Study on maximizing catalytic performance of cobalt(II) 5,10,15, 20-tetrakis(4-pyridyl)porphyrin for cyclohexane oxidation

Xian-Fei Huang,Guang-Ping Yuan,Guan Huang,Su-Juan Wei 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.77 No.-

Aiming at the most enhancing the catalytic performance of simple and cheap cobalt(II) 5,10,15,20-tetrakis(4-pyridyl)porphyrin (Co TPyP), guided by the nowadays-advanced various technologies, Co TPyP wasanchored onto nanoporous bar with thiol-functionalized silica (npb-SiO2-SH). The resulting npb-SiO2-SCoTPyP catalyst was characterized using various techniques, determining its structure. The total surfacearea ratios (TSAR) of the immobilized cobalt porphyrin to the npb-SiO2-SH, and the catalytic cyclohexaneoxidation reaction conditions were at the same time optimized via Response Surface Methodology(RSM). Compared with those obtained from traditional optimizing method (TOM), on average, RSM couldenhance as high as more than 60% catalyst turnover frequency (TOF) and yields of cyclohexanone andcyclohexanol (KA oil). The anchored 0.05 mg Co TPyP could catalyze the oxidation of 200 mL ofcyclohexane, under not using any solvents and additives, offering 2.5106 1/h of TOF, and 19% of yields. The npb-SiO2-S (20%-0.0438)-Co TPyP catalyst showed better reusability, excellent catalytic

Anti-inflammatory and Anticancer Activities of Ethanol Extract of Pendulous Monkshood Root in vitro

Huang, Xian-Ju,Ren, Wei,Li, Jun,Chen, Lv-Yi,Mei, Zhi-Nan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Aim: Pendulous monkshood root is traditionally used for the treatment of several inflammatory pathologies such as rheumatisms, wounds, pain and tumors in China. In this study, the anti-inflammatory and anticancer activities and the mechanism of crude ethanol extract of pendulous monkshood root (EPMR) were evaluated and investigated in vitro. Materials and Methods: The cytotoxic effects of EPMR on different tumor cell lines were determined by the MTT method. Cell apoptosis and cell nucleus morphology were assessed by Hoechst 33258 staining. Moreover, nitric oxide (NO) levels and intracellular oxidative stress in peritoneal macrophages were determined to further elucidate mechanisms of action. Results: The data showed that EPMR could produce significant dose-dependent toxicity on three kinds of tumor cells. Furthermore, EPMR displayed obvious anti-inflammatory effects on LPS-induced mouse peritoneal macrophages at the dosage of 4 - 200 ${\mu}g/mL$. The results demonstrated the therapeutic potential of Pendulous Monkshood Root on cancer and inflammatory diseases. Conclusion: Our results indicate that EPMR has anti-inflammatory and anticancer properties, suggesting that pendulous monkshood root may be a useful anti-tumor and anti-inflammatory reagent in the clinic.

International faculty in China: case studies of four leading universities in Shanghai

Xian Wu,Futao HUANG 서울대학교 교육연구소 2018 Asia Pacific Education Review Vol.19 No.2

To attract and hire high-level international faculty to leading universities is an important task in building world-class universities in China. However, there is much left to explore and to understand about international faculty in China. Based on a brief review of China’s higher education system and international faculty, findings from A National Survey of International Faculty at Chinese Universities and Colleges (conducted in 2017), and case studies of four leading universities in Shanghai, this study explores the main characteristics and motivations of international faculty in China by discipline, age, gender, and so forth. More specially, it explores the individual, educational, and professional characteristics of international faculty at Chinese colleges and universities, how these characteristics vary among different groups of international faculty, and why they came to Chinese leading universities?

Disruption of the Myostatin Gene in Porcine Primary Fibroblasts and Embryos Using Zinc-Finger Nucleases

Xian-Ju Huang,Hong-Xiao Zhang,Huili Wang,Kai Xiong,Ling Qin,Honglin Liu 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.4

Myostatin represses muscle growth by negatively regulating the number and size of muscle fibers. Myostatin loss-of-function can result in the double-muscling phenotype and increased muscle mass. Thus, knockout of myostatin gene could improve the quality of meat from mammals. In the present study, zinc finger nucleases, a useful tool for generating gene knockout animals, were designed to target exon 1 of the myostatin gene. The designed ZFNs were introduced into porcine primary fibroblasts and early implantation embryos via electroporation and microinjection, respectively. Mutations around the ZFNs target site were detected in both primary fibroblasts and blastocysts. The proportion of mutant fibroblast cells and blastocyst was 4.81% and 5.31%, respectively. Thus, ZFNs can be used to knockout myostatin in porcine primary fibroblasts and early implantation embryos.

