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Glycine alleviated diquat-induced hepatic injury via inhibiting ferroptosis in weaned piglets
Hua Hongwei,Xu Xiao,Tian Wei,Li Pei,Zhu Huiling,Wang Wenjun,Liu Yulan,Xiao Kan 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.6
Objective: The beneficial effects of glycine were tested in piglets with diquat-induced hepatic injury. Methods: Thirty-two piglets were assigned by a 2×2 factorial experimental design including glycine supplementation and diquat challenge. After 3 weeks of feeding with a basic diet or a 1% glycine supplemented diet, piglets were challenged with diquat or saline. After 1 week later, the piglets were slaughtered and samples were collected. Results: Our results indicated that glycine alleviated diquat induced morphological hepatic injury, decreased the activities of plasma alanine aminotransferase, aspartate aminotransferase and glutamyl transpeptidase in the piglets under diquat challenge, and increased total antioxidant capacity and antioxidative enzyme activity significantly. Adding glycine enhanced the concentrations of hepatic adenosine triphosphate and adenosine diphosphate. Transmission electron microscope observation showed that diquat induced clear hepatocytes ferroptosis and its effect could be alleviated by glycine to a certain degree. Moreover, glycine significantly affected mRNA and protein expression of ferroptosis-related signals in the liver. Conclusion: These results demonstrated that glycine attenuated liver damage via inhibiting ferroptosis. Objective: The beneficial effects of glycine were tested in piglets with diquat-induced hepatic injury.Methods: Thirty-two piglets were assigned by a 2×2 factorial experimental design including glycine supplementation and diquat challenge. After 3 weeks of feeding with a basic diet or a 1% glycine supplemented diet, piglets were challenged with diquat or saline. After 1 week later, the piglets were slaughtered and samples were collected.Results: Our results indicated that glycine alleviated diquat induced morphological hepatic injury, decreased the activities of plasma alanine aminotransferase, aspartate aminotransferase and glutamyl transpeptidase in the piglets under diquat challenge, and increased total antioxidant capacity and antioxidative enzyme activity significantly. Adding glycine enhanced the concentrations of hepatic adenosine triphosphate and adenosine diphosphate. Transmission electron microscope observation showed that diquat induced clear hepatocytes ferroptosis and its effect could be alleviated by glycine to a certain degree. Moreover, glycine significantly affected mRNA and protein expression of ferroptosis-related signals in the liver.Conclusion: These results demonstrated that glycine attenuated liver damage via inhibiting ferroptosis.
Curcumin Inhibits Expression of Inhibitor of DNA Binding 1 in PC3 Cells and Xenografts
Yu, Xiao-Ling,Jing, Tao,Zhao, Hui,Li, Pei-Jie,Xu, Wen-Hua,Shang, Fang-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3
Inhibitor of DNA binding 1 (Id1) plays an important role in genesis and metastatic progression of prostate cancer. We previously reported that down regulation of Id1 by small interfering RNA could inhibit the proliferation of PC3 cells and growth of its xenografted tumors. Curcumin, the active ingredient of turmeric, has shown anti-cancer properties via modulation of a number of different molecular regulators. Here we investigated whether Id1 might be involved in the anti-cancer effects of curcumin in vivo and in vitro. We firstly confirmed that curcumin inhibited cell viability in a dose-dependent fashion, and induced apoptosis in PC3 cells, associated with significant decrease in the mRNA and protein expression of Id1. Similar effects of curcumin were observed in tumors of the PC3 xenografted mouse model with introperitoneal injection of curcumin once a day for one month. Tumor growth in mice was obviously suppressed by curcumin during the period of 24 to 30 days. Both mRNA and protein levels of Id1 were significantly down-regulated in xenografted tumors. Our findings point to a novel molecular pathway for curcumin anti-cancer effects. Curcumin may be used as an Id1 inhibitor to modulate Id1 expression.
