hong Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5
The origin of domestic donkeys in China has been controversial. To clarify the origin of Chinese domestic donkeys, we investigated the partial mitochondrial D-loop sequences of 126 samples from 12 native breeds. The results revealed two mitochondrial origins, lineage Somali and lineage Nubian of African wild ass detected in Chinese domestic donkeys. Lineage Somali was predominant in Chinese domestic donkey breeds. The pattern of genetic variation in ass mtDNA D-loop sequences indicated that the two lineages Somali and Nubian from China had undergone population expansion events. In a combined analysis of lineages Somali and Nubian between previously published sequences from other countries/regions and sequences of Chinese domestic donkeys, the results indicated that the two lineages of Chinese domestic donkeys were from Africa and supported the African maternal origins of Chinese domestic donkeys. There was no obvious geographical structure in Chinese domestic donkey breeds, but the population showed abundant mtDNA diversity. The spread routes of Chinese domestic donkeys were also discussed.
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Lei, Chu-Zhao,Zhang, Wei,Chen, Hong,Lu, Fan,Ge, Qing-Lan,Liu, Ruo-Yu,Dang, Rui-Hua,Yao, Yun-Yi,Yao, Li-Bo,Lu, Zi-Fan,Zhao, Zhong-liang Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.4
Little is known about the origin and genetic diversity of swamp buffaloes in China. To obtain more knowledge on genetics of the water buffalo in China, the complete mitochondrial D-loop sequences of 30 samples from 6 native types were investigated. The results revealed 12 mitochondrial haplotypes with 50 polymorphic sites. Among these polymorphic sites, there were 49 transitions and 1 transversion. The average nucleotide diversity and haplotype diversity estimated from mtDNA D-loop region in 6 Chinese water buffalo types were 0.00684 and 0.798, respectively, showing rather abundant mitochondrial genetic diversity. The Neighbor-Joining (NJ) tree of mtDNA of Chinese water buffaloes was constructed according to the 12 haplotypes. The NJ tree indicated two lineages being designated lineage A and lineage B, in which lineage A was predominant, and lineage B was at low frequency. The new lineage B was first discovered and defined in 6 Chinese water buffalo types. These results showed that two different maternal lineages were involved in the origin of domestic swamp buffaloes in China and the lineage B was probably an introgression from Southeast Asian buffaloes.
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Structural and functional analyses of the lipase CinB from <i>Enterobacter asburiae</i>
Shang, Fei,Lan, Jing,Liu, Wei,Chen, Yuanyuan,Wang, Lulu,Zhao, Jing,Chen, Jinli,Gao, Peng,Ha, Nam-Chul,Quan, Chunshan,Nam, Ki Hyun,Xu, Yongbin Elsevier 2019 Biochemical and biophysical research communication Vol.519 No.2
<P><B>Abstract</B></P> <P>Lipases are widely present in various plants, animals and microorganisms, constituting a large category of enzymes. They have the ability to catalyze the cleavage of ester bonds. The lipase CinB from <I>Enterobacter asburiae</I> (<I>E. asburiae</I>) is an acetyl esterase. The primary amino acid sequence suggests that the EaCinB protein belongs to the α/β-hydrolase (ABH) superfamily of the esterase/lipase superfamily. However, its molecular functions have not yet been determined. Here, we report the crystal structure of <I>E. asburiae</I> CinB at a 1.45 Å resolution. EaCinB contains a signal peptide, cap domain and catalytic domain. The active site of EaCinB contains the catalytic triad (Ser180-His307-Asp277) on the catalytic domain. The oxyanion hole is composed of Gly106 and Gly107 within the conserved sequence motif HGGG (amino acid residues 106–109). The substrate is accessible between the α1 and α2 helices or the α1 helix and catalytic domain. Narrow substrate pockets are formed by the α2 helix of the cap domain. Site-directed mutagenesis showed that EaCinB-W208H exhibits a higher catalytic ability than EaCinB-WT by approximately nine times. Our results provide insight into the molecular function of EaCinB.</P> <P><B>Highlights</B></P> <P> <UL> <LI> EaCinB structure contains a signal peptide, cap domain and catalytic domain. </LI> <LI> The active site of EaCinB contains the catalytic triad (Ser180-His307-Asp277). </LI> <LI> The oxyanion hole is composed of Gly106 and Gly107. </LI> <LI> Substrate-binding mode of EaCinB are proposed. </LI> <LI> Site-directed mutagenesis showed that EaCinB-W208H exhibits a higher catalytic ability than EaCinB-WT. </LI> </UL> </P>
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