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Lee Ji Yeon,Park Jeong-Yong,Jeong Yulah,Kang Chang-Ho 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8
Atopic dermatitis (AD) is a chronic inflammatory disease caused by immune dysregulation. Meanwhile, the supernatant of lactic acid bacteria (SL) was recently reported to have anti-inflammatory effects. In addition, HaCaT keratinocytes stimulated by tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) are widely used for studying AD‐like responses. In this study, we evaluated the antiinflammatory effects of SL from lactic acid bacteria (LAB) on TNF-α/IFN-γ-induced HaCaT keratinocytes, and then we investigated the strains’ probiotic properties. SL was noncytotoxic and regulated chemokines (macrophage-derived chemokine (MDC) and thymus and activation-regulated chemokine (TARC)) and cytokines (interleukin (IL)‐4, IL‐5, IL‐25, and IL‐33) in TNF-α/IFN-γ-induced HaCaT keratinocytes. SL from Lacticaseibacillus rhamnosus MG4644, Lacticaseibacillus paracasei MG4693, and Lactococcus lactis MG5474 decreased the phosphorylation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK). Furthermore, the safety of the three strains was demonstrated via hemolysis, bile salt hydrolase (BSH) activity, and toxicity tests, and the stability was confirmed under simulated gastrointestinal conditions. Therefore, L. rhamnosus MG4644, L. paracasei MG4693, and Lc. lactis MG5474 have potential applications in functional food as they are stable and safe for intestinal epithelial cells and could improve atopic inflammation.
한국인 여성 및 식품 유래 유산균의 생리적 특성 및 지방분화 억제효과
강창호(Chang-Ho Kang),정율아(Yulah Jeong),한설화(Seul Hwa Han),김진성(Jin-Seong Kim),김용경(YongGyeong Kim),박혜민(Hye Min Park),최수임(Soo-Im Choi),백남수(Nam-Soo Paek) 한국생물공학회 2018 KSBB Journal Vol.33 No.3
Obesity is the most common health problem in developed countries and is considered a significant risk factor for many major human diseases. This study aimed to evaluate the inhibitory effect of lactic acid bacteria isolated from the human vagina and shellfish on adipocyte differentiation in 3T3-L1 preadipocytes. The activity of separated 221 strains on pancrease lipase was measured and 15 strains were first screened. The level of adipogenesis of these strains in the 3T3-L1 cells was measured by Oil Red O staining assay. Five strains including Lactobacillus plantarum (2 strains) and L. fermentum (3 strains) showed good adipocyte differentiation inhibitory activity. Also, the selected strains were resistant to bile acid up to 3% and their autoaggregation rates were as high as 50%. These Lactobacillus strains with probiotics potential may be useful for prevention or treatment of obesity, but further in vitro and in vivo studies on these strains are still required.
Antioxidant and Probiotic Properties of Lactobacilli and Bifidobacteria of Human Origins
Hyemin Kim,Jin-Seong Kim,YongGyeong Kim,Yulah Jeong,Ji-Eun Kim,백남수,강창호 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3
Oxidative stress can cause various diseases including inflammation, neurological disorders, cancer, diabetes, and cardiovascular diseases. Due to the current search for natural antioxidants, probiotics have received increasing scientific interest and are facing a growing industrial demand. Although various strains of lactobacilli and bifidobacteria are currently used in numerous health food supplements, their antioxidative activities have been relatively poorly identified. Therefore, in this work, we evaluated the in vitro effect of antioxidative activities (through assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical scavenging) and probiotic functional properties (cell viability in a simulated gastrointestinal tract, enzyme production, carbohydrate availability, and safety assessments) of Lactobacillus spp. and Bifidobacterium spp. isolated from human origins. From the nitric oxide (NO) assay screening, four strains (Bifidobacterium animalis subsp. lactis MG741, B. breve MG729, L. reuteri MG505, and L. rhamnosus MG316) were selected based on the yield amount of ferment productivity (> × 1010 CFU/g) and showed high antioxidant activities ranging from 22.2% to 38.2% in DPPH free radical scavenging, and 50.0% to 93.6% in ABTS radical scavenging. Regarding their functional properties as probiotics, these four strains were resistant to simulated gastric (pH 3 and 4) and intestinal fluids (pH 7 and 8), and showed potential for the promotion of health based on hemolysis, auto-aggregation, antibiotic susceptibility, enzyme production, and biochemical profiles. Altogether, our results showed that the selected probiotic strains may be good candidates as food ingredients to mitigate oxidative stress-related symptoms.
