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Irisin, a Novel Myokine, Regulates Glucose Uptake in Skeletal Muscle Cells via AMPK
Lee, Hye Jeong,Lee, Jung Ok,Kim, Nami,Kim, Joong Kwan,Kim, Hyung Ip,Lee, Yong Woo,Kim, Su Jin,Choi, Jong-Il,Oh, Yoonji,Kim, Jeong Hyun,Hwang, Suyeon-,Park, Sun Hwa,Kim, Hyeon Soo Endocrine Society 2015 Molecular endocrinology Vol.29 No.6
<P>Irisin is a novel myokine produced by skeletal muscle. However, its metabolic role is poorly understood. In the present study, irisin induced glucose uptake in differentiated skeletal muscle cells. It increased AMP-activated protein kinase (AMPK) phosphorylation and the inhibition of AMPK blocked glucose uptake. It also increased reactive oxygen species (ROS) generation. N-acetyl cysteine, a ROS scavenger, blocked irisin-induced AMPK phosphorylation. Moreover, irisin activated p38 MAPK in an AMPK-dependent manner. The inhibition and knockdown of p38 MAPK blocked irisin-induced glucose uptake. A colorimetric absorbance assay showed that irisin stimulated the translocation of glucose transporter type 4 to the plasma membrane and that this effect was suppressed in cells pretreated with a p38 MAPK inhibitor or p38 MAPK small interfering RNA. In primary cultured myoblast cells, irisin increased the concentration of intracellular calcium. STO-609, a calcium/calmodulin-dependent protein kinase kinase inhibitor, blocked irisin-induced AMPK phosphorylation, implying that calcium is involved in irisin-mediated signaling. Our results suggest that irisin plays an important role in glucose metabolism via the ROS-mediated AMPK pathway in skeletal muscle cells.</P>
기무라병으로 오진된, 말초혈액 호산구증가증을 동반한 역형성대세포림프종
신윤지 ( Yoonji Shin ),오지연 ( Jee Youn Oh ),이영석 ( Young Seok Lee ),민경훈 ( Kyung Hoon Min ),이승룡 ( Sung Yong Lee ),심재정 ( Jae Jeong Shim ),강경호 ( Kyung Ho Kang ),허규영 ( Gyu Young Hur ) 대한천식알레르기학회(구 대한알레르기학회) 2018 Allergy Asthma & Respiratory Disease Vol.6 No.2
Hypereosinophilia, defined as an absolute eosinophil count of >1,500/μL, can be caused by a number of allergic, infectious, paraneoplastic and neoplastic disorders. In cases of hypereosinophilia with lymphoid proliferation, pathological confirmation is essential to exclude either myeloid or lymphoid malignancy. A 38-year-old woman with both cervical lymphadenopathies and peripheral blood eosinophilia visited our clinic. She had already performed core biopsy of lymph nodes and diagnosed as Kimura disease at a regional hospital. At the time of our clinic visit, there were no palpable cervical lymph nodes. The blood test showed hypereosinophilia with a high total IgE level. There was no evidence of tissue infiltration of eosinophils except for duodenitis with eosinophilic infiltration. Based on these findings, she was diagnosed as Kimura disease. She treated with high-dose systemic corticosteroid (1 mg/kg) and additional immunosuppressants sequentially used cyclophosphamide and cyclosporine. However, her eosinophilia waxed and waned, and a left inguinal mass was newly found. Excisional biopsy findings showed large atypical lymphoid cells with numerous eosinophilis, and immunohistochemistry showed CD3+, CD20-, CD30+ and anaplastic lymphoma kinase (ALK). The final diagnosis was ALK-negative anaplastic large cell lymphoma. We report a case of anaplastic large cell lymphoma with marked peripheral eosinophilia misdiagnosed as Kimura disease. In the case of hypereosinophilia with lymphadenopathy, it is necessary to differentiate hematologic diseases through immunochemical staining. (Allergy Asthma Respir Dis 2018;6:131-134)
Orbital exenteration for a third eyelid gland carcinoma in a dog
Park, Yoonji,Kang, Seonmi,Jeong, Manbok,Park, Jungyun,Seo, Kangmoon The Korean Society of Veterinary Science 2018 大韓獸醫學會誌 Vol.58 No.4
A 13-year-old neutered male Poodle dog was presented with a third eyelid mass in the left eye. The dog had undergone local resection of the mass about a year prior in a private practice. On cytological examination, the mass was diagnosed as adenocarcinoma. Although lung and lymph node metastases were suspected, based upon the computed tomographic results, exenteration was performed to relieve chronic pain and to improve the dog's quality of life. Exenteration carried a good prognosis with no tumor recurrence until 1 year and 10 months after surgery, when local recurrence occurred near the left zygomatic arch.
