Effect of Light with Different Wavelengths on Nostoc flagelliforme Cells in Liquid Culture

( Yu Jie Dai ),( Jing Li ),( Shu Mei Wei ),( Nan Chen ),( Yu Peng Xiao ),( Zhi Lei Tan ),( Shi Ru Jia ),( Nan Nan Yuan ),( Ning Tan ),( Yi Jie Song ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4

The effects of lights with different wavelengths on the growth and the yield of extracellular polysaccharides of Nostoc flagelliforme cells were investigated in a liquid cultivation. N. flagelliforme cells were cultured for 16 days in 500 ml conical flasks containing BG11 culture medium under 27 μmol·m-2·s-1 of light intensity and 25oC on a rotary shaker (140 rpm). The chlorophyll a, phycocyanin, allophycocyanin, and phycoerythrin contents in N. flagelliforme cells under the lights of different wavelengths were also measured. It was found that the cell biomass and the yield of polysaccharide changed with different wavelengths of light. The biomass and the yield of extracellular polysaccharides under the red or violet light were higher than those under other light colors. Chlorophyll a, phycocyanin, and allophycocyanin are the main pigments in N. flagelliforme cells. The results showed that N. flagelliforme, like other cyanobacteria, has the ability of adjusting the contents and relative ratio of its pigments with the light quality. As a conclusion, N. flagelliforme cells favor red and violet lights and perform the complementary chromatic adaptation ability to acclimate to the changes of the light quality in the environment.

Taguchi Approach for Anti-heat Stress Prescription Compatibility in Mice Spleen Lymphocytes In Vitro

Xiao-yu Zhu,Gui-lin Cheng,Feng-hua Liu,Jin Yu,Yu-jie Wang,Tong-quan Yu,Jian-qin Xu,Ming Wang 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7

Heat stress (HS) may induce immunosuppression as well as inhibit the proliferation of lymphocytes. This study evaluated the effects on immune function of our prescription on splenic lymphocytes under HS as well as its compatibility. The effects of four herbal extracts from Agastache rugosa, Atractylodes lancea, Cortex Phellodendri, and Gypsum Fibrosum on heat treated splenic lymphocytes were investigated and the compatibility of the prescription was also explored by using the Taguchi method. This study revealed changes in proliferation by traditional Chinese medicines of splenic lymphocytes after HS. Proliferation in the HS group was significantly lower than the control group. Under HS, the effects of higher concentrations of Agastache rugosa (100 and 200 μg/mL), Atractylodes lancea (100 and 200 μg/mL), Cortex Phellodendri (50 and 100 μg/mL) and Gypsum Fibrosum (100 and 200 μg/mL) caused a significant increase on ConA/LPS-induced proliferation of lymphocytes than lower concentrations. We, therefore, conclude that the prescription of traditional Chinese medicines may recover splenic lymphocytes from the immunosuppression induced by HS. The Taguchi design, which allows rapid and high efficiency for the selection of the best conditions for our prescription on HS-treated splenic lymphocytes, demonstrated that Agastache rugosa (200 μg/mL), Atractylodes lancea (200 μg/mL), Cortex Phellodendri (100 μg/mL) and Gypsum Fibrosum (100 μg/mL)were the optimal conditions for the prescription. The validation experiment confirmed that our composition in optimum extraction conditions enhanced effects on ConA or LPS-stimulated lymphocytes under HS. The results showed that the Taguchi optimization approach is a suitable method for optimization of the composition of prescription.

Clinicopathological Features and Prognosis of Gastroenteropancreatic Neuroendocrine Tumors: Analysis from a Single-institution

Zeng, Yu-Jie,Liu, Lu,Wu, Heng,Lai, Wei,Cao, Jie-Zhi,Xu, He-Yang,Wang, Jie,Chu, Zhong-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: The gastroenteropancreatic neuroendocrine neoplasm (GEP-NEN) is the most common type of neuroendocrine neoplasm. We summarized data in our centre to investigate the clinicopathological features, diagnostic methods, therapeutic approaches and prognosis for this neoplasm to increase knowledge of this disease in Asian populations. Method: A total of 122 patients treated at Sun Yet-san Memorial Hospital of Sun Yat-sen University between January 2000 and December 2011 were analyzed retrospectively. Results: Pancreas was the most common site of involvement (65/122, 53.3%); this disease has no special symptoms; positive rates of chromogranin A (CgA) and synaptophysin (Syn) were 81.1% and 87.7%, respectively. The positive rate of Syn had statistical difference among the three grades, but not CgA. Some 68 patients had G1 tumors, 32 G2 tumors and 22 G3 tumors, and Chi-square test showed that higher grading was correlated with worse prognosis (${\chi}^2=32.825$, P=0.0001). A total of 32 patients presented with distant metastasis, and 8 cases emerged during following up. Cox proportional hazards regression modeling showed that the tumor grade (P=0.01), lymphatic metastasis (P=0.025) and distant metastasis (P=0.031) were predictors of unfavorable prognosis. The overall 5-year survival rate was 39.6%, the 5-year survival rate of G1 was 55.7%, and the G2 and G3 were 34.2% and 0%, respectively. Conclusions: The incidence of gastroenteropancreatic neuroendocrine tumors has risen over the last 12 years. All grades of these diseases metastasize readily, and further research regarding the treatment of patients after radical surgery is needed to prolong disease-free survival.

