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      • KCI등재

        Distribution and Pathogenicity of Fusarium Species Associated with Soybean Root Rot in Northeast China

        Yingying Liu,Xuena Wei,Feng Chang,Na Yu,Changhong Guo,Hongsheng Cai 한국식물병리학회 2023 Plant Pathology Journal Vol.39 No.6

        Fusarium root rot is an increasingly severe problem in soybean cultivation. Although several Fusarium species have been reported to infect soybean roots in Heilongjiang province, their frequency and aggressiveness have not been systematically quantified in the region. This study aimed to investigate the diversity and distribution of Fusarium species that cause soybean root rot in Heilongjiang province over two years. A total of 485 isolates belonging to nine Fusarium species were identified, with F. oxysporum and F. solani being the most prevalent. Pot experiments were conducted to examine the relative aggressiveness of different Fusarium species on soybean roots, revealing that F. oxysporum and F. solani were the most aggressive pathogens, causing the most severe root rot symptoms. The study also assessed the susceptibility of different soybean cultivars to Fusarium root rot caused by F. oxysporum and F. solani. The results indicated that the soybean cultivar DN51 exhibited the most resistance to both pathogens, indicating that it may possess genetic traits that make it less susceptible to Fusarium root rot. These findings provide valuable insights into the diversity and distribution of Fusarium species that cause soybean root rot and could facilitate the development of effective management strategies for this disease.

      • KCI등재

        Screening and Identification of ClpE Interaction Proteins in Streptococcus pneumoniae by a Bacterial Two-Hybrid System and Co-immunoprecipitation

        WenJuan Yan,YingYing Cai,Qun Zhang,YuSi Liu,WenChun Xu,YiBing Yin,YuJuan He,Hong Wang,XueMei Zhang 한국미생물학회 2013 The journal of microbiology Vol.51 No.4

        Hsp100/Clp proteins have crucial functions in the protein quality control, stress tolerance, and virulence of many pathogenic bacteria. ClpE is an important virulence factor involved in adherence and invasion in Streptococcus pneumoniae. To explore the underlying mechanism, we screened ClpE interaction proteins using a bacterial two-hybrid system and co-immunoprecipitation. We used ClpE as bait and constructed the pBT-ClpE bait plasmid for two-hybrid screening. Then, we constructed ClpE::GFP fusion for co-immunoprecipitation screening using anti-GFP monoclonal antibody. We obtained eight potential ClpE interaction proteins,including carbamoyl-phosphate synthase, pyruvate oxidase (SpxB), phosphoenolpyruvate-protein phosphotransferase,aminopeptidase N (pepN), L-lactate dehydrogenase, ribosomal protein S4, sensor histidine kinase (SPD_2019), and FtsW (a cell division protein). FtsW, SpxB, pepN, and SPD_2019 were confirmed to interact with ClpE using Bacterial Two-hybrid or Co-immunoprecipitation. Morphologic observations found that ΔclpE strain existed in abnormal division. β-Galactosidase activity assay suggested that ClpE contributed to the degradation of FtsW. Furthermore, FtsW could be induced by heat shock. The results suggested that ClpE might affect cell division by regulating the level of FtsW. These data may provide new insights in studying the role of ClpE in S. pneumoniae.

      • Incorporating Reduced Graphene Oxide with Magnetic Nanoparticles to Boost Microwave Absorption Properties

        Lei Sun,Yingying Cai,Ick Soo Kim,Qilong Sun 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2

        The rapid development of electronic technology and intensive use of communication devices have caused the inevitable leakage of electromagnetic waves, leading to serious electromagnetic pollution. To drive this problem, we fabricated Fe₃O₄ decorated reduced graphene oxide (Fe₃O₄-rGO) nanocomposites for electromagnetic wave absorption by an in situ reduction process. The as-prepared Fe₃O₄-rGO showed a typical sandwich-like structure, Fe₃O₄ nanoparticles were flaky in shape and strongly attached on rGO by C-O-Fe bond. The hysteresis loops revealed the super-paramagnetic behavior of the Fe₃O₄-rGO nanocomposites at room temperature, which has a considerable contribution to electromagnetic wave absorption. When testing the electromagnetic wave absorption properties, the Fe₃O₄-rGO nanocomposites exhibited multiple dielectric relaxations and magnetic resonance, which can be ascribed to the well-designed structure, the strong chemical bond between Fe₃O₄ and rGO, as well as super-paramagnetic characteristics. As a result, the maximum reflection loss reached -49.53 dB at 6.32 GHz for a thickness of 3.4 mm while the highest effective absorption bandwidth was 2.96 GHz.

      • KCI등재

        Mean-square Exponential Stability of Impulsive Stochastic Time-delay Systems with Delayed Impulse Effects

        Lijun Gao,Dandan Wang,Yingying Cai 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.3

        This paper is concerned with the mean-square exponential stability problem for a class of impulsivestochastic systems with delayed impulses. The delays exhibit in both continuous subsystem and discrete subsystem. By constructing piecewise time-varying Lyapunov functions and Razumikhin technique, sufficient conditions arederived which guarantee the mean-square exponential stability for impulsive stochastic delay system. It is shownthat the obtained stability conditions depend both on the lower bound and the upper bound of impulsive intervals,and the stability of system is robust with regard to sufficiently small impulse input delays. Finally, two examplesare proposed to verify the efficiency of the proposed results.

      • KCI등재

        Genetic individualization of sable (Martes zibellina L. 1758) using microsatellites

        Bo Li,DanWu,Yingying Cai,Monakhov Vladimir G,Wei Zhang,Yanchun Xu 한국통합생물학회 2018 Animal cells and systems Vol.22 No.4

        Genetic individualization based on non-invasive sampling is crucial for estimating the numbers of individuals in endangered mammalian populations. In sable (Martes zibellina)-poaching cases, identifying the number of animals involved is critical for determining the penalty. In addition, investigating animal numbers for wild sable populations requires genetic individualization when collecting several samples in neighboring regions. Microsatellites have been demonstrated to be reliable markers for individual identification. Thirty-three microsatellite loci derived from Mustelidae were selected to develop a genetic individualization method for sable. Three reference populations containing 54 unrelated sables were used to calculate allele number, allelic frequencies, and the polymorphic information content of each locus. The data were subsequently used to assess the validity of a combination of twelve loci for sable individualization. We defined twelve polymorphic loci that were easy to be amplified and genotyped. Four significant deviations from Hardy-Weinberg equilibrium were observed among the 12 loci in the three populations. The match probability of an individual from the reference populations with a random individual based on the 12 loci was 1.37 × 10−13. Using the combination of the twelve loci provides sufficient power to individualize sables considering the levels of microsatellite polymorphism observed. These loci were successfully applied to a case of sable poaching and provided valid evidence to determine the penalty. The genetic individualization of sable based on these loci might also be useful to investigate the numbers of animals in wild populations.

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