ALMOST RIGIDITY OF CONVEX HYPERSURFACES VIA THE EXTINCTION TIME OF MEAN CURVATURE FLOW

Huang, Xian-Tao Korean Mathematical Society 2021 대한수학회보 Vol.58 No.4

We prove that if a compact convex hypersurface of ℝⁿ⁺¹ has almost maximal extinction time when it is evolved by the mean curvature flow, then it must be nearly round in the C⁰-norm.

Transgenic Rice Overexperessing a Tomato Mitochondrial Phosphate Transporter, SlMPT3;1, Promotes Phosphate Uptake and Increases Grain Yield

Xian-guo Cheng,Guo-hong Yu,Sheng-cai Huang,Rui He,Ying-Zhang Li 한국식물학회 2018 Journal of Plant Biology Vol.61 No.6

Mitochondrial phosphate transporter plays animportant regulatory role in promoting the uptake andtransport of phosphate in plants. In this study, the SlMPT3;1gene, a member of mitochondrial phosphate transporterfamily in tomato, was isolated and transformed into the riceOryza sativa L. ssp. japonica cultivar Kitaake. The SlMPT3;1 islocalized to the mitochondrial membrane and functions incompensating the phosphate uptake in yeast MB192 mutantthat is defective in phosphate transport under Pi deficiency. RT-qPCR showed that the SlMPT3;1 is expressed in all oftomato tissues, but highly accumulated in the young leavesand stems under Pi deficiency. The data demonstrated that atleast two copies of the SlMPT3;1 gene are inserted into therice genome, and the transcripts of the SlMPT3;1 mRNA arehighly accumulated in the roots of the transgenic rice. Theoverexpression of the SlMPT3;1 gene not only promotesphosphate uptake by the roots, but also increases thetranslocation of phosphate from the roots to the shoots in thetransgenic rice. The transgenic rice accumulated morechlorophyll and soluble sugar in the shoots than the wildtype under Pi deficiency. Microassay sequencing showedthat the differentially expressed genes in the transgenic riceare mainly involved in the regulations of biological processand molecular function under Pi deficiency. Further RTqPCRanalyses revealed that the differentially expressedgenes, which are involved in the regulations of the biologicalprocess, cell component, and molecular function, are upregulatedunder Pi deficiency, and exhibit similar expressiontrends to the relative expression folds of these partial differentiallyexpressed genes in the transcriptomic analyses. This studysuggests that the overexpression of the SlMPT3;1 gene promotedthe uptake and transport of phosphate in rice, thus leading to anenhanced increase in tiller number and effective panicle of perplant, and increasing grain yield under Pi deficiency.

Novel genetic variants of sine oculis homeobox homolog 3 gene are associated with body weight and average daily gain in Bos taurus

Xian-Yong Lan,Chu-Zhao Lei,Yong-Tao Huai,Chuan-Ying Pan,Jing Wang,Yong-Zhen Huang,Hong Chen 한국유전학회 2011 Genes & Genomics Vol.33 No.6

Sine oculis homeobox homolog 3 (Six3) gene is responsible for normal mammalian pituitary development, and its genetic variations or deficiency will cause hypopituitarism, suggesting that this gene is a potential candidate gene for studying association with growth traits in animals. Herein, genetic variants within bovine Six3 gene was screened in 1031 individuals from four Chinese indigenous cattle breeds. Two novel polymorphisms (NC_007309:g.2515G>A and NC_007309:g.2607T>C) locating at positions nt1707 and nt1799 of intron 1 in bovine Six3gene, were found, and could be genotyped by TaqI ACRS PCR-RFLP and Alw26I PCR-RFLP, respectively. The frequencies of allele “A” of TaqI locus varied from 0.004 to 0.309,as well as the frequencies of allele “C” of Alw26I locus waved from 0.025 to 0.340. Association analysis revealed no significant association of TaqI locus with growth traits in Nanyang breed. However, significant relationships between Alw26I locus and body weight (BW) and average daily gain (ADG) in Nanyang breed was found (p<0.05). The individuals with genotype TC had greater body weight and average daily gain than those with genotype TT at 18 months old. Furthermore, based on combinated genotypes from these two loci, diplotypes was found to be associated with growth traits (p<0.05).The individuals with dihaplotype GG-TC had greater body weight and average daily gain at 18 month-old than those of other dihaplotypes. Therefore, the TaqI and Alw26I genetic variants of bovine Six3 gene were recommended as DNA markers related to growth traits through marker-assisted selection for genetics and breeding in cattle.