Bioaccumulation of Arsenic in Recombinant Escherichia coli Expressing Human Metallothionein

Yu-Jie Su,Jian-Qun Lin,Jian-Qiang Lin,Dong-Hui Hao 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.5

The recombinant Escherichia coli(E.coli) expressing human hepatic metallothionein_IA (hMT_IA) was constructed for bioaccumulation of Arsenic (As). The gene sequence of hMT_IA was modified for codon preference of E.coli and synthesized using chemical method. The vector of pGEX_4T_1 was used=and hMT_IA was expressed as the fusion protein with glutathione S-transferase (GST) tag. The bioaccumulation capability of arsenite compounds As(III) of the recombinant E.coli increased more than 3-fold from 76.3 to 319.6 μg/g dry cells compared with the control. The conditions of 50 μM of As(III) and low pHs were optimal for As(III) bioaccumulation. The heavy metals of Cd, Hg, and Zn inhibited As(III) bioaccumulation. The bioaccumulation reached 70% of the saturated value within 1 h. The recombinant E.coli will be useful in bioremediation of arsenic or other kinds of heavy metal contaminated water. The recombinant Escherichia coli(E.coli) expressing human hepatic metallothionein_IA (hMT_IA) was constructed for bioaccumulation of Arsenic (As). The gene sequence of hMT_IA was modified for codon preference of E.coli and synthesized using chemical method. The vector of pGEX_4T_1 was used=and hMT_IA was expressed as the fusion protein with glutathione S-transferase (GST) tag. The bioaccumulation capability of arsenite compounds As(III) of the recombinant E.coli increased more than 3-fold from 76.3 to 319.6 μg/g dry cells compared with the control. The conditions of 50 μM of As(III) and low pHs were optimal for As(III) bioaccumulation. The heavy metals of Cd, Hg, and Zn inhibited As(III) bioaccumulation. The bioaccumulation reached 70% of the saturated value within 1 h. The recombinant E.coli will be useful in bioremediation of arsenic or other kinds of heavy metal contaminated water.

Preliminary Proteomic Analysis of Indomethacin’s Effect on TumorTransplanted with Colorectal Cancer Cell in Nude Mice

Yu-Jie Wang,Hong-Mei Wang,Gui-Ying Zhang,Zhi-Qiang Xiao,Zhu-Chu Chen 한국생화학분자생물학회 2006 BMB Reports Vol.39 No.2

The Regulation of Root Pruning and Soil Water Content to Apple Leaf Water Use Efficiency

Yu-Ling Jie,Hong-Qiang Yang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Edward Young’s Night Thoughts IX: A Reworking of Spinoza and Locke in Perceptions of Divinity

