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토끼 복부 내벽으로부터 분리된 경락으로 믿어지는 관조직
손준형 ( Joon Hyung Sohn ),윤진하 ( Jin-ha Yoon ),김영주 ( Young Joo Kim ),김민경 ( Min Kyung Kim ),김지화 ( Ji Hwa Kim ),권오현 ( Ohyun Kwon ),김현원 ( Hyun-won Kim ) 대한미용학회(구 대한미용과학회) 2016 대한미용학회지 Vol.12 No.4
Investigation of meridian line and acupoints as anatomical entities should be very important, as acupuncture and meridian massage for medical purpose in the hospital or for skin care in beauty shop are each based on the traditional meridian theory. We isolated tubular structures under the skin of rabbits where the traditional meridian lines are located. The characteristics of these tubular structures isolated from the abdominal wall matches those of Bonghan tubes and Bonghan tubules found by Bong Han Kim. The Bonghan theory established by Bong Han Kim suggests that meridian lines consist of Bonghan tubes and Bonghan tubules. We could observe the presence of large cells inside the Bonghan tubule and the presence of small granules referred to as Sanal in Bonghan theory inside the striae consisting of the Bonghan tubules. Present research suggests that there exist a third unique tubular structure where Sanals flow other than Bonghan tube and Bonghan tubules.
Distribution of Aquaporins (Water Channels) in the Rat Salivary Glands
정지연(Ji-Yeon Jung),한창룡(Chang-Ryoung Han),정연진(Yeon-Jin Jeong),오원만(Won-Mann Oh),김미원(Mi-Won Kim1),김선헌(Sun-Hun Kim),김옥준(Ok-Joon Kim),김현진(Huyn-Jin Kim),고정태(Jeong-Tae Koh),최홍란(Hong-Ran Choi),김원재(Won-Jae Kim) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.3
침샘은 하루 1.5 l의 침을 분비하며, 일반적으로 침샘에서 수분이동은 능동적 염분 이동에 따른 삼투현상에 의해 일어난다. 따라서 침샘에서 높은 수분투과성으로 인해 수분통로 단백인 aquaporin (AQP)들이 풍부하게 존재할 수 있다. 지금까지침샘에 4가지 형태의 AQP이 존재한다고 알려져 있지만 침샘에서 정확한 위치와 수분 이동에 대한 각 AQP들의 역할은 아직 확실치 않다. 본 연구는 300 g 정도의 흰쥐를 pentobarbital sodium (50 mg/kg, IP) 마취하에서 carbarchol (10 μg/kg)을 복강 내 투여한 후 AQP들의 역할과 분포를 면역조직화학방법으로 조사하였다. AQP1은 침샘의 미세혈관의 내피세포와 샘세포와 샘관 주위에 존재하는 근육상피세포에 존재하였다. AQP4는 침샘관의 마지막 부위인 배출관에 존재하였다. AQP5는 주로 장액세포의 바닥가쪽과 세포사이 분비소관을 포함한 세포꼭대기쪽막 에 존재하였으며 콜린성 분비 자극으로 세포꼭대기쪽막으로 이동되어 밀집되었다. AQP5는 모든 침샘의 사이관과 줄무늬 관에도 존재하였으며 점액세포는 약하게 존재하였다. AQP8은 AQP5처럼 장액세포의 바닥쪽막과 세포사이 분비소관을 포함한 세포꼭대기쪽막에 존재하였으며 콜린성 침분비 자극으로 세포꼭대기쪽막쪽으로 이동되어 밀집되었다. 이상의 실험결과는 AQP5와 AQP8이 장액세포에서 일차 침 형성시 일어나는 수분이동의 주된 통로임을 시사하였다. The salivary glands produce 1.5 l of fluid per day. As in other organs, the general paradigm in the salivary glands is that water movement occurs secondary to osmotic driving forces created by active salt transport. Therefore, high water permeability in salivary glands is expected to need a variety of aquaporin (AQP), a water channel. Although four AQPs have been known to reside in salivary glands, the precise location and roles of AQPs have been not well examined. This study is aimed to investigate the distribution of AQPs in 3 major salivary glands and their changes after cholinergic stimulation using immunohistochemical study in Sprague Dawley rats weighing 300 g under pentobarbital sodium anesthesia. AQP1 was localized in the endothelial cells of all salivary capillary vessels and the myoepithelial cells. AQP4 was demonstrated in the epithelium of the excretory ductal cells of all salivary glands. AQP5 and 8 were abundantly present in the basolateral membrane and apical membranes of the serous acini including intercellular secretory canaliculi, whereas AQP5 was weakly present in mucous acini. In addition, AQP5 was found in the epithelium of the intercalated and striated ducts. Upon stimulation of carbachol (10 μg/kg, I.P). AQP5 and 8 tended to translocate from basolateral membrane to the apical membrane, appearing as clusters of dots. These results suggest that AQP5 and 8 are the candidate molecules responsible for the water movement in salivary acinar cells.