전통발효식품에서 분리한 유산균의 항균활성 및 프로바이오틱스 기능성 연구
강창호(Chang-Ho Kang),한설화(Seul Hwa Han),김용경(YongGyeong Kim),정율아(Yulah Jeong),백남수(Nam-Soo Paek) 한국생물공학회 2017 KSBB Journal Vol.32 No.3
The aim of this study was to investigate probiotic characteristics and fermentation profile of selected lactic acid bacteria (LAB) isolated from traditional fermented foods. Antibacterial activity against various pathogens, acid and bile salt tolerance, cell hydrophobicity, and antibiotic resistance were examined. 16S rRNA sequencing was carried out to identify eight presumptive LAB isolates. In general, all identified LAB (Enterococcus faecium MG89-2, Lactobacillus plantarum MG207, L. paracasei MG310, L. casei MG311, Streptococcus thermophilus MG510, L. bulgaricus MG515, L. helveticus MG585, and L. fermentum MG590) showed strong antimicrobial activity. Also, the selected strains were resistant to bile acid up to 3% and their autoaggregation rates were as high as 60%. All selected strains were sensitive to chloramphenicol, tetracycline, and ampicillin, whereas resistant to nalidixic acid and kanamycin.
한국인 장관에서 분리한 유산균의 항균활성 및 프로바이오틱스 특성 연구
강창호(Chang-Ho Kang),김용경(YongGyeong Kim),한설화(SeulHwa Han),정율아(Yulah Jeong),백남수(Nam-Soo Paek) 한국생물공학회 2017 KSBB Journal Vol.32 No.2
The purpose of this study was to investigate the probiotic properties of lactic acid bacterial strains isolated from human feces. The properties were tested on the basis of guideline for probiotic selection protocol such as tolerance for acid or bile salt, autoaggregation, antibiotic resistance, and antimicrobial activity. Total 25 lactic acid bacteria were isolated from human feces, and their antibacterial activity was tested against Staphylococcus aureus, Escherichia coli, E. coli O157:H7, Vibrio parahaemolyticus, V. alginolyticus using an agar diffusion assay. Among them, 4 selected strains were identified by analysis of their 16S rRNA, as Lactobacillus rhamnosus MG316, L. acidophilus MG501, L. reuteri MG505, and L. gasseri MG570. Results show that resistance to low pH and bile salts. Also, the selected strains were resistant to bile acid up to 3% and their autoaggregation rates were as high as 60%. All strains tested were resistance to nalidixic acid and kanamycin.
Trung Hau Nguyen,YongGyeong Kim,Jin-Sung Kim,Yulah Jeong,Hye Min Park,Jin Woo Kim,Ji-Eun Kim,Hyemin Kim,Nam-Soo Paek,강창호 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.2
To expect the positive physiological functions from probiotic consumption, the lactic acid bacteria should survive, partially, in the gastrointestinal (GI) tract. This study aimed to evaluate the viable stability of probiotic bacterial cells (Lactobacillus plantarum MG989, L. fermentum MG901, Streptococcus thermophilus MG5140, Lactococcus lactis MG5125, and Enterococcus faecium MG89-2) that were coated with sodium alginate and pumpkin powder (SP) by comparing their resistance in simulated intestinal fluid (with pancreatin) and simulated gastric fluid (with pepsin) in vitro with that of the non-coated free cells. The viable stability was determined by counting cells with colony forming unit (CFU) from agar plate culture of SP coated and non-coated free cells in simulated GI conditions. Survival rate enhanced up to 28.7% and 14.0% in the condition of simulated gastric fluid and simulated intestinal fluid, respectively. The results showed that the SP coated cells exhibited considerably greater resistance to the simulated gastric fluid than the activated cells (p < 0.001), showing that the SP coating may enhance the survival of probiotic bacteria after consumption during their transit through the GI tract after freeze-drying process.
유산균의 생존율 향상을 위한 동결건조보호제로서 호박분말의 효과
강창호(Chang-Ho Kang),김용경(YongGyeong Kim),한설화(Seul Hwa Han),김진성(Jin-Seong Kim),정율아(Yulah Jeong),백남수(Nam-Soo Paek) 한국생물공학회 2017 KSBB Journal Vol.32 No.4
Food-grade protective agents such as sucrose, skim milk, and pumpkin powder were studied for their ability to improve the viability of freeze-dried lactic acid bacteria, including Lactobacillus plantarum MG989, L. fermentum MG901, Streptococcus thermophilus MG5139, Lactococcus lactis MG534, Enterococcus faecium MG89-2, and Bifidobacterium animalis ssp. lactis MG741. The best results were obtained with 5% sucrose contained 10% skim milk and 2% pumpkin powder; approximately 43.1 to 86.6% cell viability was observed during the freeze-drying process. During accelerated storage of freeze-dried lactic acid bacteria, those survival rate decrease rapidly, reaching 9.8~35.2% in 1 month. As a result, the group (5% sucrose, 10% skim milk, and 2% pumpkin powder) showed the protective effect much higher by 639.5% than the control group (5% sucrose and 10% skim milk). These results suggested that pumpkin powder was good candidate as a potential cryoprotectant agent.