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Antioxidant and Probiotic Properties of Lactobacilli and Bifidobacteria of Human Origins
Hyemin Kim,Jin-Seong Kim,YongGyeong Kim,Yulah Jeong,Ji-Eun Kim,백남수,강창호 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3
Oxidative stress can cause various diseases including inflammation, neurological disorders, cancer, diabetes, and cardiovascular diseases. Due to the current search for natural antioxidants, probiotics have received increasing scientific interest and are facing a growing industrial demand. Although various strains of lactobacilli and bifidobacteria are currently used in numerous health food supplements, their antioxidative activities have been relatively poorly identified. Therefore, in this work, we evaluated the in vitro effect of antioxidative activities (through assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical scavenging) and probiotic functional properties (cell viability in a simulated gastrointestinal tract, enzyme production, carbohydrate availability, and safety assessments) of Lactobacillus spp. and Bifidobacterium spp. isolated from human origins. From the nitric oxide (NO) assay screening, four strains (Bifidobacterium animalis subsp. lactis MG741, B. breve MG729, L. reuteri MG505, and L. rhamnosus MG316) were selected based on the yield amount of ferment productivity (> × 1010 CFU/g) and showed high antioxidant activities ranging from 22.2% to 38.2% in DPPH free radical scavenging, and 50.0% to 93.6% in ABTS radical scavenging. Regarding their functional properties as probiotics, these four strains were resistant to simulated gastric (pH 3 and 4) and intestinal fluids (pH 7 and 8), and showed potential for the promotion of health based on hemolysis, auto-aggregation, antibiotic susceptibility, enzyme production, and biochemical profiles. Altogether, our results showed that the selected probiotic strains may be good candidates as food ingredients to mitigate oxidative stress-related symptoms.
Trung Hau Nguyen,YongGyeong Kim,Jin-Sung Kim,Yulah Jeong,Hye Min Park,Jin Woo Kim,Ji-Eun Kim,Hyemin Kim,Nam-Soo Paek,강창호 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.2
To expect the positive physiological functions from probiotic consumption, the lactic acid bacteria should survive, partially, in the gastrointestinal (GI) tract. This study aimed to evaluate the viable stability of probiotic bacterial cells (Lactobacillus plantarum MG989, L. fermentum MG901, Streptococcus thermophilus MG5140, Lactococcus lactis MG5125, and Enterococcus faecium MG89-2) that were coated with sodium alginate and pumpkin powder (SP) by comparing their resistance in simulated intestinal fluid (with pancreatin) and simulated gastric fluid (with pepsin) in vitro with that of the non-coated free cells. The viable stability was determined by counting cells with colony forming unit (CFU) from agar plate culture of SP coated and non-coated free cells in simulated GI conditions. Survival rate enhanced up to 28.7% and 14.0% in the condition of simulated gastric fluid and simulated intestinal fluid, respectively. The results showed that the SP coated cells exhibited considerably greater resistance to the simulated gastric fluid than the activated cells (p < 0.001), showing that the SP coating may enhance the survival of probiotic bacteria after consumption during their transit through the GI tract after freeze-drying process.
다양한 환경에서의 탄산칼슘 생성 균주 분리 및 특성 연구
김용경(YongGyeong Kim),강창호(Chang-Ho Kang),오수지(Soo Ji Oh),소재성(Jae-Seong So) 한국생물공학회 2014 KSBB Journal Vol.29 No.5
Microbially induced calcite precipitation is a naturally occurring biological process in which microbes produce calcite on the surface of the microorganisms by urease activity. In order to collect calcite forming bacteria (CFB) in Korea, we isolated 343 putative CFB strains from various environments over three year period (2011~2013) and selected 100 CFB strains. Average of calcite productivity was 10.56 mg/ mL. And average of ammonium concentration by urease activity was 8.00 μM. Two useful CFB strains of the others were analyzed by 16S rRNA and identified as Sporosarcina sp. and Viridibacillus arenosi. The CFB strains presented in this study are indigenous microorganisms in Korea and they are expected to be applicable to a variety of environments in the country.
