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Comparison between M. Avium and M. Intracellulae Infections in Lung Using HRCT Scoring System
( Sung Chul Park ),( Yong Chul Lee ),( So Ri Kim ),( Gong Yong Jin ),( Yang Keun Rhee ),( Heung Bum Lee ),( Seoung Ju Park ),( Chi Ryang Chung ),( Seung Yong Park ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
The prevalence of pulmonary diseases caused by non-tuberculous mycobacteria appears to be increasing worldwide. The predominant pathogen is M. Avium complex, which is comprised of M. Avium and M. Intracellulare and they have been considered to possess the similar characteristics. However, there is little information on the comparative data of their clinical and radiologic features. This retrospective analysis was performed on 188 patients with pulmonary infection by MAC complex diagnosed from January 2006 to December 2011 in a teaching Hospital. In both pulmonary infections with M. Avium and M. Intracelluae, the most common pulmonary comorbidities are COPD and previous history of pulmonary tuberculosis. In addition, the pathologic lesions by both pathogens were involved in right middle lobe and left lingular segments predominantly. Subcentimeter nodules, cyllindric bronchiectasis, and segmental or sub-segmental consolidation are representative radiologic findings in pulmonary infections with both species. Therapeutic responses are also similar after the standard treatment, although it seems to be more favor in M. M. Intracelluae (69%) than those in M. Avium (61%). These findings suggest that M. Avium and M. Intracellulae infection show similar clinical and radiologic features in the radiologic scoring system by HRCT.
Yong-Suk Ryang,Yoon-Kyung Cho,Jee-Aee IM,Kyu-Je Lee,Insik Kim,Ho-Joong Sung 대한의생명과학회 2004 Biomedical Science Letters Vol.10 No.3
This experiment was performed to examine the in vitro and in vivo affects of the two different haplotype strains of mice, BALB/c and C3H/HeN infected with Echinostoma hortense, and the manifestation of the profiles of cytokine in the splenocytes. In the in vitro experiment, the two mice's splenocytes were divided and stimulated with antigen of crude extracts and the antigen of excretory and secretory products of an adult warm and the manifestation of cytokine mRNA was verified with RT-PCR. As a result, the two different strains of mice both strongly manifested the Th2 cytokine rather than the Th1 cytokine and in the case of the Th2 cytokine, the BALB/c mice manifested more strongly than the C3H/HeN mice. In the experiment using the ELISA method, the protein cytokine manifestation had the same result as the mRNA experiment. In the in vivo experiment, the mice was infected via oral route with the metacercaria of the Echinostoma hortense and the manifestation of cytokine was verified by RT-PCR and ELISA and the results were the same as the in vitro experiment. Therefore, in the two strains of BALB/c and C3H/HeN, the C3H/HeN showed a higher susceptivity to the Echinostoma hortense.
Primary Thoracic Sarcoma in Anterior Mediastinum
( Sung Chul Park ),( So Ri Kim ),( Yong Chul Lee ),( Na Ri Lee ),( Yang Keun Rhee ),( Heung Bum Lee ),( Seoung Ju Park ),( Chi Ryang Chung ),( Seung Yong Park ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
Most common anterior mediastinal mass is thymoma, lymphoma and teratoma. Sarcomas are rare connective tissue malignant neoplasm which can be affected various locations, most frequently occur in the extremities. In fact, primary mediastinal sarco-mas are extremely uncommon, less than 2% of all soft tissue sarcoma cases. The majority of primary thoracic sarcomas are originated from chest wall, pleural spaces and nerve sheath. Therefore, the primary thoracic sarcomas involved in the lung parenchyma are rarer with poor information regarding the proper management and its outcomes. Herein, we report a rare and interesting case of primary thoracic high-grade sarcoma occupying anterior mediastinum and left lower lobe of the lung of a 35-years-old man, with reviewing the previous reports and literatures.
Effects of Gentiana scabra var. buergeri Extract on Toxoplasmastic Activity of Macrophages
Sung-Gu Kang,Yong-Suk Ryang,Insik Kim 대한의생명과학회 2003 Biomedical Science Letters Vol.9 No.2
Gentiana scabra var. buergeri (G. scabra) is a herb known to have therapeutic effect in infection diseases. We studied cellular activation and antitoxoplasmosis in macrophages after G. scabra stimulation. Macrophage activation was detected by nitrite production. Macrophages were treated with G. scabra extracted with water or methanol. Maximal nitrite production was detected in macrophages after stimulation of G. scabra extract 0.1 ㎎/㎖. Maximal nitrite concentration was 23.22 0.003 uM/L in macrophages after water extract of G. scabra and was 24.07 1.41 uM/L after methanol extract of G. scabra. Effect of G. scabra in the phagocytic capacity of macrophages was monitored by using PI (percentage of macrophage infected by T. gondiii method. The minimum PI (42.5 2.31) was detected in macrophages treated by water extract of G. scabra 0.1 ㎎/㎖ before infection of T. gondii. We also examined toxoplasmastatic capacity of macrophage using FI (fold increase) method. The minimum FI (4.46 1.16) was shown in macrophages after water extract of G. scabra 0.1 ㎎/㎖ pretreatment before infection. Under electron microscope, proliferation of T. gondii was inhibited by extract of G. scabra treatment in macrophages and the mitochondrion and lysosomal vacuoles within cells were increased. Taken together, G. scabra extract activates macrophages and induces toxopalsmastatic activity after T. gondii infection. It is suggested that G. scabra may be used as a therapeutic drug against toxoplasmosis.