Kim, Dong Hwan (Dennis),Lee, Seung-Tae,Won, Hong-Hee,Kim, Seonwoo,Kim, Min-Ji,Kim, Hee-Jin,Kim, Sun-Hee,Kim, Jong-Won,Kim, Hyeoung-Joon,Kim, Yeo-Kyeoung,Sohn, Sang Kyun,Moon, Joon Ho,Jung, Chul Won,Li American Society of Hematology 2011 Blood Vol.117 No.25
<B>Abstract</B><P>In the current study, we identified 2 genetic markers for susceptibility to chronic myeloid leukemia (CML) using a genome-wide analysis. A total of 2744 subjects (671 cases and 2073 controls) were included, with 202 Korean CML patients and 497 control subjects enrolled as a discovery set. Significant findings in the discovery set were validated in a second Korean set of 237 patients and 1000 control subjects and in an additional Canadian cohort of European descent, including 232 patients and 576 control subjects. Analysis revealed significant associations of 2 candidate loci, 6q25.1 and 17p11.1, with CML susceptibility, with the lowest combined P values of 2.4 × 10−6 and 1.3 × 10−12, respectively. Candidate genes in those regions include RMND1, AKAP12, ZBTB2, and WSB1. The locus 6q25.1 was validated in both Korean and European cohorts, whereas 17p11.1 was validated only in the Korean cohort. These findings suggest that genetic variants of 6q25.1 and 17p11.1 may predispose one to the development of CML.</P>
Superparamagnetism of Cu2Se Nanoparticles
Kim, Soo-Whan,Lee, Kyu Joon,Jung, Myung-Hwa,Li, Yan,Seo, Won Seok American Scientific Publishers 2012 Journal of nanoscience and nanotechnology Vol.12 No.7
<P>Cu2Se nanoparticles were synthesized using the standard Schlenk line and glove box techniques, with the hot-injection method. The X-ray diffraction (XRD) analysis showed that the initial nanoparticles were formed in a stoichiometric Cu2Se phase with a cubic structure. When the nanoparticles are exposed to air, the diffraction peaks shift to higher angles. This suggests that the nanoparticles are changed to a nonstoichiometric Cu2-deltaSe phase with copper vacancies. The mean size of the nanoparticles was about 7 nm. The magnetic results show that the initial nanoparticles were diamagnetic, and after 1-week air exposure, they became paramagnetic. This dramatic change from diamagnetic to paramagnetic can be explained by the oxidation of Cu+ into Cu2+ at the nanoparticle surface. In addition, the superparamagnetic properties were observed to have a blocking temperature of 150 K. The coercive field decreased as the temperature approached the blocking temperature, and eventually vanished above the blocking temperature.</P>
Reading Single DNA with DNA Polymerase Followed by Atomic Force Microscopy
Kim, Youngkyu,Kim, Eung-Sam,Lee, Yoonhee,Kim, Joung-Hun,Shim, Bong Chu,Cho, Seong Moon,Lee, Jeong Soo,Park, Joon Won American Chemical Society 2014 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.136 No.39
<P>The importance of DNA sequencing in the life sciences and personalized medicine is continually increasing. Single-molecule sequencing methods have been developed to analyze DNA directly without the need for amplification. Here, we present a new approach to sequencing single DNA molecules using atomic force microscopy (AFM). In our approach, four surface-conjugated nucleotides were examined sequentially with a DNA polymerase-immobilized AFM tip. By observing the specific rupture events upon examination of a matching nucleotide, we could determine the template base bound in the polymerase’s active site. The subsequent incorporation of the complementary base in solution enabled the next base to be read. Additionally, we observed that the DNA polymerase could incorporate the surface-conjugated dGTP when the applied force was controlled by employing the force-clamp mode.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2014/jacsat.2014.136.issue-39/ja5063983/production/images/medium/ja-2014-063983_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja5063983'>ACS Electronic Supporting Info</A></P>
Integrity Evaluation System of CANDU Reactor Pressure Tube
Kim, Young-Jin,Kwak, Sang-Log,Lee, Joon-Seong,Park, Youn-Won The Korean Society of Mechanical Engineers 2003 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.17 No.7
The pressure tube is a major component of the CANDU reactor, which supports nuclear fuel bundle. In order to complete the integrity evaluation of pressure tube, expert knowledge, iterative calculation procedures and a lot of input data are required. More over, results of integrity assessment may be different according to the evaluation method. For this reason, an integrity evaluation system, which provides efficient way of evaluation with the help of attached database, was developed. The present system was built on the basis of 3D FEM results, ASME Sec. XI, and Fitness For Service Guidelines for CANDU pressure tubes issued by the AECL (Atomic Energy Canada Limited). The present system also covers the delayed hydride cracking and the blister evaluation, which are the characteristics of pressure tube integrity evaluation. In order to verify the present system, several case studies have been performed and the results were compared with those from AECL. A good agreement was observed between those two results.
Properties of ZnO thin films co-doped with hydrogen and fluorine.
Kim, Yong Hyun,Kim, Jin Soo,Jeong, Jeung-Hyun,Park, Jong-Keuk,Baik, Young Joon,Lee, Kyeong-Seok,Cheong, Byung-Ki,Kim, Donghwan,Seong, Tae-Yeon,Kim, Won Mok American Scientific Publishers 2012 Journal of nanoscience and nanotechnology Vol.12 No.4
<P>ZnO films co-doped with fluorine and hydrogen were prepared on Corning glass by radio frequency magnetron sputtering of ZnO targets with varying amounts of ZnF2 in H2/Ar gas mixtures of varying H2 content. The ZnO films' electrical, optical, and structural properties in combination with their compositional properties were investigated. A small addition of H2 to the sputtering gas caused a drastic increase of Hall mobility with a marginal increase in carrier concentration, indicating an effective passivation of grain boundaries due to hydrogenation. For further increase of H2 in sputter gas, the Hall mobility remained at a relatively constant level while the carrier concentration increased steadily. Most of the ZnO films co-doped with fluorine and hydrogen showed average transmittance higher than 83% in the 400-800 nm range, while the average absorption coefficients were lower than 600 cm(-1), implying very low absorption loss in these films. It was discovered that the fabrication of ZnO films with a Hall mobility higher than 40 cm2/Vs and a very low absorption loss in the visible range is possible by co-doping hydrogen and fluorine.</P>