Peptidoglycans Promotes Human Leukemic THP-1 Cell Apoptosis and Differentiation
Wang, Di,Xiao, Pei-Ling,Duan, Hua-Xin,Zhou, Ming,Liu, Jin,Li, Wei,Luo, Ke-Lin,Chen, Jian-Jun,Hu, Jin-Yue Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12
The innate immune system coordinates the inflammatory response to pathogens. To do so, its cells must discriminate self from non-self utilizing receptors that identify molecules synthesized exclusively by microbes. Toll-like receptors have a crucial role in the detection of microbial infection in mammals and insects. In mammals, they have evolved to recognize conserved products unique to microbial metabolism. These include lipopolysaccharide (LPS), lipotechoic acids, and peptidoglycans (PGN). We show here that TLRs, including TLR2, are expressed on the THP-1 human leukemia cell line. Activation of TLR2 signaling in THP-1 by PGN induces the synthesis of various soluble factors and proteins including interleukin-$1{\beta}$, interleukin-8 and TNF-${\alpha}$ and apoptosis of THP-1 with PGN dose and time dependence. Moreover, in this study we show that PGN induces apoptosis of THP-1 cells in a TNF-${\alpha}$-dependent manner. These findings indicate that TLR2 signaling results in a cascade leading to tumor apoptosis and differentiation, which may suggest new clinical prospects using TLR2 agonists as cytotoxic agents in certain cancers.
Ping Wei,Pei Xu,Xiao-Ting Wang,Wen-Yong Lou,Min-Hua Zong 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.2
The asymmetric reduction of ethyl acetoacetate (EAA) to ethyl (R)-3-hydroxybutyrate [(R)-EHB] using immobilized Acetobacter sp. CCTCC M209061 cells was successfully conducted in a hydrophilic ionic liquid (IL)- containing system. The best one of all the tested watermiscible ILs was 1-(hydroxyethyl)-3-methylimidazolium hydrochloride (C2OHMIM·Cl). In C2OHMIM·Cl-aqueous buffer hybrid system, it was found that the optimal IL concentration, substrate and co-substrate concentration, reaction temperature, buffer pH and shaking rate were 0.5mol/L, 45 mmol/L, 80 mmol/L, 35oC, pH 5.5 and 200 rpm, respectively. Under the optimized reaction conditions, the initial reaction rate, the yield and the product e.e. reached 4.90 μmol/min, 95.3 and > 99.0%, respectively, which were much higher than the corresponding values reported previously. The efficient biocatalytic process mediated by the immobilized cells was feasible on 500 mL preparative scale, and the biocatalysts showed good operational stability and could be recycled for at least 10 batches.
PREPARATION OF NANO-TATB BY SEMIBATCH REACTION CRYSTALLIZATION
XUE-RONG TAN,XIAO-HUI DUAN,CHONG-HUA PEI,HONG-LIN XU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.5
The 1,3,5-triamino-2,4,6-trinitrobenzene (TATB) nanoparticles were prepared by using semibatch reaction crystallization method, and the influencing factors in close relationship with the grain size and crystal morphology control, such as the concentration of reaction system and categories of surfactants, were studied in this paper. The synthesized nano-TATB particles had been characterized by SEM, XRD, thermo gravimetric/differential scanning calorimetric (TG/DSC) and N2 physisorption. The grain size of TATB particles using nonionic surfactant as the additive ranged from 30 nm to 65 nm with a shape of spheres or ellipsoids. The broadening of the peaks and the weakening of the strength for nano-TATB were observed by XRD analysis. The corrected average particle size of nano-TATB was calculated using the Debye–Scherrer equation and the range was from 18 nm to 50 nm. TG and DSC curves revealed that thermal decomposition of nano-TATB occurred in the range of 361.5°C–385.0°C and its peak temperature was 373.7°C with a decrease of approximately 7°C compared with original TATB. Furthermore, the specific surface area (21.54 m2/g) of nano-TATB was calculated by BET method using N2 physisorption (at 77°C).