Variety of Recombinant Inbred Line Population and Creation of Germplasm with High Yield in Sorghum-Sudan Grass

Xiaoping Lu,Jinfeng Yun,Shuyan Wang 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

Sorghum-Sudan grass which is an annual forage grass in Gramineae have been bred for biomass promotion. The recombinant inbred line (RIL) populations were derived from the F2:3 genetic mapping population of Sorghum 314A×zongke Sudan grass. The breeding selection based on agronomic characters and DNA molecular marker statistical analysis was carried among the progenies of the RIL populations, and 9 recombinant breeding progenies were determined as heterobeltiosis with high yield. According to DNA diversity analysis of simple sequence of repeats(SSR) marker, the genetic varieties were abundant among the families, and the recombinant breeds of high yield were distributed among the different RIL populations.2 family lines, which have similar agronomic characters and yield, and whose genetic distance nearly equal to zero, showed difference on arid tolerance. 4 breeding lines were selected on agronomic characters and resistance in the field trials, and 2 breed lines were qualified for the regional testing.

Ecological Fitness of Brown Planthopper Nilaparvata lugens and its Predator Cyrtorhinus Lividipenni son Rice Plants with Different Nitrogen Regimes

Zhongxian Lu,K. L Heong,Xiaoping Yu 한국응용곤충학회 2005 한국응용곤충학회 학술대회논문집 Vol.2005 No.05

The Biotypes, Wing-forms and the Immigration of Brown Planthopper, Nilaparvata lugens Stal, in Zhejiang Province, China

Yu, Xiaoping,Lu, Zhongxian,Wu, Guorui,Tao, Linyong,Chen, Jianming,Zheng, Xusong,Xu, Hongxing Korean Society of Applied Entomology 2001 Journal of Asia-Pacific Entomology Vol.4 No.2

The virulence change (biotype), wing form development and immigration of brown planthopper (BPH) Nilaparvata lugens Stal, population in Zhejiang province was studied as compared to these BPH populations collected from Guangxi and Yunnan province, China and Philippines. The results indicate that the Zhejiang population could successfully infest variety IR26 and Mudgo after 1989, and ASD7 and IR36 after 1998, showing the characters of BPH biotype 2 and biotypes 3. The BPH tropical populations such as Guangxi population, however, adapted the resistant varieties IR26, Mudgo and ASD7 earlier. In 1997, the nymphal survival indices of Guangxi and Yunnan populations on Rathu Heenati (Bph3) were up to 70.9 and 66.7 respectively, higher than Zhejiang population. The Zhejiang populations show low brachupterous rates and have a negative correlation with nymphal density, belonging to the temperate type. The Philippine populations are almost brachypterous, however, the brachypterous rates of BPH males raise with the increase of nymphal density. The Guangxi population has a high brachypterous rate, being similar to the tropical type. On the other hand, tests showed that the lowest survival rate, longer duration of nymphs and the lightest weight of brachypterous female occurred in the $2^{nd}$ generation of BPH after continuously feeding on resistant variety. Physiologically, the highest activities of superoxide dismutase (SOD) and catalase (CAT) were detected in the second generation of the BPH feeding on resistant rice variety.

Investigation on characteristics of pulverized coal dense-phase pneumatic conveying under high pressure

Chen Xiaoping,Fan Chunlei,Liang Cai,Pu Wenhao,Lu Peng,Zhao Changsui 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.3

of dense-phase pneumatic conveying of pulverized coal were carried out in a test facility witha conveying pressure up to 4 MPa. The influence of fluidization nitrogen flow rate, the flow rate of supplementarysolid mass flow rate was investigated. Test results indicate that with the increase in fluidization nitrogen flow rate, thesolid mass flow rate increases, and the solids to gas ratio increases at first and then declines. When the fluidization ofpulverized coal in the sending vessel becomes intensive, with the increase in supplementary nitrogen flow rate, thesolids to gas ratio declines and the solid mas flow rate increases. And the solid mass flow rate increases linearly withthe increase in presure diference between two hoppers. The experimental results provide a database for the design

Flow characteristics and dynamic behavior of dense-phase pneumatic conveying of pulverized coal with variable moisture content at high pressure

Liang Cai,Chen Xiaoping,Zhao Changsui,Pu Wenhao,Lu Peng,Fan Chunlei 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.3

Experiments of dense-phase pneumatic conveying of pulverized coal using nitrogen were performed in an experimental test facility with the conveying pressure up to 4MPa and the solid-gas ratio up to 500 kg/㎥. The influences of the total conveying differential pressure, the moisture content, the superficial velocity and the pressure on the mass flow rate and the solid-gas ratio were investigated. Shannon entropy analysis of pressure fluctuation time series was developed to reveal the flow characteristics. Based on the distribution of the Shannon entropy in the different conditions, the flow stability and the evolutional tendency of Shannon entropy in different regimes and the regime transition processes were obtained. The results indicate that the solid gas ratio and Shannon entropy rise with increase in the total conveying differential pressure. A phase diagram and Shannon entropy reveal preferable regularity with superficial velocity. Shannon entropy is different for the different flow regimes, and it can be used to identify the flow regimes. As the moisture content increases, the mass flow rate, the pressure drop and Shannon entropy decrease. Shannon entropy rises with increase in pressure drop.