한국 여성의 질에서 분리한 유산균의 Gardnerella vaginalis에 대한 항균효과 및 특성 규명
김용경(YongGyeong Kim),강창호(Chang-Ho Kang),신유진(YuJin Shin),백남수(Nam-Soo Paek),소재성(Jae-Seong So) 한국생물공학회 2015 KSBB Journal Vol.30 No.5
Bacterial vaginosis (BV) is caused by microbial imbalance of the vaginal ecosystem and overgrowth of anaerobic bacteria. The antibiotic treatment often results in very high recurrence of BV because it disturbs the vaginal ecosystem. The high recurrence rates suggest a need for alternative therapeutic methods and probiotics are being recognized as alternative or additional treatment method for BV. The purpose of this study was to investigate how human vaginal isolates of Lactobacillus spp. inhibit the BV-associated pathogen Gardnerella vaginalis. Results show that selected strains significantly reduced the viability of G. vaginalis. Among these selected strains KLB410 and KLB416 were further selected based on acid/bile tolerance and identified through 16S rRNA gene sequencing being Lactobacillus plantarum. Further studies are underway to demonstrate that the selected strain can be applied as potential probiotics for recovering vaginal ecosystem.
Kang, Chang-Ho,Shin, YuJin,Kim, WooRi,Kim, YongGyeong,Song, KiCheol,Oh, Eun-Gyoung,Kim, SuKyung,Yu, HongSik,So, Jae-Seong Springer-Verlag 2016 Environmental Science and Pollution Research Vol.23 No.1
<P>Vibrio parahaemolyticus is the most prevalent gastroenteritis-causing pathogen in Korea and in some other Asian countries. It is frequently found in oysters and other seafood. This study monitored changes in the prevalence of V. parahaemolyticus and environmental parameters in oyster aquaculture environments in Korea. From June to October 2014, we tested oysters (Crassostrea gigas) from shellfish-harvesting areas off the west coast of Korea. These 71 isolates were the sum of 16 (22.5 %), 19 (26.8 %), 23 (32.4 %), and 13 (18.3 %) isolates collected in July, August, September, and October, respectively. These 71 isolates had the following profiles of resistance against 16 antibiotics: all isolates were resistant to ampicillin and vancomycin, and 52.2, 50.7, and 50.7 % of isolates exhibited resistance to cephalothin, rifampin, and streptomycin, respectively. PCR analysis for the presence of the species-specific toxR gene confirmed that 38 (53.5 %) of the total 71 isolated strains were positive for V. parahaemolyticus. In PCR analysis for virulence of V. parahaemolyticus, of the 71 isolates tested in the present study, only 38 (53.5 %) were positive for the trh virulence gene and 71 (100 %) was negative for the tdh virulence gene.</P>
한국인 장관에서 분리한 유산균의 항균활성 및 프로바이오틱스 특성 연구
강창호(Chang-Ho Kang),김용경(YongGyeong Kim),한설화(SeulHwa Han),정율아(Yulah Jeong),백남수(Nam-Soo Paek) 한국생물공학회 2017 KSBB Journal Vol.32 No.2
The purpose of this study was to investigate the probiotic properties of lactic acid bacterial strains isolated from human feces. The properties were tested on the basis of guideline for probiotic selection protocol such as tolerance for acid or bile salt, autoaggregation, antibiotic resistance, and antimicrobial activity. Total 25 lactic acid bacteria were isolated from human feces, and their antibacterial activity was tested against Staphylococcus aureus, Escherichia coli, E. coli O157:H7, Vibrio parahaemolyticus, V. alginolyticus using an agar diffusion assay. Among them, 4 selected strains were identified by analysis of their 16S rRNA, as Lactobacillus rhamnosus MG316, L. acidophilus MG501, L. reuteri MG505, and L. gasseri MG570. Results show that resistance to low pH and bile salts. Also, the selected strains were resistant to bile acid up to 3% and their autoaggregation rates were as high as 60%. All strains tested were resistance to nalidixic acid and kanamycin.