Yu Jie-ae 한국문학과종교학회 2020 문학과종교 Vol.25 No.4

본 논문은 에드워드 영이 『밤의 명상 제 9편』(1745)에서 스피노자와 로크의 사상을 토대로 하여 시적 화자가 자연 세계에 내재하는 신의 존재를 인식해 가는 과정을 다루고 있음을 보여준다. 감수성의 시대에 살았던 에드워드 영은 이 작품을 통하여 당대의 그 어느 작가보다도 자연 속에 드러나는 신성성에 대한 인간 내면의 경험을 진지하고도 깊이 있게 다루고 있다. 그는 그동안 문학 비평 가들이 간과해온 스피노자와 로크의 사상을 효과적으로 활용하고 있으며, 이는 시적 화자가 추상적이고 심오한 종교 문제를 등한시하며 덧없는 이 세상의 가치를 추구하는, 당대의 지성인을 대표하는 로렌조로 하여금 신성에 대한 인식을 갖도록 역설하는데 중요한 바탕이 된다. 이러한 시적 화자의 의도는 송시(ode) 라는 역동적인 시의 형식을 통하여 효율적으로 전달되고 있다. 이와 같은 시의 형태와 더불어 작가는 두 사상가의 이론을 작품에 반영함으로써 불멸의 자연에 드러나는 신의 존재를 인간은 사유할 수 있다는 점을 강조하고 있다. 아울러 에드워드 영은 현실 중심적이고 계산적인 인간의 이성 이외에 신성성을 인지하는 내면의 주관적 경험을 표현함으로써 낭만주의 시의 전초를 알려주고 있다. This article examines how Edward Young’s Night Thoughts IX reshapes the key concepts of Baruch Spinoza and John Locke while deploying the speaker’s perceptions of the Almighty in human domains. At the age of sensibility, it was Young among his contemporary writers, who seriously utilizes, in his contemplative ode, divine experiences of external circumstances. His reworking of the two philosophers enables the speaker to effectively deliver his spiritual awareness of divinity to Lorenzo, who is a contemporary iconic figure ignoring immaterial revelations in the surrounding world. The essay scrutinizes how Young’s allusion to Spinoza and Locke contributes to enhancing the capability of one’s mind to realize the diverse manifestations of deity in permanent natural landscapes.

Matthew Arnold’s “The Church of Brou”: Precariousness, Distress, and a Pursuit of Spiritual Enhancement

Jie-Ae Yu 한국영미어문학회 2019 영미어문학 Vol.- No.135

This article investigates how Arnold’s “The Church of Brou” manifests his thematic concerns with vicissitude of human felicity, tribulation necessitated by it, and his resilient search for attaining spiritual fortification so as to overcome such apparent sensitivity to predicament. In the intertwined structure of three sections, “The Castle,” “The Church,” and “The Tomb,” the author unravels the ironic loss and deprivation of one’s longing for the private and religious realms of ideal goals. As in his early poems, Arnold sustains his prominent issue of man’s tribulation which registers dilemmatic conflicts between his stoical resolution and its opposing threats. However, the unique features of “The Church of Brou” underlie its diverse range of treating poetic objects which bring about the paradoxical facets of mutable occurrences. The poem prefigures subsequent elegiac poems and prose works in which Arnold embodies an ardent quest for immortality of man’s spiritual faculty, whereby he can liberate himself from the sustaining perceptions of the irresolvable predicament.

Matthew Arnold’s Empedocles on Etna: Adversity, Contemplation, and Territory of Human Resolution

Yu, Jie-Ae 신영어영문학회 2020 신영어영문학 Vol.75 No.-

This article examines how Matthew Arnold’s dramatic poem, Empedocles on Etna, embodies the protagonist’s internal dialogue with his mind and a spiritual awakening in reacting to the irresistible moments of his affliction imposed by himself and society. Setting aside the spiritual problems of the Victorian era, the writer refers back to the ancient Sicilian philosopher to investigate the disregarded root and cause of human suffering, his resilient power to cope with it, and the boundary of man’s determination in resolving the distressing matter. Arnold’s Empedocles on Etna reworks the nature of drama, whose chief feature underlies a severe conflict inherent in internal and external domains. Arnold brings forward the protagonist’s deep solitude where he reveals his dialogue with his own mind and frustrates to liberate himself from the heavy burden of grief and solitariness. He is hurled into a spiritual turbulence, yet his unswerving meditation drives him to the fire of crater which ignites his vibrant solution for rejuvenating man’s spiritual power and dignity.

Matthew Arnold’s Sohrab and Rustum : Unfurling Characterization and Thematic Manifestations

Yu, Jie-Ae 한국중앙영어영문학회 2021 영어영문학연구 Vol.63 No.4

This article examines how Arnold’s narrative poem Sohrab and Rustum manifests unique features of two main characters in reference to concepts of unknown irresistible power and man’s relentless defiance. This poem, published during the early stage of Arnold’s literary career in 1853, resonates Sophoclean concepts of the clash between fate and human pride. While dealing with Arnoldian awareness of the Greek dramatic allusion, this paper also highlights his anticipation of later figures, for instance, Thyrsis and poetic speakers who reflect the spirit of Sohrab and Rustum but show their own unique personalities. It also argues that Arnold’s characterization in Sohrab and Rustum establishes key concepts of the bounds of human wishes and enterprises against their adverse forces. While scrutinizing this aspect, the paper relates this aspect to Arnold’s earlier works. In addition, it demonstrates that his portrayals of the characters foreshadows a wide range of the subsequent construction of his literary oeuvre which associates with the speaker’s elegiac lyric sensitivity to the era of spiritual turmoil and the author’s dynamic portrayals of the paradox and irony of man’s life and the world.