Acoustically aided coalescence of water droplets and dehydration of crude oil emulsion

Wei Xie,Rui Li,Xiaoping Lu,Pingfang Han,Shanlong Gu 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.4

We studied the use of acoustics for coalescence of water droplets and dehydration of crude oil emulsion. Experimental studies were conducted using acoustic standing waves in a resonant cavity to trap water droplets andenhance oil separation. The focus was on the effect of ultrasound irradiation on crude oil emulsion properties, such asviscosity, water drop radius, shear strength of oil-water interfacial films, and flocculation size of asphaltene. These propertiesare important to the coalescence of water drops in water-oil (W/O) emulsion in the process of oil separation withultrasound. Ultrasound irradiation is able to decrease the emulsion stability, which provides a new insight into theacoustics-aided demulsification mechanism. It can be considered as a supplement of traditional acoustics-aided demulsificationmechanism (ultrasound-induced motion of water droplets). Furthermore, the effects of ultrasonic parameterssuch as the type of ultrasonic field, irradiation time, frequency, and acoustic intensity on dewatering the W/O emulsionare discussed. These results provide guidance for setting the optimum conditions for the separation of W/O emulsionwith ultrasound. Under the optimum conditions, water content in crude oil emulsion can be decreased from 40%to 3.8%, which satisfies the requirement of dehydration for refinery.

NlCDK1 gene is required for the ovarian development and survival of the brown planthopper, Nilaparvata lugens Stål, as revealed by RNA interference

Peiying Hao,Yan Ma,Yalin Feng,Chaofeng Lu,Xiaoping Yu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

CDK1 is a kind of cyclin-dependent kinases (CDKs) involved in diverse biological processes besides cell cycleregulation in various species. However, little is known about CDK1 in Nilaparvata lugens Stål (brown planthopper,BPH). In this study, the full-length cDNA of NlCDK1, a CDK1 gene in BPH, was obtained using rapidamplificationof cDNA ends (RACE) technique. RT-qPCR revealed that the expression of NlCDK1 was relativelylow and stable at nymph stages from 1st star to 5th star, but increased to higher levels in gravid females 3 day to5 day post-eclosion. Feeding-based RNA interference (RNAi) with 0.5 μg/μL dsNlCDK1 achieved an efficientknockdown of target gene expression in females after being fed with dsRNA for 6 days, and the mRNA level ofNlCDK1 decreased to 19.9% in comparison with that of the dsGFP control. As a result, RNA inerference withdsNlCDK1 caused a significant decrease of the BPH survival rate to 28.0% of that for the control. RNAi of NlCDK1also suppressed the ovarian development and significantly affected the fecundity of the females, leading to a70.2% reduction to the number of the offspring. These data suggest that NlCDK1 is required for the ovariandevelopment and survival of the brown planthopper.

Identification of drug target candidates of the swine pathogen Actinobacillus pleuropneumoniae by construction of protein–protein interaction network

Siqi Li,Zhipeng Su,Chengjun Zhang,Zhuofei Xu,Xiaoping Chang,Jiawen Zhu,Ran Xiao,Lu Li,Rui Zhou 한국유전학회 2018 Genes & Genomics Vol.40 No.8

Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae has led to severe economic losses in the pig industry worldwide. A. pleuropneumoniae displays various levels of antimicrobial resistance, leading to the dire need to identify new drug targets. Protein–protein interaction (PPI) network can aid the identification of drug targets by discovering essential proteins during the life of bacteria. The aim of this study is to identify drug target candidates of A. pleuropneumoniae from essential proteins in PPI network. The homologous protein mapping method (HPM) was utilized to construct A. pleuropneumoniae PPI network. Afterwards, the subnetwork centered with H-NS was selected to verify the PPI network using bacterial two-hybrid assays. Drug target candidates were identified from the hub proteins by analyzing the topology of the network using interaction degree and homologous comparison with the pig proteome. An A. pleuropneumoniae PPI network containing 2737 non-redundant interaction pairs among 533 proteins was constructed. These proteins were distributed in 21 COG functional categories and 28 KEGG metabolic pathways. The A. pleuropneumoniae PPI network was scale free and the similar topological tendencies were found when compared with other bacteria PPI network. Furthermore, 56.3% of the H-NS subnetwork interactions were validated. 57 highly connected proteins (hub proteins) were identified from the A. pleuropneumoniae PPI network. Finally, 9 potential drug targets were identified from the hub proteins, with no homologs in swine. This study provides drug target candidates, which are promising for further investigations to explore lead compounds against A. pleuropneumoniae.