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Saravanakumar, Kandasamy,Chelliah, Ramachandran,MubarakAli, Davoodbasha,Jeevithan, Elango,Oh, Deog-Hwan,Kathiresan, Kandasamy,Wang, Myeong-Hyeon Elsevier 2018 International journal of biological macromolecules Vol.118 No.2
<P><B>Abstract</B></P> <P>This paper reports the synthesis of chitosan nanoparticles (T-CSNPs) using the fungal enzyme of <I>Trichoderma harzianum</I> and its biocompatibility, antioxidant and bactericidal properties. The T-CSNPs synthesis was confirmed by absorbance at 280 nm using UV–Vis spectrophotometer. T-CSNPs were of spherical shape, as evident by field emission transmission electron microscopic (FETEM) analysis, and the average size of T-CSNPs was 90.8 nm, as calculated using particle size analyzer (PSA). The functional groups showed modifications of chitosan in T-CSNPs as evident by fourier-transform infrared spectroscopic (FTIR) analysis. T-CSNPs were found soluble at the wide range of pH, showing 100% solubility at pH 1–3 and 72% at pH 10. The T-CSNPs exhibited antioxidant property in a dose-dependent manner with pronounced activity at 100 mg·mL<SUP>−1</SUP>. The T-CSNPs also showed bactericidal activity against <I>Staphylococcus aureus</I> and <I>Salmonella enterica</I> Typhimurium by causing detrimental effects on bacterial cells. The T-CSNPs (50 μg·mL<SUP>−1</SUP>) did not display any cytotoxic effect on murine fibroblast NIH-3T3 cells, as evident by cell viability and acridine orange/ethidium bromide staining assays, which confirmed biocompatibility of the nanoparticles. This work suggested further investigations on the utilization of the mycosynthesized nanochitosan in biomedical applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Synthesized the bioactive chitosan nanoparticles (T-CSNPs) using fungal extract of <I>Trichoderma</I> sp. </LI> <LI> Determined the molecular weight of enzyme in the fungal extract </LI> <LI> Enhanced the antioxidant and the bactericidal activities of T-CSNPs </LI> <LI> Assessed the biocompatibility of the T-CSNPs through cytotoxicity assay against NIH-3T3 cells </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>