Antiparasitic Effects of a Herb Extract from Gentiana scabra var buergeri on Trichomonas vaginalis
Ryang, Yong-Suk,Im, Jee-Aee,Kim, Insik,Cho, Yoon-Kyung,Sung, Ho-Joong,Park, Ju-Youn,MIn, Duk-Young,Ha, Ji-Yong 대한의생명과학회 2001 Journal of biomedical laboratory sciences Vol.7 No.2
We studied antitrichomoniasis with the extract of Gentiana scabra var buergeri, which may be effective in treating infectious diseases. The growth inhibition against T. vaginalis became optimal when the extract concentration was 0.7 mg/ml and the cells were seeded at a density of 3×105 per well. After incubation for 12,24,36, and 48 hrs, respectively, the number of cells were each 5×105, 1×105, 1×105, and none, respectively. Under the electron microscope, the experimental group showed that the nucleus, karyosomes, and chromatin were weaker than those in the control group. After incubating for 3 hrs, the cells were destroyed completely, and only a remnant remained. The hydrogenosomes disappeared almost. The vacuoles and autophagic vacuoles increased. The cells became regressive from.
Ryang Yong-Suk,Cho Yoon-Kyung,IM Jee-Aee,Lee Kyu-Je,Kim Insik,Sung Ho-Joong 대한의생명과학회 2004 Biomedical Science Letters Vol.10 No.3
This experiment was performed to examine the in vitro and in vivo affects of the two different haplotype strains of mice, BALB/c and C3H/HeN infected with Echinostoma hortense, and the manifestation of the profiles of cytokine in the splenocytes. In the in vitro experiment, the two mice's splenocytes were divided and stimulated with antigen of crude extracts and the antigen of excretory and secretory products of an adult warm and the manifestation of cytokine mRNA was verified with RT-PCR. As a result, the two different strains of mice both strongly manifested the Th2 cytokine rather than the Thl cytokine and in the case of the Th2 cytokine, the BALB/c mice manifested more strongly than the C3H/HeN mice. In the experiment using the ELISA method, the protem cytokine manifestation had the same result as the mRNA experiment. In the in vivo experiment, the mice was infected via oral route with the metacercaria of the Echinostoma hortense and the manifestation of cytokine was verified by RT-PCR and ELISA and the results were the same as the in vitro experiment. Therefore, in the two strains of BALB/c and C3H/HeN, the C3H/HeN showed a higher susceptivity to the Echinostoma hortense.
Resuscitation Fluid Use in a Single Surgical Intensive Care Unit
Yong Dae Lee,Jeong-Am Ryu,Dae-Sang Lee,Jinkyeong Park,Joongbum Cho,Chi Ryang Chung,Yang Hyun Cho,Jeong Hoon Yang,Gee Young Suh,Chi-Min Park 대한외상중환자외과학회 2020 Journal of Acute Care Surgery Vol.10 No.1
Purpose: The aim of this study was to analyze the temporal change of resuscitation fluid use based on all fluids administered in a surgical intensive care unit (ICU). Methods: The administration of resuscitation fluid to all patients admitted to a surgical ICU of a tertiary referral hospital was investigated from 2008 to 2015. The types and volumes of fluid, and laboratory data taken within 7 days after ICU admission were evaluated. Resuscitation fluids were defined as fluids infused according to stat orders, rather than routine orders. Results: There were a total of 8,885 admissions to the ICU for 7,886 patients. The volumetric proportion of crystalloid to total resuscitation fluids increased significantly over the study period (p < 0.001; 79.6% in 2008; 93.7% in 2015). Although the proportion of 0.9% saline to crystalloids decreased, that of balanced solutions increased (p < 0.001; 29.5% in 2008; 55.6% in 2015). The use of colloids decreased from 20.4% in 2008, to 6.3% in 2015 (p < 0.001). Proportions calculated using the number of individual fluids administered revealed trends similar to those calculated using volumetric data. The amount of infused 0.9% saline was weakly correlated with the lowest blood pH and the highest serum chloride levels (ρ = -0.26 and 0.19, respectively). Conclusion: Changes in the trends of fluid resuscitation practice were noted in a single surgical ICU over the 8-year study period. Crystalloid use increased owing to a rise in the utilization of balanced solutions with a downward trend in colloid use.
Antiparasitic Effects of a Herb Extract from Gentiana scabra var buergeri on Trichomonas vaginalis
Yong-Suk Ryang,Jee-Aee Im,Insik Kim,Yoon-Kyung Cho,Ho-Joong Sung,Ju-Youn Park,Duk-Young Min,Ji-Yong Ha 대한의생명과학회 2001 Biomedical Science Letters Vol.7 No.2
We studied antitrichomoniasis with the extract of Gentiana scabra var buergeri, which may be effective in treating infectious diseases. The growth inhibition against T. vaginalis became optimal when the extract concentration was 0.7㎎/㎖ and the cells were seeded at a density of 3×10? per well. After incubation for 12, 24, 36, and 48 hrs, respectively, the number of cells were each 5×10?, 1×10?, 1×10?, and none, respectively. Under the electron microscope, the experimental group showed that the nucleus, karyosomes, and chromatin were weaker than those in the control group. After incubating for 3 hrs, the cells were destroyed completely, and only a remnant remained. The hydrogenosomes disappeared almost. The vacuoles and autophagic vacuoles increased. The cells became regressive form.