유산균의 생존율 향상을 위한 동결건조보호제로서 호박분말의 효과
강창호(Chang-Ho Kang),김용경(YongGyeong Kim),한설화(Seul Hwa Han),김진성(Jin-Seong Kim),정율아(Yulah Jeong),백남수(Nam-Soo Paek) 한국생물공학회 2017 KSBB Journal Vol.32 No.4
Food-grade protective agents such as sucrose, skim milk, and pumpkin powder were studied for their ability to improve the viability of freeze-dried lactic acid bacteria, including Lactobacillus plantarum MG989, L. fermentum MG901, Streptococcus thermophilus MG5139, Lactococcus lactis MG534, Enterococcus faecium MG89-2, and Bifidobacterium animalis ssp. lactis MG741. The best results were obtained with 5% sucrose contained 10% skim milk and 2% pumpkin powder; approximately 43.1 to 86.6% cell viability was observed during the freeze-drying process. During accelerated storage of freeze-dried lactic acid bacteria, those survival rate decrease rapidly, reaching 9.8~35.2% in 1 month. As a result, the group (5% sucrose, 10% skim milk, and 2% pumpkin powder) showed the protective effect much higher by 639.5% than the control group (5% sucrose and 10% skim milk). These results suggested that pumpkin powder was good candidate as a potential cryoprotectant agent.
전통발효식품에서 분리한 유산균의 항균활성 및 프로바이오틱스 기능성 연구
강창호(Chang-Ho Kang),한설화(Seul Hwa Han),김용경(YongGyeong Kim),정율아(Yulah Jeong),백남수(Nam-Soo Paek) 한국생물공학회 2017 KSBB Journal Vol.32 No.3
The aim of this study was to investigate probiotic characteristics and fermentation profile of selected lactic acid bacteria (LAB) isolated from traditional fermented foods. Antibacterial activity against various pathogens, acid and bile salt tolerance, cell hydrophobicity, and antibiotic resistance were examined. 16S rRNA sequencing was carried out to identify eight presumptive LAB isolates. In general, all identified LAB (Enterococcus faecium MG89-2, Lactobacillus plantarum MG207, L. paracasei MG310, L. casei MG311, Streptococcus thermophilus MG510, L. bulgaricus MG515, L. helveticus MG585, and L. fermentum MG590) showed strong antimicrobial activity. Also, the selected strains were resistant to bile acid up to 3% and their autoaggregation rates were as high as 60%. All selected strains were sensitive to chloramphenicol, tetracycline, and ampicillin, whereas resistant to nalidixic acid and kanamycin.
Kang, Chang-Ho,Oh, Soo Ji,Shin, YuJin,Kim, YongGyeong,Oh, Eun-Gyoung,So, Jae-Seong Taylor Francis 2017 Urban water journal Vol.14 No.1
<P>The bacteriological pollution of water is a serious global environmental issue. The pollution of water by human and animal wastes can be a source of hazardous pathogens, which may pose serious health risks. In this study, we evaluated the microbiological water quality in the southern Ganghwado Bay area, South Korea. A total of 30 samples ( 14 discharge water samples from the drainage area and 16 sea water samples from the adjacent area) were analyzed for the concentrations of total coliforms ( TC) and fecal coliforms ( FC) in November 2013. The highest concentration of FC ( 28,000 MPN/100 mL) was found at site L1 ( inland). The highest mean concentrations of coliforms were found at sites W9 ( sea water) and W11 ( sea water), with TC and FC concentrations of 33 MPN/100 mL. In conclusion, site L1 located near W9 and W11 is clearly identified as the main source of pollution at Dongmak Beach.</P>