여성경영주 농가의 농촌관광사업 참여가 농업경영 성과에 미치는 영향 분석
정용경(Jeong, Yongkyeong),황정임(Hwang, Jeongim),최윤지(Choi, Yoonji),최정신(Choi, Jungshin) 대한관광경영학회 2019 觀光硏究 Vol.34 No.8
인구 감소와 고령화가 심각한 농업·농촌에서 여성농업인의 역할은 매우 중요하다. 본 연구는 이러한 상황을 배경으로 여성농업인의 농업경영 특성과 농축산물 판매금액에 미치는 영향요인을 분석한다. 분석 결과, 여성농업인은 남성농업인에 비해 연령이 높았으며, 교육 수준이 낮았다. 농촌관광사업에의 참여는 남성농업인에 비해 낮았으며, 경작 면적이 남성의 1/2 수준인 만큼 농축산물 판매금액도 낮은 것으로 나타났다. 여성농업인 중에서도 젊을수록 교육수준이 높고 판매금액이 높았으며, 농촌관광사업에의 참여율이 높은 것으로 나타났다. 여성농업인의 농축산물 판매금액에 긍정적인 영향을 주는 경영주 특성 요인은 배우자의 존재, 높은 교육수준, 가구원 수가 많은 경우 등이었다. 농촌관광사업에 참여할수록 농축산물 판매금액이 높아졌으며, 그밖에 생산자 조직에의 참여, 농기계 보유, 정보화기기 활용 등도 농축산물 판매금액에 긍정적인 영향을 미치는 것으로 나타났다. The purpose of this study is to identify the agricultural management characteristics of female-managed farms and to analyze the factors affecting their agricultural total sales. Doing rural tourism business is considered as one of the main factors affecting agricultural management performance. The results show that female managers are older and have lower education levels. The female managers are doing rural tourism business less than male managers, and have smaller acreage and accordingly record less total sales. Younger female managers have higher education levels and higher sales record, and are doing rural tourism business more than older female managers. Having a spouse, high education level and number of households have a positive effect on agricultural performance. Also, doing rural tourism business, participating producer organization, having farming machinery and using information equipments for farming affect positively on agricultural performance.
Lee, Yoonji,Jeong, Lak Shin,Choi, Sun,Hyeon, Changbong American Chemical Society 2011 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.133 No.49
<P><I>S</I>-adenosylhomocysteine hydrolase (SAHH), a cellular enzyme that plays a key role in methylation reactions including those required for maturation of viral mRNA, is an important drug target in the discovery of antiviral agents. While targeting the active site is a straightforward strategy of enzyme inhibition, evidence of allosteric modulation of active site in many enzymes underscores the molecular origin of signal transduction. Information of co-evolving sequences in SAHH family and the key residues for functional dynamics that can be identified using native topology of the enzyme provide glimpses into how the allosteric signaling network, dispersed over the molecular structure, coordinates intra- and intersubunit conformational dynamics. To study the link between the allosteric communication and functional dynamics of SAHHs, we performed Brownian dynamics simulations by building a coarse-grained model based on the holo and ligand-bound structures. The simulations of ligand-induced transition revealed that the signal of intrasubunit closure dynamics is transmitted to form intersubunit contacts, which in turn invoke a precise alignment of active site, followed by the dimer–dimer rotation that compacts the whole tetrameric structure. Further analyses of SAHH dynamics associated with ligand binding provided evidence of both induced fit and population shift mechanisms and also showed that the transition-state ensemble is akin to the ligand-bound state. Besides the formation of enzyme-ligand contacts at the active site, the allosteric couplings from the residues distal to the active site are vital to the enzymatic function.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2011/jacsat.2011.133.issue-49/ja2066175/production/images/medium/ja-2011-066175_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja2066175'>ACS Electronic Supporting Info</A></P>
Park Saeyoung,Jeong Soyeon,Nam Yu Hwa,Yum Yoonji,Jung Sung-Chul 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6
BACKGROUND: Skeletal muscles play many important roles in the human body and any malfunction or disorder of the skeletal muscles can lead to a reduced quality of life. Some skeletal dysfunctions are acquired, such as sarcopenia but others are congenital. Duchenne muscular dystrophy (DMD) is one of the most common forms of hereditary muscular dystrophy and is caused by a deficiency of the protein, Dystrophin. Currently, there is no clear treatment for DMD, there are only methods that can alleviate the symptoms of the disease. Mesenchymal stem cells, including tonsil-derived mesenchymal stem cells (TMSCs) have been shown to differentiate into skeletal muscle cells (TMSC-myocyte) and can be one of the resources for the treatment of DMD. Skeletal muscle cell characteristics of TMSC-myocytes have been confirmed through changes in morphology and expression of skeletal muscle markers such as Myogenin, Myf6, and MYH families after differentiation. MEOTHDS: Based on these characteristics, TMSC-myocytes have been transplanted into mdx mice, a mouse model of DMD, to investigate whether they can help improve the symptoms of DMD. The red fluorescent protein gene was transduced into TMSC (TMSC-R) for tracking transplanted cells. RESULTS: Prior to transplantation (TP), it was confirmed whether TMSC-R-myocytes had the same differentiation potential as TMSC-myocytes. Increased expression of dystrophin and autophagy markers in the TP group compared with the sham group was confirmed in the gastrocnemius muscle 12 weeks after TP. CONCLUSION: These results demonstrate muscle regeneration and functional recovery of mdx via autophagy activation following TMSC-myocyte TP.