A Novel Two-Step Tensegrity Topology-Finding Method Based on Mixed Integer Programming and Nonlinear Programming

Xian Xu,Shaoxiong Huang,Tingting Shu,Yafeng Wang,Yao-Zhi Luo 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.4

A two-step topology-fi nding method based on mixed integer programming and nonlinear programming is proposed for tensegrity structures. In the fi rst step, feasible and symmetric strut connectivities are obtained through a ground structure method combined with mixed integer programming; in the second step, the cable connectivities are optimized through nonlinear programming to obtain a feasible tensegrity structure. The same ground structure used in the fi rst step is adopted in the second step, which is benefi cial to fi nd a more symmetric cable layout. The independent continuous mapping method is used in the second step to convert the 0–1 binary variables of cable connectivities to continuous variables to transform the combinatorial optimization problem into a nonlinear programming problem. The number of strut lengths is adopted as a control parameter and a symmetry objective function is proposed to generate a variety of regular and symmetric tensegrity structures. A multi-stage computation scheme is proposed to improve the computational effi ciency. Typical examples are carried out to validate the proposed method. The computational effi ciency of the method is benchmarked with existing methods fully based on mixed integer programming. Results demonstrate that the computational effi ciency of the proposed method is signifi cantly improved compared to the existing methods.

Disruption of the Myostatin Gene in Porcine Primary Fibroblasts and Embryos Using Zinc-Finger Nucleases

Huang, Xian-Ju,Zhang, Hong-Xiao,Wang, Huili,Xiong, Kai,Qin, Ling,Liu, Honglin Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.4

Myostatin represses muscle growth by negatively regulating the number and size of muscle fibers. Myostatin loss-of-function can result in the double-muscling phenotype and increased muscle mass. Thus, knockout of myostatin gene could improve the quality of meat from mammals. In the present study, zinc finger nucleases, a useful tool for generating gene knockout animals, were designed to target exon 1 of the myostatin gene. The designed ZFNs were introduced into porcine primary fibroblasts and early implantation embryos via electroporation and microinjection, respectively. Mutations around the ZFNs target site were detected in both primary fibroblasts and blastocysts. The proportion of mutant fibroblast cells and blastocyst was 4.81% and 5.31%, respectively. Thus, ZFNs can be used to knockout myostatin in porcine primary fibroblasts and early implantation embryos.

MicroRNA-21 Regulates the Invasion and Metastasis in Cholangiocarcinoma and May Be a Potential Biomarker for Cancer Prognosis

Huang, Qiang,Liu, Lei,Liu, Chen-Hai,You, Hao,Shao, Feng,Xie, Fang,Lin, Xian-Sheng,Hu, San-Yuan,Zhang, Chuan-Hai Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Background: MicroRNAs are noncoding RNA molecules that posttranscriptionally regulate gene expression. The aim of this study was to determine the role of microRNA-21 in cholangiocarcinomas and its relationship to cholangiocarcinoma RBE cell capacity for invasion and metastasis. Methods: MicroRNA-21 expression was investigated in 41 cases of cholangiocarcinoma samples by in situ hybridization and real-time PCR. Influence on cholangiocarcinoma cell line invasion and metastasis was analyzed with microRNA-21 transfected cells. In addition, regulation of reversion-inducing-cysteine-rich protein with kazal motifs (RECK) by microRNA-21 was elucidated to identify mechanisms. Results: In situ hybridization and real-time quantitative PCR results for patients with lymph node metastasis or perineural invasion showed significantly high expression of microRNA-21 (P<0.05). There was a dramatic decrease in cholangiocarcinoma cell line invasion and metastasis ability after microRNA-21 knockdown (P<0.05). However, overexpression significantly increased invasion and metastasis (P<0.05). Real-time PCR and Western-blot analysis showed that microRNA-21 could potentially inhibit RECK expression in RBE cells. Survival analysis showed that patients with higher expression levels of microRNA-21 more often had a poor prognosis (P<0.05). Conclusions: MicroRNA-21 may play an important role in cholangiocarcinoma invasion and metastasis, suggesting that MicroRNA-21 should be further evaluated as a biomarker for predicting cholangiocarcinoma prognosis.