Cold-Stress Response of Probiotic Lactobacillus plantarum K25 by iTRAQ Proteomic Analysis

Shaoli Liu,Yimiao Ma,Yi Zheng,Wen Zhao,Xiao Zhao,Tianqi Luo,Jian Zhang,Zhen-Nai Yang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.2

To understand the molecular mechanism involved in the survivability of cold-tolerant lactic acid bacteria was of great significance in food processing, since these bacteria play a key role in a variety of low-temperature fermented foods. In this study, the cold-stress response of probiotic Lactobacillus plantarum K25 isolated from Tibetan kefir grains was analyzed by iTRAQ proteomic method. By comparing differentially expressed (DE) protein profiles of the strain incubated at 10oC and 37oC, 506 DE proteins were identified. The DE proteins involved in carbohydrate, amino acid and fatty acid biosynthesis and metabolism were significantly down-regulated, leading to a specific energy conservation survival mode. The DE proteins related to DNA repair, transcription and translation were up-regulated, implicating change of gene expression and more protein biosynthesis needed in response to cold stress. In addition, two-component system, quorum sensing and ABC (ATP-binding cassette) transporters also participated in cell cold-adaptation process. These findings provide novel insight into the cold-resistance mechanism in L. plantarum with potential application in low temperature fermented or preserved foods.

An Investigation on Stress States of the Cataclastic Rock Specimen under Confined Compression Based on Modified Thick-walled Cylinder Model

Jin Zhang,Ronggui Deng,Zhibin Zhong,Peipei Wu,Shaoli Qi 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.10

In high in-situ stress zones, cataclastic rocks have exerted a significant influence on the stability of engineering structures. However, the mechanical properties of cataclastic rocks can not be accurately studied due to difficulties in sampling and laboratory testing. In this paper, laterally confined compression tests for specimens that were obtained by the developed in-situ sampling devices were performed to investigate the stress states. A modified thick-walled cylinder model considering axial shear stress was introduced. The interaction mechanics model for a compressed sample and an equivalent single-layer cylinder was established. Based on the principle of elasticity, the laterally confined stress, the axial shear stress, and the axial stress of the sample were derived and obtained. Moreover, the effects of mechanical and geometric parameters of the equivalent cylinder on the force condition of the specimen were analyzed. The results show that the confined stress is positively correlated with the equivalent elastic modulus and the geometric factor. Therefore, the confined stress can be strengthened by increasing the elastic modulus, alternatively, increasing the outer diameter and decreasing the inner diameter. However, the axial stress is little affected by the equivalent elastic modulus and the geometric factor and is not affected by the equivalent Poisson's ratio. As the distance from the center height increases, the axial stress decreases linearly and the difference in axial stress becomes larger considering axial shear stress and ignoring axial shear stress.

Global Practical Tracking via Adaptive Event-triggered Output Feedback for Nonlinear Systems with Function Control Coefficients

Yu Zhang,Shaoli Jin,Hui Li 제어로봇시스템학회 2023 제어로봇시스템학회 국제학술대회 논문집 Vol.2023 No.10

Parameter design of active damping‑based shunt hybrid active power filters

Jianhang Lu,Miao Zhang,Junming Wen,Dali Zheng,Shaoli Deng,Xiang Shi,Ling Yang 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.5

This paper presents the parameter design of a new topology of hybrid active power filter (HAPF), which makes the system capacity smaller. Based on the main topological circuit, the passive LC single-tuned circuit shunted into the HAPF effectively reduces the capacity of the system. By introducing the grid voltage feedforward method, the DC-side voltage control becomes relatively stable, and the fundamental reactive current flowing through the converter is effectively reduced. Meanwhile, to suppress the resonance of the HAPF, the state variable feedback method is adopted. According to theoretical analysis, a simulation model of the system is built in the MATLAB environment. Then a low-voltage experimental HAPF platform is built to validate the result of the simulations.

KLF9 promotes autophagy and apoptosis in T-cell acute lymphoblastic leukemia cells by inhibiting AKT/mTOR signaling pathway

Zhao Jie,He Shaolong,Xiang Chenhuan,Zhang Shaoli,Chen Xinyue,Lu Xinyi,Yao Qiong,Yang Liping,Ma Liangming,Tian Weiwei 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.3

Background T -cell acute lymphoblastic leukemia (T-ALL) is considered a malignant tumor with a high mortality rate. To combat this disease, exploring the mechanism of T-ALL progression is urgently needed. Krüppel-like factors (KLFs) are known as the transcription factors and mediate series of biological processes. KLF9 is a member of the KLF family which could serve as a tumor suppressor gene in most solid tumors. GEO Database analysis showed that KLF9 expression in normal T cells was higher than T-ALL cell lines and patients. However, the possible role of KLF9 in T-ALL progression is still unclear. Objective To uncover the possible eff ects of Krüppel-like transcription factor 9 (KLF9) on the progression of T-Acute lymphoblastic leukemia (T-ALL). Results The expression of KLF9 was low in human T-ALL cells. KLF9 suppressed the viability of T-ALL cells. In addition, KLF9 stimulated the apoptosis as well as autophagy of T-ALL cells. Mechanically, KLF9 suppressed AKT/mTOR pathway in T-ALL cells. Conclusion KLF9 suppressed viability and promoted autophagy as well as apoptosis in T-ALL cells by inhibiting AKT/ mTOR pathway.

Infection of tomato by Tomato Yellow Leaf Curl Virus alters the foraging behavior and parasitism of the parasitoid Encarsia formosa on Bemisia tabaci

Xin Liu,Yanyan He,Wen Xie,Qingjun Wu,Youjun Zhang,Yong Liu,Shaoli Wang 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2

Encarsia formosa Gahan is a solitary endoparasitoid that is commercially reared and released for augmentative biological control of whiteflies including Bemisia tabaci (Gennadius). Bemisia tabaci biotypes B and Q are two most invasive species that greatly reduce crop yields in China by feeding on plant sap and by transmitting Tomato Yellow Leaf Curl Virus (TYLCV). The effects of TYLCV infection of tomato on E. formosa foraging on B. tabaci B and Q are unknown. In Y-tube olfactometer assays in the present study, E. formosa significantly preferred TYLCVinfected tomato plants over TYLCV-free plants. The wasp females also significantly preferred TYLCV-infected tomato plants infested with 3rd-instar nymphs of B. tabaci biotype Q over TYLCV-free plants with biotype Q nymphs. However, no significant differences were observed when B. tabaci biotype B was infested on tomato plants. The oviposition bioassays confirmed that TYLCV infection on tomato plants resulted in the recruitment of parasitoids. These results indicate that TYLCV-infection of tomato increase the foraging of E. formosa on B. tabaci, as differs on the B and Q biotypes.

Alternative Nanostructures for Thermophones

Aliev, Ali E.,Mayo, Nathanael K.,Jung de Andrade, Monica,Robles, Raquel O.,Fang, Shaoli,Baughman, Ray H.,Zhang, Mei,Chen, Yongsheng,Lee, Jae Ah,Kim, Seon Jeong American Chemical Society 2015 ACS NANO Vol.9 No.5

<P>Thermophones are highly promising for applications such as high-power SONAR arrays, flexible loudspeakers, and noise cancellation devices. So far, freestanding carbon nanotube aerogel sheets provide the most attractive performance as a thermoacoustic heat source. However, the limited accessibility of large-size freestanding carbon nanotube aerogel sheets and other even more exotic materials recently investigated hampers the field. We describe alternative materials for a thermoacoustic heat source with high-energy conversion efficiency, additional functionalities, environmentally friendly, and cost-effective production technologies. We discuss the thermoacoustic performance of alternative nanostructured materials and compare their spectral and power dependencies of sound pressure in air. We demonstrate that the heat capacity of aerogel-like nanostructures can be extracted by a thorough analysis of the sound pressure spectra. The study presented here focuses on engineering thermal gradients in the vicinity of nanostructures and subsequent heat dissipation processes from the interior of encapsulated thermoacoustic projectors. Applications of thermoacoustic projectors for high-power SONAR arrays, sound cancellation, and optimal thermal design, regarding enhanced energy conversion efficiency, are discussed.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2015/ancac3.2015.9.issue-5/nn507117a/production/images/medium/nn-2014-07117a_0014.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn507117a'>ACS Electronic Supporting Info</A></P>